BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,014,651	A→G	5.1%	W33R (TGG→CGG) ‡	SBRG_Ecoli_galT ←	SBRG_Ecoli_galT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,014,651	0	A	G	5.1%	2954.8 / 82.8	912	W33R (TGG→CGG) ‡	SBRG_Ecoli_galT	SBRG_Ecoli_galT
Reads supporting (aligned to +/- strand): ref base A (465/398); new base G (24/23); total (491/421)
Fisher's exact test for biased strand distribution p-value = 7.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.44e-01

Only 100 of 920 total aligned reads displayed.

CAAAGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTGGATGGCGTGACTGCCAGTGCCAGG  >  NC_002947/1014502‑1014800

 aaaGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAg                                                                                                                                                      >  2:4217826/1‑150 (MQ=255)

 aaaGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAg                                                                                                                                                      <  2:4041805/150‑1 (MQ=255)

  aaGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAgg                                                                                                                                                     >  1:6042246/1‑150 (MQ=255)

   aGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAggg                                                                                                                                                    <  1:1441109/150‑1 (MQ=255)

   aGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAggg                                                                                                                                                    <  2:3482359/150‑1 (MQ=255)

     ccGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGgc                                                                                                                                                  <  1:2291564/150‑1 (MQ=255)

     ccGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGgc                                                                                                                                                  <  1:6513129/150‑1 (MQ=255)

     ccGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGgc                                                                                                                                                  <  1:3318815/150‑1 (MQ=255)

      cGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGgcg                                                                                                                                                 >  1:370517/1‑150 (MQ=255)

       gCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGgcgc                                                                                                                                                >  1:2121523/1‑150 (MQ=255)

               aTTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGcgcg                                                                                                                                        >  2:4382682/1‑150 (MQ=255)

                    tGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCgcggtgcg                                                                                                                                   >  1:7690408/1‑150 (MQ=255)

                    tGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCgcggtgcg                                                                                                                                   <  1:8500486/150‑1 (MQ=255)

                    tGAAAACGTAAGTCCCGGTGTAATAGGGGATTTTATAGCCTGTCACCCGCACATTAACTGCGCAGAGGAAGAAATCTGGATCGAGCGAAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTCCCAGGGGCGCTTAGCgcggttcg                                                                                                                                   >  2:1935822/1‑150 (MQ=255)

                      aaaaCGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCgcggtgcggt                                                                                                                                 >  1:25558/1‑150 (MQ=255)

                      aaaaCGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCgcggtgcggt                                                                                                                                 >  2:7338353/1‑150 (MQ=255)

                        aaCGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGa                                                                                                                               <  2:7048554/150‑1 (MQ=255)

                           gTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAAc                                                                                                                            >  2:3253676/1‑150 (MQ=255)

                           gTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAAc                                                                                                                            >  1:5716442/1‑150 (MQ=255)

                           gTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAAc                                                                                                                            >  1:8608244/1‑150 (MQ=255)

                              aGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAg                                                                                                                         <  1:1760874/150‑1 (MQ=255)

                                tCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGTGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGaa                                                                                                                       >  1:1624811/1‑150 (MQ=255)

                                 cccGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAAt                                                                                                                      <  1:4204640/150‑1 (MQ=255)

                                  ccGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATc                                                                                                                     <  2:5740100/150‑1 (MQ=255)

                                   cGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATcc                                                                                                                    >  2:3734938/1‑150 (MQ=255)

                                   cGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATcc                                                                                                                    >  1:1160688/1‑150 (MQ=255)

                                    ggTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCa                                                                                                                   <  2:795491/150‑1 (MQ=255)

                                    ggTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCa                                                                                                                   <  1:641514/150‑1 (MQ=255)

                                    ggTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCa                                                                                                                   <  2:2999153/150‑1 (MQ=255)

                                    ggTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCa                                                                                                                   >  2:2708845/1‑150 (MQ=255)

                                      tgtAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCAtt                                                                                                                 >  2:104674/1‑150 (MQ=255)

                                       gtAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTg                                                                                                                <  1:6848008/150‑1 (MQ=255)

                                           tCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCg                                                                                                            >  1:2388566/1‑150 (MQ=255)

                                             ggggTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGt                                                                                                          <  1:4650417/150‑1 (MQ=255)

                                             ggggTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGt                                                                                                          >  1:3573938/1‑150 (MQ=255)

                                             ggggTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGt                                                                                                          >  2:6399042/1‑150 (MQ=255)

                                              gggTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTg                                                                                                         >  1:6684154/1‑150 (MQ=255)

                                              gggTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTg                                                                                                         >  2:7507182/1‑150 (MQ=255)

                                               ggTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGa                                                                                                        >  1:3448860/1‑150 (MQ=255)

                                                 tttttATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGc                                                                                                      <  1:8744226/150‑1 (MQ=255)

                                                     tATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGt                                                                                                  <  1:8162228/150‑1 (MQ=255)

                                                         gCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTa                                                                                              <  1:3139309/150‑1 (MQ=255)

                                                         gCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTa                                                                                              <  2:7755504/150‑1 (MQ=255)

                                                          ccTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGT‑AAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAgc                                                                                            >  2:3819308/1‑150 (MQ=255)

                                                           cTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAgc                                                                                            >  1:6215831/1‑150 (MQ=255)

                                                           cTGTCACCCGCACAGTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAgc                                                                                            <  2:6580112/150‑1 (MQ=255)

                                                             gTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAgcgg                                                                                          <  2:130832/150‑1 (MQ=255)

                                                              tCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAgcggc                                                                                         <  2:8584286/150‑1 (MQ=255)

                                                              tCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAgcggc                                                                                         >  2:4810557/1‑150 (MQ=255)

                                                                  ccGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTGGCGGCGAtg                                                                                     >  1:3837562/1‑150 (MQ=255)

                                                                  ccGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATAGCCCGGTGAGCGGGTTGTAGCGGCGAtg                                                                                     >  2:5242393/1‑150 (MQ=255)

                                                                    gCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGAtgtg                                                                                   <  2:2457324/150‑1 (MQ=255)

                                                                      acaTTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGga                                                                                 <  2:5874092/150‑1 (MQ=255)

                                                                       caTTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGgat                                                                                <  1:704908/150‑1 (MQ=255)

                                                                        aTTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGgatg                                                                               <  1:6930083/150‑1 (MQ=255)

                                                                             cTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCaa                                                                          >  2:6242392/1‑150 (MQ=255)

                                                                              tGCGCAGAGGAAGGAATCTGGATCGTGCGCTGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCTAc                                                                         <  2:8205158/150‑1 (MQ=255)

                                                                                cgcAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACgg                                                                       <  1:7963525/150‑1 (MQ=255)

                                                                                 gcAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACggg                                                                      <  2:6798405/150‑1 (MQ=255)

                                                                                      ggAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTaa                                                                 >  2:1662721/1‑150 (MQ=255)

                                                                                       gAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTaaa                                                                >  2:4947013/1‑150 (MQ=255)

                                                                                         aGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAAtt                                                              <  1:8409147/150‑1 (MQ=255)

                                                                                                    tCGTGCGCAGTTAACTCCTGTTTGTCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCTTTGCCCGGTGAGAGGGTTGTAGCGGCGATGTGGATGATCAACTGGTTTAAATTGCGTCATTGTc                                                   <  2:5529165/150‑1 (MQ=255)

                                                                                                    tCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTc                                                   >  2:2130231/1‑150 (MQ=255)

                                                                                                       tGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGtt                                                <  1:8047734/150‑1 (MQ=255)

                                                                                                        gcgcAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTc                                               <  2:689568/150‑1 (MQ=255)

                                                                                                         cgcAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTcc                                              <  1:1084651/150‑1 (MQ=255)

                                                                                                               tAACCCCTGTTTGGCTGGCGTGTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATc                                        <  2:7863845/150‑1 (MQ=255)

                                                                                                               tAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGACGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATc                                        <  2:845458/150‑1 (MQ=255)

                                                                                                                  cacCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCggg                                     <  1:7554226/150‑1 (MQ=255)

                                                                                                                   acCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGTATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGa                                    >  1:276441/1‑150 (MQ=255)

                                                                                                                   acCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGa                                    >  1:7361120/1‑150 (MQ=255)

                                                                                                                   acCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGa                                    >  1:5923647/1‑150 (MQ=255)

                                                                                                                     cTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGata                                  >  1:5395358/1‑150 (MQ=255)

                                                                                                                      tGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGatat                                 <  2:1805435/150‑1 (MQ=255)

                                                                                                                        tttGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATcc                               >  1:4999548/1‑150 (MQ=255)

                                                                                                                           ggctggcGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCtg                            <  2:4500987/150‑1 (MQ=255)

                                                                                                                              tggcGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCCTGGTCGTTCCTTAATCGGGATATCCCTGTgg                         >  1:2943595/1‑150 (MQ=255)

                                                                                                                              tggcGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTgg                         >  1:7883329/1‑150 (MQ=255)

                                                                                                                              tggcGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTgg                         >  1:5582153/1‑150 (MQ=255)

                                                                                                                              tggcGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTgg                         >  2:8077168/1‑150 (MQ=255)

                                                                                                                               ggcGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTGGa                        >  2:3084487/1‑150 (MQ=255)

                                                                                                                                  gTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTCGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTGGATgg                     >  2:6024665/1‑150 (MQ=255)

                                                                                                                                  gTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTGGATgg                     >  2:5393269/1‑150 (MQ=255)

                                                                                                                                  gTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTGGATgg                     >  2:3918569/1‑150 (MQ=255)

                                                                                                                                  gTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTGGATgg                     <  1:4824970/150‑1 (MQ=255)

                                                                                                                                      taagcgcCCCTGGCAGGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGtt                                                >  1:959252/4‑119 (MQ=255)

                                                                                                                                      taagcgcCCCTGGCAGGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTGGATGGCGTg                 >  1:4099889/4‑150 (MQ=255)

                                                                                                                                      taagcgcCCCTGGCAGGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTGGATGGCGTg                 >  2:1478703/4‑150 (MQ=255)

                                                                                                                                       cTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTGGATGGCGTGa                >  2:881106/1‑150 (MQ=255)

                                                                                                                                       cTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGGGCGGTGAAACCAGAATCCATTGCCCGGGGAGCGGGGTGTAGCGGCGATGTGGCGGATCAACGGGATTAAATTGCGGCAGGGTCGTTCCTTCATCGGGATATCCCGGTGGCTGGCGTgc                >  2:2223911/1‑149 (MQ=255)

                                                                                                                                        tGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTGGATGGCGTGAc               >  2:5140655/1‑150 (MQ=255)

                                                                                                                                         gcgcCCCTGGCAGGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTGGATGGCGTGACt              >  2:2565276/1‑150 (MQ=255)

                                                                                                                                            cccccTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTGGATGGCGTGACtgcc           >  2:2798513/1‑150 (MQ=255)

                                                                                                                                             ccccTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTGGATGGCGTGACtgcca          >  1:4565388/1‑150 (MQ=255)

                                                                                                                                             ccccTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTGGATGGCGTGACtgcca          <  2:547092/150‑1 (MQ=255)

                                                                                                                                              cccTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTGGATGGCGTGACtgccag         >  1:1919478/1‑150 (MQ=255)

                                                                                                                                               ccTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTGGATGGCGTGACtgccagt        >  1:4435475/1‑150 (MQ=255)

                                                                                                                                                   ccAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTGGATGGCGTGACtgccagtgcca    >  2:8346490/1‑150 (MQ=255)

                                                                                                                                                    cAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTGGATGGCGTGACtgccagtgccag   <  2:4619865/150‑1 (MQ=255)

CAAAGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGCACATTACCTGCGCAGAGGAAGCAATCTGGATCGTGCGCAGGTAACACCTGTTTGGCTGGCGTTTCCTGCGCCCCCTGCCAGGGGCGCTTAGCGCGGTGCGGTGAAACCAGAATCCATTGCCCGGTGAGCGGGTTGTAGCGGCGATGTGGATGATCAACGGGATTAAATTGCGTCATGGTCGTTCCTTAATCGGGATATCCCTGTGGATGGCGTGACTGCCAGTGCCAGG  >  NC_002947/1014502‑1014800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`