Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,005,983 G→T 9.5% intergenic (+85/+24) PP_5256 → / ← amaA cyclic nucleotide‑binding protein/L‑pipecolate oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,005,9830GT 9.5% 486.9 / 6.5 167intergenic (+85/+24)PP_5256/amaAcyclic nucleotide‑binding protein/L‑pipecolate oxidase
Reads supporting (aligned to +/- strand):  ref base G (90/56);  new base T (6/15);  total (96/71)
Fisher's exact test for biased strand distribution p-value = 8.07e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

CGTTGTTGTTGCAGAAGCCGGCTTCAATCAAGAAGGGTGGCTTCCTGAGTTGGTTGCACAAGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTAAACCCGCTCCTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCATCGGCGAAAACGGAATCGG  >  NC_002947/6005834‑6006120
                                                                                                                                                        |                                                                                                                                         
cggacagcgttctatgagtccggtttttcgctgtaccacgcggggcagtgcctgagggttgtgttgtaagtaccGGCCTCTTCG‑C‑GGGTAAACCCGCTCCTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑ggg                                                                                                                                           >  1:305917‑M1/75‑150 (MQ=255)
cGTTGTTGTTGCAGAAGCCGGCTTCAATCAAGAAGGGTGGCTTCCTGAGTTGGTTGCACAAGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTAAACCCGCTCCTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑ggg                                                                                                                                           <  2:1137616/150‑1 (MQ=255)
cGTTGTTGTTGCAGAAGCCGGCTTCAATCAAGAAGGGTGGCTTCCTGAGTTGGTTGCACAAGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTAAACCCGCTCCTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑ggg                                                                                                                                           <  1:1998918/150‑1 (MQ=255)
cGTTGTTGTTGCAGAAGCCGGCTTCAATCAAGAAGGGTGGCTTCCTGAGTTGGTTGCACAAGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTAAACCCGCTCCTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑ggg                                                                                                                                           <  1:6990812/150‑1 (MQ=255)
 gttgttgttGCAGAAGCCGGCTTCAATCAAGAAGGGTGGCTTCCTGAGTTGGTTGCACAAGTGAGCCTGTGCCGGCCTCTTCGCC‑GGGTAAACCC‑CACCAACAACTAGTGCACCCCCAGAGAGGGCGGTGGTGAACCAGGAGGAGC‑gggg                                                                                                                                          >  1:6391992/1‑149 (MQ=255)
  ttgttgttgCAGAAGCCGGCTTCAATCAAGAAGGGTGGCTTCCTGAGTTGGTTGCACAAGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTAAACCCGCTCCTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGtt                                                                                                                                         <  1:577055/150‑1 (MQ=255)
    gttgttgCAGAAGCCGGCTTCAATCAAGAAGGGTGGCTTCCTGAGTTGGTTGCACAAGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTTAAACCCCGCCAAAAAGTAGTGCCCCCCCGGAGACGGCGGTGGGGGCCCGGGAGGGGC‑GGGGTTa                                                                                                                                       >  1:4334950/1‑150 (MQ=255)
        ttgCAGAAGCCGGCTTCAATCAAGAAGGGTGGCTTCCTGAGTTGGTTGCACAAGTGAGCCAGTGCCGGCCTCTTCG‑C‑GGGTAAACCCGCTCCTACAAGTAGTGCACCGCCGGAGAGGGCGGTGGTGAACCAGTAGGAGC‑GGGTTTACCcg                                                                                                                                   >  2:5057811/1‑150 (MQ=255)
            aGAAGCCGGCTTCAATCAAGAAGGGTGGCTTCCTGAGTTGGTTGCACAAGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTAAACCCGCTCCTACAAGTAGTGCACCGCCAGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGaa                                                                                                                               >  2:2883630/1‑150 (MQ=255)
            aGAAGCCGGCTTCAATCAAGAAGGGTGGCTTCCTGAGTTGGTTGCACAAGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTAAAACCGCGCCTACAAGTAGTGCACCGCCGGAGAGGGCTGTGGTGAACCGGTAGGCGC‑GGGTTTACCcgcgca                                                                                                                               >  2:8405192/1‑148 (MQ=39)
            aGAAGCCGGCTTCAATCAAGAAGGGTGGCTTCCTGAGGTGGTTGCACAAGTGAGCATGTGCCGGCCTCTTCG‑C‑GGGTAAACCCGCTCCTACCAGTAGTGCCCCGCCGGAGAGGGCGGGGGGGAACCGGAAGGAGC‑GGGTTTACCCCCGaa                                                                                                                               >  2:4256230/1‑150 (MQ=38)
              aaGCCGGCTTCAATCAAGACGGGGGGCTTCCTGAGTTGGTGGCACATGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTAAACCCGCTCCTCCAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGACCCTGTAGGAGC‑GGGTTTACCCGCGAaga                                                                                                                             <  2:4598898/150‑1 (MQ=38)
              aaGCCGGCTTCAATCAAGAAGGGTGGCTTCCTGAGTTGGTTGCACAAGTGAGCCTGTGCCGGCCTCTTCG‑CGGGGTAAACCC‑CGCCGACCACTAGTGCACCCCCAGAGAGGGCGGTGGTGAAACAGGGGGGGCGGGGTTTAccccccagg                                                                                                                              >  2:11691/1‑144 (MQ=255)
              aaGCCGGCTTCAATCAAGAAGGGTGGCTTCCTGAGTTGGTTGCACAAGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTAAACCCGCTCCTACAAGTAGTGCGCCGCCTGAGAGGGCTATGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAaga                                                                                                                             >  2:6554127/1‑150 (MQ=255)
               aGCCGGCTTCAATCAAGAAGGGTGGCTTCCTGAGTTGGTTGCACAAGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGGAAACCCCCTCCGACAAGTAGTGCCCCCCCAGAGAGGGCGGTGGTGAACCGGTAGGAGC‑GGGTTTTCCCCCCAagag                                                                                                                            >  1:7329322/1‑150 (MQ=255)
                 ccGGCTTCAATCATGACGGGTGGCTTCATGAGTTGGTTGCACAAGTGAGCGTGTGCGGGCCTCTGCG‑C‑GGTTAAACCCGCTCATACAATTAGTGCACCGCCTGAGTGGGGTGTGGTGAACCTGTCGGAGC‑GGGTTTACCCGCGAAGAGGc                                                                                                                          <  2:7504747/150‑1 (MQ=37)
                 ccGGCTTCAATCACGAAGGGTGGCTTCCTGTGTTGGTTGCAAAAGTGAGCCTGTGCCGGCCTCTTCG‑G‑GGGTAAACCCTCTCCTACAAGTAGTGCACCGCCTGAGTGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGc                                                                                                                          <  2:8002977/150‑1 (MQ=38)
                 ccGGCTTCAATCAAGAAGGGTGGCTTCCTGAGTTGGTTGCACAAGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTAAAACCCCTCCGACAAGTAGTGCACCCCCTGAGAGGGGGGTGGTGAACCGGTAGGAGC‑GGGGTTAACCCCGAAGAGGc                                                                                                                          >  2:6780567/1‑150 (MQ=255)
                   ggCTTCAATCAAGAAGGGTGGCTTCCTGAGTTGGTTGCACAAGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTAAACCCGCTCCTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCa                                                                                                                        <  2:4978903/150‑1 (MQ=255)
                       tcaatcaaGAAGGGTGGCTTCCTGAGTTGGTTGCACAAGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTAAACCCGCGCCTACAAGTAGTTCACCGCCGGGGTGGGCTGTGGTGGACCGGGGGGGGC‑GGGTGGACCCGCGGAGGGGcccctac                                                                                                                    >  2:3164949/1‑145 (MQ=25)
                         aatcaaGAAGGGTGGCTTCCTGAGTTGGTTGCACAAGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTAAAACCGCTCCTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCCCGAAGAGGCCAGTACtt                                                                                                                  >  2:2744718/1‑150 (MQ=255)
                          atcaaGACGGGTGGCTTCCTGTGTTGGTTGCACAAGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTAAACCCGCTCCTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGTTTTACCCGCGAAGAGGCCAGTACTTa                                                                                                                 <  2:157498/150‑1 (MQ=255)
                             aagaagGGTGGCTTCCTGAGTTGGTTGCACAAGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTAAAACCGCTCCTACAAGTAGTGCACCGCCTGGGAGGGCTGTGGTGAACCGGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACATacaa                                                                                                              >  2:3520210/1‑150 (MQ=255)
                               gaagGGTGGCTTCCTGAGTTGGTTGCACAAGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTAAAACCCCTCCGACAAGTAGTGCACCGCCGGGGGGGGCTGTGGGGAACCGGTGGGAGC‑GGGGTTAACCCCGAAGAGGGCCCTACTTacaaca                                                                                                            >  2:5467753/1‑150 (MQ=255)
                               gaagGGTGGCTTCCTGAGTTGGTTGCACAAGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTAAAACCCCTCCGACAAATAGTGCACCCCCGGAGAGGGCTGTGGTGTAACTGTAGGAGA‑GGGTTTAACCCCGAAGAGGCCCCTACTTaaaaca                                                                                                            >  2:5274774/1‑150 (MQ=255)
                                  gggTGGCTTCGTTAGTTGGTTGCACAAGTGAGCCTGTGCGGGCCTCTTCG‑C‑GGGTAAACCCGCTCCTACAAGTAGTGCACCGCCTGAGTGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTacaacacaa                                                                                                         <  2:6067204/150‑1 (MQ=255)
                                      ggCTTCCTGAGTTGGTTGCACAAGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTAAACCCCCTCCTACAAGTAGGGCACCGCCGGCGAGGGCTGTGGGGAACCGGGAGGCGC‑GGGTTTACCCGCGAAGGGGCCAGTACTTACAACACAAccca                                                                                                     >  2:6976236/1‑149 (MQ=37)
                                                gttggttgCACAAGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTTAACCCCCGCCGGCAAGTATTGCACCCCCGGAGAGGGCGGTGGGGGACCGGTAGGAGC‑GGGTTTACCCCCGCAGCGGCCCCTAAATACAAAACAAACCACAGGCACGGc                                                                                           >  2:1494210/1‑150 (MQ=255)
                                                 ttggttgCACAAGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTAAACCCGCTCCTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGTTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGcc                                                                                          <  2:1667797/150‑1 (MQ=255)
                                                        cacaAGTGCGCCTGTGCCGGCCTCTTCG‑C‑GGGTAACCCCGCTCCTACACGTAGTGCACCGCCTGCGTGGGCTGTGGTGACCCTGTAGGCGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTg                                                                                   <  2:7436052/150‑1 (MQ=38)
                                                            aGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTAAAACCCCTCCAAAAAGTAGTGCACCCCCCGAGGGGGCTGTGGTGAAACGGTGGGGGC‑GGGTTTAACCCCCAAGAGGCCAGTTCATAAAAAAAAACCCCCAGCCCCAGCCCCCCGGGGTAc                                                                               >  2:5250941/1‑150 (MQ=255)
                                                              tGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTAAAACCCCAACAACAAATAGTTCACCCCCGGAGAGGGCGGTGGTGTACCATTTGGAGC‑GGGTTTACCCCCCAAGAGGCCCCTAAATAAAACACAAACCACACGCCCAGCCCCCCGGGGTACac                                                                             >  2:4117570/1‑149 (MQ=255)
                                                              ggcgCCTGGGCCGGCCTCTGCG‑G‑GGGCAACCCCGCCCCACCACGGAGGGCCCCGCTTGAGCGGCCTGTGGTCACCCTGTTGTCGG‑GGTTTTCCCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAg                                                                             <  1:7630735/147‑1 (MQ=255)
                                                               gcgCCGGTGCCGGCCTCGTCG‑C‑GGGAAACCCCGCTCAACCATTTTGTCCCCCGCCTGCGTGGGTTGTGGTGACCGTGTCGGAGG‑GGTTTAACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGc                                                                            <  1:8379140/148‑1 (MQ=255)
                                                               gAGCCTGTGCCGGCCTCTTCG‑C‑GGGTAAACCCGCTCCTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGc                                                                            <  2:8539305/150‑1 (MQ=255)
                                                                     gtgCCGGCCTCTTCG‑G‑GGGTAAACCCGCTCCTACAAGTCGTGCACCGCCTGAGAGGGCTGTGGTGAACCTTTAGGAGC‑GGTTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGaaaaa                                                                      <  2:313579/150‑1 (MQ=255)
                                                                       gCCGGCCTCTTCG‑C‑GGGTAAACCCGCTCCTACACGTCGTGCACCGCCTGAGCGGGCTGTGGTGACCGTGTTGGCGG‑GGTTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAAcc                                                                    <  1:575578/150‑1 (MQ=38)
                                                                         cGGCGTCTTCG‑C‑TGGTAAACCCTCTCCTACAAGTAGTGAACGTCCTTTGAGGGTTTTGGTGAACCTGTTGTTGC‑GGTTTTACCCTCGAAGATTCCAGTAATTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGAGaatatcagg                                                                  <  2:2663699/150‑8 (MQ=255)
                                                                         cGGCCTCTTCG‑C‑GGGTAAACCCGCTCCTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCgg                                                                  >  2:2056466/1‑150 (MQ=255)
                                                                          ggCCTCTTGG‑C‑GGGAAAACCCGCCCACACACGTCGTGCACCGCCTGCGCGGGGTGTGTTTACCGTTTTGGTGG‑GGTTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGa                                                                 <  1:8181713/150‑1 (MQ=255)
                                                                          gcccTCTGGG‑C‑GGGAAACCCCGATCATACACTTAGTGCACCGCGTGTGTGGGGTGTGGTCAACCTTTTGGGGG‑GGTTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGa                                                                 <  1:6619351/148‑1 (MQ=255)
                                                                          GGCCTCTTCG‑G‑GGGTAACCCGGCTCCTCCACGTCGGGCACCGCGTGTGCGGGCTGTGGTGAACCTGTTGGCGG‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGa                                                                 >  1:7804738‑M1/1‑150 (MQ=255)
                                                                          GGCCTCTTCG‑C‑GGGTAAACCCGCTCCTACACGTAGTGCACCGCCTGTGAGGGCTGTGGTGAACCTGTTGGAGC‑GGTTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGa                                                                 >  1:688615‑M1/1‑150 (MQ=255)
                                                                          GGCCTCTTCG‑C‑GGGTAAACCCGCTCCTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGa                                                                 >  2:2028244‑M1/1‑150 (MQ=255)
                                                                          GGCATCTTCG‑C‑GGGAAACCCCGCTCCTAAAAGTTGTGCACCGCCTGTGTGGGCTTTGGTGAACGTGTTGGTGG‑GGTTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGa                                                                 >  1:5356751‑M1/1‑150 (MQ=255)
                                                                            ccTCTTCG‑C‑GGGTAAACCCCCGCCAACAACTAGTGCCCCCCCGGAGAGGGCGGTGGGGGACCAGTAGGAGC‑GGGTTTACCCCCGAAGACGCCCCTACATACAACACCACCCACAGGCCCAGCCCCCCCGGGTACAGCGCAAAAcccgcca                                                               >  2:844661/1‑145 (MQ=255)
                                                                              TCTTCG‑C‑GGGTAAACCCGCTCCTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCa                                                             >  2:4782338‑M1/1‑150 (MQ=255)
                                                                               CTTCG‑C‑GGGTAAACCCGCTCCTACAAGTAGTGCACCGCCTTAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCAt                                                            >  2:4167852‑M1/1‑150 (MQ=255)
                                                                               CTTCG‑C‑GGGTAAACCCGCTCCTACAAGTAGTGCACCGCCTGAGAGGGCTGTGTTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCAt                                                            >  2:4168011‑M1/1‑150 (MQ=255)
                                                                                TTCG‑C‑GGGTAAACCGGCTCCTACATGTAGTGCACCGCCTGTGTGGGCTGTGGTGAACCTGTAGGAGG‑GGTTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATa                                                           >  1:2827320‑M1/1‑150 (MQ=255)
                                                                                     C‑GGGTAAACCCGCTCCTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAAc                                                       <  1:4961192‑M1/150‑1 (MQ=255)
                                                                                     C‑GGGTAAACCCGCTCCTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAAc                                                       <  1:2125198‑M1/150‑1 (MQ=255)
                                                                                       gtgaaaccccGATCCTACATTTTGTCCACCGCGTGTGTGGGGTGTTGTTAACGTGTTGGCGG‑GGTTTAACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACg                                                      <  1:2443413/143‑1 (MQ=255)
                                                                                         GTAAACCCGCTCCTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCt                                                    >  2:2266472‑M1/1‑150 (MQ=255)
                                                                                         GTAAACCCGCTCCTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCt                                                    <  2:1576279‑M1/150‑1 (MQ=255)
                                                                                         GTAAACCCGCTCCTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCt                                                    <  1:877857‑M1/150‑1 (MQ=255)
                                                                                               CCGCTCCTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGc                                              <  2:4956140‑M1/150‑1 (MQ=255)
                                                                                               CCGCTCCTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGc                                              <  1:8335121‑M1/150‑1 (MQ=255)
                                                                                                     CTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCgac                                        <  2:6049363‑M1/150‑2 (MQ=255)
                                                                                                     CTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGaa                                        <  1:1249141‑M1/150‑1 (MQ=255)
                                                                                                      TACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAg                                       <  2:3591598‑M1/150‑1 (MQ=255)
                                                                                                      TACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAg                                       <  1:3797380‑M1/150‑1 (MQ=255)
                                                                                                      TACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAg                                       <  1:7616264‑M1/150‑1 (MQ=255)
                                                                                                        cccgtcgtGCACCGCCTGAGAGGGCTGTGGTGCCCCTGTAGGAGC‑GGGTGACCCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGgc                                     <  2:8215641/144‑1 (MQ=255)
                                                                                                         AAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGgcg                                    <  2:1396227‑M1/150‑1 (MQ=255)
                                                                                                           gtagtGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGgcgcg                                  >  1:2377173/1‑150 (MQ=255)
                                                                                                           gtagtGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGgcgcg                                  <  2:4234554/150‑1 (MQ=255)
                                                                                                           gtagtGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGgcgcg                                  >  1:4373559/1‑150 (MQ=255)
                                                                                                            tagtGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAGAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGgcgcgc                                 >  1:1457923/1‑150 (MQ=255)
                                                                                                             agtGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCt                                <  2:5760820/150‑1 (MQ=255)
                                                                                                               tGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTca                              >  2:7589772/1‑150 (MQ=255)
                                                                                                                gCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTcac                             >  2:3769964/1‑150 (MQ=255)
                                                                                                                gCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTcac                             >  1:8692445/1‑150 (MQ=255)
                                                                                                                  aCCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCacac                           >  2:8704666/1‑150 (MQ=255)
                                                                                                                     gCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGc                        <  2:8335890/150‑1 (MQ=255)
                                                                                                                      ccTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCt                       >  2:2955854/1‑150 (MQ=255)
                                                                                                                       cTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTc                      >  1:5364651/1‑150 (MQ=255)
                                                                                                                        tGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCa                     >  2:2731072/1‑150 (MQ=255)
                                                                                                                         gagaGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCAt                    >  2:4836629/1‑150 (MQ=255)
                                                                                                                         gagaGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCAt                    >  1:3516851/1‑150 (MQ=255)
                                                                                                                          agagGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCATc                   >  2:3767335/1‑150 (MQ=255)
                                                                                                                           gagGGCTGTGGTGAACGTGTTGGTGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCATCg                  <  1:5088847/150‑1 (MQ=255)
                                                                                                                            agGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCATCgg                 >  1:6409332/1‑150 (MQ=255)
                                                                                                                              gggtggggtgAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCATCGGCg               <  1:7109807/144‑1 (MQ=255)
                                                                                                                              ggCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCATCGGCg               >  1:2326373/1‑150 (MQ=255)
                                                                                                                                 tgtgGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCATCGGCGaaa            <  2:1798226/150‑1 (MQ=255)
                                                                                                                                 tgtgGTGAACCTGTAGGAGA‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCATCGGCGaaa            >  2:6627429/1‑150 (MQ=255)
                                                                                                                                  ggggtcAACCTGTAGGAGG‑GGTTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCATCGGCGaaaa           <  1:1843596/148‑1 (MQ=255)
                                                                                                                                   tggtgAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCATCGGCGAAAAc          <  2:6391032/150‑1 (MQ=255)
                                                                                                                                   tggtgAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCATCGGCGAAAAc          <  2:7908561/150‑1 (MQ=255)
                                                                                                                                   tggtgAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCATCGGCGAAAAc          <  2:4258936/150‑1 (MQ=255)
                                                                                                                                     gtgAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCATCGGCGAAAACgg        >  1:7312523/1‑150 (MQ=255)
                                                                                                                                     gtgAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCATCGGCGAAAACgg        >  2:7822558/1‑150 (MQ=255)
                                                                                                                                       gAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCATCGGCGAAAACGGaa      >  1:3435453/1‑150 (MQ=255)
                                                                                                                                           cTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCGGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCATCGGCGAAAACGGAATCgc  >  2:7507594/1‑149 (MQ=255)
                                                                                                                                           cTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCATCGGCGAAAACGGAATCgg  >  1:1319576/1‑150 (MQ=255)
                                                                                                                                           cTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCATCGGCGAAAACGGAATCgg  >  1:1367894/1‑150 (MQ=255)
                                                                                                                                           cTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCATCGGCGAAAACGGAATCgg  >  1:3594124/1‑150 (MQ=255)
                                                                                                                                           cTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCATCGGCGAAAACGGAATCgg  >  2:4171352/1‑150 (MQ=255)
                                                                                                                                           cTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCATCGGCGAAAACGGAATCgg  >  2:516826/1‑150 (MQ=255)
                                                                                                                                                        |                                                                                                                                         
CGTTGTTGTTGCAGAAGCCGGCTTCAATCAAGAAGGGTGGCTTCCTGAGTTGGTTGCACAAGTGAGCCTGTGCCGGCCTCTTCG‑C‑GGGTAAACCCGCTCCTACAAGTAGTGCACCGCCTGAGAGGGCTGTGGTGAACCTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGTACTTACAACACAACCCTCAGGCACTGCCCCGCGTGGTACAGCGAAAAACCGGACTCATAGAACGCTGTCCGCAGCGAAGGCGCGCTCACACCGCTCATCGGCGAAAACGGAATCGG  >  NC_002947/6005834‑6006120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑