Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,393,880 T→G 5.1% L798R (CTG→CGG) ‡ dld2 → D‑lactate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,393,8800TG 5.1% 1160.2 / 34.2 339L798R (CTG→CGG) ‡dld2D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (135/185);  new base G (10/7);  total (146/193)
Fisher's exact test for biased strand distribution p-value = 2.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01

Only 100 of 341 total aligned reads displayed.

CAGCCTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGGGCGAAAGCC  >  NC_002947/5393731‑5394029
                                                                                                                                                     |                                                                                                                                                     
  gCCTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTgg                                                                                                                                                     <  1:717273/150‑1 (MQ=255)
  gCCTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTgg                                                                                                                                                     <  2:3398702/150‑1 (MQ=255)
   ccTGTGCTGCGGCCATCCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGt                                                                                                                                                    <  2:6123163/150‑1 (MQ=255)
      gtgCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCa                                                                                                                                                 <  2:5397668/150‑1 (MQ=255)
      gtgCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCAGGGTGCa                                                                                                                                                 <  1:7739418/150‑1 (MQ=255)
       tgctgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAg                                                                                                                                                <  1:8305647/150‑1 (MQ=255)
       tgctgcGGCCAACCGGTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAg                                                                                                                                                <  2:7337267/150‑1 (MQ=255)
        gctgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGACAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAgg                                                                                                                                               <  1:1769283/150‑1 (MQ=255)
         ctgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCAGGGTGCAggg                                                                                                                                              <  1:8662747/150‑2 (MQ=255)
          tgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGAc                                                                                                                                             <  2:2561852/150‑1 (MQ=255)
          tgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCACCAGGCGCAgggt                                                                                                                                             <  2:4996781/150‑3 (MQ=255)
           gcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGAcc                                                                                                                                            >  2:6017340/1‑150 (MQ=255)
           gcGGCCAACCGTGCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACACACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTAGTGCGACACAAGCCCCTGCACCAGGCGCAGGGTGCAggggc                                                                                                                                            <  2:7584899/150‑4 (MQ=255)
            cGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCt                                                                                                                                           <  1:3102615/150‑1 (MQ=255)
             ggCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCTCCTGCACCAGGCGCAGGGTGCAggggctt                                                                                                                                          <  2:8353339/150‑6 (MQ=255)
              gCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGa                                                                                                                                         <  2:6681057/150‑1 (MQ=255)
              gCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGa                                                                                                                                         <  2:5219221/150‑1 (MQ=255)
              gCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGa                                                                                                                                         >  2:2156690/1‑150 (MQ=255)
               ccAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAg                                                                                                                                        >  1:3535836/1‑150 (MQ=255)
                cAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGc                                                                                                                                       <  1:1155863/150‑1 (MQ=255)
                 aaCCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCg                                                                                                                                      >  2:2382543/1‑150 (MQ=255)
                    cGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGaca                                                                                                                                   <  1:6535703/150‑1 (MQ=255)
                    cGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGaca                                                                                                                                   >  1:6070904/1‑150 (MQ=255)
                          ccTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGc                                                                                                                             >  2:1018218/1‑150 (MQ=255)
                          ccTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGc                                                                                                                             <  1:2508117/150‑1 (MQ=255)
                           cTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCt                                                                                                                            >  2:5027984/1‑150 (MQ=255)
                           cTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCt                                                                                                                            >  1:557768/1‑150 (MQ=255)
                           cTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCt                                                                                                                            >  1:6004685/1‑150 (MQ=255)
                            tCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACACGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTg                                                                                                                           <  2:1863046/150‑1 (MQ=255)
                            tCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTg                                                                                                                           <  1:855603/150‑1 (MQ=255)
                                 gggCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCt                                                                                                                      <  1:5159382/150‑1 (MQ=255)
                                     tACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTAc                                                                                                                  >  2:4131065/1‑150 (MQ=255)
                                        cccGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGAc                                                                                                               >  1:3355248/1‑150 (MQ=255)
                                        cccGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGAc                                                                                                               <  1:6413465/150‑1 (MQ=255)
                                        cccGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGAc                                                                                                               >  1:6914265/1‑150 (MQ=255)
                                         ccGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGTGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGAcc                                                                                                              >  1:1678153/1‑150 (MQ=255)
                                                 gCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTgcgc                                                                                                      >  2:3574489/1‑150 (MQ=255)
                                                 gCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTgcgc                                                                                                      <  1:5314431/150‑1 (MQ=255)
                                                  ccGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCt                                                                                                     <  2:8255886/150‑1 (MQ=255)
                                                   cGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCtt                                                                                                    >  2:5744002/1‑150 (MQ=255)
                                                       cacaAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATc                                                                                                <  1:1466729/150‑1 (MQ=255)
                                                        acaAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATcc                                                                                               <  1:6275058/150‑1 (MQ=255)
                                                         caAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCg                                                                                              >  2:1492681/1‑150 (MQ=255)
                                                            aCGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcac                                                                                           >  2:3292322/1‑150 (MQ=255)
                                                            aCGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcac                                                                                           >  1:1017632/1‑150 (MQ=255)
                                                               ccAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcaccca                                                                                        >  1:470730/1‑150 (MQ=255)
                                                               ccAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcaccca                                                                                        <  1:4938109/150‑1 (MQ=255)
                                                                cAGGAGCTGATCACCGCCCTGCTGCTCGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcacccac                                                                                       <  2:7707442/150‑1 (MQ=255)
                                                                cAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcacccac                                                                                       <  1:5744002/150‑1 (MQ=255)
                                                                cAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcacccac                                                                                       <  1:4043747/150‑1 (MQ=255)
                                                                  ggAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCt                                                                                     <  2:1791825/150‑1 (MQ=255)
                                                                     gCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCt                                                                                  <  1:3778051/150‑1 (MQ=255)
                                                                       tGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTcg                                                                                >  1:8392054/1‑150 (MQ=255)
                                                                       tGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTcg                                                                                >  1:6162176/1‑150 (MQ=255)
                                                                        gATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTcga                                                                               >  2:6956449/1‑150 (MQ=255)
                                                                          tCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTcgacc                                                                             >  2:5575349/1‑150 (MQ=255)
                                                                            aCCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTcgaccga                                                                           >  1:67976/1‑150 (MQ=255)
                                                                                cccTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTgg                                                                       >  1:293957/1‑150 (MQ=255)
                                                                                 ccTGCTGCTCGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGa                                                                      <  1:7512611/150‑1 (MQ=255)
                                                                                 ccTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGa                                                                      <  1:6648792/150‑1 (MQ=255)
                                                                                 ccTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGa                                                                      <  1:1218759/150‑1 (MQ=255)
                                                                                  ctgctgCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAg                                                                     <  2:4522849/150‑1 (MQ=255)
                                                                                   tgctgcACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGt                                                                    <  2:3884471/150‑1 (MQ=255)
                                                                                   tgctgcACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGt                                                                    >  2:8709201/1‑150 (MQ=255)
                                                                                    gctgcACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGtt                                                                   >  1:5481961/1‑150 (MQ=255)
                                                                                      tgcACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCa                                                                 <  2:2802914/150‑1 (MQ=255)
                                                                                       gcACGCCAGCCGCGGCGGCCTCGACCCTATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTTGAGTTCAc                                                                <  1:2244825/150‑1 (MQ=255)
                                                                                        cACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCAcc                                                               >  2:3162315/1‑150 (MQ=255)
                                                                                          cGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCAcccc                                                             >  1:4998194/1‑150 (MQ=255)
                                                                                           gCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCAccccc                                                            <  1:7036390/150‑1 (MQ=255)
                                                                                           gCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCAccccc                                                            <  2:6492288/150‑1 (MQ=255)
                                                                                           gCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCAccccc                                                            >  2:6217413/1‑150 (MQ=255)
                                                                                                ccGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGa                                                       <  1:4589232/150‑1 (MQ=255)
                                                                                                  gcggcgGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACg                                                     >  1:2014044/1‑150 (MQ=255)
                                                                                                       ggcCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCg                                                <  2:7676495/150‑1 (MQ=255)
                                                                                                       ggcCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCg                                                <  2:8559264/150‑1 (MQ=255)
                                                                                                           tCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAg                                            >  2:545253/1‑150 (MQ=255)
                                                                                                             gACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCt                                          >  1:4360200/1‑150 (MQ=255)
                                                                                                                ccGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCtgtg                                       >  1:8063058/1‑150 (MQ=255)
                                                                                                                 cGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGc                                      <  2:3726869/150‑1 (MQ=255)
                                                                                                                  gATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCa                                     <  1:2773584/150‑1 (MQ=255)
                                                                                                                  gATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCa                                     >  2:2076331/1‑150 (MQ=255)
                                                                                                                  gATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCA‑CCCCCAGGACGAACCGGTAGCTGTGCAc                                    <  1:3835772/150‑1 (MQ=255)
                                                                                                                     cTATTGCGACACCAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGt                                  <  1:925779/150‑1 (MQ=255)
                                                                                                                        ttGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGAc                               <  2:7080792/150‑1 (MQ=255)
                                                                                                                        ttGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGAc                               <  1:2658963/150‑1 (MQ=255)
                                                                                                                               acaAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTgcagc                        >  2:1371842/1‑150 (MQ=255)
                                                                                                                                tcaggtccTGCACCAGGCGCAGGGTGCAGGGGCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTgcagca                       >  2:4904221/7‑150 (MQ=255)
                                                                                                                                tcaggcccTGCACCCTGCGCCTGGTGCAGGACCTGGGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTgcagca                       >  2:8000743/6‑150 (MQ=255)
                                                                                                                                caAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTgcagca                       >  1:5849962/1‑150 (MQ=255)
                                                                                                                                 aaGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcac                      >  2:4785214/1‑150 (MQ=255)
                                                                                                                                  aGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcacc                     <  2:3838553/150‑1 (MQ=255)
                                                                                                                                      ccTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTCGCTGTGCACGTGACCTGcagcacccagc                 >  1:6353483/1‑150 (MQ=255)
                                                                                                                                      ccTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcacccagc                 >  2:2898336/1‑150 (MQ=255)
                                                                                                                                       cTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcacccagca                >  2:6884443/1‑150 (MQ=255)
                                                                                                                                       cTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcacccagca                >  2:6810369/1‑150 (MQ=255)
                                                                                                                                           aCCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTg            >  2:1961525/1‑150 (MQ=255)
                                                                                                                                             ccTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTggg          >  2:719349/1‑150 (MQ=255)
                                                                                                                                             ccTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTggg          >  2:1224583/1‑150 (MQ=255)
                                                                                                                                                   ccTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGGGCGAAAg    >  1:2801316/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGCCTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGGGCGAAAGCC  >  NC_002947/5393731‑5394029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑