Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 727,320 A→G 6.6% P5P (CCT→CCC)  PP_0621 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947727,3200AG 6.6% 1998.5 / 80.3 636P5P (CCT→CCC) PP_0621hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (319/271);  new base G (18/24);  total (337/299)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

Only 100 of 639 total aligned reads displayed.

TGCGACTGCTGGCTGACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGCTCTCGCGGCAT  >  NC_002947/727171‑727467
                                                                                                                                                     |                                                                                                                                                   
 gCGACTGCTGGCTGACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAg                                                                                                                                                    <  1:7333016/150‑1 (MQ=255)
   gACTGCTGGCTGACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGc                                                                                                                                                  >  2:6579074/1‑150 (MQ=255)
     ctgctgGCTGACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAG‑CAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTc                                                                                                                                               <  2:8039612/150‑1 (MQ=255)
         tggctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCTTGGGCCTCCCt                                                                                                                                            <  1:2798263/150‑1 (MQ=255)
         tggctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCt                                                                                                                                            >  1:4971094/1‑150 (MQ=255)
          ggctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCTTGGGCCTCCCtt                                                                                                                                           <  1:7934404/150‑1 (MQ=255)
           gctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCTTGGGCCTCCCttt                                                                                                                                          <  2:6081632/150‑1 (MQ=255)
           gctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCttt                                                                                                                                          >  2:5360315/1‑150 (MQ=255)
            ctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCTTGGGCCTCCCtttt                                                                                                                                         <  2:1713936/150‑2 (MQ=255)
            ctgACTTTGTATGTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttc                                                                                                                                         <  2:3039060/150‑1 (MQ=255)
             tgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttca                                                                                                                                        <  2:2783505/150‑1 (MQ=255)
             tgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttca                                                                                                                                        <  1:6966764/150‑1 (MQ=255)
             tgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttca                                                                                                                                        >  1:7611363/1‑150 (MQ=255)
             tgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttca                                                                                                                                        <  2:3174782/150‑1 (MQ=255)
               aCTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCTTGGGCCTCCCtttttta                                                                                                                                      <  1:2676102/150‑5 (MQ=255)
                 tttGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttcattca                                                                                                                                    >  2:4956063/1‑150 (MQ=255)
                 tttGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttcattca                                                                                                                                    <  2:1167886/150‑1 (MQ=255)
                 tttGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttcattca                                                                                                                                    >  1:5492306/1‑150 (MQ=255)
                 tttGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttcattca                                                                                                                                    >  1:5075770/1‑150 (MQ=255)
                 tttGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttcattca                                                                                                                                    <  1:3762666/150‑1 (MQ=255)
                 gttGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATATAAAAGGGAGGCCTTGGGCCTCCCttttttatt                                                                                                                                    <  2:5783581/149‑7 (MQ=255)
                    ccctgatcgcCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCg                                                                                                                                 >  1:1427879/8‑150 (MQ=255)
                      aTTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCt                                                                                                                               <  2:592287/150‑1 (MQ=255)
                        ttGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGa                                                                                                                             <  2:4360924/150‑1 (MQ=255)
                         tGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGaa                                                                                                                            <  1:3540350/150‑1 (MQ=255)
                         tGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGaa                                                                                                                            <  1:1801889/150‑1 (MQ=255)
                         tGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGaa                                                                                                                            <  1:3351136/150‑1 (MQ=255)
                                   ccAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGa                                                                                                                  >  1:3621512/1‑150 (MQ=255)
                                     aGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAAc                                                                                                                <  1:1542077/150‑1 (MQ=255)
                                       aaCGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAg                                                                                                              >  2:3375167/1‑150 (MQ=255)
                                       aaCGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAg                                                                                                              >  1:1959417/1‑150 (MQ=255)
                                       aaCGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAg                                                                                                              <  2:4825817/150‑1 (MQ=255)
                                         cgcgGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGAc                                                                                                            <  1:3673192/150‑1 (MQ=255)
                                             gcggcgATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCaaa                                                                                                        <  2:2181832/150‑1 (MQ=255)
                                              cggcgATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAg                                                                                                       <  2:5674986/150‑1 (MQ=255)
                                               ggcgATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAgg                                                                                                      >  2:7281883/1‑150 (MQ=255)
                                                 cgATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTc                                                                                                    <  1:6091796/150‑1 (MQ=255)
                                                  gATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttcat                                                                                                                                       <  1:2381410/114‑1 (MQ=255)
                                                  gATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCa                                                                                                   <  1:8255562/150‑1 (MQ=255)
                                                      aGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTa                                                                                               >  1:6480270/1‑150 (MQ=255)
                                                      aGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTa                                                                                               >  1:4251130/1‑150 (MQ=255)
                                                       gggCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAg                                                                                              >  2:744072/1‑150 (MQ=255)
                                                         gCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCt                                                                                            >  2:686199/1‑150 (MQ=255)
                                                           gATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGa                                                                                          <  1:442149/150‑1 (MQ=255)
                                                             tCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAAc                                                                                        >  1:6733049/1‑150 (MQ=255)
                                                               cAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAAAAGACCAAATGTCATGTATCAGAACCa                                                                                      >  1:5986805/1‑150 (MQ=255)
                                                                aGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAg                                                                                     >  1:7213517/1‑150 (MQ=255)
                                                                aGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAg                                                                                     >  1:1300939/1‑150 (MQ=255)
                                                                aGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTGTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAg                                                                                     >  1:4488209/1‑150 (MQ=255)
                                                                  aaaaGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGa                                                                                   >  2:2549623/1‑150 (MQ=255)
                                                                   aaaGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGaa                                                                                  >  1:4784231/1‑150 (MQ=255)
                                                                     aGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACg                                                                                <  2:8296965/150‑1 (MQ=255)
                                                                     aGTAGGCGAACCATGGCGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACg                                                                                <  2:2070205/150‑1 (MQ=255)
                                                                            gAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTtc                                                                         >  1:3184064/1‑150 (MQ=255)
                                                                             aaCCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTtct                                                                        >  2:4750017/1‑150 (MQ=255)
                                                                              aCCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTtctc                                                                       <  2:7648388/150‑1 (MQ=255)
                                                                                cATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCgg                                                                     >  1:8091801/1‑150 (MQ=255)
                                                                                cATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCgg                                                                     <  2:2338309/150‑1 (MQ=255)
                                                                                       tCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCttt                                                              <  2:6405358/150‑1 (MQ=255)
                                                                                         cAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCg                                                            >  2:5304916/1‑150 (MQ=255)
                                                                                          aGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCgg                                                           <  1:8793960/150‑1 (MQ=255)
                                                                                            cTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGc                                                         <  2:3926068/150‑1 (MQ=255)
                                                                                            cTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGc                                                         >  2:6665177/1‑150 (MQ=255)
                                                                                               gTCGATTGAAGAAGCCAACACTCTAGGACGCGCCCAAAAAAAAAGGGACGCCCAAGGCCTCCCTTTCATTCACCGCAGAAGGTCAGTCGAACAGACCAAACGTCATGTAGCTGAACCAGGAACGGTCTATCTCGGCTTCTATCGGGCCta                                                      >  2:845238/1‑149 (MQ=255)
                                                                                                tCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTc                                                     >  2:137237/1‑150 (MQ=255)
                                                                                                 cGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTcc                                                    <  1:1103725/150‑1 (MQ=255)
                                                                                                  gATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGAc                                                                                                            >  1:6585503/1‑93 (MQ=255)
                                                                                                  gATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGAc                                                                                                            >  1:2137964/1‑93 (MQ=255)
                                                                                                   cttGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAATGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCGTCTTTCGGGCCTTCCgg                                                  >  1:85380/2‑150 (MQ=255)
                                                                                                   aTTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAGAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCgg                                                  <  1:3918563/150‑1 (MQ=255)
                                                                                                   aTTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCgg                                                  >  2:6281002/1‑150 (MQ=255)
                                                                                                       aagaagCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACg                                              >  2:7003100/1‑150 (MQ=255)
                                                                                                        agaagCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGa                                             >  2:1884893/1‑150 (MQ=255)
                                                                                                         gaagCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGAt                                            >  2:4498605/1‑150 (MQ=255)
                                                                                                            gCCAACAGTCTAGTACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTGACGGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGTACGGTCTTTCTCGGCTTGTTTCGGGCCTTCCGGCACGATTgg                                         <  2:3516802/150‑1 (MQ=255)
                                                                                                               aaCAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTc                                      >  2:4300973/1‑150 (MQ=255)
                                                                                                               aaCAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTc                                      >  2:4024813/1‑150 (MQ=255)
                                                                                                                aCAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTc                                                             <  1:2264132/126‑1 (MQ=255)
                                                                                                                      tAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTtc                               >  2:7904900/1‑150 (MQ=255)
                                                                                                                           cgcgGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTc                          <  2:1554982/150‑1 (MQ=255)
                                                                                                                            gcgGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGc                                                                    >  2:503463/1‑107 (MQ=255)
                                                                                                                              ggCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCttt                       >  2:3295770/1‑150 (MQ=255)
                                                                                                                               gCCAATACAAAAGGGAGGCCCACGGCCTCCCTTTCAGTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTa                      <  2:2469572/150‑1 (MQ=255)
                                                                                                                               gCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTa                      <  1:5741545/150‑1 (MQ=255)
                                                                                                                               gCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTa                      <  1:1031140/150‑1 (MQ=255)
                                                                                                                               gCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTa                      <  2:3621512/150‑1 (MQ=255)
                                                                                                                                 caatcccaaaGGGAGGCCCAAGGCCTCCCTTTCCTTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAgg                    <  2:7152172/143‑1 (MQ=255)
                                                                                                                                 cAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGACGGTCAGTCGAACAGACCAGAGGGCCTGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAgg                    >  1:254940/1‑150 (MQ=255)
                                                                                                                                 cAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAgg                    <  2:2869283/150‑1 (MQ=255)
                                                                                                                                  tgaataaaaGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCCAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTGTAGgc                   >  1:3045354/6‑150 (MQ=255)
                                                                                                                                   gaatgaaaGGGAGGCCTTGGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGgca                  >  1:5165159/6‑150 (MQ=255)
                                                                                                                                    tAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGgcag                 >  1:7791214/1‑150 (MQ=255)
                                                                                                                                    tAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGgcag                 <  1:5360005/150‑1 (MQ=255)
                                                                                                                                      tgaaaGGGAGGCCTTGGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGgcagca               >  1:1672297/3‑150 (MQ=255)
                                                                                                                                      tgaaaGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGgcagca               >  1:7085395/3‑150 (MQ=255)
                                                                                                                                      aaaaaGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGgcagca               >  1:838519/1‑150 (MQ=255)
                                                                                                                                           gggAGGCCTTGGGCCTCCCGTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTGCTCGTCTGTAGGCAGCAGctct          >  1:99708/1‑150 (MQ=255)
                                                                                                                                            ggAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGCtctc         >  2:5132477/1‑150 (MQ=255)
                                                                                                                                                  ccAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGCTCTCGCGGCa   >  1:6327958/1‑150 (MQ=255)
                                                                                                                                                   cAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGCTCTCGCGGCAt  >  1:4599899/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TGCGACTGCTGGCTGACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGCTCTCGCGGCAT  >  NC_002947/727171‑727467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑