BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	450,923	C→A	6.6%	noncoding (117/127 nt)	PP_mr05 →	RsmY ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	450,923	0	C	A	6.6%	1336.8 / 53.5	399	noncoding (117/127 nt)	PP_mr05	RsmY ncRNA
Reads supporting (aligned to +/- strand): ref base C (184/188); new base A (9/18); total (193/206)
Fisher's exact test for biased strand distribution p-value = 1.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 415 total aligned reads displayed.

TTCTAAGATTGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATACCAGGCC  >  NC_002947/450774‑451071

   tAAGATTGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAGGCGCAAAGGACAGATCAAGGACGACGACGTAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTcggc                                                                                                                                                   >  2:1954082/1‑150 (MQ=255)

   tAAGATTGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTcggc                                                                                                                                                   >  1:5986524/1‑150 (MQ=255)

   tAAGATTGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTcggc                                                                                                                                                   >  2:2107711/1‑150 (MQ=255)

        ttGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGt                                                                                                                                              >  2:5301049/1‑150 (MQ=255)

          ggATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCCGAAGCGGGGttt                                                                                                                                            <  1:5137552/150‑1 (MQ=255)

           gATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGtttt                                                                                                                                           >  1:6380706/1‑150 (MQ=255)

           gATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGtttt                                                                                                                                           <  1:8361435/150‑1 (MQ=255)

            aTCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCCGAAGCGGGGTTTTc                                                                                                                                          <  2:4727255/150‑1 (MQ=255)

              ccACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCtt                                                                                                                                        <  1:2458119/150‑1 (MQ=255)

              ccACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCtt                                                                                                                                        <  1:8506260/150‑1 (MQ=255)

              ccACTGTTAATCCTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGAGCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCGCGCCGAAGCGGGGTTTTcca                                                                                                                                        <  2:4303515/150‑3 (MQ=255)

               cACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCttt                                                                                                                                       <  1:7986259/150‑1 (MQ=255)

               cACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCttt                                                                                                                                       <  1:3714380/150‑1 (MQ=255)

                aCTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTtg                                                                                                                                      >  1:671674/1‑150 (MQ=255)

                 cTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTtgc                                                                                                                                     <  2:8672986/150‑1 (MQ=255)

                  tGTTAATCTTTACCACATGGACGTTGCGCAGGACGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTtgcg                                                                                                                                    >  1:1590606/1‑150 (MQ=255)

                  tGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACATGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTtgcg                                                                                                                                    >  2:482097/1‑150 (MQ=255)

                   gTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTtgcgt                                                                                                                                   <  1:6263202/150‑1 (MQ=255)

                    ttAATCTTTACCACATGGACGTCGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGACGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTtgcgtg                                                                                                                                  <  1:73061/150‑1 (MQ=255)

                       aTCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCATCGGCGGGGTTTTCTTTgcgtgcgt                                                                                                                               >  1:1431898/1‑150 (MQ=255)

                        tCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGcgtgcgtg                                                                                                                              >  2:5257538/1‑150 (MQ=255)

                        tCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGcgtgcgtg                                                                                                                              >  1:2305718/1‑150 (MQ=255)

                          tttACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGtt                                                                                                                            >  1:7340683/1‑150 (MQ=255)

                           ttACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGttt                                                                                                                           <  1:2801009/150‑1 (MQ=255)

                             aCCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGttttt                                                                                                                         <  2:5125554/150‑1 (MQ=255)

                              ccACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTc                                                                                                                        >  1:4098750/1‑150 (MQ=255)

                               cacaTGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCa                                                                                                                       >  1:1836136/1‑150 (MQ=255)

                                 caTGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAgg                                                                                                                     >  2:2679973/1‑150 (MQ=255)

                                     gACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAg                                                                                                                 >  1:4864439/1‑150 (MQ=255)

                                        gTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGcac                                                                                                              >  2:3610010/1‑150 (MQ=255)

                                        gTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGcac                                                                                                              >  1:6159025/1‑150 (MQ=255)

                                          tGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACAt                                                                                                            >  1:3548624/1‑150 (MQ=255)

                                           gcgcAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATc                                                                                                           >  2:3724382/1‑150 (MQ=255)

                                           gcgcAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATc                                                                                                           <  2:3166485/150‑1 (MQ=255)

                                           gcgcAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATc                                                                                                           >  2:6772252/1‑150 (MQ=255)

                                               aGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGc                                                                                                       <  2:8184263/150‑1 (MQ=255)

                                                ggAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGc                                                                                                       >  2:3477064/1‑150 (MQ=255)

                                                ggAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCg                                                                                                      <  1:3955554/150‑1 (MQ=255)

                                                 gAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCgg                                                                                                     >  1:5903689/1‑150 (MQ=255)

                                                 gAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCgg                                                                                                     <  1:811507/150‑1 (MQ=255)

                                                 gAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCgg                                                                                                     <  2:2324964/150‑1 (MQ=255)

                                                  aaGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGt                                                                                                    <  2:1737092/150‑1 (MQ=255)

                                                   aGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTg                                                                                                   <  1:6994572/150‑1 (MQ=255)

                                                    gcgcAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGa                                                                                                  <  1:8284420/150‑1 (MQ=255)

                                                     cgcAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGAt                                                                                                 <  1:3273305/150‑1 (MQ=255)

                                                        aaaGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCt                                                                                              >  2:8090130/1‑150 (MQ=255)

                                                            gACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCa                                                                                          >  2:6952784/1‑150 (MQ=255)

                                                              cAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATc                                                                                        <  1:5575514/150‑1 (MQ=255)

                                                               aGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCa                                                                                       <  1:5840119/150‑1 (MQ=255)

                                                                 aTCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAgg                                                                                     <  2:5405170/150‑1 (MQ=255)

                                                                    aaGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGa                                                                                  <  1:5306343/150‑1 (MQ=255)

                                                                     aGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAg                                                                                 >  2:7348279/1‑150 (MQ=255)

                                                                        acgacgacgAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGcgt                                                                              <  1:496544/150‑1 (MQ=255)

                                                                          gacgacgaAGTACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGcgtcc                                                                            <  2:2441326/150‑1 (MQ=255)

                                                                          gacgacgaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGcgtcc                                                                            <  2:1427550/150‑1 (MQ=255)

                                                                            cgacgaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGcgtccgt                                                                          <  2:6510635/150‑1 (MQ=255)

                                                                             gacgaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGcgtccgtc                                                                         >  2:7629973/1‑150 (MQ=255)

                                                                             gacgaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGcgtccgtc                                                                         <  2:3298595/150‑1 (MQ=255)

                                                                              acgaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGtct                                                                        <  1:1369003/150‑1 (MQ=255)

                                                                                gaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTctct                                                                      >  1:995770/1‑150 (MQ=255)

                                                                                gaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTctct                                                                      >  1:7718358/1‑150 (MQ=255)

                                                                                gaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTctct                                                                      >  1:7050280/1‑150 (MQ=255)

                                                                                 aaGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCtt                                                                     >  2:5759368/1‑150 (MQ=255)

                                                                                  aGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTtc                                                                    >  2:4444033/1‑150 (MQ=255)

                                                                                     accaccTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTtcctc                                                                 <  1:3096505/150‑1 (MQ=255)

                                                                                       caccTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCa                                                               <  2:6314468/150‑1 (MQ=255)

                                                                                       caccTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCa                                                               >  2:6120801/1‑150 (MQ=255)

                                                                                        accTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAg                                                              <  2:5232967/150‑1 (MQ=255)

                                                                                          cTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCa                                                            >  1:102321/1‑150 (MQ=255)

                                                                                               aGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTg                                                       >  2:7937816/1‑150 (MQ=255)

                                                                                                     gCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCgcagc                                                 <  1:1779767/150‑1 (MQ=255)

                                                                                                          gCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTc                                            >  1:3464087/1‑150 (MQ=255)

                                                                                                             aTACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCcac                                         >  2:8615449/1‑150 (MQ=255)

                                                                                                              tACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCcaca                                        >  2:3957636/1‑150 (MQ=255)

                                                                                                                 ggATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACagag                                     <  1:8792778/150‑1 (MQ=255)

                                                                                                                  gATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAgg                                    >  2:720395/1‑150 (MQ=255)

                                                                                                                   aTGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGc                                   <  2:423114/150‑1 (MQ=255)

                                                                                                                     gTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCct                                 <  1:8615449/150‑1 (MQ=255)

                                                                                                                      tCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTAGGCGGGCTTTTCTTTGCGAGAGTGTTTTTCAGGCCAGCACATAAAGCTCTGAAGCTCACATCAGGCGAGCGTCCTTCTATTACACCACCAACTTGCGCAGCAGCTACAAAGAGGcatc                                >  2:4950517/1‑147 (MQ=255)

                                                                                                                          aGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTgg                            >  2:8767770/1‑150 (MQ=255)

                                                                                                                           gggAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGgc                           >  2:5324488/1‑150 (MQ=255)

                                                                                                                             gAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGgcgc                         >  2:5490783/1‑150 (MQ=255)

                                                                                                                               aaCAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTg                       <  1:6601944/150‑1 (MQ=255)

                                                                                                                                aCAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTgg                      <  1:713555/150‑1 (MQ=255)

                                                                                                                                aCAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTgg                      <  1:5751268/150‑1 (MQ=255)

                                                                                                                                aCAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTgg                      <  2:1605011/150‑1 (MQ=255)

                                                                                                                                aCAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTgg                      <  1:420291/150‑1 (MQ=255)

                                                                                                                                aCAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTgg                      <  1:4733206/150‑1 (MQ=255)

                                                                                                                                aCAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTgg                      <  1:4428161/150‑1 (MQ=255)

                                                                                                                                    tCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCAt                  >  1:4528336/1‑150 (MQ=255)

                                                                                                                                     aaagAAAA‑CCCCGCCGAAGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATc                 >  2:1221322/4‑150 (MQ=255)

                                                                                                                                      tGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGa                                                                                  <  2:5867993/84‑1 (MQ=255)

                                                                                                                                        gAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATcgcg              <  1:2023710/150‑1 (MQ=255)

                                                                                                                                         aaaa‑CCCCGCCGAAGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGa             >  2:2935787/1‑150 (MQ=255)

                                                                                                                                               cccGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATAc        >  2:2780887/1‑150 (MQ=255)

                                                                                                                                               cccGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATAc        >  2:7095797/1‑150 (MQ=255)

                                                                                                                                                 cGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATACCa      >  1:5476167/1‑150 (MQ=255)

                                                                                                                                                    ttCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCCTCGCGAAATACCAGGc   >  1:4675145/1‑150 (MQ=255)

                                                                                                                                                    ttCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATACCAGGc   <  1:2679973/150‑1 (MQ=255)

                                                                                                                                                     tCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATACCAGGcc  <  2:7691806/150‑1 (MQ=255)

TTCTAAGATTGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATACCAGGCC  >  NC_002947/450774‑451071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`