BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,423,816	T→A	8.5%	V84V (GTA→GTT) ‡	PP_4758 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,423,816	0	T	A	8.5%	1343.9 / 82.2	421	V84V (GTA→GTT) ‡	PP_4758	MFS transporter
Reads supporting (aligned to +/- strand): ref base T (232/153); new base A (21/15); total (253/168)
Fisher's exact test for biased strand distribution p-value = 8.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01

Only 100 of 421 total aligned reads displayed.

TTCGGCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACA  >  NC_002947/5423669‑5423964

ttCGGCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTAc                                                                                                                                                    >  2:1789766/1‑150 (MQ=255)

   ggCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTc                                                                                                                                                 <  1:3759889/150‑1 (MQ=255)

    gCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCg                                                                                                                                                >  2:3307418/1‑150 (MQ=255)

      cACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGTACCTTCGaa                                                                                                                                              <  1:6226167/150‑1 (MQ=255)

      cACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGaa                                                                                                                                              <  1:4745716/150‑1 (MQ=255)

       aCCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGTACCTTCGAAc                                                                                                                                             <  2:1406139/150‑1 (MQ=255)

        ccccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGTACCTTCGAACa                                                                                                                                            <  2:7585804/150‑1 (MQ=255)

         cccGAGCAGAACACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGTACCTTCGAACat                                                                                                                                           <  2:6963307/150‑2 (MQ=255)

         cccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAg                                                                                                                                           >  1:5854599/1‑150 (MQ=255)

          ccGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAgc                                                                                                                                          <  2:8462681/150‑1 (MQ=255)

           cGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAgcg                                                                                                                                         >  2:6338297/1‑150 (MQ=255)

            gAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAgcgc                                                                                                                                        <  2:1205074/150‑1 (MQ=255)

            gAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAgcgc                                                                                                                                        >  1:811568/1‑150 (MQ=255)

                aGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTaa                                                                                                                                    >  2:5941878/1‑150 (MQ=255)

                 gAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAAc                                                                                                                                   >  2:3191549/1‑150 (MQ=255)

                    aaCGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGa                                                                                                                                <  1:766985/150‑1 (MQ=255)

                        cagcaCGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATgaa                                                                                                                            <  2:721968/150‑1 (MQ=255)

                         agcaCGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATgaag                                                                                                                           >  2:869312/1‑150 (MQ=255)

                                      gggTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCAc                                                                                                              >  1:7077525/1‑150 (MQ=255)

                                       ggTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCAcc                                                                                                             >  1:6703998/1‑150 (MQ=255)

                                         tttCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCATAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGa                                                                                                           >  2:8263900/1‑150 (MQ=255)

                                         tttCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGa                                                                                                           >  1:4603636/1‑150 (MQ=255)

                                         tttCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGa                                                                                                           >  1:2086651/1‑150 (MQ=255)

                                          ttCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGaa                                                                                                          >  2:2305822/1‑150 (MQ=255)

                                            cGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGc                                                                                                        >  1:3358337/1‑150 (MQ=255)

                                            cGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGc                                                                                                        >  1:1507736/1‑150 (MQ=255)

                                              ttCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCa                                                                                                      >  1:1096168/1‑150 (MQ=255)

                                                cTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATa                                                                                                    <  1:83719/150‑1 (MQ=255)

                                                cTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATa                                                                                                    <  2:1572109/150‑1 (MQ=255)

                                                 tGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAg                                                                                                   <  2:4574376/150‑1 (MQ=255)

                                                 tGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAg                                                                                                   >  1:5181142/1‑150 (MQ=255)

                                                 tGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAg                                                                                                   <  2:5099331/150‑1 (MQ=255)

                                                 tGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAg                                                                                                   >  1:2872691/1‑150 (MQ=255)

                                                 tGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAg                                                                                                   <  2:7517909/150‑1 (MQ=255)

                                                   cacaAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGc                                                                                                 >  1:1400603/1‑150 (MQ=255)

                                                    acaAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGcc                                                                                                >  1:7872724/1‑150 (MQ=255)

                                                      aaaGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGc                                                                                              >  1:6338964/1‑150 (MQ=255)

                                                        aGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAg                                                                                            >  1:2053860/1‑150 (MQ=255)

                                                        aGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGAAg                                                                                            <  1:8741488/150‑1 (MQ=255)

                                                            cATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCg                                                                                                                  <  1:1305022/124‑1 (MQ=255)

                                                            cATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCg                                                                                                                  >  2:1305022/1‑124 (MQ=255)

                                                            cATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCt                                                                                        <  1:5568269/150‑1 (MQ=255)

                                                            cATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCt                                                                                        <  2:1046725/150‑1 (MQ=255)

                                                            cATGGCCGTGGCCACCAGCCCCCAGGTGAACATGAGACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCt                                                                                        <  2:4768288/150‑1 (MQ=255)

                                                                  cGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGcc                                                                                  >  2:7082652/1‑150 (MQ=255)

                                                                  cGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGcc                                                                                  >  1:4248531/1‑150 (MQ=255)

                                                                  cGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGcc                                                                                  >  2:3770683/1‑150 (MQ=255)

                                                                    tGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCa                                                                                >  1:2566483/1‑150 (MQ=255)

                                                                        caccaGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTc                                                                            >  2:6671348/1‑150 (MQ=255)

                                                                           caGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCt                                                                         >  1:1148625/1‑150 (MQ=255)

                                                                               ccccAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTcc                                                                     >  2:5492541/1‑150 (MQ=255)

                                                                                 ccAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAg                                                                   >  1:2400416/1‑150 (MQ=255)

                                                                                 ccAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAg                                                                   >  1:5081327/1‑150 (MQ=255)

                                                                                  cAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAgg                                                                  >  1:8665936/1‑150 (MQ=255)

                                                                                     gTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTgc                                                               >  1:6858859/1‑150 (MQ=255)

                                                                                       gAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTgcgg                                                             >  1:5996405/1‑150 (MQ=255)

                                                                                       gAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTgcgg                                                             >  2:2671046/1‑150 (MQ=255)

                                                                                        aaCATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTgcggg                                                            >  1:349899/1‑150 (MQ=255)

                                                                                           aTGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTgcgggcgg                                                         >  1:1768172/1‑150 (MQ=255)

                                                                                                  gggTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCGGCGCTGATGCCCAGGTCGCTGTCCAGGTGCGGGCGGACGAAGc                                                  >  2:4173444/1‑150 (MQ=255)

                                                                                                  gggTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGc                                                  <  1:4489649/150‑1 (MQ=255)

                                                                                                    gTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCa                                                >  2:3535707/1‑150 (MQ=255)

                                                                                                     tCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCaa                                               >  2:1470678/1‑150 (MQ=255)

                                                                                                       aGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATg                                             >  2:4338997/1‑150 (MQ=255)

                                                                                                        gCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGt                                            >  1:5497239/1‑150 (MQ=255)

                                                                                                         ccACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGtt                                           >  1:3563146/1‑150 (MQ=255)

                                                                                                          cacaGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTg                                          >  2:3680524/1‑150 (MQ=255)

                                                                                                          cacaGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTg                                          <  2:2898343/150‑1 (MQ=255)

                                                                                                                 gggCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGt                                   >  2:4840026/1‑150 (MQ=255)

                                                                                                                 gggCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGt                                   >  1:8625853/1‑150 (MQ=255)

                                                                                                                  ggCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTc                                  >  1:7801567/1‑150 (MQ=255)

                                                                                                                     aCCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAg                               >  2:5348601/1‑150 (MQ=255)

                                                                                                                      ccGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAgg                              >  1:5945117/1‑150 (MQ=255)

                                                                                                                      ccGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAgg                              <  2:6621430/150‑1 (MQ=255)

                                                                                                                       cGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGt                                                                             <  1:3249587/102‑1 (MQ=255)

                                                                                                                         aCCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAg                           >  1:226402/1‑150 (MQ=255)

                                                                                                                         aCCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAg                           <  1:4945674/150‑1 (MQ=255)

                                                                                                                           cTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGtt                         <  2:1148450/150‑1 (MQ=255)

                                                                                                                           cTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGtt                         <  1:2996443/150‑1 (MQ=255)

                                                                                                                               tGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACg                     <  2:678745/150‑1 (MQ=255)

                                                                                                                                gcagcagcaTGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGa                    <  2:2083150/150‑1 (MQ=255)

                                                                                                                                 cagcagcaTGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGAt                   >  2:3077053/1‑150 (MQ=255)

                                                                                                                                 cagcagcaTGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGAt                   <  1:6410286/150‑1 (MQ=255)

                                                                                                                                   gcagcaTGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGa                 <  2:7851711/150‑1 (MQ=255)

                                                                                                                                       gctGTTCGAAGTACCTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACat             >  1:7322527/3‑150 (MQ=255)

                                                                                                                                       gctGTTCGAAGTACCTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACat             >  2:4106854/3‑150 (MQ=255)

                                                                                                                                       caTGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACat             <  1:2673210/150‑1 (MQ=255)

                                                                                                                                        aTGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatg            >  2:1385296/1‑150 (MQ=255)

                                                                                                                                         tGTTCGAAGTACCTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatga           >  1:1897171/1‑150 (MQ=255)

                                                                                                                                         tGTTCGAAGTACCTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatga           >  1:8500409/1‑150 (MQ=255)

                                                                                                                                         tGTTCGAAGTACCTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatga           >  1:5036622/1‑150 (MQ=255)

                                                                                                                                         tGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatga           <  1:8282325/150‑1 (MQ=255)

                                                                                                                                           ttCGAAGTACCTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatgatg         >  2:6495949/1‑150 (MQ=255)

                                                                                                                                           ttCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACatgatg         >  1:7527471/1‑150 (MQ=255)

                                                                                                                                           ttCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGCCGAGGTAGTTGACGATGAACatgatg         >  1:613842/1‑150 (MQ=255)

                                                                                                                                              gAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACg      >  1:7157753/1‑150 (MQ=255)

                                                                                                                                              gAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACg      >  2:8084970/1‑150 (MQ=255)

                                                                                                                                              gAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACg      >  2:2581436/1‑150 (MQ=255)

                                                                                                                                                aGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGaa    >  1:5353174/1‑150 (MQ=255)

                                                                                                                                                aGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGaa    <  2:3974523/150‑1 (MQ=255)

TTCGGCCACCCCGAGCAGAAAACGCAGCACGTAGAACTGGGTTTCGTTCTGCACAAAGGCCATGGCCGTGGCCACCAGCCCCCAGGTGAACATGATACGGGTCAGCCACAGCCGGGCACCGACCTTTTGCAGCAGCATGTTCGAAGGTACTTCGAACAGCGCGTAACCGATGAAGAACAGCCCGGCACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACA  >  NC_002947/5423669‑5423964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`