BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,372,780	A→T	5.6%	K102M (AAG→ATG) ‡	PP_4720 →	RNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,372,780	0	A	T	5.6%	1194.6 / 41.5	374	K102M (AAG→ATG) ‡	PP_4720	RNA‑binding protein
Reads supporting (aligned to +/- strand): ref base A (160/192); new base T (14/7); total (174/200)
Fisher's exact test for biased strand distribution p-value = 7.20e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.46e-01

Only 100 of 384 total aligned reads displayed.

CTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTAGCGCAAGGTGTAATAC  >  NC_002947/5372631‑5372929

cTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACaa                                                                                                                                                       <  1:2556828/150‑1 (MQ=255)

     aaGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAAc                                                                                                                                                  <  2:8375640/150‑1 (MQ=255)

         ccGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACTTGTAACGGtg                                                                                                                                              <  2:1583033/150‑2 (MQ=255)

          cgcgAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACTTGTAACGGtgg                                                                                                                                             <  1:1600421/150‑3 (MQ=255)

          cgcgAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAg                                                                                                                                             <  2:7987579/150‑1 (MQ=255)

          cgcgAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAg                                                                                                                                             >  1:1459995/1‑150 (MQ=255)

           gcgAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGc                                                                                                                                            >  1:5983310/1‑150 (MQ=255)

              aagaaCGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTcc                                                                                                                                         <  1:5109603/150‑1 (MQ=255)

                gaaCGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGa                                                                                                                                       <  2:6888829/150‑1 (MQ=255)

                 aaCGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGAt                                                                                                                                      <  1:6223203/150‑1 (MQ=255)

                    gcgcCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAg                                                                                                                                   >  1:6776946/1‑150 (MQ=255)

                        cGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGgc                                                                                                                               >  2:1960268/1‑150 (MQ=255)

                          tgaCCATCGCCGAGGTGGGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCCTCGGCGAGAAAGCCCTGATCTACCGCAAGTACCCGCAGCCGAACAAGCTGCTGTCCATCATCAACCGTTACAAGTACCGGTAGCTCCGATCCGTggggg                                                                                                                             >  2:7825490/2‑147 (MQ=255)

                              cATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTg                                                                                                                         >  1:2747376/1‑150 (MQ=255)

                               aTCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGa                                                                                                                        <  1:4444203/150‑1 (MQ=255)

                                tCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGaa                                                                                                                       >  2:743782/1‑150 (MQ=255)

                                    cGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAAcgcg                                                                                                                   >  1:5494615/1‑150 (MQ=255)

                                    cGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAAcgcg                                                                                                                   >  1:6570388/1‑150 (MQ=255)

                                    cGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAAcgcg                                                                                                                   >  2:56030/1‑150 (MQ=255)

                                            gCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGAcc                                                                                                           >  1:6306120/1‑150 (MQ=255)

                                             cAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGTTCACCGCGccactgatc                                                                                                          <  1:6679835/150‑8 (MQ=255)

                                             cAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGTTCACCGCGccactgatc                                                                                                          <  1:3036510/150‑8 (MQ=255)

                                             cAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGTTCACCGCGccactgatc                                                                                                          <  1:5889393/150‑8 (MQ=255)

                                             cAAGGCCGGCCGGGCCGAGATGGTGCAGACCATCGGCAAGAAAGCCCTGAGCTACCGCAAGAACCCACAGCCGACCACGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCTATCAGTGGCGCGGTGAACGCGCCCTGAcct                                                                                                          <  1:6979587/150‑2 (MQ=255)

                                                ggccggccGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTa                                                                                                       >  1:2880394/1‑150 (MQ=255)

                                                  ccggccgTGCCGGGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTAcc                                                                                                     >  1:1248299/1‑150 (MQ=255)

                                                  caggacttGCCGAGCTGTTGCAGACCATCGTCAATAAAGCCCTGATCTACCTCAATAACCCACAGACGAAAAAGCAGCTTTCCAACATCCACCTTTACAAGTAACGGTAGCTCCTATCAGTGGCGCTGTGAACGCGCCATGAACGGTAcc                                                                                                     <  2:310531/143‑1 (MQ=255)

                                                        gTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCACCATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTgg                                                                                               <  2:4880903/150‑1 (MQ=255)

                                                          gCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGc                                                                                             <  1:8484768/150‑1 (MQ=255)

                                                          gCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGc                                                                                             >  1:5539208/1‑150 (MQ=255)

                                                             gAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACCGCCGAACAAGCAGCTGCCCAACATCCACCGTTACAAGTACCGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGccac                                                                                          <  1:18646/150‑1 (MQ=255)

                                                              aGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGccacc                                                                                         >  1:541418/1‑150 (MQ=255)

                                                               gCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGccacca                                                                                        <  1:7805975/150‑1 (MQ=255)

                                                                     gCAGACCATCGGCAAGAAAGCCCTGATCTACCGCACGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGa                                                                                  <  2:5334882/150‑1 (MQ=255)

                                                                      cAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGAt                                                                                 <  2:7289731/150‑1 (MQ=255)

                                                                      cAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGAt                                                                                 <  2:6473194/150‑1 (MQ=255)

                                                                        gACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGc                                                                               >  2:5942608/1‑150 (MQ=255)

                                                                         aCCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGCCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGcc                                                                              <  1:3410727/150‑1 (MQ=255)

                                                                          ccATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCg                                                                             >  2:4623559/1‑150 (MQ=255)

                                                                           cATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCgg                                                                            >  2:8796196/1‑150 (MQ=255)

                                                                            aTCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGa                                                                           >  2:1259869/1‑150 (MQ=255)

                                                                             tCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGaa                                                                          >  2:5003401/1‑150 (MQ=255)

                                                                             tCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGaa                                                                          >  1:4283358/1‑150 (MQ=255)

                                                                               ggCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTACCGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGaaaa                                                                        <  1:785109/150‑1 (MQ=255)

                                                                                gCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAg                                                                       >  2:6978380/1‑150 (MQ=255)

                                                                                gCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAg                                                                       >  1:4713700/1‑150 (MQ=255)

                                                                                 cAATAAAGCCCTGATCTACCGCAAGAACCCCCAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCggtcaggg                                                                      <  1:2182245/150‑7 (MQ=255)

                                                                                  aaGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGcc                                                                     >  1:8084199/1‑150 (MQ=255)

                                                                                    gAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCa                                                                   <  2:7738540/150‑1 (MQ=255)

                                                                                         cccTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCCGCAcc                                                              >  1:3618269/1‑150 (MQ=255)

                                                                                         cccTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAACACGATGCCGGAAAAGCCCAGCAcc                                                              >  2:5216553/1‑150 (MQ=255)

                                                                                          ccTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCa                                                             <  2:6624571/150‑1 (MQ=255)

                                                                                          ccTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCa                                                             <  2:2964223/150‑1 (MQ=255)

                                                                                           cTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAg                                                            >  2:5495472/1‑150 (MQ=255)

                                                                                           cTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAg                                                            >  1:1120946/1‑150 (MQ=255)

                                                                                              aTCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGaa                                                         <  2:2783910/150‑1 (MQ=255)

                                                                                               tCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCTTTACAAGTAACGGTAGCTCCTATCTGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAg                                                        <  1:1565388/150‑1 (MQ=255)

                                                                                                cTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGc                                                       >  1:2003997/1‑150 (MQ=255)

                                                                                                cTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGc                                                       >  1:4409410/1‑150 (MQ=255)

                                                                                                 tACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAAGGGTAGCTCCTATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCa                                                      <  1:3297938/150‑1 (MQ=255)

                                                                                                   ccGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGTAGa                                                    <  2:3795206/150‑1 (MQ=255)

                                                                                                   ccGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGa                                                    >  2:4689922/1‑150 (MQ=255)

                                                                                                    cGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACAGCCACCGGTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGaa                                                   <  2:2315427/150‑1 (MQ=255)

                                                                                                     gCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAAc                                                  <  1:5473767/150‑1 (MQ=255)

                                                                                                      cAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACa                                                 >  2:135426/1‑150 (MQ=255)

                                                                                                      cAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACa                                                 >  2:4334892/1‑150 (MQ=255)

                                                                                                          aaCCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGACCATCTg                                             >  1:4828883/1‑150 (MQ=255)

                                                                                                           aCCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGc                                            <  2:2034298/150‑1 (MQ=255)

                                                                                                             ccACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGc                                                       <  2:357087/137‑1 (MQ=255)

                                                                                                               acaGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAgc                                        >  1:93749/1‑150 (MQ=255)

                                                                                                                 aGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAgcgc                                      >  2:7116748/1‑150 (MQ=255)

                                                                                                                  gCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCt                                     >  2:1426200/1‑150 (MQ=255)

                                                                                                                     gAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGc                                  <  2:8198841/150‑1 (MQ=255)

                                                                                                                      aaCAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGcc                                 >  1:6431030/1‑150 (MQ=255)

                                                                                                                          agcagcTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACg                             <  1:2466038/150‑1 (MQ=255)

                                                                                                                           gcagcTGCCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACgg                            >  1:7122378/1‑150 (MQ=255)

                                                                                                                           gcagcGGTCCAACCTCCACCGTTACAAGTACCGGTAGCTCCGATCAGGGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACgg                            <  1:8274657/150‑1 (MQ=255)

                                                                                                                             agcTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCTCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGACGCAGAACATCTGCCAGCGCTCGCCCACGGaa                          >  1:8420410/1‑150 (MQ=255)

                                                                                                                             agcTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGaa                          >  1:1754810/1‑150 (MQ=255)

                                                                                                                             agcTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGaa                          <  2:1817575/150‑1 (MQ=255)

                                                                                                                             agcTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGaa                          >  2:1612477/1‑150 (MQ=255)

                                                                                                                                tGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATa                       <  2:4487983/150‑1 (MQ=255)

                                                                                                                                tGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATa                       <  2:6306120/150‑1 (MQ=255)

                                                                                                                                    cAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGt                   >  1:1020242/1‑150 (MQ=255)

                                                                                                                                     aaCATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGTCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTa                  <  2:3886392/150‑1 (MQ=255)

                                                                                                                                     aaCATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGACATACCGTa                  >  2:3962543/1‑150 (MQ=255)

                                                                                                                                     aaCATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTa                  <  1:743782/150‑1 (MQ=255)

                                                                                                                                         tCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTAgcgc              >  2:7583606/1‑150 (MQ=255)

                                                                                                                                         tCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTAgcgc              >  1:104530/1‑150 (MQ=255)

                                                                                                                                           taCCGTTACTTGTAACGGTGGCTCCGATCAGTGGCGCGGTGAACACGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTAGCGCaa            >  1:4085946/2‑150 (MQ=255)

                                                                                                                                           cACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTAGCGCaa            <  1:1975762/150‑1 (MQ=255)

                                                                                                                                            aCCGTTACTTGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGaa                          <  2:5712832/135‑1 (MQ=255)

                                                                                                                                            aCCGTTACTTGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGaa                          >  1:5712832/1‑135 (MQ=255)

                                                                                                                                            aCCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTTAACGCGCCCTGACAGGTACCGGCTGGGCCACCAGAAAGATGCCGTAAAAGACAAGCACAAGGAAGCAGAACATCTGCAAGCGCTCGCCCACGGAAATAACGTAGCGCacg           >  1:3168307/1‑148 (MQ=255)

                                                                                                                                              cGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTAGCGCAAGgt         <  2:4925051/150‑1 (MQ=255)

                                                                                                                                              cGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTAGCGCAAGgt         <  2:1144370/150‑1 (MQ=255)

                                                                                                                                              cGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTAGCGCAAGgt         >  2:8635516/1‑150 (MQ=255)

                                                                                                                                                    aaGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCCCCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGACATACCGTCGCGCACGGTGTAATa   >  1:5237444/1‑150 (MQ=255)

                                                                                                                                                    aaGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTAGCGCAAGGTGTAATa   >  2:3317642/1‑150 (MQ=255)

                                                                                                                                                    aaGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTAGCGCAAGGTGTAATa   >  1:8750722/1‑150 (MQ=255)

CTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGTCCAACATCCACCGTTACAAGTAACGGTAGCTCCGATCAGTGGCGCGGTGAACGCGCCCTGACCGGTACCGGCTGGGCCACCAGCACGATGCCGGAAAAGCCCAGCACCAGGAAGCAGAACATCTGCCAGCGCTCGCCCACGGAAATACCGTAGCGCAAGGTGTAATAC  >  NC_002947/5372631‑5372929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`