BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,032,228	A→T	5.6%	F212I (TTC→ATC) ‡	PP_5277 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,032,228	0	A	T	5.6%	997.6 / 32.9	303	F212I (TTC→ATC) ‡	PP_5277	MFS transporter
Reads supporting (aligned to +/- strand): ref base A (146/138); new base T (11/6); total (158/145)
Fisher's exact test for biased strand distribution p-value = 3.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.94e-01

Only 100 of 307 total aligned reads displayed.

TCGCGACATATGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTC  >  NC_002947/6032079‑6032376

   cgACATATGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGa                                                                                                                                                   <  1:3725496/150‑1 (MQ=255)

      cATATGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGc                                                                                                                                                <  2:646398/150‑1 (MQ=255)

       atatGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCg                                                                                                                                               >  2:2847126/1‑150 (MQ=255)

        tatGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGTTCGAGGCGa                                                                                                                                              <  1:2797518/150‑1 (MQ=255)

        tatGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGa                                                                                                                                              >  1:4223422/1‑150 (MQ=255)

         atGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGTTCGAGGCGat                                                                                                                                             <  1:2077115/150‑2 (MQ=255)

           ggTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCCATACACCGGCAGCACGGTGAGGATCATCGCCTCGACCGAGGCGAAcc                                                                                                                                           >  2:1054939/1‑149 (MQ=255)

           ggTCGGCCAGCGCGCCGATCGGCAATAGCAGCACCGCATAACCCACCACCACGGTACTCACCATGAACAAGGCGATATCGGTGCTGAAGCCCTGCAGCAGGCAATACACCCGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACa                                                                                                                                           >  2:567681/1‑150 (MQ=255)

            gTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAg                                                                                                                                          >  1:1819981/1‑150 (MQ=255)

                    gcgcgcCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATgg                                                                                                                                  >  2:6656067/1‑150 (MQ=255)

                      gcgcCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGcc                                                                                                                                <  2:7700224/150‑1 (MQ=255)

                       cgcCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGccc                                                                                                                               <  1:132181/150‑1 (MQ=255)

                        gcCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCa                                                                                                                              >  1:874989/1‑150 (MQ=255)

                         ccGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAg                                                                                                                             <  1:1012479/150‑1 (MQ=255)

                           gATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGc                                                                                                                           <  2:6512648/150‑1 (MQ=255)

                                   aTTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCg                                                                                                                   <  1:5165762/150‑1 (MQ=255)

                                       cagcaCCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCaa                                                                                                               <  2:4185867/150‑1 (MQ=255)

                                        agcaCCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAg                                                                                                              <  1:1170499/150‑1 (MQ=255)

                                        agcaCCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAg                                                                                                              <  1:3188415/150‑1 (MQ=255)

                                              gCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCTGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGTCAATTACCGGCAAGCGTCgg                                                                                                        <  1:2556202/150‑1 (MQ=255)

                                              gCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCgg                                                                                                        <  1:6998930/150‑1 (MQ=255)

                                              gCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCgg                                                                                                        <  1:16842/150‑1 (MQ=255)

                                              gCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCgg                                                                                                        <  1:8096724/150‑1 (MQ=255)

                                                aTCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCa                                                                                                      <  2:3063683/150‑1 (MQ=255)

                                                 tCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAg                                                                                                     >  2:7437891/1‑150 (MQ=255)

                                                 tCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAg                                                                                                     >  1:6471453/1‑150 (MQ=255)

                                                  cacccaccACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGa                                                                                                    >  2:2479896/1‑150 (MQ=255)

                                                   ccccaccACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGaa                                                                                                   <  2:6173882/149‑1 (MQ=255)

                                                   acccaccACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGaa                                                                                                   <  1:5360835/150‑1 (MQ=255)

                                                    cccaccACCACGGTGCTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAg                                                                                                  <  1:7269341/150‑1 (MQ=255)

                                                    cccaccACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAg                                                                                                  >  1:7927439/1‑150 (MQ=255)

                                                    cccaccACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAg                                                                                                  <  2:2174567/150‑1 (MQ=255)

                                                     ccaccaccaCGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAgg                                                                                                 >  2:7747691/1‑150 (MQ=255)

                                                       accaccacGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCg                                                                                               >  2:2266847/1‑150 (MQ=255)

                                                         caccacGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGaa                                                                                             <  2:4105747/150‑1 (MQ=255)

                                                          accacGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAAc                                                                                            <  1:3918937/150‑1 (MQ=255)

                                                            cacGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAg                                                                                          >  1:4861544/1‑150 (MQ=255)

                                                                 tACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGc                                                                                     <  1:5853157/150‑1 (MQ=255)

                                                                 tACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGc                                                                                     <  2:5867912/150‑1 (MQ=255)

                                                                    tCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCaa                                                                                  >  1:5497621/1‑150 (MQ=255)

                                                                    tCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCaa                                                                                  >  1:3652245/1‑150 (MQ=255)

                                                                      aCCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAg                                                                                <  2:2971664/150‑1 (MQ=255)

                                                                      aCCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAg                                                                                <  1:6606569/150‑1 (MQ=255)

                                                                      aCCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAg                                                                                >  2:8163789/1‑150 (MQ=255)

                                                                      aCCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAg                                                                                >  2:4589508/1‑150 (MQ=255)

                                                                         aTGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTg                                                                             >  1:7468166/1‑150 (MQ=255)

                                                                         aTGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTg                                                                             >  2:60973/1‑150 (MQ=255)

                                                                          tGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGa                                                                            <  2:6975018/150‑1 (MQ=255)

                                                                             aCAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGacac                                                                         >  2:1992172/1‑150 (MQ=255)

                                                                                 ggCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCa                                                                     <  2:5144853/150‑1 (MQ=255)

                                                                                    gATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGc                                                                  >  1:669378/1‑150 (MQ=255)

                                                                                    gATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGc                                                                  <  2:7659168/150‑1 (MQ=255)

                                                                                             gCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCt                                                         >  1:4995102/1‑150 (MQ=255)

                                                                                              cTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTc                                                        <  1:2369279/150‑1 (MQ=255)

                                                                                                gAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCACTTACCGGCCAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCTGGCTTCCGTGCTCgg                                                      >  1:7363591/1‑150 (MQ=255)

                                                                                                gAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCgg                                                      >  1:4907944/1‑150 (MQ=255)

                                                                                                gAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCgg                                                      >  2:7818941/1‑150 (MQ=255)

                                                                                                 aaGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGgc                                                     >  2:6860352/1‑150 (MQ=255)

                                                                                                    cccTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCt                                                  >  2:8247399/1‑150 (MQ=255)

                                                                                                     ccTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGc                                                                                                                                 >  2:2712706/1‑70 (MQ=255)

                                                                                                     ccTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTc                                                 <  1:4463607/150‑1 (MQ=255)

                                                                                                      ctgctgCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCACTGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTcc                                                >  2:8453799/1‑150 (MQ=255)

                                                                                                      ctgctgCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTcc                                                <  2:4883031/150‑1 (MQ=255)

                                                                                                       tgctgcAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTcca                                               >  1:2267293/1‑150 (MQ=255)

                                                                                                            cAGGCAATACACCCGCAGCAAGGTGAGGATCATCGCCACGAACGACGCCAACAGCGCACAGGCCCAGCCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGACGCCAAAGGTGCCACAGCAGGCTGCCGAGCTCGGCGCGCCACCACg                                          >  2:605417/1‑150 (MQ=255)

                                                                                                             aGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCcaccactc                                         <  2:8109634/150‑3 (MQ=255)

                                                                                                             aGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGc                                         >  1:7701141/1‑150 (MQ=255)

                                                                                                                 aaTACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCca                                     <  1:2926598/150‑1 (MQ=255)

                                                                                                                   tACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCcagc                                   <  2:1115457/150‑1 (MQ=255)

                                                                                                                      acCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGa                                >  2:3260527/1‑150 (MQ=255)

                                                                                                                      acCGGCAGCAAGGTGAGGAACATCGCCTCGAACGAGGCGAACAGGGCAAAGGCCCAGGCAATAACCGCCAAGCGTCGGCACAAGGCGAACAGGACGCCAAAGGTGACACTGCACGCTTCCGTGCTCGGCGCTCCACCACCCCCCAGCAGa                                >  2:1882129/1‑150 (MQ=255)

                                                                                                                       ccGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGaa                               >  1:1202029/1‑150 (MQ=255)

                                                                                                                       ccGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGaa                               >  2:3535006/1‑150 (MQ=255)

                                                                                                                       ccGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGaa                               <  1:6810706/150‑1 (MQ=255)

                                                                                                                         ggCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAAcc                             <  1:67219/150‑1 (MQ=255)

                                                                                                                            agcaAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAgc                          >  1:185430/1‑150 (MQ=255)

                                                                                                                               aaGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAgcggc                       >  1:6094001/1‑150 (MQ=255)

                                                                                                                               aaGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAgcggc                       <  1:8483812/150‑1 (MQ=255)

                                                                                                                                  gTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAgcggcgcg                    <  1:6412828/150‑1 (MQ=255)

                                                                                                                                       gATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCcag               <  2:7077528/150‑1 (MQ=255)

                                                                                                                                       gATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCcag               <  1:4006055/150‑1 (MQ=255)

                                                                                                                                       gATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCcag               >  1:3463776/1‑150 (MQ=255)

                                                                                                                                       cctgttcGCCTCGTTCGAGGCGATCAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCcag               >  2:3722411/6‑150 (MQ=255)

                                                                                                                                       cctgttcGCCTCGTTCGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCcag               >  2:3420811/6‑150 (MQ=255)

                                                                                                                                        ctgttcGCCTCGTTCGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCcagc              >  1:8223850/5‑150 (MQ=255)

                                                                                                                                         tgttcGCCTCGTTCGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCcagca             >  1:753908/4‑150 (MQ=255)

                                                                                                                                         tgttcGCCTCGTTCGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCcagca             >  1:4635154/4‑150 (MQ=255)

                                                                                                                                         tcatcGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCcagca             >  2:7191918/1‑150 (MQ=255)

                                                                                                                                         tcatcGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCcaaca             >  2:8022542/1‑150 (MQ=255)

                                                                                                                                          gttcGCCTCGTTCGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCcagcag            >  1:593864/3‑150 (MQ=255)

                                                                                                                                            tcGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCcagcagca          <  2:5989239/150‑1 (MQ=255)

                                                                                                                                            tcGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCcagcagca          <  2:3317944/150‑1 (MQ=255)

                                                                                                                                            tcGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCcagcagca          <  2:4223751/150‑1 (MQ=255)

                                                                                                                                            tcGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCcagcagca          >  1:7166267/1‑150 (MQ=255)

                                                                                                                                            tcGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCcagcagca          >  1:7941373/1‑150 (MQ=255)

                                                                                                                                               ccTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGc       >  2:1417582/1‑150 (MQ=255)

                                                                                                                                                 tCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCg     <  2:4747505/150‑1 (MQ=255)

                                                                                                                                                 tCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCg     >  2:6712681/1‑150 (MQ=255)

                                                                                                                                                 tCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCg     <  2:3160946/150‑1 (MQ=255)

                                                                                                                                                  cGACCGAGGCGAACCGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCgg    <  2:1796491/150‑1 (MQ=255)

TCGCGACATATGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAACGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTC  >  NC_002947/6032079‑6032376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`