BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,366,069	A→T	5.1%	intergenic (‑79/+22)	PP_t49 ← / ← PP_t48	tRNA‑Met/tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,366,069	0	A	T	5.1%	1518.9 / 42.7	444	intergenic (‑79/+22)	PP_t49/PP_t48	tRNA‑Met/tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base A (204/215); new base T (11/12); total (216/228)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 447 total aligned reads displayed.

GGGGGCTGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCTTGAGCCGG  >  NC_002947/5365921‑5366218

gggggCTGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTaaa                                                                                                                                                      <  1:3887403/150‑1 (MQ=255)

   ggCTGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCg                                                                                                                                                   <  2:3436394/150‑1 (MQ=255)

    gCTGGATTTGAACCCACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCCCGACAAAGCTGGGGCAAAAGTATACGACCGAACCATGTTGAGGGTCAAAGCCAAACATGCACCTACAAGAAAGCCCGCTAAAGCgg                                                                                                                                                  <  2:8814648/150‑1 (MQ=38)

    gCTGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCgg                                                                                                                                                  <  1:7635232/150‑1 (MQ=255)

        gATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTTTAGCGGGctt                                                                                                                                              <  2:2043686/150‑2 (MQ=255)

         aTTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATACGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTTTAGCGGGcttt                                                                                                                                             <  1:2610820/150‑3 (MQ=255)

          tttGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCtttc                                                                                                                                            <  1:6473614/150‑1 (MQ=255)

           ttGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCtctct                                                                                                                                           <  2:2580036/150‑2 (MQ=255)

            tGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCaa                                                                                                                                          <  2:4285020/150‑1 (MQ=255)

             gAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAg                                                                                                                                         <  1:6854166/150‑1 (MQ=255)

             gAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAg                                                                                                                                         <  2:7013261/150‑1 (MQ=255)

               aCCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCt                                                                                                                                       <  1:1988866/150‑1 (MQ=255)

                ccAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACACGAGAGCCCGCTTTAGCGGGCtttcttgtag                                                                                                                                      <  2:5996820/150‑7 (MQ=38)

                ccAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAGAGCCCGCTTTAGCGGGCtttcttgtag                                                                                                                                      <  2:1734831/150‑7 (MQ=38)

                  aaCGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTa                                                                                                                                    >  2:2200944/1‑150 (MQ=255)

                  aaCGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTa                                                                                                                                    >  1:2944262/1‑150 (MQ=255)

                    cGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAAt                                                                                                                                  <  1:376384/150‑1 (MQ=255)

                       ccTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTgg                                                                                                                               <  1:5551839/150‑1 (MQ=255)

                       cattCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTgg                                                                                                                               <  1:792625/148‑1 (MQ=255)

                        cTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGt                                                                                                                              <  1:69954/150‑1 (MQ=255)

                        cTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGt                                                                                                                              <  1:4483285/150‑1 (MQ=255)

                         ttCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTa                                                                                                                             <  2:5352914/150‑1 (MQ=255)

                          tCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTAc                                                                                                                            >  2:3544024/1‑150 (MQ=255)

                           cGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAACGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTAcc                                                                                                                           <  1:7502207/150‑1 (MQ=255)

                           cGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTAcc                                                                                                                           <  1:312016/150‑1 (MQ=255)

                             ggTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTAGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCga                                                                                                                         <  2:2711395/150‑1 (MQ=255)

                              gTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCgag                                                                                                                        >  1:680601/1‑150 (MQ=255)

                                tATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGaa                                                                                                                      <  2:7870466/150‑1 (MQ=255)

                                   gAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAggg                                                                                                                   <  1:1452649/150‑1 (MQ=255)

                                             aGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAAcc                                                                                                         <  2:4982082/150‑1 (MQ=255)

                                              gCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAAccc                                                                                                        <  1:4862328/150‑1 (MQ=255)

                                               cTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAAcccc                                                                                                       <  2:4371916/150‑1 (MQ=255)

                                                tACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCt                                                                                                      >  2:5861183/1‑150 (MQ=255)

                                                   cAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTaca                                                                                                   >  2:8002528/1‑150 (MQ=255)

                                                    aaGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTacac                                                                                                  >  2:1086139/1‑150 (MQ=255)

                                                     aGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACAcc                                                                                                 <  2:8488666/150‑1 (MQ=255)

                                                          ctCCACCCCTCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACTAGAAATCCCGCTAATGCGGGCTCTCAAGCTTTAATTTTTACGGAGAAGGGGACTCTAACCCCTACACCCTATg                                                                                            <  2:6494877/150‑1 (MQ=255)

                                                          ctCCACCCCGCGACAAAGCTGGGGCAAAACTCTCCGACCGAACCCTGTTGAGGGTCAAATCCAACCATTCACCTACAAGCAAGCCCGCTACAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATg                                                                                            <  2:5230769/150‑1 (MQ=255)

                                                           tCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATgg                                                                                           >  1:5876434/1‑150 (MQ=255)

                                                           tCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATgg                                                                                           >  2:3979833/1‑150 (MQ=255)

                                                           tCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATgg                                                                                           >  1:7743128/1‑150 (MQ=255)

                                                             cACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGc                                                                                         >  2:7599421/1‑150 (MQ=255)

                                                              aCCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGca                                                                                        >  1:1821174/1‑150 (MQ=255)

                                                                 ccGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACaa                                                                                     <  2:3791597/150‑1 (MQ=255)

                                                                     gACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAaccac                                                                                 >  2:1542167/1‑150 (MQ=255)

                                                                      aCAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAaccacc                                                                                <  1:8281888/150‑1 (MQ=255)

                                                                        aaaGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTc                                                                              >  1:4572791/1‑150 (MQ=255)

                                                                           gCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAg                                                                           <  1:1125172/150‑1 (MQ=255)

                                                                           gCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAg                                                                           >  1:814192/1‑150 (MQ=255)

                                                                           gCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAg                                                                           <  1:5233423/150‑1 (MQ=255)

                                                                                 gCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCg                                                                     >  1:5814362/1‑150 (MQ=255)

                                                                                  cAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCgt                                                                    <  1:1668715/150‑1 (MQ=255)

                                                                                  cAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCgt                                                                    <  2:6809961/150‑1 (MQ=255)

                                                                                   aaaaGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAGGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCgtg                                                                   >  1:946757/1‑150 (MQ=255)

                                                                                      aGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCt                                                                <  1:204987/150‑1 (MQ=255)

                                                                                       gTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTa                                                               <  2:1458206/150‑1 (MQ=255)

                                                                                        tataAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTAc                                                              >  1:8058651/1‑150 (MQ=255)

                                                                                         ataAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTAcc                                                             >  1:5762383/1‑150 (MQ=255)

                                                                                          taAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCa                                                            >  1:8673174/1‑150 (MQ=255)

                                                                                          taAGACCGAACCCTGTTGAGGGTCAAATCCAAACAGTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCa                                                            >  1:4009878/1‑150 (MQ=255)

                                                                                            aGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAAt                                                          >  2:6572231/1‑150 (MQ=255)

                                                                                            aGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAAt                                                          <  2:4499421/150‑1 (MQ=255)

                                                                                             gACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACTAAtt                                                         >  1:2522256/1‑150 (MQ=255)

                                                                                              aCCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTc                                                        >  1:2709492/1‑150 (MQ=255)

                                                                                              aCCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTc                                                        >  2:6335393/1‑150 (MQ=255)

                                                                                                 gAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTccac                                                     <  2:5644232/150‑1 (MQ=255)

                                                                                                  aaCCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTccacc                                                    >  1:3212596/1‑150 (MQ=255)

                                                                                                  aaCCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTccacc                                                    >  2:3044847/1‑150 (MQ=255)

                                                                                                  aaCCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTccacc                                                    >  2:5666296/1‑150 (MQ=255)

                                                                                                     ccTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGaa                                                                                                           >  1:843201/1‑92 (MQ=255)

                                                                                                     ccTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCaccacc                                                 >  2:3358629/1‑150 (MQ=255)

                                                                                                             gggTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAAt                                         >  2:2452576/1‑150 (MQ=255)

                                                                                                                tCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGGGTGTCTACCAATTCCACCACCTCGGCAAtact                                      >  2:8311797/1‑150 (MQ=255)

                                                                                                                 cAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAAtacta                                     <  1:3442260/150‑1 (MQ=255)

                                                                                                                   aaTCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAAtactact                                   >  2:8229453/1‑150 (MQ=255)

                                                                                                                     tCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAAtactactac                                 <  1:7988217/150‑1 (MQ=255)

                                                                                                                       cAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTAACGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACtt                               <  2:7500184/150‑1 (MQ=255)

                                                                                                                        aaaCATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTg                              >  1:5401711/1‑150 (MQ=255)

                                                                                                                        aaaCATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTg                              >  2:1288505/1‑150 (MQ=255)

                                                                                                                         aaCATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGa                             <  1:2401303/150‑1 (MQ=255)

                                                                                                                          aCATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGaa                            >  1:1008479/1‑150 (MQ=255)

                                                                                                                              tCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAAccc                        >  1:8638394/1‑150 (MQ=255)

                                                                                                                                aCCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAGGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGt                      <  2:946757/150‑1 (MQ=255)

                                                                                                                                   tACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTAc                   >  1:358386/1‑150 (MQ=255)

                                                                                                                                   tACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTAc                   >  1:1334930/1‑150 (MQ=255)

                                                                                                                                   tACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTAc                   >  1:6591264/1‑150 (MQ=255)

                                                                                                                                   tACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTAc                   >  2:8556889/1‑150 (MQ=255)

                                                                                                                                      ttgagaGCCCGCTTTAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTc                >  2:6159/6‑150 (MQ=255)

                                                                                                                                      aaGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACt                                                                                                               <  1:7195552/55‑1 (MQ=255)

                                                                                                                                      aaGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACt                                                                                                               >  2:7195552/1‑55 (MQ=255)

                                                                                                                                      aaGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAac                                                                                    <  2:230160/82‑1 (MQ=255)

                                                                                                                                      aaGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTc                >  2:7713009/1‑150 (MQ=255)

                                                                                                                                        gAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTg              >  2:5590090/1‑150 (MQ=255)

                                                                                                                                        gAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTg              >  1:6333045/1‑150 (MQ=255)

                                                                                                                                             cccGCTTTAGCGGGCTTTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCtt         >  2:3728328/1‑150 (MQ=255)

                                                                                                                                              ccGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAacca                                                                                  >  1:4675076/1‑76 (MQ=255)

                                                                                                                                               cGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCTTGa       <  1:3840965/150‑1 (MQ=255)

                                                                                                                                                gCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCACTACTACTACTTGAAACCCGTTACTTCTGCTCTTGAg      >  1:4270191/1‑150 (MQ=255)

                                                                                                                                                 cTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCCAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCTTGAGc     <  1:1542167/150‑1 (MQ=255)

                                                                                                                                                    aaGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGAACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCTTGAGCCgg  >  2:4773193/1‑150 (MQ=255)

GGGGGCTGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCTTGAGCCGG  >  NC_002947/5365921‑5366218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`