Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,974,740 T→A 6.8% Y520F (TAC→TTC) ‡ PP_5236 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,974,7400TA 6.8% 978.0 / 39.9 304Y520F (TAC→TTC) ‡PP_5236hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (134/149);  new base A (5/16);  total (139/165)
Fisher's exact test for biased strand distribution p-value = 4.19e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 306 total aligned reads displayed.

ACCGGCGTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTCAGTTCGGC  >  NC_002947/5974591‑5974888
                                                                                                                                                     |                                                                                                                                                    
aCCGGCGTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGt                                                                                                                                                      <  2:1274780/150‑1 (MQ=255)
 ccGGCGTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAAGTTGTa                                                                                                                                                     <  2:35227/150‑1 (MQ=255)
   ggCGTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACt                                                                                                                                                   >  2:7172805/1‑150 (MQ=255)
         aCAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcct                                                                                                                                             <  2:2719713/150‑1 (MQ=255)
          cAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctg                                                                                                                                            >  1:1250702/1‑150 (MQ=255)
           aaaaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctgc                                                                                                                                           <  1:5712101/150‑1 (MQ=255)
           aaaaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGAGCAGGCAGGCCAAGTACAACCTGgcctgc                                                                                                                                           <  1:4045826/150‑1 (MQ=255)
           aaaaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCAGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctgc                                                                                                                                           >  2:3152883/1‑150 (MQ=255)
            aaaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctgcc                                                                                                                                          >  2:563609/1‑150 (MQ=255)
            aaaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctgcc                                                                                                                                          >  2:5475254/1‑150 (MQ=255)
            aaaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctgcc                                                                                                                                          <  2:4951638/150‑1 (MQ=255)
             aaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGgcctgcct                                                                                                                                         <  2:2670720/150‑1 (MQ=255)
             aaTCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAAGTACAACCTGgcctgcct                                                                                                                                         <  2:5503864/150‑1 (MQ=255)
                  ccAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAAGTACAACCTGGCctgcctgcgca                                                                                                                                    <  1:6972290/150‑4 (MQ=39)
                    aGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGccc                                                                                                                                  <  1:684589/150‑1 (MQ=255)
                    aGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGccc                                                                                                                                  <  2:6232150/150‑1 (MQ=255)
                        gCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCt                                                                                                                              >  1:5895537/1‑150 (MQ=255)
                         cAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTc                                                                                                                             >  2:5519438/1‑150 (MQ=255)
                                    acGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCt                                                                                                                  >  1:8133059/1‑150 (MQ=255)
                                     cGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTg                                                                                                                 <  2:5663558/150‑1 (MQ=255)
                                      gCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGc                                                                                                                >  2:1728767/1‑150 (MQ=255)
                                       cATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCa                                                                                                               <  1:3524580/150‑1 (MQ=255)
                                       cATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCa                                                                                                               <  2:3823462/150‑1 (MQ=255)
                                         ttGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGc                                                                                                             <  1:6527201/150‑1 (MQ=255)
                                         ttGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGc                                                                                                             <  2:2276067/150‑1 (MQ=255)
                                           ggTCTGCCCGTTGCCCAGCGCGTCGACACGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCa                                                                                                           <  1:5157159/150‑1 (MQ=255)
                                           ggTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCa                                                                                                           <  1:4176215/150‑1 (MQ=255)
                                           ggTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCa                                                                                                           <  2:7573206/150‑1 (MQ=255)
                                                gCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTAcc                                                                                                      <  1:6658067/150‑1 (MQ=255)
                                                  ccGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCg                                                                                                    >  2:1469852/1‑150 (MQ=255)
                                                   cGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCgg                                                                                                   <  2:8305427/150‑1 (MQ=255)
                                                    gTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGc                                                                                                  >  1:4396075/1‑150 (MQ=255)
                                                         ccAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCt                                                                                             >  2:3345655/1‑150 (MQ=255)
                                                         ccAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCt                                                                                             <  2:5299291/150‑1 (MQ=255)
                                                         ccAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCt                                                                                             >  1:2776575/1‑150 (MQ=255)
                                                         ccAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCt                                                                                             >  1:7220262/1‑150 (MQ=255)
                                                          cAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTc                                                                                            <  1:4825724/150‑1 (MQ=255)
                                                          cAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCATCATCGCCGTCCATGCGCAGGAAGGCAAGTTTGTAATTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTc                                                                                            <  1:2385678/150‑1 (MQ=255)
                                                            gcgcGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGt                                                                                          <  1:1304626/150‑1 (MQ=255)
                                                               cgTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGtgcg                                                                                       <  1:178167/150‑4 (MQ=255)
                                                                   gACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAg                                                                                   <  1:4918759/150‑1 (MQ=255)
                                                                    aCAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAgg                                                                                  >  1:5500541/1‑150 (MQ=255)
                                                                    aCAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAgg                                                                                  >  2:2464841/1‑150 (MQ=255)
                                                                    aCAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAgg                                                                                  >  2:3721191/1‑150 (MQ=255)
                                                                    aCAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGACTGCCTCGTCATCCAgg                                                                                  >  2:7378826/1‑150 (MQ=255)
                                                                      aaGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCTGGTTGTACTTGGCGTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTa                                                                                <  1:4603228/150‑1 (MQ=255)
                                                                       cgCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTaa                                                                               <  1:2682539/149‑1 (MQ=255)
                                                                       aGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTaa                                                                               <  1:3801772/150‑1 (MQ=255)
                                                                        gCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAAt                                                                              >  2:1825477/1‑150 (MQ=255)
                                                                         cAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATg                                                                             >  1:8225410/1‑150 (MQ=255)
                                                                          aGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATgc                                                                            <  2:3984475/150‑1 (MQ=255)
                                                                          aGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATgc                                                                            >  1:2000524/1‑150 (MQ=255)
                                                                                cTGACCGCCAGCTTGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCTGGCAGGCCAGTTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCggg                                                                      <  1:7375215/150‑1 (MQ=255)
                                                                                cTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCggg                                                                      >  1:1376636/1‑150 (MQ=255)
                                                                                cTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCggg                                                                      >  2:6993798/1‑150 (MQ=255)
                                                                                cTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCggg                                                                      <  2:2889406/150‑1 (MQ=255)
                                                                                 tGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCgggg                                                                     >  2:6259013/1‑150 (MQ=255)
                                                                                 tGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCgggg                                                                     >  2:851162/1‑150 (MQ=255)
                                                                                 tGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCgggg                                                                     >  1:4004647/1‑150 (MQ=255)
                                                                                  gACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGgt                                                                    <  1:8435912/150‑1 (MQ=255)
                                                                                    ccGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGgtgt                                                                  <  2:4699997/150‑1 (MQ=255)
                                                                                     cGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGtt                                                                 >  1:1317105/1‑150 (MQ=255)
                                                                                     cGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGtt                                                                 >  2:4166247/1‑150 (MQ=255)
                                                                                      gCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCCCCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTg                                                                <  2:2228161/150‑1 (MQ=255)
                                                                                      gCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTg                                                                >  2:2825019/1‑150 (MQ=255)
                                                                                      gCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTg                                                                <  2:8424206/150‑1 (MQ=255)
                                                                                      gCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTg                                                                <  2:4445197/150‑1 (MQ=255)
                                                                                      gCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGCGTTg                                                                <  1:4281150/150‑1 (MQ=255)
                                                                                          gCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCa                                                            <  1:7981921/150‑1 (MQ=255)
                                                                                             tGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGgc                                                         <  1:2125978/150‑1 (MQ=255)
                                                                                                cACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGgccgc                                                      <  2:259870/150‑1 (MQ=255)
                                                                                                cACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGgccgc                                                      <  1:5029206/150‑1 (MQ=255)
                                                                                                 aCTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGgccgcc                                                     >  2:3786279/1‑150 (MQ=255)
                                                                                                 aCTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGgccgcc                                                     >  2:3976071/1‑150 (MQ=255)
                                                                                                  cTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCa                                                    <  1:3032704/150‑1 (MQ=255)
                                                                                                    gcgcTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATg                                                  <  2:7560840/150‑1 (MQ=255)
                                                                                                        tCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAgg                                                                                  <  1:331879/114‑1 (MQ=255)
                                                                                                           ttCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGc                                           <  1:3251072/150‑1 (MQ=255)
                                                                                                            tCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCt                                          <  2:4024427/150‑1 (MQ=255)
                                                                                                               gATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCAt                                       >  2:6000824/1‑150 (MQ=255)
                                                                                                                aTCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATa                                      >  2:1024605/1‑150 (MQ=255)
                                                                                                                  ccTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACa                                    >  2:2010703/1‑150 (MQ=255)
                                                                                                                   cTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACaa                                   <  2:4432069/150‑1 (MQ=255)
                                                                                                                   cTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACaa                                   <  1:6296372/150‑1 (MQ=255)
                                                                                                                     cATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAgg                                 <  1:1617712/150‑1 (MQ=255)
                                                                                                                        cGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCa                              <  2:3298121/150‑1 (MQ=255)
                                                                                                                            gTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGgc                          >  2:6770036/1‑150 (MQ=255)
                                                                                                                             gcgggcagcaggcagGCCAAGTACAACCTGGCCTGCCTGC‑GCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGgcag                        >  2:8561129/8‑150 (MQ=38)
                                                                                                                               cATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGgcagc                       >  1:8664905/1‑150 (MQ=255)
                                                                                                                                aTGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGgcagca                      >  2:8069101/1‑150 (MQ=255)
                                                                                                                                  gcgcAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCAcc                    >  1:6181696/1‑150 (MQ=255)
                                                                                                                                     caggcaggCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCgg                 <  1:7838740/150‑1 (MQ=255)
                                                                                                                                      tggcaggcCAGTTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGc                <  1:8499956/149‑1 (MQ=255)
                                                                                                                                      aggcaggcCAAGTACAACCTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGc                >  1:7704827/1‑150 (MQ=255)
                                                                                                                                        gcaggcCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAg              >  1:7515132/1‑150 (MQ=255)
                                                                                                                                           ggcCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGtt           <  2:1399257/150‑1 (MQ=255)
                                                                                                                                            gcCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTc          >  2:5607372/1‑150 (MQ=255)
                                                                                                                                             ccAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTCa         >  2:7888767/1‑150 (MQ=255)
                                                                                                                                             ccAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTCa         >  1:1050386/1‑150 (MQ=255)
                                                                                                                                                  ttGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTCAGTTCg    <  1:2284615/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACCGGCGTAACAAAATCCCCAGGTGCAACCGCGCACACGCATTGGTCTGCCCGTTGCCCAGCGCGTCGACAAGCAGGCGCCTGACCGCCAGCATGGCACTGCGCTCGTTCAGATCCTCATCGCCGTCCATGCGCAGGCAGGCCAGGTTGTACTTGGCCTGCCTGCTGCCCGCCTCCACCGCACGCTGCAGCCAGTACCCGGCTGCCTCGTCATCCAGGTAATGCGCCGGGGTGTTGGCCAGGCCGCCATGGTGCAGCTCATACAAGGCCAGGGCAGCACCCGGCAGGTTCAGTTCGGC  >  NC_002947/5974591‑5974888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑