BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	88,653	T→A	5.5%	Q222L (CAG→CTG)	betC ←	choline‑sulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	88,653	0	T	A	5.5%	1232.3 / 37.3	362	Q222L (CAG→CTG)	betC	choline‑sulfatase
Reads supporting (aligned to +/- strand): ref base T (141/201); new base A (9/11); total (150/212)
Fisher's exact test for biased strand distribution p-value = 8.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 367 total aligned reads displayed.

TCTGCAGCAATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACATGGTCGTACAGG  >  NC_002947/88504‑88802

tCTGCAGCAATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCt                                                                                                                                                       <  1:1258440/150‑1 (MQ=255)

tCTGCAGCAATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCt                                                                                                                                                       <  1:3177771/150‑1 (MQ=255)

  tGCAGCAATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTgg                                                                                                                                                     <  2:5398677/150‑1 (MQ=255)

   gcagcaATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGc                                                                                                                                                    <  1:1627077/150‑1 (MQ=255)

   gcagcaATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGc                                                                                                                                                    <  2:367666/150‑1 (MQ=255)

   gcagcaATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGc                                                                                                                                                    <  2:2060829/150‑1 (MQ=255)

    cagcaATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGTGCTGTGAATGCGGGTCGAGTTCTGCCTGGCt                                                                                                                                                   <  2:734420/150‑1 (MQ=255)

    cagcaATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCt                                                                                                                                                   <  2:7630902/150‑1 (MQ=255)

     agcaATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTg                                                                                                                                                  <  2:8180683/150‑1 (MQ=255)

     agcaATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTg                                                                                                                                                  <  2:7971524/150‑1 (MQ=255)

     agcaATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTg                                                                                                                                                  >  1:6144345/1‑150 (MQ=255)

       caATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGaa                                                                                                                                                <  1:5880463/150‑1 (MQ=255)

       caATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGaa                                                                                                                                                <  1:3765294/150‑1 (MQ=255)

         aTTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCAGCCAGGCAGAACt                                                                                                                                              <  2:6354144/150‑1 (MQ=255)

          ttGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCAGCCAGGCAGAACTc                                                                                                                                             <  1:4671595/150‑1 (MQ=255)

          atGGCCGATGTAGACATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACACGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCAGCCAGGCAGAACTc                                                                                                                                             <  2:6579368/149‑1 (MQ=255)

            ggCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCgg                                                                                                                                           <  1:7233416/150‑1 (MQ=255)

            ggCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCgg                                                                                                                                           <  1:5157082/150‑1 (MQ=255)

             gCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCAGCCAGGCAGAACTCgac                                                                                                                                          <  1:951017/150‑3 (MQ=255)

              ccGATGTTGTCATCGATATAGCTGCACGCGCCAAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGTGCCGAGTTCAGCCAGGCAGAACTCgacc                                                                                                                                         <  2:4558741/150‑4 (MQ=255)

                gATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACg                                                                                                                                       >  2:4918260/1‑150 (MQ=255)

                 aTGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACgg                                                                                                                                      <  2:2816613/150‑1 (MQ=255)

                       tCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATg                                                                                                                                >  1:6520670/1‑150 (MQ=255)

                         aTCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATggg                                                                                                                              <  1:7086688/150‑1 (MQ=255)

                         aTCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATggg                                                                                                                              >  2:5107285/1‑150 (MQ=255)

                         aTCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATggg                                                                                                                              <  1:4489990/150‑1 (MQ=255)

                          tCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATgggg                                                                                                                             <  2:5299102/150‑1 (MQ=255)

                          tCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATgggg                                                                                                                             <  1:6971893/150‑1 (MQ=255)

                          tCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATgggg                                                                                                                             <  2:69357/150‑1 (MQ=255)

                           cGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGa                                                                                                                            <  2:1451850/150‑1 (MQ=255)

                               ataGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATc                                                                                                                        <  2:1779333/150‑1 (MQ=255)

                               ataGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATc                                                                                                                        <  1:2855796/150‑1 (MQ=255)

                                     gCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGATGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACAcc                                                                                                                  >  1:3896859/1‑150 (MQ=255)

                                        cgcgCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTc                                                                                                               <  1:7809716/150‑1 (MQ=255)

                                        cgcgCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGAGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTc                                                                                                               <  2:4386891/150‑1 (MQ=255)

                                         gcgcCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCg                                                                                                              <  1:549374/150‑1 (MQ=255)

                                           gcCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGTTATCCACACCTTCGTa                                                                                                            <  2:1264953/150‑1 (MQ=255)

                                           gcCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTa                                                                                                            >  2:6248975/1‑150 (MQ=255)

                                           gcCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTa                                                                                                            <  1:4898486/150‑1 (MQ=255)

                                           gcCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTa                                                                                                            >  1:6737585/1‑150 (MQ=255)

                                            ccGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAg                                                                                                           <  2:4124140/150‑1 (MQ=255)

                                             cGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGc                                                                                                          >  1:6326116/1‑150 (MQ=255)

                                               aaGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCgg                                                                                                        >  2:1669470/1‑150 (MQ=255)

                                                aGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGt                                                                                                       <  2:2499282/150‑1 (MQ=255)

                                                  tAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCTTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTcc                                                                                                     <  2:5650772/150‑1 (MQ=255)

                                                  tAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTcc                                                                                                     <  2:580238/150‑1 (MQ=255)

                                                    ggCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCa                                                                                                   <  2:2370470/150‑1 (MQ=255)

                                                    ggCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCa                                                                                                   <  2:2207336/150‑1 (MQ=255)

                                                    ggCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCa                                                                                                   <  1:8160791/150‑1 (MQ=255)

                                                       gcggcgGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTa                                                                                                <  2:3160851/150‑1 (MQ=255)

                                                             ggCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCTAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCGTGGCTGTACTCGGCACTGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTATCGttt                                                                                          <  2:1023727/150‑1 (MQ=255)

                                                                      aaCCTTATCCACAGGCAGTGGCTTGTTCCAAACGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGgt                                                                                 <  1:8258121/150‑1 (MQ=255)

                                                                           tATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAgg                                                                            <  2:2742896/150‑1 (MQ=255)

                                                                           tATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAgg                                                                            <  1:4169570/150‑1 (MQ=255)

                                                                            aTCCACAGGCAGTGGCTTGGTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAggg                                                                           <  2:6819559/150‑1 (MQ=255)

                                                                             tCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGAAGCGTTTGGCAATGGTGTAgggg                                                                          >  2:6521117/1‑150 (MQ=255)

                                                                                     cAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCgg                                                                  <  1:8382310/150‑1 (MQ=255)

                                                                                        tGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCgggtg                                                               >  1:8605651/1‑150 (MQ=255)

                                                                                        tGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCgggtg                                                               <  1:5352562/150‑1 (MQ=255)

                                                                                        tGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCgggtg                                                               <  1:4377355/150‑1 (MQ=255)

                                                                                         ggCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCgggtgg                                                              <  1:1968205/150‑1 (MQ=255)

                                                                                         ggCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCgggtgg                                                              <  2:6047587/150‑1 (MQ=255)

                                                                                            ttGTTCCAATGGTGGTAGTCCTTCAGCAGGCGGTGTGAATGCGGGTCGTGTTCTGGGTGGCTGAACTCGGCAGGGGGGATGGGGATATCGACAGGTTCGTAGCGGTCGCTGTAGCGTTTGGCTATGGTGTAGGGGTCATGCGGGTGGGTc                                                           >  2:7415188/1‑150 (MQ=255)

                                                                                            ttGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCAGCCAGGCAGAACTc                                                                                                                                             <  2:7580492/68‑1 (MQ=38)

                                                                                            ttGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGCCGAGTTCAGCCAGGca                                                                                                                                                   <  1:4253039/62‑2 (MQ=255)

                                                                                               ttCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATc                                                        <  1:3535349/150‑1 (MQ=255)

                                                                                                  cAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGaa                                                     >  1:2631014/1‑150 (MQ=255)

                                                                                                   aaaGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGaaa                                                    >  1:2779628/1‑150 (MQ=255)

                                                                                                    aaGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAAc                                                   >  2:156836/1‑150 (MQ=255)

                                                                                                       gTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGt                                                >  2:5244223/1‑150 (MQ=255)

                                                                                                        tCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTc                                               <  2:3881481/150‑1 (MQ=255)

                                                                                                          gTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAg                                             >  2:3945705/1‑150 (MQ=255)

                                                                                                          gTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAg                                             <  2:8444546/150‑1 (MQ=255)

                                                                                                           tAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAgg                                            >  1:1362226/1‑150 (MQ=255)

                                                                                                           tAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAgg                                            >  1:218250/1‑150 (MQ=255)

                                                                                                             gACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCa                                          >  1:7633980/1‑150 (MQ=255)

                                                                                                              aCCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCaa                                         >  2:8753426/1‑150 (MQ=255)

                                                                                                               ccTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCaaa                                        >  1:7569889/1‑150 (MQ=255)

                                                                                                                cTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCaaaa                                       >  1:1553054/1‑150 (MQ=255)

                                                                                                                    agcagGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATggc                                   <  2:2642343/150‑1 (MQ=255)

                                                                                                                    agcagGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATggc                                   <  2:1803932/150‑1 (MQ=255)

                                                                                                                     gcagGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATggcc                                  <  2:780950/150‑1 (MQ=255)

                                                                                                                     gcagGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATggcc                                  <  1:7381220/150‑1 (MQ=255)

                                                                                                                      cagGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTTGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGTGTCATCGAAACGGTCAGGCAATATgtccg                                 >  1:594383/1‑150 (MQ=255)

                                                                                                                       agGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATggccgg                                <  1:7997492/150‑1 (MQ=255)

                                                                                                                        ggCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATggccggc                               >  1:4694729/1‑150 (MQ=255)

                                                                                                                         gcgcTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGGAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATggccggcc                              >  1:238596/1‑150 (MQ=255)

                                                                                                                            cTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATc                           <  1:1651840/150‑1 (MQ=255)

                                                                                                                             tgtgAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCg                          >  1:3261915/1‑150 (MQ=255)

                                                                                                                                gAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGttt                       >  2:8041823/1‑150 (MQ=255)

                                                                                                                                      gtgccGAGTTCAGCCAGGCAGAACTCGACACGGGGCATGGGGATATACACAACTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAGCGGTCAGGCAAAATGGCCGGCCATCGATTTCgcgc                 >  1:1887376/5‑150 (MQ=255)

                                                                                                                                       ggTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGca                >  1:2135876/1‑150 (MQ=255)

                                                                                                                                          cGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACAt             >  1:4958084/1‑150 (MQ=255)

                                                                                                                                          cGAGTTCAGCCAGGCAGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACAt             >  1:4125159/1‑150 (MQ=255)

                                                                                                                                          cGAGTTCAGCCAGGCAGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACAt             >  2:8146877/1‑150 (MQ=255)

                                                                                                                                          cGAGTTCAGCCAGGCAGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAAGGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACAt             >  2:4992124/1‑150 (MQ=255)

                                                                                                                                              ttCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACATGGTc         <  2:2564335/150‑1 (MQ=255)

                                                                                                                                                  gCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACATGGTCGTAc     <  2:7097286/150‑1 (MQ=255)

                                                                                                                                                  gCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACATGGTCGTAc     <  2:4237075/150‑1 (MQ=255)

                                                                                                                                                   ccTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACATGGTCGTACa    <  2:611964/150‑1 (MQ=255)

TCTGCAGCAATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACATGGTCGTACAGG  >  NC_002947/88504‑88802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`