Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,711,126 C→T 6.2% R47C (CGC→TGC)  PP_5009 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,711,1260CT 6.2% 962.4 / 33.9 306R47C (CGC→TGC) PP_5009hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (166/121);  new base T (10/9);  total (176/130)
Fisher's exact test for biased strand distribution p-value = 8.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 310 total aligned reads displayed.

ACATCGACGCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGCGCCGCAGA  >  NC_002947/5710977‑5711274
                                                                                                                                                     |                                                                                                                                                    
  aTCGACGCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAGGCGT‑GCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCa                                                                                                                                                   >  2:3371666/1‑150 (MQ=255)
   tCGACGCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCa                                                                                                                                                   >  1:2455509/1‑150 (MQ=255)
   tCGACGCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCa                                                                                                                                                   >  2:5119456/1‑150 (MQ=255)
        gCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCGGTGCAaccat                                                                                                                                              <  1:120119/150‑2 (MQ=255)
           aTGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCGGTGCAaccatgga                                                                                                                                           <  2:5814265/150‑5 (MQ=255)
           aTGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCGGTGCAaccatgga                                                                                                                                           <  1:8301477/150‑5 (MQ=255)
            tGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCtc                                                                                                                                          <  1:7555780/150‑1 (MQ=255)
                tgctgTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCtcacat                                                                                                                                      >  1:4677388/1‑150 (MQ=255)
                tgctgTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCtcacat                                                                                                                                      >  2:5412968/1‑150 (MQ=255)
                 gctgTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCtcacatc                                                                                                                                     <  2:7000527/150‑1 (MQ=255)
                 gctgTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCtcacatc                                                                                                                                     >  1:5098663/1‑150 (MQ=255)
                          aaCCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTc                                                                                                                            >  2:3866402/1‑150 (MQ=255)
                                   aGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTc                                                                                                                   <  1:6487606/150‑1 (MQ=255)
                                   aGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTc                                                                                                                   <  2:6936245/150‑1 (MQ=255)
                                   aGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTc                                                                                                                   <  1:369031/150‑1 (MQ=255)
                                          cGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATcc                                                                                                            <  2:8210648/150‑1 (MQ=255)
                                           gTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCt                                                                                                           >  1:3478001/1‑150 (MQ=255)
                                           gTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCt                                                                                                           >  2:3172342/1‑150 (MQ=255)
                                           gTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCt                                                                                                           >  2:5689348/1‑150 (MQ=255)
                                           gTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCt                                                                                                           <  2:1525091/150‑1 (MQ=255)
                                            ttCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTg                                                                                                          >  1:2066766/1‑150 (MQ=255)
                                            ttCAAGCAAAGCGTGGCAGACTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCAGCCTg                                                                                                          >  2:5282416/1‑150 (MQ=255)
                                             tctaGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGc                                                                                                         >  1:2564207/4‑150 (MQ=255)
                                             tCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGc                                                                                                         >  1:3594840/1‑150 (MQ=255)
                                                   aaaGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAAc                                                                                                   >  1:3139657/1‑150 (MQ=255)
                                                    aaGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAAcc                                                                                                  <  1:7962981/150‑1 (MQ=255)
                                                    aaGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAAcc                                                                                                  >  2:7976781/1‑150 (MQ=255)
                                                     aGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCGGTGCAaccat                                                                                                                                              <  1:78550/105‑2 (MQ=255)
                                                         tGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCa                                                                                             >  1:4892524/1‑150 (MQ=255)
                                                             aGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGcac                                                                                         <  2:1759496/150‑1 (MQ=255)
                                                                   tttCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGa                                                                                   >  1:813655/1‑150 (MQ=255)
                                                                         cAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTccc                                                                             >  2:2458537/1‑150 (MQ=255)
                                                                         cAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTccc                                                                             >  1:6599235/1‑150 (MQ=255)
                                                                         cAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTccc                                                                             >  2:6701676/1‑150 (MQ=255)
                                                                          aaaCGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTccc                                                                             <  2:2708543/149‑1 (MQ=255)
                                                                          aaaCGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTccc                                                                             >  1:2708543/1‑149 (MQ=255)
                                                                          aaaCGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCa                                                                            >  1:3948468/1‑150 (MQ=255)
                                                                          aaaCGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCa                                                                            <  2:4788895/150‑1 (MQ=255)
                                                                          aaaCGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCa                                                                            >  1:4061977/1‑150 (MQ=255)
                                                                          aaaCGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATACATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCa                                                                            >  1:5609936/1‑150 (MQ=255)
                                                                           aaCGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAg                                                                           <  1:7755148/150‑1 (MQ=255)
                                                                            aCGACCCAGCCTGAGGGACGTATTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAgg                                                                          <  1:173099/150‑1 (MQ=255)
                                                                                 ccAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCaga                                                                     >  1:6070473/1‑150 (MQ=255)
                                                                                 ccAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCaga                                                                     <  2:6490119/150‑1 (MQ=255)
                                                                                 ccAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCaga                                                                     <  2:2025368/150‑1 (MQ=255)
                                                                                 ccAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCaga                                                                     <  2:1076035/150‑1 (MQ=255)
                                                                                 ccAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAACACAGGTGATCTCCCAGGTCaga                                                                     <  1:1867558/150‑1 (MQ=255)
                                                                                    gCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCg                                                                  <  2:7714089/150‑1 (MQ=255)
                                                                                           ggACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGgctgct                                                           >  1:7144204/1‑150 (MQ=255)
                                                                                           ggACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGgctgct                                                           <  2:4387874/150‑1 (MQ=255)
                                                                                            gACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGctgctg                                                          >  1:7003830/1‑150 (MQ=255)
                                                                                               gTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGAc                                                       <  1:1608039/150‑1 (MQ=255)
                                                                                                tCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCAACAACCGGCCAGCACAGGTGAGCTCCCAGGGCAGGGCGGGCGGCGGGGCg                                                      >  2:6202884/1‑150 (MQ=255)
                                                                                                 cTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACCTCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGa                                                     <  2:448920/150‑1 (MQ=255)
                                                                                                 cTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGa                                                     >  1:4829220/1‑150 (MQ=255)
                                                                                                  ttATCAACAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAg                                                    <  2:8397827/150‑1 (MQ=255)
                                                                                                  ttATCAAAATAAGCATTTCAGGTTTTCAGCGACCTGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAg                                                    <  1:1908736/150‑1 (MQ=255)
                                                                                                  ttATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAg                                                    <  2:7214770/150‑1 (MQ=255)
                                                                                                  ttATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAg                                                    <  2:8662944/150‑1 (MQ=255)
                                                                                                     tCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCtt                                                 >  1:4230706/1‑150 (MQ=255)
                                                                                                      cAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCCCATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTtc                                                <  1:4309524/150‑1 (MQ=255)
                                                                                                      cAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTtc                                                >  2:7850030/1‑150 (MQ=255)
                                                                                                        aaaGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTtctc                                              <  1:4485696/150‑1 (MQ=255)
                                                                                                        aaaGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTtctc                                              <  2:3255091/150‑1 (MQ=255)
                                                                                                         aagaagCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCa                                             <  2:4239667/150‑1 (MQ=255)
                                                                                                         aagaagCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCa                                             <  1:1390780/150‑1 (MQ=255)
                                                                                                         aagaagCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCa                                             <  1:5814453/150‑1 (MQ=255)
                                                                                                         aagaagCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCa                                             <  2:2313229/150‑1 (MQ=255)
                                                                                                         aagaagCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCa                                             <  2:2066933/150‑1 (MQ=255)
                                                                                                         aagaagCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCa                                             <  1:8605306/150‑1 (MQ=255)
                                                                                                          agaagCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAg                                            <  1:8457300/150‑1 (MQ=255)
                                                                                                           gaagCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAgg                                           >  2:2832798/1‑150 (MQ=255)
                                                                                                               cATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCAtt                                       <  1:2421712/150‑1 (MQ=255)
                                                                                                                     aGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTgg                                 >  1:7694819/1‑150 (MQ=255)
                                                                                                                       gTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGcc                               >  2:2935542/1‑150 (MQ=255)
                                                                                                                         tttCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCaa                             >  2:7120301/1‑150 (MQ=255)
                                                                                                                         tttCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCaa                             <  1:3308016/150‑1 (MQ=255)
                                                                                                                          ttCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAg                            <  2:1467682/150‑1 (MQ=255)
                                                                                                                              gCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAAc                        >  2:2272673/1‑150 (MQ=255)
                                                                                                                                gACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACgt                      <  1:8077864/150‑1 (MQ=255)
                                                                                                                                gACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACgt                      >  1:2267185/1‑150 (MQ=255)
                                                                                                                                   cGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGtgtg                   >  2:6070788/1‑150 (MQ=255)
                                                                                                                                    ggTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGc                  >  2:6447272/1‑150 (MQ=255)
                                                                                                                                      tATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCaa                <  2:1547751/150‑1 (MQ=255)
                                                                                                                                      tATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCaa                >  2:7333742/1‑150 (MQ=255)
                                                                                                                                        tCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACt              >  2:3685914/1‑150 (MQ=255)
                                                                                                                                          ggtGGTTGCGGTGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTaa            >  1:5454904/3‑150 (MQ=255)
                                                                                                                                          cATGGTTGCACCGCAACCCCCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCCTGTTCTGGCCCAGGAACGGGGGCACCtcc            >  2:3726591/1‑148 (MQ=255)
                                                                                                                                          cATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTaa            <  1:6203441/150‑1 (MQ=255)
                                                                                                                                           gtGGTTGCGGTGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAg           >  1:2966570/2‑150 (MQ=255)
                                                                                                                                           gtGGTTGCGGTGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAg           >  1:293017/2‑150 (MQ=255)
                                                                                                                                           aTGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAg           >  1:4195711/1‑150 (MQ=255)
                                                                                                                                           aTGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAg           >  2:7443334/1‑150 (MQ=255)
                                                                                                                                           aTGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAg           >  2:6613565/1‑150 (MQ=255)
                                                                                                                                             ggTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAgcg         <  1:8776169/150‑1 (MQ=255)
                                                                                                                                                tGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGcgccg      <  2:8110981/150‑1 (MQ=255)
                                                                                                                                                  cACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGCGCCGCa    <  1:5381089/150‑1 (MQ=255)
                                                                                                                                                   aCCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTACATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGCGCCGCAg   >  2:8642437/1‑150 (MQ=255)
                                                                                                                                                    ccGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGAc                                                       <  1:1488226/97‑1 (MQ=255)
                                                                                                                                                    ccGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGCGCCGCAGa  >  2:464601/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACATCGACGCCATGACTGCTGTAGCAAACCCACCCAGGCTGACGTTCAAGCAAAGCGTGGCAGCCTGTTTCTCCAAACGACCCAGCCTGAGGGACGTCTTATCAAAAGAAGCATTTCAGGTTTTCAGCGACCGGTATCCATGGTTGCACCGCAACCACCCTCACATCACATCGCTCGATCCTTTCGTCATCCTGCATGAACCGGCCAGCACAGGTGATCTCCCAGGTCAGAGCGGGCTGCTGGACGAGCTTCTCAGGCATTTTCTGGCCAAGGAACGTGTGCAACTAAGCGCCGCAGA  >  NC_002947/5710977‑5711274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑