BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	450,924	G→A	6.5%	noncoding (118/127 nt)	PP_mr05 →	RsmY ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	450,924	0	G	A	6.5%	1249.0 / 46.6	399	noncoding (118/127 nt)	PP_mr05	RsmY ncRNA
Reads supporting (aligned to +/- strand): ref base G (184/188); new base A (8/18); total (193/206)
Fisher's exact test for biased strand distribution p-value = 7.07e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 415 total aligned reads displayed.

TCTAAGATTGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATACCAGGCCGA  >  NC_002947/450775‑451073

 cTAAGATTGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTcgg                                                                                                                                                      >  2:1943717/1‑150 (MQ=255)

     gATTGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCggg                                                                                                                                                  <  1:6471991/150‑1 (MQ=255)

       ttGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGt                                                                                                                                                >  2:5301049/1‑150 (MQ=255)

         ggATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCCGAAGCGGGGttt                                                                                                                                              <  1:5137552/150‑1 (MQ=255)

          gATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGtttt                                                                                                                                             >  2:3166414/1‑150 (MQ=255)

           aTCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTc                                                                                                                                            <  1:731784/150‑1 (MQ=255)

           aTCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCCGAAGCGGGGTTTTc                                                                                                                                            <  2:4727255/150‑1 (MQ=255)

             ccACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCtt                                                                                                                                          <  1:2458119/150‑1 (MQ=255)

             ccACTGTTAATCCTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGAGCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCGCGCCGAAGCGGGGTTTTcca                                                                                                                                          <  2:4303515/150‑3 (MQ=255)

              cACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCttt                                                                                                                                         <  1:3714380/150‑1 (MQ=255)

               aCTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTAGAAAA‑CCCCGCCGAAGCGGGGTTTTccaga                                                                                                                                        <  1:8293854/150‑5 (MQ=255)

                cTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTtgc                                                                                                                                       <  2:8672986/150‑1 (MQ=255)

                 tGTTAATCTTTACCACATGGACGTTGCGCAGGACGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTtgcg                                                                                                                                      >  1:1590606/1‑150 (MQ=255)

                 tGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACATGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTtgcg                                                                                                                                      >  2:482097/1‑150 (MQ=255)

                      aTCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCATCGGCGGGGTTTTCTTTgcgtgcgt                                                                                                                                 >  1:1431898/1‑150 (MQ=255)

                       tCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGcgtgcgtg                                                                                                                                >  1:2305718/1‑150 (MQ=255)

                       tCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGcgtgcgtg                                                                                                                                >  1:5819272/1‑150 (MQ=255)

                       tCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGcgtgcgtg                                                                                                                                >  2:5257538/1‑150 (MQ=255)

                          ttACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACCGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGttt                                                                                                                             <  2:3193604/150‑1 (MQ=255)

                          ttACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGttt                                                                                                                             <  1:2801009/150‑1 (MQ=255)

                            aCCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGttttt                                                                                                                           <  2:5125554/150‑1 (MQ=255)

                             ccACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTc                                                                                                                          <  2:2070683/150‑1 (MQ=255)

                             ccACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTc                                                                                                                          >  1:4098750/1‑150 (MQ=255)

                              cacaTGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCa                                                                                                                         >  1:7440617/1‑150 (MQ=255)

                              cacaTGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCa                                                                                                                         >  1:1836136/1‑150 (MQ=255)

                                    gACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAg                                                                                                                   >  1:4864439/1‑150 (MQ=255)

                                     aCGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGc                                                                                                                  <  1:8456799/150‑1 (MQ=255)

                                       gTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGcac                                                                                                                >  2:3610010/1‑150 (MQ=255)

                                         tGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACAt                                                                                                              >  1:3548624/1‑150 (MQ=255)

                                          gcgcAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATc                                                                                                             >  2:6772252/1‑150 (MQ=255)

                                          gcgcAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATc                                                                                                             >  2:3724382/1‑150 (MQ=255)

                                              aGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGc                                                                                                         <  2:8184263/150‑1 (MQ=255)

                                              aGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGc                                                                                                         >  1:862819/1‑150 (MQ=255)

                                               ggAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGc                                                                                                         >  2:3477064/1‑150 (MQ=255)

                                               ggAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCg                                                                                                        <  1:3955554/150‑1 (MQ=255)

                                                gAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCATGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCgg                                                                                                       <  1:811701/150‑1 (MQ=255)

                                                    cgcAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGAt                                                                                                   <  1:3273305/150‑1 (MQ=255)

                                                      cAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGc                                                                                                 <  1:7841760/150‑1 (MQ=255)

                                                       aaaGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCt                                                                                                >  2:8090130/1‑150 (MQ=255)

                                                           gACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCa                                                                                            >  2:6952784/1‑150 (MQ=255)

                                                           gACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTATTCAGGCCAGCACATCCAGCGGTGATGCTCCCa                                                                                            >  2:1157442/1‑150 (MQ=255)

                                                             cAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATc                                                                                          <  1:5575514/150‑1 (MQ=255)

                                                                aTCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAgg                                                                                       <  2:5405170/150‑1 (MQ=255)

                                                                   aaGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGa                                                                                    <  1:5306343/150‑1 (MQ=255)

                                                                    aGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAg                                                                                   >  2:7348279/1‑150 (MQ=255)

                                                                     ggACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGc                                                                                  >  1:7127881/1‑150 (MQ=255)

                                                                     ggACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGc                                                                                  >  1:6108102/1‑150 (MQ=255)

                                                                       acgacgacgAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGcgt                                                                                <  1:496544/150‑1 (MQ=255)

                                                                         gacgacgaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGcgtcc                                                                              <  2:1414995/150‑1 (MQ=255)

                                                                           cgacgaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGcgtccgt                                                                            <  2:6510635/150‑1 (MQ=255)

                                                                            gacgaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGcgtccgtc                                                                           >  2:7629973/1‑150 (MQ=255)

                                                                             acgaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGtct                                                                          <  1:1369003/150‑1 (MQ=255)

                                                                               gaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTctct                                                                        >  1:6250267/1‑150 (MQ=255)

                                                                                aaGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCtt                                                                       >  1:5949664/1‑150 (MQ=255)

                                                                                aaGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCtt                                                                       >  2:5759368/1‑150 (MQ=255)

                                                                                 aGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTtc                                                                      >  2:4444033/1‑150 (MQ=255)

                                                                                    accaccTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTtcctc                                                                   <  1:3096505/150‑1 (MQ=255)

                                                                                      caccTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCa                                                                 <  2:6314468/150‑1 (MQ=255)

                                                                                      caccTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCa                                                                 >  2:6120801/1‑150 (MQ=255)

                                                                                       accTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAg                                                                <  2:5232967/150‑1 (MQ=255)

                                                                                         cTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCa                                                              >  1:102321/1‑150 (MQ=255)

                                                                                             cAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGt                                                          <  1:6882327/150‑1 (MQ=255)

                                                                                              aGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTg                                                         >  2:7937816/1‑150 (MQ=255)

                                                                                                gATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTgcg                                                       <  1:7044869/150‑1 (MQ=255)

                                                                                                  tGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCgca                                                     >  2:2275756/1‑150 (MQ=255)

                                                                                                    gCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCgcagc                                                   <  1:1779767/150‑1 (MQ=255)

                                                                                                       gggCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCagcagc                                                <  1:958223/150‑1 (MQ=255)

                                                                                                         gCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTc                                              >  1:3464087/1‑150 (MQ=255)

                                                                                                            aTACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCcac                                           >  2:8615449/1‑150 (MQ=255)

                                                                                                             tACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCcaca                                          >  2:3957636/1‑150 (MQ=255)

                                                                                                                ggATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACagag                                       >  1:8612015/1‑150 (MQ=255)

                                                                                                                 gATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAgg                                      >  1:8057513/1‑150 (MQ=255)

                                                                                                                 gATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAgg                                      >  2:720395/1‑150 (MQ=255)

                                                                                                                  aTGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGc                                     <  2:423114/150‑1 (MQ=255)

                                                                                                                     tCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTAGGCGGGCTTTTCTTTGCGAGAGTGTTTTTCAGGCCAGCACATAAAGCTCTGAAGCTCACATCAGGCGAGCGTCCTTCTATTACACCACCAACTTGCGCAGCAGCTACAAAGAGGcatc                                  >  2:4950517/1‑147 (MQ=255)

                                                                                                                      cacaGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCctgc                                 <  2:2851456/150‑1 (MQ=255)

                                                                                                                       acaGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCctgct                                >  2:2427092/1‑150 (MQ=255)

                                                                                                                       acaGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCctgct                                >  2:2767707/1‑150 (MQ=255)

                                                                                                                         aGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTgg                              >  2:8767770/1‑150 (MQ=255)

                                                                                                                          gggAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGgc                             >  2:5324488/1‑150 (MQ=255)

                                                                                                                           ggAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGgcg                            >  2:260476/1‑150 (MQ=255)

                                                                                                                            gAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGgcgc                           >  2:5490783/1‑150 (MQ=255)

                                                                                                                              aaCAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTg                         <  1:6345460/150‑1 (MQ=255)

                                                                                                                               aCAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTgg                        <  1:5751268/150‑1 (MQ=255)

                                                                                                                               aCAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTgg                        <  1:7228605/150‑1 (MQ=255)

                                                                                                                               aCAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTgg                        <  1:4733206/150‑1 (MQ=255)

                                                                                                                               aCAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTgg                        <  1:4428161/150‑1 (MQ=255)

                                                                                                                               aCAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTgg                        <  1:420291/150‑1 (MQ=255)

                                                                                                                                   tCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCAt                    >  1:4528336/1‑150 (MQ=255)

                                                                                                                                     tGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGa                                                                                    >  1:5867993/1‑84 (MQ=255)

                                                                                                                                     tGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGa                                                                                    <  2:5867993/84‑1 (MQ=255)

                                                                                                                                       gAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATcgcg                <  1:2023710/150‑1 (MQ=255)

                                                                                                                                              cccGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATAc          >  2:7095797/1‑150 (MQ=255)

                                                                                                                                              cccGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATAc          <  2:1646899/150‑1 (MQ=255)

                                                                                                                                              cccGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATAc          >  2:1556702/1‑150 (MQ=255)

                                                                                                                                               ccGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATAcc         >  1:661758/1‑150 (MQ=255)

                                                                                                                                                cGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATACCa        >  1:5476167/1‑150 (MQ=255)

                                                                                                                                                   ttCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCCTCGCGAAATACCAGGc     >  1:4675145/1‑150 (MQ=255)

                                                                                                                                                   ttCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATACCAGGc     <  1:2679973/150‑1 (MQ=255)

                                                                                                                                                    tCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATACCAGGcc    <  2:7691806/150‑1 (MQ=255)

TCTAAGATTGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATACCAGGCCGA  >  NC_002947/450775‑451073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`