Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,711,125 C→G 6.2% H46Q (CAC→CAG) ‡ PP_5009 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,711,1250CG 6.2% 1032.7 / 41.9 306H46Q (CAC→CAG) ‡PP_5009hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (166/121);  new base G (10/9);  total (176/130)
Fisher's exact test for biased strand distribution p-value = 8.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 311 total aligned reads displayed.

GACATCGACGCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGCGCCGCAGA  >  NC_002947/5710976‑5711274
                                                                                                                                                     |                                                                                                                                                     
   aTCGACGCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAGGCGT‑GCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCa                                                                                                                                                   >  2:3371666/1‑150 (MQ=255)
   aTCGACGCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAACGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGc                                                                                                                                                    <  2:2280772/150‑1 (MQ=255)
    tCGACGCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCa                                                                                                                                                   >  2:5119456/1‑150 (MQ=255)
    tCGACGCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCa                                                                                                                                                   >  1:2455509/1‑150 (MQ=255)
         gCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCGGTGCAaccat                                                                                                                                              <  1:120119/150‑2 (MQ=255)
            aTGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCGGTGCAaccatgga                                                                                                                                           <  2:5814265/150‑5 (MQ=255)
             tGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCtc                                                                                                                                          <  1:7555780/150‑1 (MQ=255)
                ctgctgTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCtcaca                                                                                                                                       <  2:7331855/150‑1 (MQ=255)
                 tgctgTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCtcacat                                                                                                                                      >  1:4677388/1‑150 (MQ=255)
                 tgctgTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCtcacat                                                                                                                                      >  1:3693104/1‑150 (MQ=255)
                 tgctgTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCtcacat                                                                                                                                      >  2:5412968/1‑150 (MQ=255)
                  gctgTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCtcacatc                                                                                                                                     <  2:7000527/150‑1 (MQ=255)
                     gTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCtcacatcaca                                                                                                                                  >  2:6151587/1‑150 (MQ=255)
                        gCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCg                                                                                                                               >  2:1794804/1‑150 (MQ=255)
                           aaCCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTc                                                                                                                            >  2:3866402/1‑150 (MQ=255)
                              ccacccaGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGAt                                                                                                                         >  2:4485696/1‑150 (MQ=255)
                               cacccaGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATc                                                                                                                        <  2:4364020/150‑1 (MQ=255)
                                 cccaGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCt                                                                                                                      >  2:2419617/1‑150 (MQ=255)
                                    aGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTc                                                                                                                   <  1:6487606/150‑1 (MQ=255)
                                    aGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTc                                                                                                                   <  2:6936245/150‑1 (MQ=255)
                                        tGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCa                                                                                                               >  1:8493848/1‑150 (MQ=255)
                                         gACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCAt                                                                                                              >  2:2098/1‑150 (MQ=255)
                                           cGTTCACGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATcc                                                                                                            <  1:7372187/150‑1 (MQ=255)
                                           cGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATcc                                                                                                            <  2:8210648/150‑1 (MQ=255)
                                            gTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCt                                                                                                           >  1:3478001/1‑150 (MQ=255)
                                            gTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCt                                                                                                           >  2:5689348/1‑150 (MQ=255)
                                            gTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCt                                                                                                           <  2:1525091/150‑1 (MQ=255)
                                             ttCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTg                                                                                                          >  1:2066766/1‑150 (MQ=255)
                                             ttCAAGCAAAGCGTGGCAGACTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCAGCCTg                                                                                                          >  2:5282416/1‑150 (MQ=255)
                                              tctaGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGc                                                                                                         >  1:2564207/4‑150 (MQ=255)
                                              tCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGc                                                                                                         >  1:3594840/1‑150 (MQ=255)
                                                   cAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGaa                                                                                                    >  2:6044821/1‑150 (MQ=255)
                                                    aaaGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAAc                                                                                                   >  1:3139657/1‑150 (MQ=255)
                                                     aaGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAAcc                                                                                                  >  2:7976781/1‑150 (MQ=255)
                                                     aaGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAAcc                                                                                                  <  1:7962981/150‑1 (MQ=255)
                                                      aGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCGGTGCAaccat                                                                                                                                              <  1:78550/105‑2 (MQ=255)
                                                                ccTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAg                                                                                       <  1:4925034/150‑1 (MQ=255)
                                                                        tcCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGAtctc                                                                               >  1:8443921/1‑150 (MQ=255)
                                                                          cAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTccc                                                                             >  2:6701676/1‑150 (MQ=255)
                                                                          cAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTccc                                                                             >  2:2458537/1‑150 (MQ=255)
                                                                           aaaCGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTccc                                                                             <  2:2708543/149‑1 (MQ=255)
                                                                           aaaCGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTccc                                                                             >  1:2708543/1‑149 (MQ=255)
                                                                           aaaCGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCa                                                                            <  2:4788895/150‑1 (MQ=255)
                                                                           aaaCGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCa                                                                            >  1:4061977/1‑150 (MQ=255)
                                                                           aaaCGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCa                                                                            >  1:3948468/1‑150 (MQ=255)
                                                                           aaaCGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATACATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCa                                                                            >  1:5609936/1‑150 (MQ=255)
                                                                            aaCGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAg                                                                           <  1:7755148/150‑1 (MQ=255)
                                                                             aCGACCCAGCCTGAGGGACGTATTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAgg                                                                          <  1:173099/150‑1 (MQ=255)
                                                                                  ccAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCaga                                                                     <  2:6490119/150‑1 (MQ=255)
                                                                                  ccAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCaga                                                                     >  1:6070473/1‑150 (MQ=255)
                                                                                  ccAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCaga                                                                     <  2:1076035/150‑1 (MQ=255)
                                                                                  ccAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAACACAGGTGATCTCCCAGGTCaga                                                                     <  1:1867558/150‑1 (MQ=255)
                                                                                     gCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCg                                                                  <  2:7714089/150‑1 (MQ=255)
                                                                                                gTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGAc                                                       <  1:1608039/150‑1 (MQ=255)
                                                                                                  cTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGa                                                     >  1:4829220/1‑150 (MQ=255)
                                                                                                   ttATCAACAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAg                                                    <  2:8397827/150‑1 (MQ=255)
                                                                                                   ttATCAAAATAAGCATTTCAGGTTTTCAGCGACCTGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAg                                                    <  1:1908736/150‑1 (MQ=255)
                                                                                                   ttATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAg                                                    <  2:8662944/150‑1 (MQ=255)
                                                                                                   ttATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAg                                                    <  2:7214770/150‑1 (MQ=255)
                                                                                                    tATCAACAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGc                                                   <  1:6704816/150‑1 (MQ=255)
                                                                                                     aTCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCt                                                  >  1:8681105/1‑150 (MQ=255)
                                                                                                      tCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCtt                                                 >  1:4230706/1‑150 (MQ=255)
                                                                                                       cAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCCCATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTtc                                                <  1:4309524/150‑1 (MQ=255)
                                                                                                       cAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTtc                                                >  2:7850030/1‑150 (MQ=255)
                                                                                                         aaaGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTtctc                                              <  1:4485696/150‑1 (MQ=255)
                                                                                                         aaaGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTtctc                                              <  2:3255091/150‑1 (MQ=255)
                                                                                                          aagaagCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCa                                             <  1:1390780/150‑1 (MQ=255)
                                                                                                          aagaagCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCa                                             <  1:5814453/150‑1 (MQ=255)
                                                                                                          aagaagCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCa                                             <  2:3233903/150‑1 (MQ=255)
                                                                                                          aagaagCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCa                                             <  2:2066475/150‑1 (MQ=255)
                                                                                                            gaagCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAgg                                           >  2:2832798/1‑150 (MQ=255)
                                                                                                                cATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCAtt                                       <  1:2421712/150‑1 (MQ=255)
                                                                                                                     cAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTg                                  <  1:7053988/150‑1 (MQ=255)
                                                                                                                      aGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTgg                                 >  1:7694819/1‑150 (MQ=255)
                                                                                                                        gTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGcc                               >  2:2935542/1‑150 (MQ=255)
                                                                                                                          tttCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCaa                             >  2:7120301/1‑150 (MQ=255)
                                                                                                                          tttCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCaa                             <  1:3308016/150‑1 (MQ=255)
                                                                                                                           ttCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAg                            <  2:1467682/150‑1 (MQ=255)
                                                                                                                                 gACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACgt                      <  1:8077864/150‑1 (MQ=255)
                                                                                                                                 gACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACgt                      >  1:2267185/1‑150 (MQ=255)
                                                                                                                                     ggTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGc                  >  2:6447272/1‑150 (MQ=255)
                                                                                                                                       tATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCaa                <  2:1547751/150‑1 (MQ=255)
                                                                                                                                       tATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCaa                >  2:4180307/1‑150 (MQ=255)
                                                                                                                                         tCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACt              >  2:3685914/1‑150 (MQ=255)
                                                                                                                                           ggtGGTTGCGGTGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTaa            >  1:5454904/3‑150 (MQ=255)
                                                                                                                                           cATGGTTGCACCGCAACCCCCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCCTGTTCTGGCCCAGGAACGGGGGCACCtcc            >  2:3726591/1‑148 (MQ=255)
                                                                                                                                           cATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTaa            <  1:6203441/150‑1 (MQ=255)
                                                                                                                                           cATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTaa            >  1:8214142/1‑150 (MQ=255)
                                                                                                                                            gtGGTTGCGGTGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAg           >  1:2966570/2‑150 (MQ=255)
                                                                                                                                            gtGGTTGCGGTGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAg           >  1:293017/2‑150 (MQ=255)
                                                                                                                                            aTGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAg           >  2:6613565/1‑150 (MQ=255)
                                                                                                                                            aTGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAg           <  1:5119456/150‑1 (MQ=255)
                                                                                                                                            aTGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAg           >  1:4195711/1‑150 (MQ=255)
                                                                                                                                            aTGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAg           >  2:7443334/1‑150 (MQ=255)
                                                                                                                                              ggTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAgcg         <  1:8776169/150‑1 (MQ=255)
                                                                                                                                                 tGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGcgccg      <  2:8110981/150‑1 (MQ=255)
                                                                                                                                                   cACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGCGCCGCa    <  1:5381089/150‑1 (MQ=255)
                                                                                                                                                    aCCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTACATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGCGCCGCAg   >  2:8642437/1‑150 (MQ=255)
                                                                                                                                                     ccGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGAc                                                       <  1:1488226/97‑1 (MQ=255)
                                                                                                                                                     ccGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGCGCCGCAGa  >  2:464601/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GACATCGACGCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGCGCCGCAGA  >  NC_002947/5710976‑5711274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑