BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,245,568	C→G	6.1%	L45V (CTG→GTG)	PP_4618 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,245,568	0	C	G	6.1%	1046.6 / 41.2	311	L45V (CTG→GTG)	PP_4618	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (140/152); new base G (4/15); total (144/167)
Fisher's exact test for biased strand distribution p-value = 3.08e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 316 total aligned reads displayed.

GTGTGTGGAGATACCCCATGAAGCCACGTCAAGCCTGCCTGGCCTGCCTGGAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCGATGATGGACAAGGTACTGCAG  >  NC_002947/5245419‑5245717

gtgtgtGGAGATACCCCATGAAGCCACGTCAAGCCTGCCTGGCCTGCCTGGAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGAcc                                                                                                                                                       <  2:2134180/150‑1 (MQ=255)

    gtgGAGATACCCCATGAAGCCACGTCAAGCCTGCCTGGCCTGCCTGGAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCa                                                                                                                                                   <  1:1550112/150‑1 (MQ=255)

      ggAGATACCCCATGAAGCCACGTCAAGCCTGCCTGGCCTGCCTGGAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAgc                                                                                                                                                 >  2:4424865/1‑150 (MQ=255)

        agaTACCCCATGAAGCCACGTCAAGCCTGCCTGGCCTGCCTGGAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAgcgc                                                                                                                                               >  1:8533139/1‑150 (MQ=255)

         gaTACCCCATGAAGCCACGTCAAGCCTGCCTGGCCTGCCTGGAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCAGGTCGTGCAgcgca                                                                                                                                              <  1:6395725/150‑2 (MQ=255)

         gaTACCCCATGAAGCCACGTCAAGCCTGCCTGGCCTGCCTGGAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCAGGTCGTGCAgcgca                                                                                                                                              <  1:3905805/150‑2 (MQ=255)

            aCCCCATGAAGCCACGTCAAGCCTGCCTGGCCTGCCTGGAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCgaga                                                                                                                                           <  1:2745001/150‑1 (MQ=255)

              cccATGAAGCCACGTCAAGCCTGCCTGGCCTGCCTGGAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATa                                                                                                                                         >  2:5804281/1‑150 (MQ=255)

              cccATGAAGCCACGTCAAGCCTGCCTGGCCTGCCTGGAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATa                                                                                                                                         >  1:7002551/1‑150 (MQ=255)

                 aTGAAGCCACGTCAAGCCTGCCTGGCCTGCCTGGAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAgc                                                                                                                                      <  1:3431010/150‑1 (MQ=255)

                 aTGAAGCCACGTCAAGCCTGCCTGGCCTGCCTGGAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGAGTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAgc                                                                                                                                      <  1:1496418/150‑1 (MQ=255)

                  tGAAGCCACGTCAAGCCTGCCTGGCCTGCCTGGAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAgcg                                                                                                                                     <  1:983821/150‑1 (MQ=255)

                      gCCACGTCAAGCCTGCCTGGCCTGCCTGGAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCaa                                                                                                                                 >  2:3709639/1‑150 (MQ=255)

                       ccACGTCAAGCCTGCCTGGCCTGCCTGGAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAAc                                                                                                                                >  2:1672589/1‑150 (MQ=255)

                       ccACGTCAAGCCTGCCTGGCCTGCCTGGAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAAc                                                                                                                                >  1:7293375/1‑150 (MQ=255)

                         aCGTCAAGCCTGCCTGGCCTGCCTGGAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCt                                                                                                                              >  1:3510548/1‑150 (MQ=255)

                         aCGTCAAGCCTGCCTGGCCTGCCTGGAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCt                                                                                                                              >  1:1578326/1‑150 (MQ=255)

                               aGCCTGCCTGGCCTGCCTGGAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGAt                                                                                                                        <  2:517253/150‑1 (MQ=255)

                                gcctgcctggcctgcctgGAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATg                                                                                                                       >  2:2460062/1‑150 (MQ=255)

                                 cctgcctggcctgcctggAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGACGATGc                                                                                                                      >  2:2360649/1‑150 (MQ=255)

                                    gcctggcctgcctggAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTc                                                                                                                   >  1:4296234/1‑150 (MQ=255)

                                     cctggcctgcctggAACGCGAGCCTGTCGCCCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTcc                                                                                                                  <  2:5651704/150‑1 (MQ=255)

                                      ctggcctgcctggAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCa                                                                                                                 <  2:4733738/150‑1 (MQ=255)

                                      ctggcctgcctggAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCa                                                                                                                 <  2:1358696/150‑1 (MQ=255)

                                       tggcctgcctggAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCAc                                                                                                                >  1:114485/1‑150 (MQ=255)

                                           ctgcctggAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATg                                                                                                            <  1:4506124/150‑1 (MQ=255)

                                            tgcctggAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGc                                                                                                           <  2:4525732/150‑1 (MQ=255)

                                            tgcctggAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGc                                                                                                           <  2:8177914/150‑1 (MQ=255)

                                             gcctggAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCg                                                                                                          <  2:6132830/150‑1 (MQ=255)

                                              cctggAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGa                                                                                                         <  2:7002551/150‑1 (MQ=255)

                                               ctggAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGaa                                                                                                        <  2:8374943/150‑1 (MQ=255)

                                                    aCGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGc                                                                                                   <  2:3643919/150‑1 (MQ=255)

                                                     cgcgAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGcc                                                                                                  <  2:6639700/150‑1 (MQ=255)

                                                      gcgAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGccc                                                                                                 <  1:6912493/150‑1 (MQ=255)

                                                             tGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCAct                                                                                          >  1:6875683/1‑150 (MQ=255)

                                                               tCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCActgc                                                                                        <  2:2997931/150‑1 (MQ=255)

                                                               tCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCActgc                                                                                        >  2:3176875/1‑150 (MQ=255)

                                                                     tgctgGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGcc                                                                                  >  1:5304853/1‑150 (MQ=255)

                                                                     tgctgGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGcc                                                                                  >  2:1991119/1‑150 (MQ=255)

                                                                     tgctgGAAGCCGCACTGTGGAGTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGcc                                                                                  >  2:8700151/1‑150 (MQ=255)

                                                                      gctgGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCa                                                                                 <  2:4923669/150‑1 (MQ=255)

                                                                        tgGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGc                                                                               <  1:6144192/150‑1 (MQ=255)

                                                                        tgGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGc                                                                               <  2:114485/150‑1 (MQ=255)

                                                                         ggAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAACt                                                                              <  2:2800332/150‑1 (MQ=255)

                                                                            aGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGaa                                                                           <  2:2561673/150‑1 (MQ=255)

                                                                             gCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAAt                                                                          <  2:3377943/150‑1 (MQ=255)

                                                                                   cTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTg                                                                    <  1:7938097/150‑1 (MQ=255)

                                                                                   cTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTg                                                                    <  2:6558697/150‑1 (MQ=255)

                                                                                    tggggATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTgg                                                                   >  2:7160775/4‑150 (MQ=255)

                                                                                     gtgGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTggg                                                                  <  1:231488/150‑1 (MQ=255)

                                                                                        gATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCtt                                                               >  1:7666137/1‑150 (MQ=255)

                                                                                         aTTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCttt                                                              <  2:3305102/150‑1 (MQ=255)

                                                                                          ttGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTc                                                             <  1:8685521/150‑1 (MQ=255)

                                                                                          ttGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTc                                                             <  2:8533139/150‑1 (MQ=255)

                                                                                           tgctgcCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTca                                                            <  1:1005356/150‑1 (MQ=255)

                                                                                            gctgcCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTcag                                                           >  1:3680962/1‑150 (MQ=255)

                                                                                              tgcCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTcagca                                                         <  1:1672589/150‑1 (MQ=255)

                                                                                              tgcCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTcagca                                                         >  2:5025548/1‑150 (MQ=255)

                                                                                              tgcCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTcagca                                                         >  2:8231072/1‑150 (MQ=255)

                                                                                              tgcCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTcagca                                                         <  2:1323097/150‑1 (MQ=255)

                                                                                              tgcCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTcagca                                                         >  1:8091845/1‑150 (MQ=255)

                                                                                               gcCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTcagcag                                                        <  2:3516295/150‑1 (MQ=255)

                                                                                                 cGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGa                                                      <  1:1979131/150‑1 (MQ=255)

                                                                                                  gAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGAt                                                     >  1:2498866/1‑150 (MQ=255)

                                                                                                      aCGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAat                                                 <  2:2401926/150‑1 (MQ=255)

                                                                                                          cccTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCa                                             >  2:7589755/1‑150 (MQ=255)

                                                                                                             tGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCAccc                                          >  1:5408887/1‑150 (MQ=255)

                                                                                                             tGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCAccc                                          >  1:748584/1‑150 (MQ=255)

                                                                                                              ggTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCAcccc                                         <  2:2303632/150‑1 (MQ=255)

                                                                                                                     aGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGcc                                  >  1:7728763/1‑150 (MQ=255)

                                                                                                                      gCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGccc                                 <  1:5229964/150‑1 (MQ=255)

                                                                                                                        ccGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGAGGAATATCACCCCTTGCGccccc                               >  2:8590675/1‑150 (MQ=255)

                                                                                                                         cGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCCCTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCa                              >  2:3975202/1‑150 (MQ=255)

                                                                                                                         cGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATTACCCCTTGCGCCCCCa                              >  1:7257681/1‑150 (MQ=255)

                                                                                                                            tGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATgc                           >  1:4134717/1‑150 (MQ=255)

                                                                                                                                aGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATgcggcg                       >  1:301978/1‑150 (MQ=255)

                                                                                                                                aGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATgcggcg                       >  1:3108301/1‑150 (MQ=255)

                                                                                                                                 gCCTTGCTGCGCTGCACGACCTGCAGCGCGGGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCga                      >  1:402696/1‑150 (MQ=255)

                                                                                                                                 gCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCga                      <  1:2214311/150‑1 (MQ=255)

                                                                                                                                 gCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCga                      <  1:4028344/150‑1 (MQ=255)

                                                                                                                                  ccTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCgat                     <  1:3354043/150‑1 (MQ=255)

                                                                                                                                  ccTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCgat                     >  2:2640131/1‑150 (MQ=255)

                                                                                                                                   cTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCgatg                    <  2:7321279/150‑1 (MQ=255)

                                                                                                                                    ttGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCgatga                   >  1:2948151/1‑150 (MQ=255)

                                                                                                                                    ttGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCgatga                   >  1:4997877/1‑150 (MQ=255)

                                                                                                                                      gctgcgctgcACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCGatgatg                 <  2:4466895/150‑1 (MQ=255)

                                                                                                                                      gctgcgctgcACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCGatgatg                 <  1:8397920/150‑1 (MQ=255)

                                                                                                                                      gctgcgctgcACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCGatgatg                 <  1:6817577/150‑1 (MQ=255)

                                                                                                                                      ctcgcgctgcAGGTCGTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCGatgatg                 >  2:5496646/4‑150 (MQ=255)

                                                                                                                                       tcgcgctgcAGGTCGTGCAGCGCAAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCGATGATgg                >  2:1834996/3‑150 (MQ=255)

                                                                                                                                       ctgcgctgcACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCGATGATgg                >  1:4767362/1‑150 (MQ=255)

                                                                                                                                        tgcgctgcACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCGATGATGGa               >  1:3189086/1‑150 (MQ=255)

                                                                                                                                        tgcgctgcACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCGATGATGGa               <  1:5476670/150‑1 (MQ=255)

                                                                                                                                           gctgcAGGTCGTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCGATGATGGAcca            >  2:7923391/1‑148 (MQ=255)

                                                                                                                                           gctgcACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCGATGATGGACaa            <  2:7253228/150‑1 (MQ=255)

                                                                                                                                            ctgcACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCGATGATGGACAAg           <  1:439235/150‑1 (MQ=255)

                                                                                                                                                  gACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCGATGATGGACAAGGTACTg     >  2:1562915/1‑150 (MQ=255)

                                                                                                                                                  gACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCGATGATGGACAAGGTACTg     >  1:8002533/1‑150 (MQ=255)

                                                                                                                                                   aCCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCGATGATGGACAAGGTACTGc    <  2:3858980/150‑1 (MQ=255)

                                                                                                                                                    ccTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCGATGATGGACAAGGTACTGCa   <  1:5735822/150‑1 (MQ=255)

GTGTGTGGAGATACCCCATGAAGCCACGTCAAGCCTGCCTGGCCTGCCTGGAACGCGAGCCTGTCGACCTGCTGGAAGCCGCACTGTGGATTGCTGCCGAGCACGACCCTGGTGTCGAGCCCGCTGCCAGCCTTGCTGCGCTGCACGACCTGCAGCGCGAGATAAGCGCCAACCTGCCGATGCTTCCACTATGCGAACTGGCCCAGCCACTGCTGCGCCAGCTGAATGCGCTGGGCTTTCAGCAGGATGAATATCACCCCTTGCGCCCCCATGCGGCGATGATGGACAAGGTACTGCAG  >  NC_002947/5245419‑5245717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`