Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,563,372 C→A 5.2% R294L (CGT→CTT)  purA ← adenylosuccinate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,563,3720CA 5.2% 1154.0 / 34.8 340R294L (CGT→CTT) purAadenylosuccinate synthetase
Reads supporting (aligned to +/- strand):  ref base C (145/176);  new base A (8/10);  total (154/186)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 340 total aligned reads displayed.

GTTGATGGTTTCCAGGCCGTCCAGTACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGCCGACGCCGGAACCGGTGGAAATACCACCGGCAGT  >  NC_002947/5563224‑5563520
                                                                                                                                                    |                                                                                                                                                    
 ttGATGGTTTCCAGGCCGTCCAGTACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGc                                                                                                                                                    >  2:4885817/1‑150 (MQ=255)
   gATGGTTTCCAGGCCGTCCAGTACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCtt                                                                                                                                                  <  2:2445267/150‑1 (MQ=255)
    aTGGTTTCCAGGCCGTCCAGTACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTg                                                                                                                                                 <  1:5198368/150‑1 (MQ=255)
     tGGTTTCCAGGCCGTCCAGTACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTgg                                                                                                                                                <  2:5159182/150‑1 (MQ=255)
       gTTTCCAGGCCGTCCAGTACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGcc                                                                                                                                              >  2:6755/1‑150 (MQ=255)
        tttCCAGGCCGTCCAGTACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCa                                                                                                                                             >  1:3296846/1‑150 (MQ=255)
         ttCCAGGCCGTCCAGTACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCac                                                                                                                                            >  2:1786982/1‑149 (MQ=255)
         ttCCAGGCCGTCCAGTACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCAAGCGTGGCCac                                                                                                                                            <  1:1976619/150‑2 (MQ=255)
         ttCCAGGCCGTCCAGTACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCAAGCGTGGCCac                                                                                                                                            <  1:5360252/150‑2 (MQ=255)
          tCCAGGCCGTCCAGTACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAg                                                                                                                                           <  1:4837789/150‑1 (MQ=255)
          tCCAGGCCGTCCAGTACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAg                                                                                                                                           >  2:1054660/1‑150 (MQ=255)
           ccAGGCCGTCCAGTACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCTTGGCCAAGCGTGGCCacga                                                                                                                                          <  1:6419457/150‑4 (MQ=255)
           ccAGGCCGTCCAGTACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAgg                                                                                                                                          <  1:7417590/150‑1 (MQ=255)
           ccAGGCCGTCCAGTACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAgg                                                                                                                                          <  1:5388716/150‑1 (MQ=255)
           ccAGGCCGTCCAGTACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAgg                                                                                                                                          <  2:6418134/150‑1 (MQ=255)
           ccAGGCCGTCCAGTACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAgg                                                                                                                                          <  2:7918849/150‑1 (MQ=255)
            cAGGCCGTCCAGTACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGt                                                                                                                                         >  1:4159562/1‑150 (MQ=255)
                  gTCCAGTACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCAcc                                                                                                                                   <  2:8581809/150‑1 (MQ=255)
                    ccAGTACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCgg                                                                                                                                 >  1:5595968/1‑150 (MQ=255)
                     cAGTACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGt                                                                                                                                <  2:4980200/150‑1 (MQ=255)
                      aGTACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGtc                                                                                                                               <  2:5378247/150‑1 (MQ=255)
                      aGTACGTCCAGCGGGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCCTCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGtc                                                                                                                               <  2:2783426/150‑1 (MQ=255)
                        tACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGtctc                                                                                                                             <  1:2675259/150‑1 (MQ=255)
                           gTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCtcgtc                                                                                                                          >  2:5097840/1‑150 (MQ=255)
                              cAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGaa                                                                                                                       >  2:3247193/1‑150 (MQ=255)
                                gCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACa                                                                                                                     <  1:2349464/150‑1 (MQ=255)
                                gCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACa                                                                                                                     >  2:7594988/1‑150 (MQ=255)
                                  ttGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACAAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGt                                                                                                                   <  2:7735026/150‑1 (MQ=255)
                                     gTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTtcg                                                                                                                <  2:2510031/150‑1 (MQ=255)
                                      tCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTtcgg                                                                                                               <  1:7735293/150‑1 (MQ=255)
                                        aGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTtcggtc                                                                                                             >  2:2675444/1‑150 (MQ=255)
                                         ggCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTtcggtcg                                                                                                            <  1:4432960/150‑1 (MQ=255)
                                         ggCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTtcggtcg                                                                                                            <  1:3696601/150‑1 (MQ=255)
                                           cAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCggg                                                                                                          <  1:8800520/150‑1 (MQ=255)
                                            aGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGa                                                                                                         <  2:1698364/150‑1 (MQ=255)
                                                 cccGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGa                                                                                                    <  2:3832458/150‑1 (MQ=255)
                                                 cccGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGa                                                                                                    <  2:1576570/150‑1 (MQ=255)
                                                  ccGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGACCGGAc                                                                                                   >  2:5613687/1‑150 (MQ=255)
                                                  ccGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGAc                                                                                                   <  1:1078412/150‑1 (MQ=255)
                                                  ccGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGAc                                                                                                   >  1:6155037/1‑150 (MQ=255)
                                                   cGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGAcc                                                                                                  >  1:3043028/1‑150 (MQ=255)
                                                   cGAGATGATGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGAcc                                                                                                  >  2:4664818/1‑150 (MQ=255)
                                                     agaTGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCgg                                                                                                <  1:2729377/150‑1 (MQ=255)
                                                      gaTGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGa                                                                                               >  2:2011050/1‑150 (MQ=255)
                                                       aTGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGac                                                                                              >  1:5420573/1‑149 (MQ=255)
                                                        tGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAAc                                                                                             <  1:1752251/150‑1 (MQ=255)
                                                            gTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGACCGGACCGGAACCCAc                                                                                         >  2:7263425/1‑150 (MQ=255)
                                                                   tCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAgtg                                                                                  <  2:8501567/150‑1 (MQ=255)
                                                                        ggCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTa                                                                             <  2:3465132/150‑1 (MQ=255)
                                                                        ggCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTa                                                                             <  2:6103510/150‑1 (MQ=255)
                                                                        ggCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTa                                                                             <  1:1868892/150‑1 (MQ=255)
                                                                             gACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTtcggtcg                                                                                                            >  2:762978/1‑114 (MQ=255)
                                                                               cGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTg                                                                      <  2:4368362/150‑1 (MQ=255)
                                                                               cGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTg                                                                      <  1:1250983/150‑1 (MQ=255)
                                                                                 cAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGt                                                                    <  2:8601147/150‑1 (MQ=255)
                                                                                 cAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGt                                                                    <  2:5919824/150‑1 (MQ=255)
                                                                                     aTCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATa                                                                <  1:8135345/150‑1 (MQ=255)
                                                                                      tCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATAc                                                               >  2:6306181/1‑150 (MQ=255)
                                                                                         cGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGa                                                            <  2:2332210/150‑1 (MQ=255)
                                                                                         cGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGa                                                            <  1:5949248/150‑1 (MQ=255)
                                                                                         cGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGa                                                            <  2:5859588/150‑1 (MQ=255)
                                                                                         cGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGa                                                            <  2:7441016/150‑1 (MQ=255)
                                                                                         cGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGa                                                            <  2:3071563/150‑1 (MQ=255)
                                                                                          ggCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAg                                                           >  2:3969773/1‑150 (MQ=255)
                                                                                          ggCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAg                                                           <  1:5546516/150‑1 (MQ=255)
                                                                                          ggCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAg                                                           <  1:6799348/150‑1 (MQ=255)
                                                                                           gCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAgg                                                          <  2:7886449/150‑1 (MQ=255)
                                                                                             aTCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGAt                                                        >  1:6344507/1‑150 (MQ=255)
                                                                                                gAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAggcctac                                                     <  2:8712428/150‑6 (MQ=255)
                                                                                                gAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTa                                                     <  1:8215717/150‑1 (MQ=255)
                                                                                                gAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCGTGGCCAAGGTAGCACCGGTCTCGTCGAACAGGTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGtc                                                     >  2:5797571/1‑149 (MQ=255)
                                                                                                        cGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCg                                                 >  1:2169737/1‑146 (MQ=255)
                                                                                                        cGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCg                                                 <  2:2169737/146‑1 (MQ=255)
                                                                                                        cGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGt                                             <  2:4242884/150‑1 (MQ=255)
                                                                                                          cAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAg                                                           >  1:3398880/1‑134 (MQ=255)
                                                                                                                 gggCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTCGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTCGTCGAGGTACATCGGGc                                    >  1:5004271/1‑150 (MQ=255)
                                                                                                                 gggCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGc                                    <  2:1349495/150‑1 (MQ=255)
                                                                                                                 gggCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGc                                    <  2:8274785/150‑1 (MQ=255)
                                                                                                                 gggCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGc                                    <  2:5582458/150‑1 (MQ=255)
                                                                                                                 gggCACGGCCGGTGGTGGAACCGAACTCGTGGCCACACTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGc                                    <  1:1451389/150‑1 (MQ=255)
                                                                                                                  ggCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGcc                                   <  1:4748457/150‑1 (MQ=255)
                                                                                                                   gCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGCCg                                  >  2:4796052/1‑150 (MQ=255)
                                                                                                                     aCGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGCCGAc                                >  2:5690912/1‑150 (MQ=255)
                                                                                                                       ggCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGCCGACGc                              <  1:6514682/150‑1 (MQ=255)
                                                                                                                        gCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGCCGACGcc                             <  2:599724/150‑1 (MQ=255)
                                                                                                                                tggAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGCCGACGCCGGAACCgg                     >  1:8525255/1‑150 (MQ=255)
                                                                                                                                      cGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAgg                                              <  1:4951524/119‑1 (MQ=255)
                                                                                                                                      cGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGCCGACGCCGGAACCGGTGGaaa               >  1:7094021/1‑150 (MQ=255)
                                                                                                                                      cGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGCCGACGCCGGAACCGGTGGaaa               >  2:3024118/1‑150 (MQ=255)
                                                                                                                                      cGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGCCGACGCCGGAACCGGTGGaaa               >  1:8388207/1‑150 (MQ=255)
                                                                                                                                       gAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGCCGACGCCGGAACCGGTGGAAAt              >  2:4308141/1‑150 (MQ=255)
                                                                                                                                        aaCTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAAGGCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGTGGTACATCGGGCCGAGGGCGGTACCGGTGGAATTa             >  2:7185752/1‑150 (MQ=255)
                                                                                                                                             gTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGCCGACGCCGGAACCGGTGGAAATaccacc        >  2:695281/1‑150 (MQ=255)
                                                                                                                                             gTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGCCGACGCCGGAACCGGTGGAAATaccacc        >  1:3467541/1‑150 (MQ=255)
                                                                                                                                             gTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGCCGACGCCGGAACCGGTGGAAATaccacc        >  1:5755801/1‑150 (MQ=255)
                                                                                                                                               ggCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGCCGACGCCGGAACCGGTGGAAATACCACCgg      <  1:1587925/150‑1 (MQ=255)
                                                                                                                                                gCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGCCGACGCCGGAACCGGTGGAAATACCACCGGc     >  1:7992411/1‑150 (MQ=255)
                                                                                                                                                 ccACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGCCGACGCCGGAACCGGTGGAAATACCACCGGCa    <  1:3212801/150‑1 (MQ=255)
                                                                                                                                                 ccACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGCCGACGCCGGAACCGGTGGAAATACCACCGGCa    >  1:2082337/1‑150 (MQ=255)
                                                                                                                                                   aCGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGCCGACGCCGGAACCGGTGGAAATACCACCGGCAgt  <  2:3370844/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTTGATGGTTTCCAGGCCGTCCAGTACGTCCAGCTTGGTCAGGCAGATGCCCGAGATGCTGTTGACGTCGATGGCGCGACGCAGGATCACGGCATCGAACCAGCCGCAACGACGGGCACGGCCGGTGGTGGAACCGAACTCGTGGCCACGCTTGGCCAAGGTAGCACCGGTCTCGTCGAACAGTTCGGTCGGGAACGGACCGGAACCCACGCGAGTGGTGTAGGCCTTGGTGATACCGAGGATGTAGTCGAGGTACATCGGGCCGACGCCGGAACCGGTGGAAATACCACCGGCAGT  >  NC_002947/5563224‑5563520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑