Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 873,428 T→C 5.1% L20P (CTT→CCT)  PP_5457 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947873,4280TC 5.1% 2349.1 / 65.4 721L20P (CTT→CCT) PP_5457hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (326/356);  new base C (23/14);  total (350/371)
Fisher's exact test for biased strand distribution p-value = 9.40e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 730 total aligned reads displayed.

ATTGCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTACAATGATGCC  >  NC_002947/873279‑873576
                                                                                                                                                               |                                                                                                                                                    
 ttGCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑tt                                                                                                                                                     <  1:6299327/150‑1 (MQ=255)
    ccAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGcc                                                                                                                                                  <  2:2778532/150‑1 (MQ=255)
    ccAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGcc                                                                                                                                                  >  1:3793295/1‑150 (MQ=255)
     cAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCa                                                                                                                                                 >  1:7256921/1‑150 (MQ=255)
     cAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCa                                                                                                                                                 <  1:7090119/150‑1 (MQ=255)
      aGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAg                                                                                                                                                >  2:3735248/1‑150 (MQ=255)
         tgtgCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGa                                                                                                                                             <  1:2575192/150‑1 (MQ=255)
         tgtgCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGa                                                                                                                                             >  1:7142554/1‑150 (MQ=255)
          gtgCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAg                                                                                                                                            <  2:6341572/150‑1 (MQ=255)
           tgCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAgg                                                                                                                                           <  2:4151603/150‑1 (MQ=255)
           tgCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAgg                                                                                                                                           <  2:5833654/150‑1 (MQ=255)
           tgCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAAGCATGC‑‑‑‑‑‑‑‑‑‑CTGCCAGcattt                                                                                                                                           <  2:6072509/150‑5 (MQ=255)
             cTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGAATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAgggg                                                                                                                                         >  2:4006318/1‑150 (MQ=255)
              tGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGa                                                                                                                                        >  2:8169546/1‑150 (MQ=255)
               gTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGAt                                                                                                                                       >  2:4684643/1‑150 (MQ=255)
               gTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGAt                                                                                                                                       <  1:2153954/150‑1 (MQ=255)
                tCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATc                                                                                                                                      >  2:1287309/1‑150 (MQ=255)
                tCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATc                                                                                                                                      >  2:226755/1‑150 (MQ=255)
                         ggtgTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTacac                                                                                                                             <  2:6786999/150‑1 (MQ=255)
                         ggtgTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTacac                                                                                                                             <  1:7013331/150‑1 (MQ=255)
                             tgCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTgg                                                                                                                         <  2:7761150/150‑1 (MQ=255)
                             tgCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTgg                                                                                                                         >  1:1927675/1‑150 (MQ=255)
                                  tCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGc                                                                                                                    >  1:2864397/1‑150 (MQ=255)
                                       aGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGa                                                                                                               >  1:3023449/1‑150 (MQ=255)
                                         gTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGAcc                                                                                                             >  1:4947022/1‑150 (MQ=255)
                                            cATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTg                                                                                                          <  2:1464417/150‑1 (MQ=255)
                                             aTGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGt                                                                                                         <  2:2487752/150‑1 (MQ=255)
                                                 tGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGt                                                                                                     >  1:969833/1‑150 (MQ=255)
                                                  gACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTg                                                                                                    <  1:4494441/150‑1 (MQ=255)
                                                    cGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTgcc                                                                                                  <  1:1278515/150‑1 (MQ=255)
                                                     gAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTgccg                                                                                                 >  1:2294616/1‑150 (MQ=255)
                                                     gAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAAGCATGCCTGCCAGCATTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCtt                                                                                                           >  1:4178313/1‑150 (MQ=255)
                                                        tGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGccgccg                                                                                              >  2:8429415/1‑150 (MQ=255)
                                                         ggATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCgg                                                                                             <  2:3491524/150‑1 (MQ=255)
                                                          gATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGc                                                                                            <  2:8644587/150‑1 (MQ=255)
                                                          gATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGc                                                                                            >  2:1201979/1‑150 (MQ=255)
                                                            tGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGa                                                                                          <  1:2692378/150‑1 (MQ=255)
                                                             gCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAg                                                                                         >  1:8456311/1‑150 (MQ=255)
                                                               ttGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCt                                                                                       >  2:3900018/1‑150 (MQ=255)
                                                               ttGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCt                                                                                       <  1:7854640/150‑1 (MQ=255)
                                                                  aaGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGcc                                                                                    >  2:7087601/1‑150 (MQ=255)
                                                                     tCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCtt                                                                                 <  1:5662129/150‑1 (MQ=255)
                                                                       cgccgcGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCg                                                                                                       <  1:8338620/126‑1 (MQ=255)
                                                                         ccgcGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGATGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAg                                                                             <  2:1837851/150‑1 (MQ=255)
                                                                         ccgcGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAg                                                                             <  2:5301074/150‑1 (MQ=255)
                                                                         ccgcGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAg                                                                             <  2:5110275/150‑1 (MQ=255)
                                                                          cgcgAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAgg                                                                            <  2:5885141/150‑1 (MQ=255)
                                                                          cgcgAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAgg                                                                            <  2:7423823/150‑1 (MQ=255)
                                                                            cgAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGc                                                                          <  1:5992916/150‑1 (MQ=255)
                                                                                   gacACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTgg                                                                   >  1:4650268/1‑150 (MQ=255)
                                                                                     cacCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTgggg                                                                 <  1:6698158/150‑1 (MQ=255)
                                                                                     cacCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTgggg                                                                 <  1:3624218/150‑1 (MQ=255)
                                                                                         tGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCCAGCAGGCCCTGCAAGGGcagc                                                             <  2:3033862/150‑4 (MQ=255)
                                                                                           aTGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCtcgtc                                                           <  1:8406956/150‑1 (MQ=255)
                                                                                            tGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCtcgtcg                                                          >  1:3304400/1‑150 (MQ=255)
                                                                                              cAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTg                                                        >  2:2572515/1‑150 (MQ=255)
                                                                                               aGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTgg                                                       <  1:5043131/150‑1 (MQ=255)
                                                                                               aGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTgg                                                       <  2:489187/150‑1 (MQ=255)
                                                                                                gCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGc                                                      >  1:179917/1‑150 (MQ=255)
                                                                                                gCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGc                                                      >  2:666783/1‑150 (MQ=255)
                                                                                                 cGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCt                                                     <  2:4507005/150‑1 (MQ=255)
                                                                                                       tACGAACCGGAAAGCCTGCTTGAGGCGCAATTGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAg                                               >  2:1694889/1‑150 (MQ=255)
                                                                                                       tACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAg                                               >  2:2154446/1‑150 (MQ=255)
                                                                                                       tACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAg                                               >  1:340553/1‑150 (MQ=255)
                                                                                                       tACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAg                                               >  1:8721965/1‑150 (MQ=255)
                                                                                                        aCGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGc                                              >  2:70185/1‑150 (MQ=255)
                                                                                                        aCGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGc                                              >  1:1389334/1‑150 (MQ=255)
                                                                                                         cGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCa                                             <  1:2757380/150‑1 (MQ=255)
                                                                                                         cGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCa                                             <  2:5208139/150‑1 (MQ=255)
                                                                                                           aaCCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAgg                                           >  1:4334835/1‑150 (MQ=255)
                                                                                                                gAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGaa                                      >  2:3199270/1‑150 (MQ=255)
                                                                                                                   aGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATgc                                   <  1:1136107/150‑1 (MQ=255)
                                                                                                                   aGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATgc                                   >  1:4052597/1‑150 (MQ=255)
                                                                                                                     ccTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATgcgc                                 >  2:3351152/1‑150 (MQ=255)
                                                                                                                      cTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTTACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCa                                >  1:474871/1‑150 (MQ=255)
                                                                                                                      cTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCa                                >  2:2877185/1‑150 (MQ=255)
                                                                                                                         cTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACgg                             <  2:5607396/150‑1 (MQ=255)
                                                                                                                         cTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACgg                             <  2:4223090/150‑1 (MQ=255)
                                                                                                                           tGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCa                           <  2:4787505/150‑1 (MQ=255)
                                                                                                                            gAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAg                          >  1:7479064/1‑150 (MQ=255)
                                                                                                                               gcgcAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTg                       >  1:6807177/1‑150 (MQ=255)
                                                                                                                               gcgcAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTg                       >  1:5817884/1‑150 (MQ=255)
                                                                                                                                 gcAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGAt                     >  1:5429306/1‑150 (MQ=255)
                                                                                                                                  ccctcgCTGGCAAGCATGC‑‑‑‑‑‑‑‑‑‑CTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATc                    >  1:8102648/6‑150 (MQ=255)
                                                                                                                                   cctcgCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑CTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCg                   >  1:1027645/5‑150 (MQ=255)
                                                                                                                                   cctcgCTGGCAAGCATGC‑‑‑‑‑‑‑‑‑‑CTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCg                   >  1:3215023/5‑150 (MQ=255)
                                                                                                                                    ctcgCTGGCAAGCATGC‑‑‑‑‑‑‑‑‑‑CTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTgg                                                                   <  1:5265965/98‑1 (MQ=255)
                                                                                                                                       gCTGGCAAGCATGC‑‑‑‑‑‑‑‑‑‑CTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGc               >  2:7289328/1‑150 (MQ=255)
                                                                                                                                        cTGGCAAGCATGC‑‑‑‑‑‑‑‑‑‑CTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCg              >  2:7192566/1‑150 (MQ=255)
                                                                                                                                          ggcaggcaTGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTa            <  2:7944448/150‑1 (MQ=255)
                                                                                                                                          ggcaggcaTGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTa            <  1:1641904/150‑1 (MQ=255)
                                                                                                                                             aggcaTGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTACaa         >  2:6936947/1‑150 (MQ=255)
                                                                                                                                             aggcaTGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTACaa         >  1:6467883/1‑150 (MQ=255)
                                                                                                                                             aggcaTGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTACaa         >  1:3999565/1‑150 (MQ=255)
                                                                                                                                               gcaTGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTACAatg       <  2:5459558/150‑1 (MQ=255)
                                                                                                                                               gcaTGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTACAatg       <  2:881688/150‑1 (MQ=255)
                                                                                                                                                 aTGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTACAatgat     <  1:2494614/150‑1 (MQ=255)
                                                                                                                                                 aTGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTACAatgat     >  1:766000/1‑150 (MQ=255)
                                                                                                                                                  tGC‑‑‑‑‑‑‑‑‑‑GTGCCAGCGAGGGGATCGCTGTACACGTGGTCGGCCGTGCCCTTGTCGGTGCCGCCGGCGAGCGGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTACAatgatg    <  2:7649790/150‑1 (MQ=255)
                                                                                                                                                    c‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTACAATGATGcc  >  1:6108641/1‑150 (MQ=255)
                                                                                                                                                               |                                                                                                                                                    
ATTGCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGC‑‑‑‑‑‑‑‑‑‑TTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTACAATGATGCC  >  NC_002947/873279‑873576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑