Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,435,021 T→C 5.3% S99G (AGC→GGC)  PP_4769 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,435,0210TC 5.3% 1145.9 / 31.6 355S99G (AGC→GGC) PP_4769hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (162/172);  new base C (10/9);  total (173/182)
Fisher's exact test for biased strand distribution p-value = 8.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 355 total aligned reads displayed.

TAGCGGGTGGCAGCACGGATCAGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAACTGCTGTTGTTTGCTGTCGCCAC  >  NC_002947/5434873‑5435167
                                                                                                                                                    |                                                                                                                                                  
 aGCGGGTGGCAGCACGGATCAGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTgg                                                                                                                                                  >  1:1027065/1‑150 (MQ=255)
 aGCGGGTGGCAGCACGGATCAGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTgg                                                                                                                                                  <  1:6109066/150‑1 (MQ=255)
    gggTGGCAGCACGGATCAGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCcg                                                                                                                                               >  2:7706124/1‑150 (MQ=255)
     ggTGGCAGCACGGATCAGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCcgc                                                                                                                                              <  1:1393240/150‑1 (MQ=255)
         gcagcaCGGATCAGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCg                                                                                                                                          <  2:6238239/150‑1 (MQ=255)
          cagcaCGGATCAGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGa                                                                                                                                         <  2:2329092/150‑1 (MQ=255)
           agcaCGGATCAGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACGCGGCCAGCCGGGTCGac                                                                                                                                        <  1:3283702/150‑2 (MQ=255)
           agcaCGGATCAGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGAt                                                                                                                                        >  1:5481725/1‑150 (MQ=255)
            gcaCGGATCAGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATc                                                                                                                                       >  1:4887869/1‑150 (MQ=255)
             caCGGATCAGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATca                                                                                                                                      >  2:5963497/1‑150 (MQ=255)
              aCGGATCAGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCCAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATcac                                                                                                                                     <  1:7613718/150‑1 (MQ=255)
              aCGGATCAGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATcac                                                                                                                                     >  2:331795/1‑150 (MQ=255)
              aCGGATCAGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATcac                                                                                                                                     <  1:7344906/150‑1 (MQ=255)
              aCGGATCAGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATcac                                                                                                                                     >  1:2175825/1‑150 (MQ=255)
                  aTCAGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATcc                                                                                                                                 <  2:533590/150‑1 (MQ=255)
                    cAGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAg                                                                                                                               <  2:6817224/150‑1 (MQ=255)
                    cAGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAg                                                                                                                               >  1:8299770/1‑150 (MQ=255)
                     aGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAgg                                                                                                                              <  2:1905558/150‑1 (MQ=255)
                     aGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAgg                                                                                                                              <  1:3773364/150‑1 (MQ=255)
                     aGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAgg                                                                                                                              <  2:322968/150‑1 (MQ=255)
                       tGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGt                                                                                                                            >  1:4962665/1‑150 (MQ=255)
                          cAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTCAt                                                                                                                         >  1:8295804/1‑150 (MQ=255)
                          cAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAAt                                                                                                                         >  2:6905244/1‑150 (MQ=255)
                            aCGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGc                                                                                                                       <  1:1904037/150‑1 (MQ=255)
                            aCGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGc                                                                                                                       <  2:435954/150‑1 (MQ=255)
                             cGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGcc                                                                                                                      <  2:5538714/150‑1 (MQ=255)
                             cGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGcc                                                                                                                      <  2:4668068/150‑1 (MQ=255)
                                      ccccGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCa                                                                                                             <  1:4834403/150‑1 (MQ=255)
                                      ccccGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCa                                                                                                             >  1:5968113/1‑150 (MQ=255)
                                        ccGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGt                                                                                                           >  2:7811979/1‑150 (MQ=255)
                                         cGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTc                                                                                                          >  1:7158185/1‑150 (MQ=255)
                                           gCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCAc                                                                                                        >  2:6713515/1‑150 (MQ=255)
                                             aCGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCt                                                                                                      >  2:7284044/1‑150 (MQ=255)
                                                ccATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTg                                                                                                   >  2:4795728/1‑150 (MQ=255)
                                                ccATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTg                                                                                                   <  1:1506826/150‑1 (MQ=255)
                                                ccATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTg                                                                                                   <  1:2496657/150‑1 (MQ=255)
                                                ccATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTg                                                                                                   <  1:4182201/150‑1 (MQ=255)
                                                  aTACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGc                                                                                                 <  1:789321/150‑1 (MQ=255)
                                                     caccacCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGa                                                                                              <  1:6139401/150‑1 (MQ=255)
                                                     caccacCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGa                                                                                              >  2:156311/1‑150 (MQ=255)
                                                      accaccGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAg                                                                                             >  1:8796702/1‑150 (MQ=255)
                                                        caccGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGcc                                                                                           >  1:8086503/1‑150 (MQ=255)
                                                        caccGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGcc                                                                                           >  2:4612041/1‑150 (MQ=255)
                                                          ccGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCAt                                                                                         <  1:3036926/150‑1 (MQ=255)
                                                              ttCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACt                                                                                     <  1:8428541/150‑1 (MQ=255)
                                                               tCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTg                                                                                    >  2:2974961/1‑150 (MQ=255)
                                                                  gTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTgcgc                                                                                 >  2:5143570/1‑150 (MQ=255)
                                                                    gCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTg                                                                               <  1:3576003/150‑1 (MQ=255)
                                                                     ccATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTgg                                                                              >  2:1718265/1‑150 (MQ=255)
                                                                      cATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGa                                                                             <  1:8011466/150‑1 (MQ=255)
                                                                       aTGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGAt                                                                            <  2:2774171/150‑1 (MQ=255)
                                                                       aTGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGAt                                                                            >  2:2003429/1‑150 (MQ=255)
                                                                         ggCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGa                                                                          >  2:8013154/1‑150 (MQ=255)
                                                                              tGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGt                                                                     >  1:3829599/1‑150 (MQ=255)
                                                                              cgggatACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACCGCGCTGGATGAACGGt                                                                     <  1:7760780/145‑1 (MQ=255)
                                                                               ggATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGtt                                                                    <  1:4250652/150‑1 (MQ=255)
                                                                               ggATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGtt                                                                    <  1:6499333/150‑1 (MQ=255)
                                                                                gATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGttt                                                                   <  1:69100/150‑1 (MQ=255)
                                                                                  ttACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGa                                                                 >  1:4518477/1‑150 (MQ=255)
                                                                                  ttACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGa                                                                 >  1:8598545/1‑150 (MQ=255)
                                                                                  ttACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGa                                                                 <  1:4064531/150‑1 (MQ=255)
                                                                                  ttACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGa                                                                 >  2:4230202/1‑150 (MQ=255)
                                                                                   tACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAg                                                                <  1:1180643/150‑1 (MQ=255)
                                                                                   tACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAg                                                                <  1:637751/150‑1 (MQ=255)
                                                                                    aCCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAgg                                                               <  1:198953/150‑1 (MQ=255)
                                                                                     cccGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGgc                                                              >  2:8173137/1‑150 (MQ=255)
                                                                                      ccGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGgcg                                                             <  1:6039569/150‑1 (MQ=255)
                                                                                           ccAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCt                                                        <  2:5638095/150‑1 (MQ=255)
                                                                                            cagcagCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTc                                                       <  2:4887869/150‑1 (MQ=255)
                                                                                            cagcagCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTc                                                       >  2:5893200/1‑150 (MQ=255)
                                                                                              gcagcaAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGa                                                     <  2:4042218/150‑1 (MQ=255)
                                                                                                   aaGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCg                                                >  1:5095649/1‑150 (MQ=255)
                                                                                                    aGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGATACCGa                                               <  2:1068664/150‑1 (MQ=255)
                                                                                                    aGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGa                                               <  2:7611891/150‑1 (MQ=255)
                                                                                                     gTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAg                                              >  2:8105455/1‑150 (MQ=255)
                                                                                                     gTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAg                                              <  2:6990854/150‑1 (MQ=255)
                                                                                                     gTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAg                                              <  1:2885487/150‑1 (MQ=255)
                                                                                                      tCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAgg                                             <  2:8562364/150‑1 (MQ=255)
                                                                                                        gAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGtt                                           >  1:525973/1‑150 (MQ=255)
                                                                                                         aaaTCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTg                                          >  1:1124283/1‑150 (MQ=255)
                                                                                                          aaTCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTgc                                         <  1:348731/150‑1 (MQ=255)
                                                                                                           aTCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTgcg                                        >  1:926356/1‑150 (MQ=255)
                                                                                                                      cGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATctgct                             >  2:6428328/1‑150 (MQ=255)
                                                                                                                       ggAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATctgctg                            <  2:8329250/150‑1 (MQ=255)
                                                                                                                       ggAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATctgctg                            <  1:7655203/150‑1 (MQ=255)
                                                                                                                         aGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGa                          <  2:7336634/150‑1 (MQ=255)
                                                                                                                          ggCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGaa                         <  1:2681580/150‑1 (MQ=255)
                                                                                                                             cAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAActg                      >  1:1628159/1‑150 (MQ=255)
                                                                                                                              aGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCGGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAActgc                     <  2:2501211/150‑1 (MQ=255)
                                                                                                                               gCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAActgct                    <  1:1792372/150‑1 (MQ=255)
                                                                                                                                  ccaccTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAACTGCtgtt                 <  2:4282462/150‑1 (MQ=255)
                                                                                                                                     ccTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAACTGCTgttgtt              <  2:3613927/150‑1 (MQ=255)
                                                                                                                                     ccTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAACTGCTgttgtt              <  2:8299762/150‑1 (MQ=255)
                                                                                                                                        ggTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAACTGCTGTTGTTTGc           <  1:8334576/150‑1 (MQ=255)
                                                                                                                                         gTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAACTGCTGTTGTTTGCt          <  1:6807355/150‑1 (MQ=255)
                                                                                                                                         atCGACGCGGCCAGCCGGGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAACTGCTGTTGTTTGCt          >  1:8578275/2‑150 (MQ=255)
                                                                                                                                          tCGACGCGGCCAGCCGGGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAACTGCTGTTGTTTGCTg         >  2:2925400/1‑150 (MQ=255)
                                                                                                                                          tCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAACTGCTGTTGTTTGCTg         <  2:1436623/150‑1 (MQ=255)
                                                                                                                                           cGACGCGGCCAGCCGGGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAACTGCTGTTGTTTGCTGt        >  1:3165552/1‑150 (MQ=255)
                                                                                                                                               ccGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAACTGCTGTTGTTTGCTGTCGcc    >  1:412059/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TAGCGGGTGGCAGCACGGATCAGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCACCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAACTGCTGTTGTTTGCTGTCGCCAC  >  NC_002947/5434873‑5435167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑