Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,395,428 T→G 6.5% intergenic (+520/‑202) PP_4738 → / → PP_mr56 hypothetical protein/C4 ncRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,395,4280TG 6.5% 1266.6 / 51.3 410intergenic (+520/‑202)PP_4738/PP_mr56hypothetical protein/C4 ncRNA
Reads supporting (aligned to +/- strand):  ref base T (197/183);  new base G (15/13);  total (214/196)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 415 total aligned reads displayed.

TTACACAACTCATCCAAAGCACCCTGCCCGTCGGGC‑GGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGT  >  NC_002947/5395279‑5395577
                                                                                                                                                      |                                                                                                                                                     
ttACACAACTCATCCAAAGCACCCTGCCCGTCGGGC‑GGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCt                                                                                                                                                       >  2:6262910/1‑150 (MQ=255)
   cacaACTCATCCAAAGCACCCTGCCCGTCGGGC‑GGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCgg                                                                                                                                                    <  1:1246736/150‑1 (MQ=255)
   cacaACTCATCCAAAGCACCCTGCCCGTCGGGC‑GGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCgg                                                                                                                                                    <  2:2795248/150‑1 (MQ=255)
    acaACTCATCCAAAGCACCCTGCCCGTCGGGC‑GGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCACATCCAAAGAAGAAGACGCCCTCggg                                                                                                                                                   >  2:158498/1‑150 (MQ=255)
      aaCTCATCCAAAGCACCCTGCCCGTCGGGC‑GGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCg                                                                                                                                                 >  2:2086633/1‑150 (MQ=255)
           aTCCAAAGCACCCTGCGCGTCGTGC‑GTCTGCTGATTAAATCAGTAGACACGGCTAAGCATGTTGTTCCGAGAGCGTAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCTAATCCATAGAATAAGACTCGCTCGTTCGTCttt                                                                                                                                            <  2:5572243/150‑1 (MQ=255)
            tCCAAAGCACCCTGCCCGTCGGGC‑GGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCtttt                                                                                                                                           >  1:6532064/1‑150 (MQ=255)
            tCCAAAGCACCCTGCCCGTCGGGC‑GGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCtttt                                                                                                                                           <  1:681077/150‑1 (MQ=255)
               aaaGCACCCTGCCCGTCGGGC‑GGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCTGACGGGTGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCttttttt                                                                                                                                        <  2:3495433/150‑1 (MQ=255)
               aaaGCACCCTGCCCGTCGGGC‑GGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCttttttt                                                                                                                                        >  2:1076034/1‑150 (MQ=255)
               aaaGCACCCTGCCCGTCGGGC‑GGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCttttttt                                                                                                                                        <  2:8330638/150‑1 (MQ=255)
               aaaGCACCCTGCCCGTCGGGC‑GGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCttttttt                                                                                                                                        <  1:5802076/150‑1 (MQ=255)
               aaaGCACCCTGCCCGTCGGGC‑GGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCttttttt                                                                                                                                        <  1:7939807/150‑1 (MQ=255)
                caGCACCCTGCCCGTCGGGC‑GGCTGCGGATTAAATCAGTAGACCCGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAAT‑CCCCCGGCTCCACCAAATCCAAAGAAAAAGACGCCCGAGGGCGTcttcttcttt                                                                                                                                      <  2:7971498/149‑5 (MQ=255)
                 aGCACCCGGCCCGTCGGGC‑GGCTGCGGATTAAATCCGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAAAAGACGCCCGAGGGCGTcttcttcttt                                                                                                                                      <  2:2200706/150‑5 (MQ=255)
                  gCACCCTGCCCGTCGGGC‑GGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTtgtg                                                                                                                                     >  1:3802729/1‑150 (MQ=255)
                  gCACCCTGCCCGTCGGGC‑GGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCACGGGCGGCTGTTTTtgtg                                                                                                                                     >  1:1642991/1‑150 (MQ=255)
                  cgcagccgCCCGACGGGCAGGGTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTtgt                                                                                                                                      >  1:5239546/8‑150 (MQ=255)
                      ccTGCCCGTCGGGC‑GGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTg                                                                                                                                 >  2:1815212/1‑150 (MQ=255)
                      ccTGCCCGTCGGGC‑GGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTg                                                                                                                                 <  1:1030965/150‑1 (MQ=255)
                      ccTGCCCGTCGGGC‑GGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTg                                                                                                                                 <  2:6112497/150‑1 (MQ=255)
                      ccTGCCCGTCGGGC‑GGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTg                                                                                                                                 >  2:6436928/1‑150 (MQ=255)
                            cGTCGGGC‑GGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAAttt                                                                                                                           <  2:2022633/150‑1 (MQ=255)
                                 ggc‑ggcTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGgcg                                                                                                                      <  2:5369051/150‑1 (MQ=255)
                                     ggcTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTg                                                                                                                   <  2:7669514/150‑1 (MQ=255)
                                        tGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCgg                                                                                                                >  2:6059452/1‑150 (MQ=255)
                                           ggATTACATCAGGAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGCCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTc                                                                                                             <  2:2669126/150‑1 (MQ=255)
                                                  aTCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAAt                                                                                                      >  1:5488853/1‑150 (MQ=255)
                                                  aTCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAAt                                                                                                      >  1:6470491/1‑150 (MQ=255)
                                                   tCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATc                                                                                                     <  1:4444384/150‑1 (MQ=255)
                                                      gTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGa                                                                                                  <  2:1669598/150‑1 (MQ=255)
                                                      gTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGa                                                                                                  >  2:3016682/1‑150 (MQ=255)
                                                      gTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGa                                                                                                  <  2:725300/150‑1 (MQ=255)
                                                           cacGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTaa                                                                                             <  1:1174414/150‑1 (MQ=255)
                                                            acGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTagc                                                                                            >  1:2793734/1‑148 (MQ=255)
                                                            acGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAc                                                                                            >  1:8607729/1‑150 (MQ=255)
                                                            acGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAAATTGGGCGCTGCGGGTCGAAAAATAGGATTTAAc                                                                                            >  1:4852161/1‑150 (MQ=255)
                                                              ggCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAccc                                                                                          <  2:3770508/150‑1 (MQ=255)
                                                                   aGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTc                                                                                     <  1:7179195/150‑1 (MQ=255)
                                                                   aGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCACTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTc                                                                                     <  2:3597227/150‑1 (MQ=255)
                                                                     cATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTTTGGCTGGAATTTTGGCGCTGCGGGTCGAAAAATAGGATTTAAacccaatatt                                                                                   >  1:1466410/1‑144 (MQ=255)
                                                                     cATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCtt                                                                                   >  1:6268812/1‑150 (MQ=255)
                                                                      aTGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGGCTGGAATTTGGGCGCTGCGGGTCGAAAAATAGGAGTTAACCCCATTCTTg                                                                                  >  1:7406742/1‑150 (MQ=255)
                                                                       tGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTTTGCCGGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCGATCTTGt                                                                                 >  1:4005165/1‑150 (MQ=255)
                                                                        gtagtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCgg                                                                                                   >  2:4787949/1‑131 (MQ=255)
                                                                         tagtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAg                                                                               <  2:4262559/150‑1 (MQ=255)
                                                                          agtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAgg                                                                              <  1:8251253/150‑1 (MQ=255)
                                                                               cGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATAGGATTTAACCCCTTTCTTGTAGGGCGtt                                                                         >  1:4502819/1‑150 (MQ=255)
                                                                                gACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGttt                                                                        <  1:5612252/150‑1 (MQ=255)
                                                                                 aCAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                       <  2:7114626/150‑1 (MQ=255)
                                                                                 aCAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                       <  2:6567990/150‑1 (MQ=255)
                                                                                     cGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCTGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCaa                                                                   >  2:3141678/1‑150 (MQ=255)
                                                                                          tACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATa                                                              <  2:2898750/150‑1 (MQ=255)
                                                                                             tGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATActg                                                           >  2:8105054/1‑150 (MQ=255)
                                                                                             tGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATActg                                                           <  2:1416221/150‑1 (MQ=255)
                                                                                             tGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATActg                                                           >  2:6971878/1‑150 (MQ=255)
                                                                                             tGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGAGCGTTTCCCAAAGATActg                                                           >  2:2561159/1‑150 (MQ=255)
                                                                                              ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATActgc                                                          <  1:2393119/150‑1 (MQ=255)
                                                                                               gCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATActgcc                                                         >  1:6417369/1‑150 (MQ=255)
                                                                                                     gggggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTTTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATAATGCCTGCGcc                                                   >  1:2579248/1‑150 (MQ=255)
                                                                                                      ggggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCt                                                  >  2:5077389/1‑150 (MQ=255)
                                                                                                          ttCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAAACGGATTTAAACCCTTTATTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGatt                                              >  1:4275830/1‑150 (MQ=255)
                                                                                                             aaaTCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCGTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTcc                                           <  1:5963757/150‑1 (MQ=255)
                                                                                                                 ccccccGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTg                                       >  2:8195676/1‑150 (MQ=255)
                                                                                                                 ccccccGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTg                                       <  2:1257540/150‑1 (MQ=255)
                                                                                                                  cccccGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGc                                      <  1:2086633/150‑1 (MQ=255)
                                                                                                                          tCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACAGCCTGCGCCTGTTTTCCGTTGCGGTCAatt                              >  1:6926139/1‑150 (MQ=255)
                                                                                                                           ccaccaAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAtttt                             <  1:2924734/150‑1 (MQ=255)
                                                                                                                             accaAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAtttttt                           <  2:3443907/150‑1 (MQ=255)
                                                                                                                              ccaAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTTTGCCTGGAATTTGGGCGCGGCGGGTCGAAAAATCGGATTTAAACCCATTCTTGTAGGGCGTTTACCAAATATACTGCCTGCGCCTGTTTTGCGTTGCGGTCAtttttta                          >  1:8204230/1‑149 (MQ=255)
                                                                                                                              ccaAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTc                          >  2:6720609/1‑150 (MQ=255)
                                                                                                                               caAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCa                         <  1:6704528/150‑1 (MQ=255)
                                                                                                                               caAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCa                         <  2:5239546/150‑1 (MQ=255)
                                                                                                                               caAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCa                         <  1:3686253/150‑1 (MQ=255)
                                                                                                                                aaaTCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAg                        >  2:328758/1‑150 (MQ=255)
                                                                                                                                   tCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTTTGCCTGGAATTTTGGCGCTGCGGGTCGAAAAATAGGATTTAACCCCTTTATTGTATGGCGTTTGCCAAAGATAATGCCTGCGCCTGTTTTACGTTGCGGTCATTTTTTAAGGGt                     >  1:3110690/1‑150 (MQ=255)
                                                                                                                                      aaaGAAGAAGACGCCCTCGGGCGTCTTTTTTTTTTCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCATTAATGTATGGCGTTTACCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTAAGGGtttt                  >  1:5888071/1‑150 (MQ=255)
                                                                                                                                      aaaGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGtttt                  >  2:453195/1‑150 (MQ=255)
                                                                                                                                      aaaGAAGAAGACGCCCTCGGGCGTCATTTTTTGTGCCTGGAATTTTGGCGCTTCGGGTCGAAAAAAAGGATTTAAACCCATTATTGTAGGGCGTTTACCAAAGAAACTGCCTGCGCCTGTTTTCCGGTGCGGTCATTTTTTCAGGGtttt                  >  1:8447509/1‑150 (MQ=255)
                                                                                                                                       aagaagaagACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTa                 <  1:3545899/150‑1 (MQ=255)
                                                                                                                                       aagaagaagACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTa                 <  2:5734752/150‑1 (MQ=255)
                                                                                                                                       aagaagaagACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTa                 <  2:5961623/150‑1 (MQ=255)
                                                                                                                                       aagaagaagACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTa                 <  1:8098095/150‑1 (MQ=255)
                                                                                                                                       aagaagaagACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTa                 >  2:3962186/1‑150 (MQ=255)
                                                                                                                                       aagaagaagACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATAGGATTTAACCCCTTTCTTGTAGGGCGGTTCCCAAAGATACATCCTGCGCCTGTTTTACGTTGCGGTCATTTTTTAATGGTTTTa                 >  1:4630196/1‑150 (MQ=255)
                                                                                                                                        agaagaagaCGCCCTCGGGCGTCTTTTTTTGTTCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAACCCTTTATTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTAAGGGTTTTAg                >  1:1568156/1‑150 (MQ=255)
                                                                                                                                          aaaaagaCGCCCGAGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGt                                                                                 <  2:8775436/80‑1 (MQ=39)
                                                                                                                                          aaaaagaCGCCCGAGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGt                                                                                 >  1:8775436/4‑83 (MQ=39)
                                                                                                                                          aaaaagaCCCCCGAGGGCGTCTTTATTTGAGCCTGGAATTTGGGCGCTGCGGGTCGAAAAAACGGAATTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGAATACCGTTCCGGTCATTTTATCAGGGTTTTAGtc              >  1:5147531/4‑150 (MQ=255)
                                                                                                                                           agaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtct             <  2:8744669/150‑1 (MQ=255)
                                                                                                                                           aaaagaCGCCCGAGGGCGTCTTCTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtct             >  2:7899389/3‑150 (MQ=255)
                                                                                                                                            gaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtctc            <  1:3366963/150‑1 (MQ=255)
                                                                                                                                            gaagaCGCCCTCGGGCGTCATTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtctc            >  1:7577778/1‑150 (MQ=255)
                                                                                                                                              agaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCgg          <  2:8607729/150‑1 (MQ=255)
                                                                                                                                               gaCGCCCTCGGGCGTCTTTTTTTGTGCCAGGAATTTGGGCGCTGCGGGTCGAAAAATCCGATTTAACCCCTATCTTGTAGGGCGATTCCCAAAGAAACAGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGt         >  1:1312780/1‑150 (MQ=255)
                                                                                                                                                 cGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTgg       <  2:2301228/150‑1 (MQ=255)
                                                                                                                                                 cGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTgg       <  2:8446701/150‑1 (MQ=255)
                                                                                                                                                  gCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTggg      >  1:3236153/1‑150 (MQ=255)
                                                                                                                                                    ccTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACAGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTc    >  1:224539/1‑150 (MQ=255)
                                                                                                                                                    ccTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAACCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTc    >  1:4539713/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TTACACAACTCATCCAAAGCACCCTGCCCGTCGGGC‑GGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGT  >  NC_002947/5395279‑5395577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑