BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	524,177	T→G	6.1%	intergenic (+41/‑775)	tyrS → / → PP_16SC	tyrosine‑‑tRNA ligase/16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	524,177	0	T	G	6.1%	633.2 / 4.0	215	intergenic (+41/‑775)	tyrS/PP_16SC	tyrosine‑‑tRNA ligase/16S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base T (49/145); new base G (16/2); total (66/149)
Fisher's exact test for biased strand distribution p-value = 1.30e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 217 total aligned reads displayed.

GTTGATGGTGCAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATT  >  NC_002947/524029‑524326

 ttGATGGTGCAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAg                                                                                                                                                      <  2:1704022/150‑1 (MQ=255)

   gATGGTGCAGTGGTCGACAATGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGc                                                                                                                                                    <  2:3140314/150‑1 (MQ=255)

   gATGGTGCAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGGGG‑CCGG‑TAGGc                                                                                                                                                    >  2:7820840/1‑150 (MQ=255)

   gATGGTGCAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGc                                                                                                                                                    <  1:5078243/150‑1 (MQ=255)

     tGGTGCAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGGGG‑GCGG‑Tggggca                                                                                                                                                  >  1:7059563/1‑147 (MQ=255)

     tGGTGCAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTGTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCCG‑TAGGcca                                                                                                                                                  >  2:8750061/1‑149 (MQ=255)

        tGCAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTccc                                                                                                                                               <  1:3123440/150‑1 (MQ=255)

        tGCAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCAccc                                                                                                                                               >  2:5725998/1‑150 (MQ=255)

         gCAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTcccc                                                                                                                                              <  1:6311006/150‑1 (MQ=255)

           aGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCTA‑CCGGCCTCCCCGc                                                                                                                                            <  1:1873713/150‑1 (MQ=255)

             tGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGTTTACCCTCAAGGCTGAGTGATTGCCTGGTTGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCTA‑CCGGCCTCCCCGC‑tt                                                                                                                                         <  1:7326790/150‑1 (MQ=255)

              ggTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAGGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATAGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑ttt                                                                                                                                        <  2:4147048/150‑1 (MQ=255)

               gTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGTGG‑CCGG‑TAGGCCGACCCGC‑attt                                                                                                                                       >  1:8040716/1‑149 (MQ=255)

                   aCAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGGGG‑GCCG‑GAGGGCACCCCCC‑CGTGtttt                                                                                                                                   >  1:1984506/1‑150 (MQ=255)

                    cAAGGGCTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CGCG‑TAGGCCACCCCCC‑GGTGttttt                                                                                                                                  >  2:8212156/1‑150 (MQ=255)

                     aaGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGTGG‑CCGG‑TAGGCCTCCCCCC‑CTTGtttttt                                                                                                                                 >  2:1534495/1‑149 (MQ=255)

                     aaGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGGGG‑CCGG‑GAGGCCTCCCCCC‑GGTTTTTTTg                                                                                                                                 >  2:1863629/1‑150 (MQ=255)

                          cTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCCAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGtt                                                                                                                            <  2:5052173/150‑1 (MQ=255)

                           ttCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTCGCTATATAGAAGC‑GGGGCGG‑CCGG‑GGGGCCACCCCGC‑GTGGtttttttgggtt                                                                                                                           >  2:8717121/1‑143 (MQ=255)

                           ttCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGGGG‑CCCG‑GAGTCCAACCCCC‑GTTGTGTTTGGGGgtt                                                                                                                           >  1:1291410/1‑150 (MQ=255)

                            tCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑GCGG‑TATGCCAAACCCC‑GGTGTTTTTTGGGtttt                                                                                                                          >  2:808094/1‑149 (MQ=255)

                             cATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTTGGGTTtgg                                                                                                                         >  2:4874844/1‑150 (MQ=255)

                             attGTTTGTCCTTGGTGCTACCCTTGTGTGCCAAGCGGGAAAGAAGTCGTTTGGCGGGGTTACCCTCACGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGACGG‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTtgg                                                                                                                         <  1:2553403/148‑1 (MQ=255)

                              aTGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCTTTTGGCCGGGTTACCCTCACGGCTGAGTGATTGCCTGTTTGGTGTAGCTATATCGAAGG‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTtggt                                                                                                                        <  1:5225846/150‑1 (MQ=39)

                              aTGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTtggt                                                                                                                        <  1:1435410/150‑1 (MQ=255)

                               tGTTTGTCCTTGGTGCTACCCTTGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCACGGCTGAGTGATTGCCTGGATGGTGTAGCTATATCGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTtggtg                                                                                                                       <  1:4893448/150‑1 (MQ=255)

                                 tttGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTggtggt                                                                                                                     <  2:6818264/150‑1 (MQ=255)

                                    gTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGGGG‑CCGG‑TAGGCCACCCCCCAGTGGTTTTTGGGGTTTGG‑GGTTgg                                                                                                                  >  2:5237196/1‑149 (MQ=255)

                                        ttGGTGCTACCCTTGTGTGCCAAGGGGGCATTAAGTCGTTTGGCCGGGTTACCCTCCCGGCTGCGTGTTTGCCTGGTTGGTGTAGCTATATCGAAGG‑GGGGAGG‑CGGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCtgtt                                                                                                              <  1:3799910/150‑1 (MQ=37)

                                        ttGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGCGGGGGGGG‑CGGG‑GGGGCACCCCCCC‑GTGGTTTTTgggggttgggggtttgggg                                                                                                               >  1:980391/1‑145 (MQ=255)

                                        ttGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGGGG‑GCGG‑TAGGGCACCCCCC‑GTTGGTTTTGGGGGTTggtgggtgggggt                                                                                                              >  1:7882512/1‑145 (MQ=255)

                                        ttGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGGGG‑CCGGTTTGCCCCCCCCCC‑GTTGTTTTTGGGG‑TTGGTGGTTTGtgtt                                                                                                              >  1:6972377/1‑150 (MQ=255)

                                         tGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCtgttg                                                                                                             <  2:482363/150‑1 (MQ=255)

                                          ggTGCTACCCCTGTGTGCCAAGCGGGCAATAAGTCGTTTGGCGGGGTTACCCTCAAGGCTGTGTGTTTGCCTGGTTGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TTGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCtgttgt                                                                                                            <  1:5302142/150‑1 (MQ=38)

                                           gtgcgccccATGTGGGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATGGCCTGGAGGGTGTAGCTATATAGACGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTGGGGGGTTGCTGTTGTa                                                                                                           <  2:5748606/144‑1 (MQ=38)

                                              cTACCCATGTGTGCCACGCGGGCAATAAGTCGTTTGGCCGGGTTACCCTAATGGGTGTGTGATTGCCTGGATGGTGTAGATATATAGCCGG‑GGGGGGG‑CCGG‑TCGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTaggg                                                                                                        <  1:439069/150‑1 (MQ=37)

                                                  ccATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGGGG‑CCGG‑TATGCCGCCCCGCGGTGGTTTTTGGGGTTT‑GTGGTTGGTGGTGTtggggggg                                                                                                    >  2:6891945/1‑148 (MQ=255)

                                                   cATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTagggggagg                                                                                                   <  2:5973883/150‑1 (MQ=255)

                                                     tgtgtgCCAAGCGGTCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTagggggagggg                                                                                                 <  2:7301081/150‑1 (MQ=255)

                                                      gtgtgCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTagggggaggggg                                                                                                <  2:1135222/150‑1 (MQ=255)

                                                        gtgCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCt                                                                                              <  2:110822/150‑1 (MQ=255)

                                                          gCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGt                                                                                            <  2:1358590/150‑1 (MQ=255)

                                                          gCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGt                                                                                            <  2:1387173/150‑1 (MQ=255)

                                                             aaGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGGGG‑CCGG‑GTGGCCGCCCCCC‑CGTGTTTTTTGGGGTTGGTGGTTGGGGTTGGtgggggtgggggggggttt                                                                                         >  1:4300861/1‑143 (MQ=255)

                                                             aaGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGtttt                                                                                         <  2:447748/150‑1 (MQ=255)

                                                             aaGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGtttt                                                                                         <  2:2092529/150‑1 (MQ=255)

                                                             aaGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGtttt                                                                                         <  1:2727175/150‑1 (MQ=255)

                                                             aaGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGtttt                                                                                         <  2:7981459/150‑1 (MQ=255)

                                                                cGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCCGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTgag                                                                                      <  2:4996669/150‑1 (MQ=255)

                                                                 gggCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTgaga                                                                                     <  2:3408852/150‑1 (MQ=255)

                                                                 gggCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTgaga                                                                                     <  2:1020448/150‑1 (MQ=255)

                                                                   gCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGGGGCCCGG‑TTGGCAACCCCCC‑GTGTTTTTTGGGGTTGGGGGGTTGGGGTT‑TATGGGGGGGGGGGGGtttttttagg                                                                                   >  1:701609/1‑144 (MQ=255)

                                                                   gCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAgg                                                                                   <  2:1623268/150‑1 (MQ=255)

                                                                   gCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAgg                                                                                   <  2:7800979/150‑1 (MQ=255)

                                                                     aagaagTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGCGGGGGGGG‑CGGG‑TGGGCGCCCCCCC‑GTGGTTTTTTGGGTTT‑GTGGTTGGTGTTGGTTGGGGTGGGGGGGGGTTTGTGAGGgt                                                                                 >  2:2923432/1‑150 (MQ=255)

                                                                        atgTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTATCTATATAGAAGC‑GGGGAGG‑CCGG‑TTGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGtt                                                                              <  2:3664825/148‑1 (MQ=255)

                                                                             gTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCgg                                                                         <  2:3841309/150‑1 (MQ=255)

                                                                              tttGGCCGGGTTACCCTCAAGGCTGAGTGTTTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGt                                                                        <  1:8617532/150‑1 (MQ=255)

                                                                              tttGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGt                                                                        <  2:2440814/150‑1 (MQ=255)

                                                                              tttGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGt                                                                        <  2:5767493/150‑1 (MQ=255)

                                                                                tGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGACGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTga                                                                      <  2:5415731/150‑1 (MQ=255)

                                                                                gggCCGGGTTACCCTCATGGCTGTGTGATGGCCGGGCTGGTGTAGCTATATCGACGG‑GGGGCGG‑CGGG‑TCGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCGGTTTTGAGAGGGTGTTGGCGGTga                                                                      <  1:2648824/149‑1 (MQ=37)

                                                                                   cggtgTTACCCTCATGGCTTTTTTATTGCGTGGAGGGTTTAGCTATTTAGATGG‑GGGGAGG‑CGGT‑TTGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGAt                                                                   <  1:3174021/146‑1 (MQ=21)

                                                                                   ccGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGAt                                                                   <  2:4403272/150‑1 (MQ=255)

                                                                                   ccGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGAt                                                                   <  2:8647889/150‑1 (MQ=255)

                                                                                         tACCCTCACGGCTGTGTGATTGCCTGGTTGGTGTTGCTATATAGATGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTcc                                                             <  1:4549678/150‑1 (MQ=37)

                                                                                          aCCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGATG‑CCCC‑TAGGCCACACACC‑GTTGTTTTTGTGGTTTTGTGGTTTGTTGTGTATGGGGGGGGGGGGGTTTTGATAGGGTGTTTGCGGTGAGATTTGTGCg                                                            >  2:6390655/1‑150 (MQ=255)

                                                                                          aCCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCg                                                            <  2:7071694/150‑1 (MQ=255)

                                                                                             cTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGGGG‑CCGG‑TAGGCCTCCCCGC‑GTTGTTTTTGGGGGTTGGGTGGGGGGGGTGTGGGGGGGGGGGGCGGGTTTGTGGGGGGGTTGTGGGTGGGTTTTTTCCGccc                                                         >  2:4589497/1‑149 (MQ=255)

                                                                                                aaGGCTGAGTGATTGCCTGGTTGGTTTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACaa                                                      <  1:2803681/150‑1 (MQ=255)

                                                                                                      gAGTGATTGCCTGGATGGTGTAGCTATATAGACGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTg                                                <  2:2514633/150‑1 (MQ=255)

                                                                                                        gtgtTTGCCTGGATGGTGTAGCTATATCGAAGC‑GGGGTGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCa                                              <  1:4513069/150‑1 (MQ=255)

                                                                                                        gTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCa                                              <  1:6460292/150‑1 (MQ=255)

                                                                                                              gCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCt                                        <  2:6180869/150‑1 (MQ=255)

                                                                                                                 tggttggtGTAGCTATATAGACGC‑GGGGAGG‑CCGT‑TAGGCCCCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCtgtg                                     <  1:745769/150‑1 (MQ=255)

                                                                                                                 tGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCtgtg                                     <  2:3508679/150‑1 (MQ=255)

                                                                                                                  ggATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTga                                    <  2:8631204/150‑1 (MQ=255)

                                                                                                                  ggATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTga                                    <  2:6562335/150‑1 (MQ=255)

                                                                                                                  ggATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTga                                    <  2:8631336/150‑1 (MQ=255)

                                                                                                                  ggATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTga                                    <  2:5820382/150‑1 (MQ=255)

                                                                                                                  ggAGGGGGTAGCTATATCGACGG‑GGGGCGG‑CCGG‑GAGGCCGCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTga                                    <  1:7668065/150‑1 (MQ=21)

                                                                                                                      ggTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATc                                <  2:2323020/150‑1 (MQ=255)

                                                                                                                        tttAGATATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGa                              <  1:6495244/148‑1 (MQ=255)

                                                                                                                        tgtAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGa                              <  1:7226462/150‑1 (MQ=255)

                                                                                                                          tAGCTATATCGACGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAgc                            <  1:5956526/150‑1 (MQ=255)

                                                                                                                             cTATATAGAAGG‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGcgcc                         <  1:6772974/150‑1 (MQ=255)

                                                                                                                               atataGACGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGcgccgc                       <  1:1126220/150‑1 (MQ=255)

                                                                                                                                   aGAAGC‑GGGGAGG‑CCGG‑TAGGCCAACCCGC‑GTTTTTTTTGGGGTTTTGTGGTTTCTGTTGTAGGGGGAGGGGGGTGTTTTTAGAGGGTGTTTGCGGTGAGATGTTTACGCCACAACCCAAGCATCGCCAGTTAGATAGAGAGcccccccc                   >  2:1071735/1‑148 (MQ=255)

                                                                                                                                       gC‑GGGGAGG‑CCGG‑TAGGCC‑CCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCg              <  2:6683948/150‑1 (MQ=255)

                                                                                                                                           gggAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGc            >  2:2862799/1‑150 (MQ=255)

                                                                                                                                           gggAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGc            >  2:192179/1‑150 (MQ=255)

                                                                                                                                              aGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTg         <  2:1422411/150‑1 (MQ=255)

                                                                                                                                               gg‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTga        <  2:5520102/150‑1 (MQ=255)

                                                                                                                                               gg‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTga        <  2:5453663/150‑1 (MQ=255)

                                                                                                                                                g‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTgag       >  2:3588276/1‑150 (MQ=255)

                                                                                                                                                g‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTgag       <  2:4574988/150‑1 (MQ=255)

                                                                                                                                                   cGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGAc     <  1:5725998/150‑1 (MQ=255)

                                                                                                                                                    gg‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACa    <  1:688054/150‑1 (MQ=255)

                                                                                                                                                    gg‑TAGGCCTCCCCGC‑ATTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGGTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACa    <  1:3510215/150‑1 (MQ=255)

                                                                                                                                                       tAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACAtt  <  1:1653605/150‑1 (MQ=255)

GTTGATGGTGCAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGC‑GGGGAGG‑CCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATT  >  NC_002947/524029‑524326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`