BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	88,657	T→A	5.1%	S221C (AGC→TGC)	betC ←	choline‑sulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	88,657	0	T	A	5.1%	1207.4 / 32.7	352	S221C (AGC→TGC)	betC	choline‑sulfatase
Reads supporting (aligned to +/- strand): ref base T (142/192); new base A (8/10); total (150/202)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 361 total aligned reads displayed.

CAGCAATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACATGGTCGTACAGGTACT  >  NC_002947/88508‑88806

cagcaATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGTGCTGTGAATGCGGGTCGAGTTCTGCCTGGCt                                                                                                                                                       <  2:734420/150‑1 (MQ=255)

cagcaATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCt                                                                                                                                                       <  2:7630902/150‑1 (MQ=255)

cagcaATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCt                                                                                                                                                       <  2:6291684/150‑1 (MQ=255)

 agcaATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTg                                                                                                                                                      <  2:7971524/150‑1 (MQ=255)

 agcaATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTg                                                                                                                                                      >  1:6144345/1‑150 (MQ=255)

  gcaATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGa                                                                                                                                                     >  1:5171110/1‑150 (MQ=255)

   caATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGaa                                                                                                                                                    <  1:5880463/150‑1 (MQ=255)

   caATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGaa                                                                                                                                                    <  2:1469188/150‑1 (MQ=255)

   caATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGaa                                                                                                                                                    <  1:3765294/150‑1 (MQ=255)

      ttGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTc                                                                                                                                                 <  1:1265378/150‑1 (MQ=255)

        ggCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCgg                                                                                                                                               <  1:7233416/150‑1 (MQ=255)

             aTGTTGACATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACgg                                                                                                                                          <  2:7134329/150‑1 (MQ=255)

                ttgtCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGc                                                                                                                                       <  2:8286185/150‑1 (MQ=255)

                   tCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATg                                                                                                                                    >  1:6520670/1‑150 (MQ=255)

                   tCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATg                                                                                                                                    >  2:4865060/1‑150 (MQ=255)

                     aTCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATggg                                                                                                                                  >  1:1634693/1‑150 (MQ=255)

                     aTCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATggg                                                                                                                                  <  1:7086688/150‑1 (MQ=255)

                     aTCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATggg                                                                                                                                  >  1:4617211/1‑150 (MQ=255)

                      tCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATgggg                                                                                                                                 <  1:1562109/150‑1 (MQ=255)

                      tCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATgggg                                                                                                                                 <  1:36474/150‑1 (MQ=255)

                       cGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCGGTGGATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGa                                                                                                                                <  2:6550157/150‑1 (MQ=255)

                        gATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGat                                                                                                                               <  2:4259765/150‑1 (MQ=255)

                           ataGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATc                                                                                                                            <  2:1711214/150‑1 (MQ=255)

                           ataGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATc                                                                                                                            <  1:5417665/150‑1 (MQ=255)

                           ataGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATc                                                                                                                            >  1:4338481/1‑150 (MQ=255)

                           ataGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATc                                                                                                                            <  2:1779333/150‑1 (MQ=255)

                            taGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATcc                                                                                                                           <  1:2730754/150‑1 (MQ=255)

                              gCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCcac                                                                                                                         <  2:5059537/150‑1 (MQ=255)

                                 gCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGATGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACAcc                                                                                                                      >  1:3896859/1‑150 (MQ=255)

                                   aCGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCtt                                                                                                                    >  2:3696580/1‑150 (MQ=255)

                                    cgcgCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTc                                                                                                                   <  1:7809716/150‑1 (MQ=255)

                                     gcgcCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCg                                                                                                                  <  1:549374/150‑1 (MQ=255)

                                     gcgcCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCg                                                                                                                  >  1:2992606/1‑150 (MQ=255)

                                       gcCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTa                                                                                                                <  1:172309/150‑1 (MQ=255)

                                       gcCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTa                                                                                                                >  1:6737585/1‑150 (MQ=255)

                                        ccGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAg                                                                                                               <  2:4124140/150‑1 (MQ=255)

                                        ccGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAg                                                                                                               >  1:3321866/1‑150 (MQ=255)

                                         cGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGc                                                                                                              >  1:6326116/1‑150 (MQ=255)

                                            aGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCg                                                                                                             <  2:2112833/148‑1 (MQ=255)

                                            aGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCg                                                                                                             >  1:2112833/1‑148 (MQ=255)

                                            aGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGt                                                                                                           <  2:2499282/150‑1 (MQ=255)

                                              tAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCATGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTcc                                                                                                         <  2:5650728/150‑1 (MQ=255)

                                                ggCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCa                                                                                                       <  1:7999648/150‑1 (MQ=255)

                                                ggCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCa                                                                                                       <  1:8160791/150‑1 (MQ=255)

                                                ggCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCa                                                                                                       <  2:67088/150‑1 (MQ=255)

                                                   gcggcgGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTa                                                                                                    <  2:3160851/150‑1 (MQ=255)

                                                          gCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTg                                                                                             >  2:532983/1‑150 (MQ=255)

                                                             tCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCa                                                                                          >  1:2371359/1‑150 (MQ=255)

                                                               gcgAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAAt                                                                                        >  2:6418866/1‑150 (MQ=255)

                                                               gcgAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAAt                                                                                        >  1:95291/1‑150 (MQ=255)

                                                               gcgAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCGGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTCGCGGTCCCAGTAGCGTTTGGCaag                                                                                        >  2:1072528/1‑149 (MQ=255)

                                                                  aaCCTTATCCACAGGCAGTGGCTTGTTCCAAACGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGgt                                                                                     <  1:8258121/150‑1 (MQ=255)

                                                                      ttATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAg                                                                                 <  2:52768/150‑1 (MQ=255)

                                                                       tATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAgg                                                                                <  2:2742896/150‑1 (MQ=255)

                                                                           cacaGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTc                                                                            >  1:4686198/1‑150 (MQ=255)

                                                                           cacaGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTc                                                                            >  2:2563439/1‑150 (MQ=255)

                                                                             caGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCAt                                                                          >  2:4979587/1‑150 (MQ=255)

                                                                                 cAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCgg                                                                      <  1:1935062/150‑1 (MQ=255)

                                                                                   gTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGCTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCgggt                                                                    <  2:7411373/150‑1 (MQ=255)

                                                                                    tGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCgggtg                                                                   >  2:2804264/1‑150 (MQ=255)

                                                                                    tGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCgggtg                                                                   >  1:4175344/1‑150 (MQ=255)

                                                                                     ggCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCgggtgg                                                                  <  1:2799915/150‑1 (MQ=255)

                                                                                     ggCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCgggtgg                                                                  <  2:5164073/150‑1 (MQ=255)

                                                                                     ggCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCgggtgg                                                                  <  1:872521/150‑1 (MQ=255)

                                                                                        ttGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTc                                                               >  1:5026809/1‑150 (MQ=255)

                                                                                        ttGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCAGCCAGGCAGAACTc                                                                                                                                                 <  2:7580492/68‑1 (MQ=38)

                                                                                         tGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCa                                                              >  1:7662435/1‑150 (MQ=255)

                                                                                           ttCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATc                                                            <  1:6965326/150‑1 (MQ=255)

                                                                                              cAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGGGTAGGGGTCATGCGGGTGGGTCATCGaa                                                         >  1:2169283/1‑150 (MQ=255)

                                                                                                  ggTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACgg                                                     >  1:7570012/1‑150 (MQ=255)

                                                                                                    tCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTc                                                   <  2:3881481/150‑1 (MQ=255)

                                                                                                    tCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTc                                                   <  1:2417640/150‑1 (MQ=255)

                                                                                                    tCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTc                                                   >  2:2344486/1‑150 (MQ=255)

                                                                                                     cggaGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCa                                                  <  1:3237287/147‑1 (MQ=255)

                                                                                                     cGTAGGCCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCa                                                  <  2:5711641/150‑1 (MQ=255)

                                                                                                     cGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCa                                                  <  2:6326116/150‑1 (MQ=255)

                                                                                                      gTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAg                                                 <  2:8444546/150‑1 (MQ=255)

                                                                                                        aGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGc                                               <  2:3958070/150‑1 (MQ=255)

                                                                                                         gACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCa                                              >  2:7786732/1‑150 (MQ=255)

                                                                                                         gACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCa                                              >  2:180935/1‑150 (MQ=255)

                                                                                                          aCCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCaa                                             >  2:8753426/1‑150 (MQ=255)

                                                                                                                agcagGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATggc                                       <  2:2642343/150‑1 (MQ=255)

                                                                                                                  cagGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTTGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGTGTCATCGAAACGGTCAGGCAATATgtccg                                     >  1:594383/1‑150 (MQ=255)

                                                                                                                  cagGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATggccg                                     <  1:4464954/150‑1 (MQ=255)

                                                                                                                   agGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATggccgg                                    <  2:7039487/150‑1 (MQ=255)

                                                                                                                            gAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGttt                           >  2:8041823/1‑150 (MQ=255)

                                                                                                                              aTGCGGGCCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTc                         <  2:4528032/150‑1 (MQ=255)

                                                                                                                                   ggTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTc                         >  2:1590/1‑145 (MQ=255)

                                                                                                                                      cGAGTTCAGCCAGGCAGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACAt                 >  2:8146877/1‑150 (MQ=255)

                                                                                                                                      cGAGTTCAGCCAGGCAGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACAt                 >  1:4125159/1‑150 (MQ=255)

                                                                                                                                         gTTCTGCCTGGCTGAACTCGGCACGGGGCCTGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTCGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACATGGt              >  2:6849081/1‑150 (MQ=255)

                                                                                                                                          ttCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACATGGTc             >  1:1471859/1‑150 (MQ=255)

                                                                                                                                             tGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACATGGTCGTa          >  2:5974039/1‑150 (MQ=255)

                                                                                                                                              gCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACATGGTCGTAc         <  1:4918260/150‑1 (MQ=255)

                                                                                                                                               ccTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACATGGTCGTACa        <  2:611964/150‑1 (MQ=255)

                                                                                                                                               ccTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACATGGTCGTACa        <  1:8489403/150‑1 (MQ=255)

                                                                                                                                                cTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACATGGTCGTACAg       <  2:1357969/150‑1 (MQ=255)

                                                                                                                                                 tGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACATGGTCGTACAgg      >  1:7368580/1‑150 (MQ=255)

                                                                                                                                                   gCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACATGGTCGTACAGGTa    <  1:4865060/150‑1 (MQ=255)

                                                                                                                                                    cTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACATGGTCGTACAGGTAc   >  2:6104942/1‑150 (MQ=255)

CAGCAATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACATGGTCGTACAGGTACT  >  NC_002947/88508‑88806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`