Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 870,233 G→T 5.9% R65S (CGC→AGC)  PP_5456 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947870,2330GT 5.9% 2363.2 / 90.2 700R65S (CGC→AGC) PP_5456hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (366/292);  new base T (30/12);  total (396/304)
Fisher's exact test for biased strand distribution p-value = 5.36e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 726 total aligned reads displayed.

ATCGGCGAGTGCGGGGCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCGGGCATAAGCGT  >  NC_002947/870084‑870382
                                                                                                                                                     |                                                                                                                                                     
 tCGGCGAGTGCGGGGCCCGATCAGGAGACGTCCTTATACTCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACAGCGGTTTGCGCGa                                                                                                                                                      <  2:1287046/150‑1 (MQ=255)
   ggCGAGTGCGGGGCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAgc                                                                                                                                                    <  1:8231267/150‑1 (MQ=255)
        gTGCGGGGCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCTCGCGCaaa                                                                                                                                               <  2:6664868/150‑1 (MQ=255)
        gTGCGGGGCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCTCGCGCaaa                                                                                                                                               <  1:4771385/150‑1 (MQ=255)
            ggggCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCg                                                                                                                                           <  1:3982186/150‑1 (MQ=255)
             gggCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCgg                                                                                                                                          <  1:1113630/150‑1 (MQ=255)
             gggCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCgg                                                                                                                                          <  2:5418309/150‑1 (MQ=255)
             gggCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCgg                                                                                                                                          <  2:1801858/150‑1 (MQ=255)
              ggCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCggg                                                                                                                                         >  1:3400881/1‑150 (MQ=255)
                cccGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCt                                                                                                                                       >  1:8054915/1‑150 (MQ=255)
                 ccGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTGTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCACACCCGGGCtt                                                                                                                                      >  2:7646430/1‑150 (MQ=255)
                  cGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCgggacat                                                                                                                                     >  1:5157725/1‑146 (MQ=255)
                  cGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTc                                                                                                                                     >  1:6577450/1‑150 (MQ=255)
                     tCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTcggc                                                                                                                                  <  1:5450409/150‑1 (MQ=255)
                        ggAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCggg                                                                                                                               >  2:5587111/1‑150 (MQ=255)
                        ggAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCggg                                                                                                                               >  2:5181190/1‑150 (MQ=255)
                        ggAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCggg                                                                                                                               >  1:3520687/1‑150 (MQ=255)
                         gagaCGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGc                                                                                                                              >  2:1665802/1‑150 (MQ=255)
                            aCGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGc                                                                                                                           <  2:4285667/150‑1 (MQ=255)
                              gTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTc                                                                                                                         >  2:1522101/1‑150 (MQ=255)
                                 cTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTcgcg                                                                                                                      >  1:787681/1‑150 (MQ=255)
                                  ttATAATCGGGGGGAGATTATAGCGCTGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCgcgc                                                                                                                     <  1:3139952/150‑1 (MQ=255)
                                  ttATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCgcgc                                                                                                                     >  2:4430353/1‑150 (MQ=255)
                                      aaTCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCcca                                                                                                                 >  2:3837256/1‑150 (MQ=255)
                                        tCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCccagc                                                                                                               >  2:498113/1‑150 (MQ=255)
                                        tCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCccagc                                                                                                               >  1:7313580/1‑150 (MQ=255)
                                           gggggAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCccagccag                                                                                                            >  2:6799807/1‑150 (MQ=255)
                                             gggAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAgccaggc                                                                                                          <  1:6269237/150‑1 (MQ=255)
                                               gagaTTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAgccaggcca                                                                                                        >  1:253375/1‑150 (MQ=255)
                                                 gaTTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGc                                                                                                      <  1:1594511/150‑1 (MQ=255)
                                                  aTTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCt                                                                                                     >  2:6083465/1‑150 (MQ=255)
                                                     ataGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGaa                                                                                                  >  1:7066271/1‑150 (MQ=255)
                                                      taGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAAt                                                                                                 >  1:1210463/1‑150 (MQ=255)
                                                          gATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCg                                                                                             <  2:7743468/150‑1 (MQ=255)
                                                           aTGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGc                                                                                            <  2:3997909/150‑1 (MQ=255)
                                                               cgcgGGCAGAAAGGTCGCCATTCGTGGTTTGTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCACCGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGAt                                                                                        <  2:3445879/150‑1 (MQ=255)
                                                               cgccgccAGAAATCTCGCCATTCGTGTTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGACGCGCTGGTGCCAACGGCGGTTTGCGCGACCCCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGAt                                                                                        <  1:237724/144‑1 (MQ=255)
                                                                      aGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCATCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCAcc                                                                                 >  2:4661934/1‑150 (MQ=255)
                                                                      aGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCAcc                                                                                 >  2:2489274/1‑150 (MQ=255)
                                                                      aGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCAcc                                                                                 >  2:223039/1‑150 (MQ=255)
                                                                      aGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCAcc                                                                                 >  2:1012343/1‑150 (MQ=255)
                                                                      aGAAATGTCGCCATTCGTGGTTTTTATTGCAGAGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTAGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTACGCTGATCTGCAcc                                                                                 >  2:3207748/1‑150 (MQ=255)
                                                                        aaaTGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCa                                                                               >  1:1018690/1‑150 (MQ=255)
                                                                        aaaTGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCa                                                                               >  2:8550956/1‑150 (MQ=255)
                                                                             tCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGa                                                                          >  1:5812830/1‑150 (MQ=255)
                                                                                ccATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGc                                                                       <  1:3761702/150‑1 (MQ=255)
                                                                                ccATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGc                                                                       >  2:6970311/1‑150 (MQ=255)
                                                                                  aTTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGccc                                                                     <  1:5960487/150‑1 (MQ=255)
                                                                                   ttCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGcccc                                                                    >  2:7909045/1‑150 (MQ=255)
                                                                                     cGTGTTATTTAGGGCCGCGTGTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGCGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCgt                                                                  <  1:7411377/150‑1 (MQ=255)
                                                                                     cGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCgt                                                                  <  2:8247278/150‑1 (MQ=255)
                                                                                     cGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCgt                                                                  <  2:2887515/150‑1 (MQ=255)
                                                                                        ggTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCgtggt                                                               <  2:2612337/150‑1 (MQ=255)
                                                                                         gTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCgtggtg                                                              >  1:8748701/1‑150 (MQ=255)
                                                                                         gTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCgtggtg                                                              >  1:4629724/1‑150 (MQ=255)
                                                                                         gTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCgtggtg                                                              >  2:64837/1‑150 (MQ=255)
                                                                                          tttttATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGc                                                             >  1:2699689/1‑150 (MQ=255)
                                                                                           ttttATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGcc                                                            >  1:1865198/1‑150 (MQ=255)
                                                                                            tttATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCt                                                           >  2:2739312/1‑150 (MQ=255)
                                                                                             ttATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTg                                                          >  1:3610785/1‑150 (MQ=255)
                                                                                                ttGCAGCGTTTGTCAGGCAGTGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCt                                                       >  1:4943542/1‑150 (MQ=255)
                                                                                                ttGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCt                                                       <  2:2051739/150‑1 (MQ=255)
                                                                                                ttGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCt                                                       >  1:2020696/1‑150 (MQ=255)
                                                                                                     gCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCAtt                                                  >  2:6238344/1‑150 (MQ=255)
                                                                                                      cGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTg                                                 >  1:5318818/1‑150 (MQ=255)
                                                                                                        tttGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCt                                               >  2:1856755/1‑150 (MQ=255)
                                                                                                         ttGTCAGGCAGCGTGGAGGCGCTGGTGCCACCGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCtt                                              <  1:6461780/150‑1 (MQ=255)
                                                                                                            tCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGaa                                           >  2:1371902/1‑150 (MQ=255)
                                                                                                            tCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGaa                                           <  2:418817/150‑1 (MQ=255)
                                                                                                            tCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGaa                                           >  1:4837008/1‑150 (MQ=255)
                                                                                                            tCAGGCAGCGAGGAGGCGCGGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGaa                                           <  1:6066870/150‑1 (MQ=255)
                                                                                                              aGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAgg                                         >  2:7386725/1‑150 (MQ=255)
                                                                                                                 cAGCGTGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCa                                      <  2:8059606/150‑1 (MQ=255)
                                                                                                                  aGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAg                                     >  2:3085532/1‑150 (MQ=255)
                                                                                                                  aGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAg                                     >  1:2291397/1‑150 (MQ=255)
                                                                                                                    cGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGc                                   >  1:1502725/1‑150 (MQ=255)
                                                                                                                     gaggagGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCa                                  >  1:2855264/1‑150 (MQ=255)
                                                                                                                          ggCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGc                             >  2:3674130/1‑150 (MQ=255)
                                                                                                                           gcgcTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGcc                            >  2:5294515/1‑150 (MQ=255)
                                                                                                                           gcgcTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGcc                            >  2:870520/1‑150 (MQ=255)
                                                                                                                               tGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATcc                        >  2:8797170/1‑150 (MQ=255)
                                                                                                                               tGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATcc                        >  1:8324962/1‑150 (MQ=255)
                                                                                                                                   gCCACCGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCAGTGCTTGAAGGCCAGGCATTTGCCATCCGCGt                    <  2:5881445/150‑1 (MQ=255)
                                                                                                                                   gCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGt                    <  2:7254067/150‑1 (MQ=255)
                                                                                                                                      aaCGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCaa                 >  1:7696598/1‑150 (MQ=255)
                                                                                                                                       aCGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAg                >  2:5706189/1‑150 (MQ=255)
                                                                                                                                          gcggTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCg             <  1:687932/150‑1 (MQ=255)
                                                                                                                                          cgggTTTGCGCTCGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCg             >  1:302037/3‑150 (MQ=255)
                                                                                                                                          cgggTTTGCGCTCGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCg             >  2:5090784/3‑150 (MQ=255)
                                                                                                                                           cggTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCgg            <  2:4522201/150‑1 (MQ=255)
                                                                                                                                             gTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCATCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCGGGc          <  2:7522506/150‑1 (MQ=255)
                                                                                                                                              tttGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCGGGCa         >  1:1407172/1‑150 (MQ=255)
                                                                                                                                                 gcgcGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCGGGCATaa      <  1:5631326/150‑1 (MQ=255)
                                                                                                                                                  cgcgAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCGGGCATAAg     <  1:4161884/150‑1 (MQ=255)
                                                                                                                                                  cgcgAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCGGGCATAAg     <  1:3837256/150‑1 (MQ=255)
                                                                                                                                                   gcgAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCGGGCATAAGc    >  1:7515704/1‑150 (MQ=255)
                                                                                                                                                     gAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGACTTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCAGTTGCCATCCGCGTCAAGGCGGGCATACGCGt  >  2:8410607/1‑150 (MQ=255)
                                                                                                                                                     gAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCGGGCATAAGCGt  >  1:862309/1‑150 (MQ=255)
                                                                                                                                                     gAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCGGGCATAAGCGt  >  1:4364069/1‑150 (MQ=255)
                                                                                                                                                     gAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAAATTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCGGGCATAAGCGt  >  2:2350903/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATCGGCGAGTGCGGGGCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCGGGCATAAGCGT  >  NC_002947/870084‑870382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑