BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,201,795	C→A	5.6%	A164S (GCA→TCA) ‡	PP_4577 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,201,795	0	C	A	5.6%	1292.2 / 44.2	383	A164S (GCA→TCA) ‡	PP_4577	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (148/213); new base A (4/18); total (152/231)
Fisher's exact test for biased strand distribution p-value = 4.21e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 389 total aligned reads displayed.

GCCAGACGCACCGCCTGCTCCACCACCCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGTTGTACAGCGCACCATGGGGCTTCACATAGGCCACGC  >  NC_002947/5201647‑5201944

 ccAGACGCACCGCCTGCTCCACCACCCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCt                                                                                                                                                     >  2:4098124/1‑150 (MQ=255)

 ccAGACGCACCGCCTGCTCCACCACCCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCt                                                                                                                                                     <  1:4901685/150‑1 (MQ=255)

 ccAGACGCACCGCCTGCTCCACCACCCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCt                                                                                                                                                     <  1:4232298/150‑1 (MQ=255)

  cAGACGCACCGCCTGCTCCACCACCCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTg                                                                                                                                                    <  1:5958960/150‑1 (MQ=255)

  cAGACGCACCGCCTGCTCCACCACCCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTg                                                                                                                                                    <  1:8732959/150‑1 (MQ=255)

  cAGACGCACCGCCTGCTCCACCACCCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTg                                                                                                                                                    >  1:2299883/1‑150 (MQ=255)

     aCGCACCGCCTGCTCCACCACCCGCGCCGGGTCGTGATGCACCGCACCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGggc                                                                                                                                                 <  1:3831100/150‑1 (MQ=255)

       gCACCGCCTGCTCCACCACCCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGggcgg                                                                                                                                               <  1:4835277/150‑1 (MQ=255)

       gCACCGCCTGCTCCACCACCCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGggcgg                                                                                                                                               >  1:7711012/1‑150 (MQ=255)

       gCACCGCCTGCTCCACCACCCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGggcgg                                                                                                                                               >  2:2266677/1‑150 (MQ=255)

       gCACCGCCTGCTCCACCACCCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGGCACTGggcga                                                                                                                                               <  1:7940667/150‑2 (MQ=255)

       gCACCGCCTGCTCCACCACCCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGGCACTGggcga                                                                                                                                               <  2:7715109/150‑2 (MQ=255)

       gCACCGCCTGCTCCACCACCCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCACCGCCCAGGCACTGggcga                                                                                                                                               <  1:6608556/150‑2 (MQ=255)

       gCACCGCCTGCTCCACCACCCGCGCCGGGTCGTGATGCACCGCGCACGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGACTGCGAACGCCTCGAACCACAGCGGCACGACGATTTCATCGCCCAGTGCATGggcgg                                                                                                                                               >  1:4167266/1‑150 (MQ=255)

        cACCGCCTGCTCCACCACCCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGTCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGGCACTGggcgat                                                                                                                                              <  1:5027994/150‑3 (MQ=255)

        cACCGCCTGCTCCACCACCCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGggcggc                                                                                                                                              >  1:939554/1‑150 (MQ=255)

        cACCGCCTGCTCCACCACCCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGGCACTGggcgat                                                                                                                                              <  1:4797129/150‑3 (MQ=255)

         aCCGCCTGCTCCACCACCCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCa                                                                                                                                             >  2:6738630/1‑150 (MQ=255)

            gCCTGCTCCACCACCCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACgg                                                                                                                                          <  2:1172407/150‑1 (MQ=255)

            gCCTGCTCCACCACCCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACgg                                                                                                                                          <  1:3343872/150‑1 (MQ=255)

                 cTCCACCACCCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTc                                                                                                                                     <  1:3393087/150‑1 (MQ=255)

                  tCCACCACCCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTcg                                                                                                                                    >  1:293381/1‑150 (MQ=255)

                  tCCACCACCCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGAGTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTcg                                                                                                                                    >  2:4409607/1‑150 (MQ=255)

                     accaccCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTcggcg                                                                                                                                 <  2:3067152/150‑1 (MQ=255)

                       caccCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGt                                                                                                                               <  2:4840137/150‑1 (MQ=255)

                        accCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTg                                                                                                                              <  2:2475266/150‑1 (MQ=255)

                          ccGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGc                                                                                                                            >  2:6522340/1‑150 (MQ=255)

                           cgcgCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGcc                                                                                                                           <  2:1929413/150‑1 (MQ=255)

                               ccGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCa                                                                                                                       <  1:6161710/150‑1 (MQ=255)

                                  ggTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCa                                                                                                                    <  2:5686397/150‑1 (MQ=255)

                                  ggTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCa                                                                                                                    <  1:252803/150‑1 (MQ=255)

                                   gTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCAt                                                                                                                   >  2:530541/1‑150 (MQ=255)

                                   gTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCAt                                                                                                                   <  2:6379969/150‑1 (MQ=255)

                                      gTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCaa                                                                                                                >  2:7525241/1‑150 (MQ=255)

                                       tGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAAc                                                                                                               <  1:2598966/150‑1 (MQ=255)

                                          gtcgcggcgcCAGCCGGGCGCCCCCACAGCCCATGGCCGCAGGCAGTGTATGCGCGGTCTGCGAACCCCTCCAACCACAGCGGCACCCCGCATTCATCGCCCACTGCCTGGGCGGCACGGGTGTCGGCGGTGGCCATCAGCATCAACGGc                                                                                                            >  2:7754426/7‑150 (MQ=255)

                                           gCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCa                                                                                                           <  2:1831237/150‑1 (MQ=255)

                                           gCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCa                                                                                                           >  1:8638546/1‑150 (MQ=255)

                                             aCCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAgg                                                                                                         <  1:3632048/150‑1 (MQ=255)

                                             aCCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAgg                                                                                                         <  2:811470/150‑1 (MQ=255)

                                                        cAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATa                                                                                              <  1:4692424/150‑1 (MQ=255)

                                                        cAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATa                                                                                              <  1:2745222/150‑1 (MQ=255)

                                                           gcgcCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGc                                                                                           >  2:6480952/1‑150 (MQ=255)

                                                           gcgcCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGc                                                                                           >  1:2096273/1‑150 (MQ=255)

                                                              ccgcGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCcgc                                                                                        <  1:6738944/150‑1 (MQ=255)

                                                                gcgACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGa                                                                                      <  1:5747628/150‑1 (MQ=255)

                                                                  gACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACg                                                                                    <  1:7145599/150‑1 (MQ=255)

                                                                  gACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACg                                                                                    >  2:6109107/1‑150 (MQ=255)

                                                                   aCAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACgg                                                                                   >  2:152057/1‑150 (MQ=255)

                                                                     agcagATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGcc                                                                                 >  1:2012218/1‑150 (MQ=255)

                                                                      gcagATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGTCGCGGCCt                                                                                <  2:4296311/150‑1 (MQ=255)

                                                                       cagATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTc                                                                               <  2:465091/150‑1 (MQ=255)

                                                                       cagATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTc                                                                               >  1:6461802/1‑150 (MQ=255)

                                                                        agATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGTCGCGGCCtat                                                                              <  2:552700/150‑3 (MQ=255)

                                                                         gATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCa                                                                             <  2:4158484/150‑1 (MQ=255)

                                                                          aTGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAg                                                                            <  2:7427411/150‑1 (MQ=255)

                                                                          aTGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCCGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAg                                                                            <  1:7525341/150‑1 (MQ=255)

                                                                           tGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGTCGCGGCCtatgac                                                                           <  2:388766/150‑6 (MQ=255)

                                                                           tGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGc                                                                           >  1:1380819/1‑150 (MQ=255)

                                                                            ggCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGTCGCGGCCtatgaca                                                                          <  2:2098577/150‑7 (MQ=255)

                                                                              ccGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACg                                                                        >  1:4407034/1‑150 (MQ=255)

                                                                               cGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACgg                                                                       >  2:5206973/1‑150 (MQ=255)

                                                                                     cAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCa                                                                 >  1:4424793/1‑150 (MQ=255)

                                                                                       gtgtATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGc                                                               >  1:829206/1‑150 (MQ=255)

                                                                                         gtATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCtt                                                             <  2:499128/150‑1 (MQ=255)

                                                                                         gtATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCtt                                                             <  1:8114769/150‑1 (MQ=255)

                                                                                                  gTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTc                                                    >  1:4514375/1‑150 (MQ=255)

                                                                                                   tCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCg                                                   <  2:581896/150‑1 (MQ=255)

                                                                                                       cGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCca                                               >  2:2908014/1‑150 (MQ=255)

                                                                                                         aaCGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCcatc                                             <  2:736693/150‑1 (MQ=255)

                                                                                                           cGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCcatcat                                           <  2:2145598/150‑1 (MQ=255)

                                                                                                            gCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATg                                          >  1:4965925/1‑150 (MQ=255)

                                                                                                              cTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTc                                        >  1:2180307/1‑150 (MQ=255)

                                                                                                              cTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGAGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTc                                        >  1:8209591/1‑150 (MQ=255)

                                                                                                                cGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGt                                      <  1:1470106/150‑1 (MQ=255)

                                                                                                                 gAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGtt                                     <  2:343585/150‑1 (MQ=255)

                                                                                                                 gAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGtt                                     <  2:6196645/150‑1 (MQ=255)

                                                                                                                 gAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGtt                                     <  2:8524901/150‑1 (MQ=255)

                                                                                                                 gAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGtt                                     <  2:8443512/150‑1 (MQ=255)

                                                                                                                   aCCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGTTGt                                   <  2:7167704/150‑1 (MQ=255)

                                                                                                                     cacaGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGTTGTAc                                 >  1:6055912/1‑150 (MQ=255)

                                                                                                                      acaGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGTTGTACa                                >  1:3192144/1‑150 (MQ=255)

                                                                                                                      acaGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGTTGTACa                                >  1:1639453/1‑150 (MQ=255)

                                                                                                                      acaGCGGCACGCCGAGTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGTTGTCCa                                >  2:8616088/1‑150 (MQ=255)

                                                                                                                             cACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGTTGTACAGCGCAcc                         >  1:8476147/1‑150 (MQ=255)

                                                                                                                                gCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGTTGTACAGCGCACCATg                      >  2:8783708/1‑150 (MQ=255)

                                                                                                                                gCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGTTGTACAGCGCACCATg                      >  2:1256017/1‑150 (MQ=255)

                                                                                                                                    aTTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGTTGTACAGCGCACCATGGGGc                  <  2:6047225/150‑1 (MQ=255)

                                                                                                                                       tCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGTTGTACAGCGCACCATGGGGCTTc               <  2:3295628/150‑1 (MQ=255)

                                                                                                                                       tCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGTTGTACAGCGCACCATGGGGCTTc               <  1:108439/150‑1 (MQ=255)

                                                                                                                                        cATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGTTGTACAGCGCACCATGGGGCTTca              >  1:838094/1‑150 (MQ=255)

                                                                                                                                         aTCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGTTGTACAGCGCACCATGGGGCTTcac             >  2:5083004/1‑150 (MQ=255)

                                                                                                                                         aTCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCCGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCgg                                                       >  1:5212446/1‑108 (MQ=255)

                                                                                                                                           cGCCCAGGCACTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGTTGTACAGCGCACCATGGGGCTTCACAt           >  1:1029364/1‑150 (MQ=255)

                                                                                                                                            gCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGTTGTACAGCGCACCATGGGGCTTCACATa          <  2:6804311/150‑1 (MQ=255)

                                                                                                                                             cccAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGTTGTACAGCGCACCATGGGGCTTCACATAg         <  2:3777742/150‑1 (MQ=255)

                                                                                                                                             cccAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGTTGTACAGCGCACCATGGGGCTTCACATAg         <  2:2627663/150‑1 (MQ=255)

                                                                                                                                              ccAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGTTGTACAGCGCACCATGGGGCTTCACATAgg        <  2:6982355/150‑1 (MQ=255)

                                                                                                                                              ccAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGTTGTACAGCGCACCATGGGGCTTCACATAgg        >  1:530887/1‑150 (MQ=255)

                                                                                                                                               cAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGTTGTACAGCGCACCATGGGGCTTCACATAGGc       <  1:7808141/150‑1 (MQ=255)

GCCAGACGCACCGCCTGCTCCACCACCCGCGCCGGGTCGTGATGCACCGCGCCCGGCAGGCGCCGCGACAGCAGATGGCCGCTGGCAGTGTATGCGCGGTCTGCGAACGCCTCGAACCACAGCGGCACGCCGATTTCATCGCCCAGTGCCTGGGCGGCACGGTTGTCGGCGGTGGCCATCAGCATCAACGGCAGGTTGCTGTCATAGGCCGCGACGGCCTCCAGCACGGTACGCAGCTTGAGCGGGTCGGCCATCATGTCGTTGTACAGCGCACCATGGGGCTTCACATAGGCCACGC  >  NC_002947/5201647‑5201944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`