BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,974,735	G→A	7.4%	L522L (CTG→TTG)	PP_5236 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,974,735	0	G	A	7.4%	944.7 / 43.0	309	L522L (CTG→TTG)	PP_5236	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (137/149); new base A (5/18); total (142/167)
Fisher's exact test for biased strand distribution p-value = 1.68e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 315 total aligned reads displayed.

ACCAAACCGGCGTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTCAG  >  NC_002947/5974586‑5974882

 acAAACCGGCGTAACAAAATCCCCAGGTGCAACAGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCAAGCGCAGGCAGGCCAgg                                                                                                                                                    >  1:3880140/2‑150 (MQ=255)

   aaaCCGGCGTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGtt                                                                                                                                                  <  1:5682649/150‑1 (MQ=255)

   aaaCCGGCGTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGtt                                                                                                                                                  <  2:759699/150‑1 (MQ=255)

   aaaCCGGCGTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGtt                                                                                                                                                  <  1:4777434/150‑1 (MQ=255)

   aaaCCGGCGTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGtt                                                                                                                                                  >  2:3446646/1‑150 (MQ=255)

     aCCGGCGTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGt                                                                                                                                                <  1:5567779/150‑1 (MQ=255)

        ggCGTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACt                                                                                                                                             >  1:8323531/1‑150 (MQ=255)

         gCGTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACtt                                                                                                                                            <  2:7382312/150‑1 (MQ=255)

              aCAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcct                                                                                                                                       >  2:8310913/1‑150 (MQ=255)

              aCAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcct                                                                                                                                       >  2:2880872/1‑150 (MQ=255)

               cAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctg                                                                                                                                      >  1:1250702/1‑150 (MQ=255)

                aaaaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctgc                                                                                                                                     <  2:4470722/150‑1 (MQ=255)

                aaaaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctgc                                                                                                                                     <  2:7945605/150‑1 (MQ=255)

                aaaaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGAGCAGGCAGGCCAAGTACAACCTGgcctgc                                                                                                                                     <  1:4045826/150‑1 (MQ=255)

                 tgcaccccAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAAGTACAACCTGgcctgcc                                                                                                                                    <  2:5630685/146‑1 (MQ=255)

                 aaaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctgcc                                                                                                                                    <  1:236512/150‑1 (MQ=255)

                  aaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAAGTACAACCTGgcctgcct                                                                                                                                   <  2:502306/150‑1 (MQ=255)

                    tCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCctgcctgc                                                                                                                                 <  2:5490535/150‑1 (MQ=255)

                    tCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCctgcctgc                                                                                                                                 >  1:2438982/1‑150 (MQ=255)

                       ccAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCtgctgc                                                                                                                              >  2:473385/1‑150 (MQ=255)

                       ccAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCtgctgc                                                                                                                              >  1:3770660/1‑150 (MQ=255)

                       ccAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCtgctgc                                                                                                                              >  1:8665530/1‑150 (MQ=255)

                         aGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGccc                                                                                                                            <  2:6232150/150‑1 (MQ=255)

                             gCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCt                                                                                                                        <  2:4440872/150‑1 (MQ=255)

                                  cgcgCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAgg                                                                                                                                                    >  1:1744500/1‑117 (MQ=255)

                                   gcgcACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCg                                                                                                                  <  2:1323681/150‑1 (MQ=255)

                                         acGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCt                                                                                                            >  2:2821730/1‑150 (MQ=255)

                                          cGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTg                                                                                                           <  2:5663558/150‑1 (MQ=255)

                                           gCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGc                                                                                                          >  1:7924148/1‑150 (MQ=255)

                                           gCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGc                                                                                                          >  2:1728767/1‑150 (MQ=255)

                                           gCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCCCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGc                                                                                                          >  2:5592001/1‑150 (MQ=255)

                                            cATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCa                                                                                                         <  2:3823462/150‑1 (MQ=255)

                                             aTTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAg                                                                                                        >  1:6577818/1‑150 (MQ=255)

                                                ggTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCTGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCa                                                                                                     <  1:4895757/150‑1 (MQ=255)

                                                 gTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAg                                                                                                    >  2:6901971/1‑150 (MQ=255)

                                                    tGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTAc                                                                                                 >  1:3275851/1‑150 (MQ=255)

                                                    tGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTAc                                                                                                 >  2:3284242/1‑150 (MQ=255)

                                                       ccGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCg                                                                                              >  2:1469852/1‑150 (MQ=255)

                                                       ccGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCg                                                                                              >  2:3666902/1‑150 (MQ=255)

                                                         gTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGc                                                                                            >  2:2284615/1‑150 (MQ=255)

                                                              ccAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCt                                                                                       >  1:7220262/1‑150 (MQ=255)

                                                               cAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTc                                                                                      <  2:2633716/150‑1 (MQ=255)

                                                               cAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTc                                                                                      <  1:5247932/150‑1 (MQ=255)

                                                                 gcgcGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGt                                                                                    <  1:1304626/150‑1 (MQ=255)

                                                                         aCAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAgg                                                                            >  2:3721191/1‑150 (MQ=255)

                                                                         aCAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAgg                                                                            >  2:2465598/1‑150 (MQ=255)

                                                                           aaGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTa                                                                          <  1:4603171/150‑1 (MQ=255)

                                                                            aGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTaa                                                                         <  1:4363452/150‑1 (MQ=255)

                                                                             gCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAAt                                                                        >  2:1825477/1‑150 (MQ=255)

                                                                               aGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATgc                                                                      <  2:3984475/150‑1 (MQ=255)

                                                                               aGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATgc                                                                      >  1:2000524/1‑150 (MQ=255)

                                                                               aGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATgc                                                                      >  1:6466019/1‑150 (MQ=255)

                                                                                    ccTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTTTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCgg                                                                 <  1:3152883/150‑1 (MQ=255)

                                                                                     cTGACCGCCAGCTTGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCTGGCAGGCCAGTTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCggg                                                                <  1:7375215/150‑1 (MQ=255)

                                                                                     cTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCggg                                                                >  1:1376636/1‑150 (MQ=255)

                                                                                     cTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCggg                                                                <  2:5515645/150‑1 (MQ=255)

                                                                                     cTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGCTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCggg                                                                <  2:3789718/150‑1 (MQ=255)

                                                                                      tGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCgggg                                                               >  2:851162/1‑150 (MQ=255)

                                                                                      tGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCgggg                                                               >  1:4004647/1‑150 (MQ=255)

                                                                                       gACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGgt                                                              <  1:8435912/150‑1 (MQ=255)

                                                                                          cGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGtt                                                           <  2:701954/150‑1 (MQ=255)

                                                                                          cGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGtt                                                           >  1:1317105/1‑150 (MQ=255)

                                                                                          cGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGtt                                                           >  2:4166288/1‑150 (MQ=255)

                                                                                           gCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTg                                                          <  1:2744923/150‑1 (MQ=255)

                                                                                           gCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTg                                                          >  2:2246686/1‑150 (MQ=255)

                                                                                           gCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTg                                                          <  1:5827199/150‑1 (MQ=255)

                                                                                           gCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTg                                                          <  2:8424206/150‑1 (MQ=255)

                                                                                           gCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGCGTTg                                                          <  1:4281023/150‑1 (MQ=255)

                                                                                                cagggCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAg                                                     <  1:6934182/147‑1 (MQ=255)

                                                                                                  tGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGgc                                                   <  1:2125978/150‑1 (MQ=255)

                                                                                                     cACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGgccgc                                                <  1:5029206/150‑1 (MQ=255)

                                                                                                     cACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGACTGCATGCTGCCCGCCTCCACCGCACGCTGCAGACAGTACCCGCCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGgccgc                                                >  2:5371196/1‑150 (MQ=255)

                                                                                                      aCTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGgccgcc                                               >  2:3976071/1‑150 (MQ=255)

                                                                                                         gcgcTCGTTCAGCTCCGCAGCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATg                                            <  2:7561482/150‑1 (MQ=255)

                                                                                                         gcgcTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATg                                            <  2:6354514/150‑1 (MQ=255)

                                                                                                           gcTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGt                                          >  2:2701732/1‑150 (MQ=255)

                                                                                                             tCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAgg                                                                            >  2:331879/1‑114 (MQ=255)

                                                                                                             tCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGc                                        >  1:8134413/1‑150 (MQ=255)

                                                                                                             tCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGc                                        >  1:6203286/1‑150 (MQ=255)

                                                                                                                 tCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCt                                    <  2:4024427/150‑1 (MQ=255)

                                                                                                                  cAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTc                                   >  1:4924103/1‑150 (MQ=255)

                                                                                                                    gATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCAt                                 >  2:6000824/1‑150 (MQ=255)

                                                                                                                     aTCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATa                                >  2:1024605/1‑150 (MQ=255)

                                                                                                                       ccTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACa                              >  2:2010703/1‑150 (MQ=255)

                                                                                                                         tCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCggg                                                                <  2:1205819/114‑1 (MQ=255)

                                                                                                                         tCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAg                            <  2:3031773/150‑1 (MQ=255)

                                                                                                                         tCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAg                            >  1:3359034/1‑150 (MQ=255)

                                                                                                                         tCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAg                            >  1:7714397/1‑150 (MQ=255)

                                                                                                                         tCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAg                            >  1:6104313/1‑150 (MQ=255)

                                                                                                                          cATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAgg                           <  1:1617712/150‑1 (MQ=255)

                                                                                                                                cGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAggg                     <  1:8032328/150‑1 (MQ=255)

                                                                                                                                  gcgggcagcaggcagGCCAAGTACAACCTGGCCTGCCTGC‑GCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGgcag                  >  2:8561129/8‑150 (MQ=38)

                                                                                                                                     aTGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGgcagca                >  2:8069264/1‑150 (MQ=255)

                                                                                                                                           tggcaggcCAGTTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGc          <  1:8499956/149‑1 (MQ=255)

                                                                                                                                            ggcaggcCAAGTACAACCTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCa                                                      >  1:2834012/1‑105 (MQ=38)

                                                                                                                                            ggcaggcCAAGTACAACCTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCa                                                      <  2:7277754/105‑1 (MQ=38)

                                                                                                                                             gcaggcCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAg        >  1:7515132/1‑150 (MQ=255)

                                                                                                                                               aggcCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGt      >  1:6704281/1‑150 (MQ=255)

                                                                                                                                                ggcCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGtt     <  2:1399257/150‑1 (MQ=255)

                                                                                                                                                  ccAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTCa   >  1:1050386/1‑150 (MQ=255)

ACCAAACCGGCGTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTCAG  >  NC_002947/5974586‑5974882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`