BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,891,774	C→G	5.5%	A83P (GCA→CCA)	PP_5161 ←	lysophospholipase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,891,774	0	C	G	5.5%	1021.7 / 33.6	301	A83P (GCA→CCA)	PP_5161	lysophospholipase
Reads supporting (aligned to +/- strand): ref base C (133/151); new base G (10/7); total (143/158)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 319 total aligned reads displayed.

GCTGGACAGGCCGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGTGCGCAGGCGGT  >  NC_002947/5891625‑5891923

gcTGGACAGGCCGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCAtgc                                                                                                                                                       <  1:8253136/150‑1 (MQ=255)

  tGGACAGGCCGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCAtgcct                                                                                                                                                     <  2:6198738/150‑1 (MQ=255)

   ggACAGGCCGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCAtgcctg                                                                                                                                                    >  1:1250543/1‑150 (MQ=255)

   ggACAGGCCGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCAtgcctg                                                                                                                                                    >  1:3844820/1‑150 (MQ=255)

      cAGGCCGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGccc                                                                                                                                                 <  2:2917785/150‑1 (MQ=255)

        ggCCGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGGCATGGCTGCCCGa                                                                                                                                               <  1:4313398/150‑1 (MQ=255)

        ggCCGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGa                                                                                                                                               <  2:6821607/150‑1 (MQ=255)

         gCCGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGGCATGGCTGCCCGag                                                                                                                                              <  2:5727006/150‑2 (MQ=255)

         gCCGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGAc                                                                                                                                              <  2:6490832/150‑1 (MQ=255)

         gCCGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGAc                                                                                                                                              >  2:2377443/1‑150 (MQ=255)

          ccGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGAcc                                                                                                                                             <  2:427381/150‑1 (MQ=255)

          ccGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGAcc                                                                                                                                             <  1:6451574/150‑1 (MQ=255)

              ggCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGTTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGt                                                                                                                                         <  2:5178551/150‑1 (MQ=255)

              ggCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGt                                                                                                                                         <  2:3370718/150‑1 (MQ=255)

               gCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTc                                                                                                                                        <  1:8578520/150‑1 (MQ=255)

                ccGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCg                                                                                                                                       >  1:1049501/1‑150 (MQ=255)

                  gggCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGaa                                                                                                                                     <  2:5884741/150‑1 (MQ=255)

                   ggCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCAtgcctgc                                                                                                                                                   >  2:194450/1‑135 (MQ=255)

                     cAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAAccc                                                                                                                                  <  2:55884/150‑1 (MQ=255)

                      aGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAAcccc                                                                                                                                 <  2:510993/150‑1 (MQ=255)

                      aGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAAcccc                                                                                                                                 <  1:1447977/150‑1 (MQ=255)

                      aGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAAcccc                                                                                                                                 <  2:8131319/150‑1 (MQ=255)

                          cacaGCTGATCACGGCGAAACCCTGCGGGAGTGCCCACTCGATCACAGGGCGGGAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCCGCCCTGCCGGCCCGACCACGTCGAACCCCGcgg                                                                                                                             >  1:2042139/1‑148 (MQ=255)

                           acaGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGc                                                                                                                            >  2:5504944/1‑150 (MQ=255)

                                 gATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGaaa                                                                                                                      <  2:1104878/150‑1 (MQ=255)

                                 gATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGaaa                                                                                                                      <  1:5916659/150‑1 (MQ=255)

                                     aCGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTa                                                                                                                  <  1:978047/150‑1 (MQ=255)

                                     aCGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTa                                                                                                                  >  2:6669247/1‑150 (MQ=255)

                                       ggCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGGTCGGGCAGGCATGGCTGCCCGAGCAgc                                                                                                                                          >  1:5050728/1‑123 (MQ=39)

                                       ggCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGGTCGGGCAGGCATGGCTGCCCGAGCAgc                                                                                                                                          <  2:5050728/124‑2 (MQ=39)

                                               ccTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCcagccagc                                                                                                        <  2:3659188/150‑1 (MQ=255)

                                                cTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCt                                                                                                       <  1:5578857/150‑1 (MQ=255)

                                                  gCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCtgt                                                                                                     <  2:3992256/150‑1 (MQ=255)

                                                  gCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCtgt                                                                                                     <  1:3959591/150‑1 (MQ=255)

                                                  gCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCtgt                                                                                                     <  1:4153720/150‑1 (MQ=255)

                                                  gCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCtgt                                                                                                     <  1:693063/150‑1 (MQ=255)

                                                  gCTTGAGCGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCtgt                                                                                                     <  1:4592982/150‑1 (MQ=255)

                                                      gAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGc                                                                                                 <  2:470121/150‑1 (MQ=255)

                                                        gTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCctgctggcggtaa                                                                                               <  2:7730551/150‑8 (MQ=255)

                                                        gTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCg                                                                                               <  2:1185765/150‑1 (MQ=255)

                                                         tGCCCACTCTATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCgg                                                                                              <  1:289958/150‑1 (MQ=255)

                                                           cccACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCa                                                                                            >  2:2272257/1‑150 (MQ=255)

                                                            ccACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAg                                                                                           >  1:899284/1‑150 (MQ=255)

                                                             cACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAgg                                                                                          >  2:6409527/1‑150 (MQ=255)

                                                             cACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAgg                                                                                          >  1:7354194/1‑150 (MQ=255)

                                                                   aTCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAgg                                                                                    <  1:2964093/150‑1 (MQ=255)

                                                                     cacaTGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGcc                                                                                  >  1:8160804/1‑150 (MQ=255)

                                                                        aTGTCGGTAGAGGCCCATGTGTTCGTTATAGCCGTGCAGCATGATCAGCGTCGCGGTTGGCTGCTCGGGGAGCCTTGCCTGCCCGAGCAGTTAGAAACCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCGGGCAGGTTCAGGCCTTa                                                                               <  2:2561282/150‑1 (MQ=255)

                                                                        aTGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATa                                                                               >  1:8342666/1‑150 (MQ=255)

                                                                          gTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGa                                                                             >  1:3736642/1‑150 (MQ=255)

                                                                             ggTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAgc                                                                          >  1:7730563/1‑150 (MQ=255)

                                                                             ggTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAgc                                                                          >  1:6374383/1‑150 (MQ=255)

                                                                                  agGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGGCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTgg                                                                     <  2:2645830/150‑1 (MQ=255)

                                                                                    gCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTa                                                                   >  2:276727/1‑150 (MQ=255)

                                                                                    gCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTa                                                                   >  2:5205821/1‑150 (MQ=255)

                                                                                    gCCCATGTGGCCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTa                                                                   <  1:3068579/150‑1 (MQ=255)

                                                                                      ccATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGt                                                                 >  2:7285994/1‑150 (MQ=255)

                                                                                      ccATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGGGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGt                                                                 >  2:3905059/1‑150 (MQ=255)

                                                                                              tCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCt                                                         <  2:8230957/150‑1 (MQ=255)

                                                                                              tCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCt                                                         >  2:8484058/1‑150 (MQ=255)

                                                                                              tCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCt                                                         <  2:2946372/150‑1 (MQ=255)

                                                                                              tCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCt                                                         >  2:5367236/1‑150 (MQ=255)

                                                                                                gTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTCGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTtg                                                       >  1:4031122/1‑150 (MQ=255)

                                                                                                  aaTAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTtgtg                                                     >  1:3570198/1‑150 (MQ=255)

                                                                                                   aTAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGc                                                    <  2:3088868/150‑1 (MQ=255)

                                                                                                   aTAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGc                                                    <  1:2707406/150‑1 (MQ=255)

                                                                                                      gCCGTGCAGCAGAAACCGCGTCGCGCCTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGa                                                 <  1:5189863/150‑1 (MQ=255)

                                                                                                        cGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACa                                               <  2:3708252/150‑1 (MQ=255)

                                                                                                              gcagGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGtggct                                         <  1:7070011/150‑1 (MQ=255)

                                                                                                               cagGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGtggctg                                        >  1:1521267/1‑150 (MQ=255)

                                                                                                                    aCAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAg                                   <  1:331790/150‑1 (MQ=255)

                                                                                                                     cAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAgt                                  <  2:8771468/150‑1 (MQ=255)

                                                                                                                     cAGCGTCGCGCCTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGGTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAgt                                  <  2:3844820/150‑1 (MQ=255)

                                                                                                                      aGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAgtg                                 <  1:6859785/150‑1 (MQ=255)

                                                                                                                      aGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAgtg                                 >  1:5079717/1‑150 (MQ=255)

                                                                                                                      aGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAgtg                                 <  1:6044706/150‑1 (MQ=255)

                                                                                                                        cGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAgtggt                               <  1:2342248/150‑1 (MQ=255)

                                                                                                                         gTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAgtggtg                              >  1:8694597/1‑150 (MQ=255)

                                                                                                                           cgcgCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGaa                            <  2:7339146/150‑1 (MQ=255)

                                                                                                                              ttcgacctgctCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACgg                         >  2:3501635/7‑150 (MQ=255)

                                                                                                                               cTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGgc                        >  1:1676599/1‑150 (MQ=255)

                                                                                                                                ttGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTa                                                                                                                  >  2:3636069/1‑59 (MQ=255)

                                                                                                                                 tGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGgcgc                      <  2:7195299/150‑1 (MQ=255)

                                                                                                                                    ctggtCGGGCAGGCATGGCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAg                   >  1:6143190/1‑150 (MQ=255)

                                                                                                                                     tggtCGGGCAGGCATGGCTGCCCGAGCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAgg                  >  2:4496171/4‑150 (MQ=255)

                                                                                                                                      gctCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGc                 <  2:6995850/150‑1 (MQ=255)

                                                                                                                                      gctCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGc                 <  1:4401062/150‑1 (MQ=255)

                                                                                                                                      gctCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGc                 >  1:4941653/1‑150 (MQ=255)

                                                                                                                                       ctCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCg                <  1:1317220/150‑1 (MQ=255)

                                                                                                                                        tCGGGCAGCCATGCCTGCCTGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCgg               >  1:7842164/1‑150 (MQ=255)

                                                                                                                                        tCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCgg               >  1:726059/1‑150 (MQ=255)

                                                                                                                                        tCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCgg               >  1:257670/1‑150 (MQ=255)

                                                                                                                                        tCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCgg               >  2:922052/1‑150 (MQ=255)

                                                                                                                                        tCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGATTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCgg               <  1:2213072/150‑1 (MQ=255)

                                                                                                                                          gggCAGGCATGGCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTCGTCCTGCGCTTGTACCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGt             >  1:1885293/1‑150 (MQ=255)

                                                                                                                                          gggCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGt             <  2:2888486/150‑1 (MQ=255)

                                                                                                                                           ggCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCGACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATCGACGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGTg            <  2:4956575/150‑1 (MQ=255)

                                                                                                                                            gCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGTgc           >  2:7700152/1‑150 (MQ=255)

                                                                                                                                                ccATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCTGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGTGCGCAg       <  2:1331635/150‑1 (MQ=255)

                                                                                                                                                   tgcctgccCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGATTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGTGCGCAGGCg    <  1:4861104/150‑1 (MQ=255)

GCTGGACAGGCCGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGGCAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGTGCGCAGGCGGT  >  NC_002947/5891625‑5891923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`