BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,617,017	C→G	6.6%	R337R (CGC→CGG) ‡	PP_4931 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,617,017	0	C	G	6.6%	1226.2 / 55.0	365	R337R (CGC→CGG) ‡	PP_4931	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (165/176); new base G (13/11); total (178/187)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

Only 100 of 385 total aligned reads displayed.

ATTGCCGCAGCGCAGAAGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCGCTGGGCGTACCGGCCTGGGA  >  NC_002947/5616868‑5617166

aTTGCCGCAGCGCAGAAGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAgcgc                                                                                                                                                       >  2:2637013/1‑150 (MQ=255)

    ccGCAGCGCAGAAGGAAATCGACACCCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCctgc                                                                                                                                                   <  2:1967081/150‑1 (MQ=255)

     cgcagcgcagAAGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCctgct                                                                                                                                                  >  2:7850502/1‑150 (MQ=255)

      gcagcgcagAAGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCctgctg                                                                                                                                                 <  1:712886/150‑1 (MQ=255)

         gcgcagAAGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCtggtg                                                                                                                                              <  2:7396836/150‑1 (MQ=255)

          cgcagAAGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCtggtgg                                                                                                                                             <  2:6091217/150‑1 (MQ=255)

          cgcagAAGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCCACCAGCAGGCGCTGCtggtcg                                                                                                                                             <  1:6086474/150‑3 (MQ=255)

          cgcagAAGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCCACCAGCAGGCGCTGCtggtcg                                                                                                                                             <  2:2756835/150‑3 (MQ=255)

           gcagAAGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTggg                                                                                                                                            >  1:2479788/1‑150 (MQ=255)

            cagAAGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGGCGCTGCTGGTCGGc                                                                                                                                           <  1:1856516/150‑1 (MQ=255)

            cagAAGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGGCGCTGCTGGTCGGc                                                                                                                                           <  1:4529087/150‑1 (MQ=255)

               aaGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTgg                                                                                                                                        <  1:3844185/150‑1 (MQ=255)

                aGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGc                                                                                                                                       <  2:7527999/150‑1 (MQ=255)

                 ggAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGcc                                                                                                                                      >  2:6898711/1‑150 (MQ=255)

                   aaaTCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGcc                                                                                                                                      >  1:5556296/1‑148 (MQ=255)

                    aaTCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGAc                                                                                                                                   >  1:3212328/1‑150 (MQ=255)

                    aaTCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGAc                                                                                                                                   >  1:6802686/1‑150 (MQ=255)

                    aaTCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGAc                                                                                                                                   >  2:7902167/1‑150 (MQ=255)

                    aaTCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCAGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGAc                                                                                                                                   >  1:2731064/1‑150 (MQ=255)

                     aTCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGAcc                                                                                                                                  <  1:2943040/150‑1 (MQ=255)

                        gACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCaaa                                                                                                                               >  2:8414379/1‑150 (MQ=255)

                        gACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCaaa                                                                                                                               <  1:3674654/150‑1 (MQ=255)

                          cacccTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAact                                                                                                                             <  2:3248066/147‑1 (MQ=255)

                           aaCCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAactg                                                                                                                            >  1:4221345/1‑150 (MQ=255)

                           aaCCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAactg                                                                                                                            >  2:6205501/1‑150 (MQ=255)

                           aaCCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAactg                                                                                                                            <  1:1751104/150‑1 (MQ=255)

                           aaCCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAactg                                                                                                                            <  2:8039765/150‑1 (MQ=255)

                            aCCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAactgg                                                                                                                           <  2:3502107/150‑1 (MQ=255)

                            aCCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAactgg                                                                                                                           <  1:6594888/150‑1 (MQ=255)

                              ctgctgCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACTCTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGTTGGTGGGCTGGCCGACCAAactggca                                                                                                                         <  2:530386/150‑1 (MQ=255)

                                 ctgcCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACGCTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAactggcactg                                                                                                                      >  2:1418009/1‑150 (MQ=255)

                                    ccATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAActggcactggca                                                                                                                   >  2:4656898/1‑150 (MQ=255)

                                     cATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACtggcactggcac                                                                                                                  >  2:929326/1‑150 (MQ=255)

                                       tGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCg                                                                                                                <  2:1024835/150‑1 (MQ=255)

                                       tGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCg                                                                                                                <  2:7030107/150‑1 (MQ=255)

                                         ggTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGa                                                                                                              <  2:8714656/150‑1 (MQ=255)

                                           tCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACt                                                                                                            >  2:2583127/1‑150 (MQ=255)

                                            cGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACtt                                                                                                           <  1:2874364/150‑1 (MQ=255)

                                              ctCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCa                                                                                                         <  2:5644185/149‑1 (MQ=255)

                                               tCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAg                                                                                                        >  1:4895767/1‑150 (MQ=255)

                                               tCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAg                                                                                                        >  1:3255061/1‑150 (MQ=255)

                                                 agagCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCg                                                                                                      >  2:5436370/1‑150 (MQ=255)

                                                      cTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCgg                                                                                                 <  1:6219657/150‑1 (MQ=255)

                                                      cTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCgg                                                                                                 <  2:432364/150‑1 (MQ=255)

                                                      cTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCgg                                                                                                 <  2:814786/150‑1 (MQ=255)

                                                       tGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCggg                                                                                                >  1:77256/1‑150 (MQ=255)

                                                             gcTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCa                                                                                          <  2:6571861/150‑1 (MQ=255)

                                                             gcTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCa                                                                                          <  1:2227787/150‑1 (MQ=255)

                                                             gcTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCa                                                                                          <  2:3723715/150‑1 (MQ=255)

                                                               tGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGc                                                                                        >  2:7722883/1‑150 (MQ=255)

                                                                    cacaCTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTACTCAGCCACCt                                                                                   >  2:5221047/1‑150 (MQ=255)

                                                                    cacaCTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCt                                                                                   <  2:3954857/150‑1 (MQ=255)

                                                                          gcgcGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACGGGACTTCAGCGACCGGGTAATCAGCCACCTGGAgcg                                                                             <  1:4063564/150‑1 (MQ=255)

                                                                          gcgcGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAgcg                                                                             >  1:7957110/1‑150 (MQ=255)

                                                                          gcgcGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAgcg                                                                             >  1:4699550/1‑150 (MQ=255)

                                                                          gcgcGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAgcg                                                                             >  2:4528205/1‑150 (MQ=255)

                                                                             cgTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGa                                                                          >  1:2307745/1‑150 (MQ=255)

                                                                                cGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACgg                                                                       <  2:2456499/150‑1 (MQ=255)

                                                                                   ccGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCAt                                                                    >  1:1010755/1‑150 (MQ=255)

                                                                                   ccGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCAt                                                                    >  2:4178670/1‑150 (MQ=255)

                                                                                   ccGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCAt                                                                    >  2:2489603/1‑150 (MQ=255)

                                                                                         tGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTcc                                                              >  1:1433433/1‑150 (MQ=255)

                                                                                         tGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCGTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTcc                                                              <  2:6037618/150‑1 (MQ=255)

                                                                                          gAGCCTGCACAAGCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACGGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCca                                                             <  2:3122322/150‑1 (MQ=255)

                                                                                            gCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCcaca                                                           >  1:8421141/1‑150 (MQ=255)

                                                                                             ccTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACaa                                                          <  2:4378956/150‑1 (MQ=255)

                                                                                             ccTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACaa                                                          >  2:5030696/1‑150 (MQ=255)

                                                                                               tGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGc                                                        >  1:2641966/1‑150 (MQ=255)

                                                                                                 cacaATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGcac                                                      >  1:7626861/1‑150 (MQ=255)

                                                                                                  acaATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGcaca                                                     >  2:2823347/1‑150 (MQ=255)

                                                                                                  acaATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGcaca                                                     >  1:8181692/1‑150 (MQ=255)

                                                                                                      tCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGcc                                                 >  2:4429207/1‑150 (MQ=255)

                                                                                                        cGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGa                                               >  1:8768453/1‑150 (MQ=255)

                                                                                                         ggCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGAc                                              <  2:7354081/150‑1 (MQ=255)

                                                                                                             tGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTga                                          <  1:718230/150‑1 (MQ=255)

                                                                                                             tGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTga                                          >  2:6470468/1‑150 (MQ=255)

                                                                                                             tGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACGGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTga                                          <  1:377215/150‑1 (MQ=255)

                                                                                                                 aCGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTgaccga                                      >  1:3360862/1‑150 (MQ=255)

                                                                                                                 aCGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTgaccga                                      >  1:7528733/1‑150 (MQ=255)

                                                                                                                  cGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCTCAGGCCGACCTgaccgac                                     <  2:2947923/150‑1 (MQ=255)

                                                                                                                  cGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTgaccgac                                     <  1:5471466/150‑1 (MQ=255)

                                                                                                                  cGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTgaccgac                                     <  1:1986698/150‑1 (MQ=255)

                                                                                                                      cGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGc                                 >  2:1285024/1‑150 (MQ=255)

                                                                                                                           aaCGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGc                            >  2:8599772/1‑150 (MQ=255)

                                                                                                                              gCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCt                         >  1:348065/1‑150 (MQ=255)

                                                                                                                                 ttccttGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCg                      >  1:8667443/1‑150 (MQ=255)

                                                                                                                                    cttGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACg                                                                        >  1:5861737/1‑97 (MQ=255)

                                                                                                                                    cttGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCGCTg                   >  1:4145481/1‑150 (MQ=255)

                                                                                                                                     ttGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCGCTgg                  <  1:4338126/150‑1 (MQ=255)

                                                                                                                                       gCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCGCTgg                  <  2:8267832/148‑1 (MQ=255)

                                                                                                                                       gCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCGCTGGGc                <  1:8330065/150‑1 (MQ=255)

                                                                                                                                        cccaCCAGCAGGCGCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACTTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCGCTGGGCg               >  1:5167972/4‑150 (MQ=255)

                                                                                                                                        cccaCCAGCAGGCGCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCGCTGGGCg               >  2:5929641/4‑150 (MQ=255)

                                                                                                                                         ccaCCAGCAGGCGCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCGCTGGGCGt              >  2:1711045/3‑150 (MQ=255)

                                                                                                                                          caCCAGCAGGCGCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATACGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCGCTGGGCGTa             >  1:112017/2‑150 (MQ=255)

                                                                                                                                            ccAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCGCTgg                  <  1:1537012/143‑1 (MQ=255)

                                                                                                                                               gcagcGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCGCTGGGCGTACCGGc        >  2:4044500/1‑150 (MQ=255)

                                                                                                                                                  gcgcCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCGCTGGGCGTACCGGCCTg     <  1:1339218/150‑1 (MQ=255)

                                                                                                                                                     ccTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCGCTGGGCGTACCGGCCTGGGa  <  2:7617813/150‑1 (MQ=255)

                                                                                                                                                     ccTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCGCTGGGCGTACCGGCCTGGGa  <  2:4244961/150‑1 (MQ=255)

ATTGCCGCAGCGCAGAAGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCGCTGGGCGTACCGGCCTGGGA  >  NC_002947/5616868‑5617166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`