BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	733,648	A→T	5.9%	intergenic (+19/‑28)	PP_t09 → / → PP_t10	tRNA‑Lys/tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	733,648	0	A	T	5.9%	1017.5 / 30.5	360	intergenic (+19/‑28)	PP_t09/PP_t10	tRNA‑Lys/tRNA‑Pro
Reads supporting (aligned to +/- strand): ref base A (190/124); new base T (12/10); total (203/157)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 377 total aligned reads displayed.

CTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACC  >  NC_002947/733499‑733797

  gCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCaaa                                                                                                                                                      <  1:4887775/150‑1 (MQ=255)

    tttGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAaga                                                                                                                                                    >  1:5177859/1‑150 (MQ=255)

    tttGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAaga                                                                                                                                                    <  2:3251579/150‑1 (MQ=255)

    tttGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTAAACAAATTGTTCGTATGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAaga                                                                                                                                                    >  2:5883069/1‑150 (MQ=255)

       gggAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGAt                                                                                                                                                 <  2:1489655/150‑1 (MQ=255)

       gggAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTGTTAACCAATGGGTCGTAGGTTCGAATCGTCCTCGACCCTCCTGGTAGAAATGGGCAGCTCagggggtg                                                                                                                                                 >  2:7503318/1‑143 (MQ=14)

        ggAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATg                                                                                                                                                <  1:5040160/150‑1 (MQ=255)

          aGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCTTTGAGATGcc                                                                                                                                              <  2:7994501/150‑1 (MQ=255)

           gCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCCAAGAGATGccc                                                                                                                                             <  1:7168897/150‑1 (MQ=255)

           gCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGccc                                                                                                                                             <  2:3589221/150‑1 (MQ=255)

           gCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGccc                                                                                                                                             <  2:11892/150‑1 (MQ=255)

           gCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGccc                                                                                                                                             <  2:8491588/150‑1 (MQ=255)

               gATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCtttt                                                                                                                                         <  2:2261487/150‑1 (MQ=255)

               gATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTACAAAAGGGCATCTCAAAGAGAACCCCtttt                                                                                                                                         >  1:1123172/1‑150 (MQ=255)

                 tGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCtttt‑tt                                                                                                                                      <  2:1691659/150‑1 (MQ=255)

                  gTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCGTTGAGATGCCCttttcta                                                                                                                                      <  1:2259651/150‑4 (MQ=255)

                   tCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCtttt‑tttt                                                                                                                                    <  2:3024407/150‑1 (MQ=255)

                    cAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGTTGCCCtttt‑tttgg                                                                                                                                   >  1:3163104/1‑148 (MQ=255)

                    cAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTg                                                                                                                                   <  2:3410100/150‑1 (MQ=255)

                     aGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCACAGAGATGCCCTTTT‑TTTTGa                                                                                                                                  <  1:8354315/150‑1 (MQ=255)

                     aGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGa                                                                                                                                  <  2:3794605/150‑1 (MQ=255)

                             gAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCCGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTAAACGACCCACCGTGTAGAAAAGGGCATCTCAAAGAGTTGGCGTTTTGTTTTTAAacagtg                                                                                                                           >  2:4795708/1‑147 (MQ=17)

                             gAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCATACACGACCCACCATGAACAAAAGCCAAAAGCAAAGAGATGCCATTTT‑TATTTAAACAATCg                                                                                                                          >  2:123482/1‑150 (MQ=14)

                              aaTCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCgg                                                                                                                         >  2:6586010/1‑150 (MQ=255)

                                tCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCgggg                                                                                                                       <  2:390935/150‑1 (MQ=255)

                                   ccTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCAAAAT‑TATTTAAACACTTGGGgtga                                                                                                                    >  1:1709438/1‑149 (MQ=18)

                                        ccccGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAgcgc                                                                                                               >  1:5891137/1‑150 (MQ=255)

                                          ccGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAAATGGTCGTAGGTTCGAAACCTAAACGACCCAACAAGTAGAAAAGGGCATATCAAAGTGATTCCCTTTA‑TTTTGAAACACGCGGGGTGTAGCGCAg                                                                                                             >  1:4662738/1‑150 (MQ=21)

                                            gACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTc                                                                                                           >  2:484979/1‑150 (MQ=255)

                                               cATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTCGGTTCGCATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCAGTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCgg                                                                                                        <  1:2314187/150‑1 (MQ=255)

                                                 ttttCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCTCAGTCCggga                                                                                                      >  1:7924587/1‑148 (MQ=255)

                                                   ttCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAgc                                                                                                    >  1:897781/1‑150 (MQ=255)

                                                              tAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTA‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCgtt                                                                                         >  2:5234325/1‑150 (MQ=255)

                                                               aGCTCAGTTGGTAGAGCATTTGTCTTTTAACCAATTGGTCGTATGTTAGAATCCTAAACGACCCACCATGTATAAATGGGAATCTCAAAGTGATGCCCTTTT‑TTTTGAAACTCTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTg                                                                                        <  2:8196338/150‑1 (MQ=25)

                                                                gCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                       <  2:5806615/150‑1 (MQ=255)

                                                                gCTCAGTGGGTAGAGCAGTTGGCTTTTACCCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCCAAGCGCTGCCCTTTT‑TTTTGACACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                       <  2:8788178/150‑1 (MQ=25)

                                                                  tCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGa                                                                                     >  2:3730091/1‑150 (MQ=255)

                                                                  tCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGa                                                                                     <  1:6017635/150‑1 (MQ=255)

                                                                     gtcgggagagCAGTTGTCTTTTAACCAATTGGTCGTAGGTTCGACTCGGTCAGTTACCTGACTGCAGAATAGGGCATCTCCGATATTTGCACTTTT‑TTTTGAAACTCTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCa                                                                                  <  1:4471146/144‑1 (MQ=12)

                                                                        ggcagagCAGTGGGCATGTACCCAATCGGACGTAGGCACGAATCCTACACGCCCCACCCTGTAGACAAGGGCATCTCCAACAGATGCCCTTCT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGa                                                                               <  2:6775007/147‑1 (MQ=25)

                                                                        ggTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCtttt‑ttt                                                                                                                                     >  1:3591617/1‑96 (MQ=255)

                                                                        ggTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCtttt‑ttt                                                                                                                                     >  1:8663236/1‑96 (MQ=255)

                                                                        ggTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCACGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAACCACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGa                                                                               <  2:4932717/150‑1 (MQ=255)

                                                                          tAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATg                                                                             >  2:4406479/1‑150 (MQ=255)

                                                                          tAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAAGAAAAGGGCATATCAAAGAGATGCCCTTTG‑ATTTGAAACACTCGGGGTGTAGCGCACTACGGTAGCGCGCCTGATTTGGGAGCAGGATg                                                                             >  2:6052014/1‑150 (MQ=25)

                                                                             agCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCa                                                                          <  2:2573671/150‑1 (MQ=255)

                                                                                aGTTGGCTTAGAACCAATTGGTCGTAGGTTCGAATCACACACGACCCACCATGTAGAAAAGGGCATCTCAAAGTGATGCACTTTT‑TTTTGAAACTCTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGa                                                                       <  2:5412624/150‑1 (MQ=37)

                                                                                  ttGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGCAGCGCAGTCCGGTATCGCGCCTGCTTTGGGAGCAGGCTGTCAGCAGt                                                                     >  2:5602351/1‑150 (MQ=255)

                                                                                   tGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGtt                                                                    <  1:2759775/150‑1 (MQ=255)

                                                                                       ttttAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGaa                                                                >  2:735184/1‑150 (MQ=255)

                                                                                       ttttAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGaa                                                                >  2:2357564/1‑150 (MQ=255)

                                                                                       ctttAACCACTCGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCGTTTT‑TTTTGACACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGaa                                                                <  2:4553864/149‑1 (MQ=255)

                                                                                             ccAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAGAAGGGCAAAACAAAGAGATGCCCTTTT‑TTTTTTAACACTAGGGGTGTAGCGCACTCCGGTCGCGCGCCAGCTGTGGTAGCATGATGTCACGGGGTAGAATCCCCt                                                          >  1:3940800/1‑150 (MQ=21)

                                                                                             ccAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTACAAAAGGCAATCACAAAGAGATGACCTTAT‑TTGTGAAACACTCGGGGTGTAGAGCAGACCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCt                                                          >  2:295549/1‑150 (MQ=25)

                                                                                                 ttGGTCGTAGGTTCGAATCCTACACGACACACCATGTAGAAAAGGGCAGCTCAAAGAGATGCCCTTTT‑TTTTTACACACTCTGGGTGGTGCGCTGGCCGGGCGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCAcc                                                      >  1:3014606/1‑150 (MQ=25)

                                                                                                      cGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGAcc                                                >  1:7714160/1‑150 (MQ=255)

                                                                                                        tAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCa                                               >  2:5048291/1‑150 (MQ=255)

                                                                                                        tAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCa                                               >  1:1558315/1‑150 (MQ=255)

                                                                                                         aGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGAGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCAt                                              >  1:8544786/1‑150 (MQ=255)

                                                                                                         aGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTC‑AAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCAtt                                             >  1:8154092/1‑150 (MQ=255)

                                                                                                           gTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCAttt                                            >  2:2926078/1‑150 (MQ=255)

                                                                                                            ttCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCAtttt                                           >  1:7819639/1‑150 (MQ=255)

                                                                                                             tCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCttt                                                                                         <  2:8549384/103‑1 (MQ=255)

                                                                                                                  tCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTc                                     >  1:3686122/1‑150 (MQ=255)

                                                                                                                  tCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTc                                     >  1:1607614/1‑150 (MQ=255)

                                                                                                                   ccTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCg                                    >  1:5671601/1‑150 (MQ=255)

                                                                                                                    cTACACGACCGACCATGTAGAAAAGGGCATCTGTAAGAGATGCCCTTTT‑TTTTGAAACTCTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGt                                   <  2:4618382/150‑1 (MQ=255)

                                                                                                                    cTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGt                                   <  1:2160612/150‑1 (MQ=255)

                                                                                                                     tACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGtt                                  >  2:2142112/1‑150 (MQ=255)

                                                                                                                     tACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCACCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGtt                                  >  1:7462716/1‑150 (MQ=255)

                                                                                                                       cacGACCCACCATGTAGAAAAGGGCCTCTCAAAGAGATGCCCTTTT‑TGTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGGCAGGAGTTCGAATCCCCTCACCCCGACCATGTTTGGGTCGTTAg                                >  2:7039340/1‑150 (MQ=255)

                                                                                                                         cGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCt                              >  1:7302358/1‑150 (MQ=255)

                                                                                                                          gACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCgg                                                                                                                         >  1:1412875/1‑58 (MQ=255)

                                                                                                                             ccaccaTGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGt                          >  2:690746/1‑150 (MQ=255)

                                                                                                                              caccaTGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGtt                         >  2:7269356/1‑150 (MQ=255)

                                                                                                                               acgtagTAGAAAAGGGCATAACTCAGATATGCACTTTT‑ATTAGAAAAACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTg                        <  1:1188153/145‑1 (MQ=17)

                                                                                                                               accaTGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTg                        >  2:6000285/1‑150 (MQ=255)

                                                                                                                               accaTGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTGTTGGGTCGTTAGCTCAGTTg                        >  1:6915245/1‑150 (MQ=255)

                                                                                                                                 caTGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATGTTTGGGTCGTTAGCTCAGTTGGt                      >  1:996092/1‑150 (MQ=255)

                                                                                                                                  aTGTAGAAAAGGGCATCTCACACAGATGCCCAAAT‑TTTTGAAACACTCGGGGTGGAGCGGAGGCCGGTTGAGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTa                     <  2:6439325/150‑1 (MQ=18)

                                                                                                                                  aTGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTa                     >  1:5340796/1‑150 (MQ=255)

                                                                                                                                     aaaaaaaGGGCATCTCTTTGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTagag                  >  1:6389896/4‑150 (MQ=255)

                                                                                                                                      aGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGc                 >  2:2730603/1‑150 (MQ=255)

                                                                                                                                          aaGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGtt             <  1:5735764/150‑1 (MQ=255)

                                                                                                                                          aaGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGtt             >  1:3321100/1‑150 (MQ=255)

                                                                                                                                          aaGGGCATCTCAAAGAGATGCCCTTTT‑GTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGtt             >  1:2871150/1‑150 (MQ=255)

                                                                                                                                            gggCATCTCTTTGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTgg           >  2:4037701/1‑150 (MQ=255)

                                                                                                                                            gggCATCTCTTTGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTgg           >  2:8162063/1‑150 (MQ=255)

                                                                                                                                             ggCATCTCTTTGAGATGCCCTTTT‑TTTTGAAATACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGc          >  1:3074185/1‑150 (MQ=255)

                                                                                                                                             ggCATCTCACTGATAAGCCCATTT‑TTTTCATTCACTGGTGTTCTACCGCGTTCCGTTAGCTCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGc          <  1:4329389/150‑1 (MQ=14)

                                                                                                                                             ggCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTCGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGc          >  1:1344405/1‑150 (MQ=255)

                                                                                                                                                 tctcAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCtttt      <  1:6586010/150‑1 (MQ=255)

                                                                                                                                                   tcAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTCGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTaa    >  2:762713/1‑150 (MQ=255)

                                                                                                                                                   tcAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTCGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTaa    >  1:6262022/1‑150 (MQ=255)

                                                                                                                                                   tcAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTaa    >  2:724309/1‑150 (MQ=255)

                                                                                                                                                   tcAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTaa    >  1:1853233/1‑150 (MQ=255)

                                                                                                                                                   tcAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTaa    >  2:3604997/1‑150 (MQ=255)

                                                                                                                                                   tcAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTaa    >  1:4108524/1‑150 (MQ=255)

                                                                                                                                                     aaaGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAAcc  >  2:3132984/1‑150 (MQ=255)

                                                                                                                                                     aaaGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAAcc  >  2:5678732/1‑150 (MQ=255)

CTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTT‑TTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACC  >  NC_002947/733499‑733797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`