Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,107,605 A→C 7.7% Q31H (CAA→CAC)  ttg2E → toluene‑tolerance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,107,6050AC 7.7% 1312.9 / 7.7 458Q31H (CAA→CAC) ttg2Etoluene‑tolerance protein
Reads supporting (aligned to +/- strand):  ref base A (294/86);  new base C (2/46);  total (296/162)
Fisher's exact test for biased strand distribution p-value = 3.49e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 545 total aligned reads displayed.

CGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGCCACTGGCAAGGCGTGCGAGGTGCGTGGCAT  >  NC_002947/1107456‑1107754
                                                                                                                                                     |                                                                                                                                                     
cGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCgcaagcaa                                                                                                                                                       <  1:1392448/150‑1 (MQ=255)
    gAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCCTGCGCAAgcaaggca                                                                                                                                                   <  1:8413157/150‑1 (MQ=255)
      aGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAgcaaggcaag                                                                                                                                                 <  2:6878643/150‑1 (MQ=255)
           ccaaggccaagCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCt                                                                                                                                            >  1:6801080/1‑150 (MQ=255)
            caaggccaagCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTg                                                                                                                                           >  2:8784266/1‑150 (MQ=255)
             aaggccaagCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGa                                                                                                                                          >  1:3067927/1‑150 (MQ=255)
              aggccaagCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGAt                                                                                                                                         >  1:8007920/1‑150 (MQ=255)
               ggccaagCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATc                                                                                                                                        >  1:2900393/1‑150 (MQ=255)
                  caagCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCGGATCGAt                                                                                                                                     >  1:6443975/1‑150 (MQ=255)
                         gCTGCCGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTcgc                                                                                                                              <  1:3329339/150‑1 (MQ=255)
                         gCTGCCGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTcgc                                                                                                                              >  2:6087594/1‑150 (MQ=255)
                         gCTGCCGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGAGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCTATGGCTcgc                                                                                                                              >  1:7731163/1‑150 (MQ=255)
                           tGCCGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTcgcgc                                                                                                                            >  1:483551/1‑150 (MQ=255)
                            gCCGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTcgcgcg                                                                                                                           <  1:7535197/150‑1 (MQ=255)
                             ccGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGa                                                                                                                          <  1:4397875/150‑1 (MQ=255)
                             ccGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGa                                                                                                                          >  2:5768773/1‑150 (MQ=255)
                              cGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAg                                                                                                                         <  1:5476147/150‑1 (MQ=255)
                              cGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAg                                                                                                                         >  2:3510748/1‑150 (MQ=255)
                               gACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGc                                                                                                                        >  2:4764085/1‑150 (MQ=255)
                                  aaCTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGTACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGccat                                                                                                                     <  1:1092884/150‑3 (MQ=255)
                                  aaCTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGccat                                                                                                                     <  2:159842/150‑3 (MQ=255)
                                  aaCTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCcgc                                                                                                                     >  1:3420136/1‑150 (MQ=255)
                                   aCTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGTGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCcgcg                                                                                                                    >  2:7908110/1‑150 (MQ=255)
                                   aCTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAgttgtc                                                                                                                    <  2:8042797/150‑6 (MQ=255)
                                   aCTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGccatc                                                                                                                    <  1:2307185/150‑4 (MQ=255)
                                    cTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGa                                                                                                                   >  2:2777751/1‑150 (MQ=255)
                                     tCGCCAGAGAAGAGCGTCAAATGAGTGAGGCAGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGAc                                                                                                                  >  1:2105836/1‑150 (MQ=255)
                                       gCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTg                                                                                                                >  1:6599972/1‑150 (MQ=255)
                                         cAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGt                                                                                                              >  1:7278247/1‑150 (MQ=255)
                                               agagCGTCAATTGAGTGAGGCCGCTGTAAGCATGGCAGAGCCTGGGGTGCTGCGTGTGACTGGGGTGCTGGACTACCGCTGGGGCCGGGCCTTGCGCAAGCAAGGCATGGTGCTTATCGTGGGCGCGCGCGCGCCGCGACTGGTGCTTGa                                                                                                        <  2:1987802/150‑1 (MQ=255)
                                                    gaaaaaTGTGTGTGGCCGCTGTATGCTTGGCAGAGCCTGGGGTGCTGCGTGGGACTGGGGTGGTGGACTCCCGCCGCGGCCGGGCCTTGCGAACGCACGGCATGGTGCTGAGCGCGGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCt                                                                                                   <  2:6480387/147‑1 (MQ=255)
                                                    gTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCt                                                                                                   >  2:762547/1‑150 (MQ=255)
                                                    gTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCt                                                                                                   >  2:8274134/1‑150 (MQ=255)
                                                      cAAATGCGTGTGGCCGCTGTACGCTTGGCAGCGCCTGGCGTGCTGGGTCTGAGGGGGGTGGTGGACTCCCGCCGGGGCCGGGCCTTGCGCACGCACGGCTGGGTGCTGATGGTGGGCTCGCGCGCGCCGCGACTGGTGCTTGATTGCTCg                                                                                                 <  2:1203248/150‑1 (MQ=255)
                                                       acaTGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCgg                                                                                                >  2:1517905/3‑150 (MQ=255)
                                                       aaaTGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGAGTGCTCgg                                                                                                >  2:7160826/1‑150 (MQ=255)
                                                        aaTGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGc                                                                                               >  2:5982880/1‑150 (MQ=255)
                                                            tgtgcgGCCGCTGTAAGATTGGCCGCGCCTGGGGTGCTGCGTCTGCCTGGGGTGCTGGACTCCCGCCGCGGCCGGGCCTTGCGCACGCACGGCACGGTGGTGAGCGTTGGCTCGCGCGCGCCGCGACTGGTGCTTGATTGCTCGGCTGTc                                                                                           <  2:4371416/149‑1 (MQ=255)
                                                             gtgagGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCg                                                                                          >  1:5919438/1‑150 (MQ=255)
                                                             gtgagGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCg                                                                                          >  1:6468683/1‑150 (MQ=255)
                                                                  gCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAg                                                                                     >  1:266956/1‑150 (MQ=255)
                                                                  gCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAg                                                                                     >  1:6139121/1‑150 (MQ=255)
                                                                     gCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAgtcg                                                                                  >  2:4016568/1‑150 (MQ=255)
                                                                       tGTACGCTTGGCAGAGCCAGGCGTGCGGCGTCGGAGTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTGGCGCAAGCAAGGCACGTTGCTGATCGTGGGCGCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAgtcgtc                                                                                <  2:6442653/150‑1 (MQ=255)
                                                                       tGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAgtcgtc                                                                                >  1:8597787/1‑150 (MQ=255)
                                                                           aGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAgt                                                                            >  2:7409880/1‑150 (MQ=255)
                                                                            gCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAgtg                                                                           >  1:6007129/1‑150 (MQ=255)
                                                                             cATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAgtgt                                                                          >  1:3196496/1‑150 (MQ=255)
                                                                               gggCCGAGCCTGGGGTGCTGCGTCTGAGTGGCGTGCGGGACTACCGCCGCGGCCCGGCCTTGCGAAAGCATGGCACGGTGCTGATCTTTGGCCCGCGCGCGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTg                                                                        <  2:4641107/149‑1 (MQ=255)
                                                                                 gCAGAGCCTGGGGTGCTGCGTCTGACTGGGGTGGTGGACTACCGCCGCGGCCCGGCGTTGCGCAAGAATGGCATGGTGCTGATCGTTGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGc                                                                      <  2:489923/150‑1 (MQ=255)
                                                                                 gCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGCCTACCGCAGCGGCCCGGCCTTGCGCAAGCACGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGCCTGGTGCTTGATTGCTCGGCTGTCGAGAAGGCGTCGAGTGTTGGc                                                                      >  2:1787602/1‑150 (MQ=255)
                                                                                  cAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTggtc                                                                     >  1:8158027/1‑148 (MQ=255)
                                                                                  cAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGcc                                                                     >  1:2506635/1‑150 (MQ=255)
                                                                                        cAGGCTTGCTGCGTCTGCCGGGCGTGCTGCACTACCCCCGCGGCCCGGACTTCCCCACGCACGGCATGGTGCTTATCCATGGCCCGCGCGACCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGc                                                               <  2:1833382/150‑1 (MQ=255)
                                                                                         aGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCt                                                              >  1:4658602/1‑150 (MQ=255)
                                                                                         aGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCt                                                              >  1:3738686/1‑150 (MQ=255)
                                                                                           gCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTAc                                                            >  1:5658665/1‑150 (MQ=255)
                                                                                             gtggtgCGTCTGCGTGGGGTGCTGGACTCCCGCCGGGGCCCGGCCTGGCGAACGCACGGCATGGTGCTTAGCGGTGGCGCGCGCGCCCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTActg                                                          <  1:7156295/150‑1 (MQ=255)
                                                                                             gTGCTGCGTCTGCCTGGCGTGCTGGACTACCGCTGCGGCCGGGCCTTGCGCAAGCACGGCAAGGTGCTGATCGTTGGCGCGCGCGACCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTActg                                                          <  2:535355/150‑1 (MQ=255)
                                                                                                  ggttCTGCCGGGGGTGCGGGACTCCCCCCGGGGCCCGGCCTGGCGAACGAAGGGCATGGTGCTGAGGTTGGGCTGGCGGGACCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGc                                                     <  2:2662283/147‑1 (MQ=255)
                                                                                                  gcGTCTGCCTGGCGTGCTGGACTACCGCCGGGGCCCGGCCTGGCGCACGCATGGCATGGTGCTGATCGCGGGCCCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGc                                                     <  2:593198/150‑1 (MQ=255)
                                                                                                    gTCTGACTGGCGTGCTGGACTACCACAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGt                                                   >  2:3917498/1‑150 (MQ=255)
                                                                                                        gACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACAt                                               >  1:8781206/1‑150 (MQ=255)
                                                                                                        gACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACAt                                               >  1:7831904/1‑150 (MQ=255)
                                                                                                         aCTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATg                                              >  1:6528996/1‑150 (MQ=255)
                                                                                                           tGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCg                                            >  2:2221161/1‑150 (MQ=255)
                                                                                                           tGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCg                                            >  2:7787828/1‑150 (MQ=255)
                                                                                                           tGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCg                                            >  1:4284368/1‑150 (MQ=255)
                                                                                                           tGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCg                                            >  1:7422998/1‑150 (MQ=255)
                                                                                                             gCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCggg                                          >  1:1219993/1‑150 (MQ=255)
                                                                                                                   tGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTc                                    >  2:6675949/1‑150 (MQ=255)
                                                                                                                   tGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTc                                    >  1:7032677/1‑150 (MQ=255)
                                                                                                                   tGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTc                                    >  1:8118789/1‑150 (MQ=255)
                                                                                                                   ggcaCTCCCGCCGGGGCCCGGCCTTGCCCACCCACGGCTTGGTGTTGATGGCGGGCGCGCGCGCCCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTc                                    <  2:3155435/147‑1 (MQ=255)
                                                                                                                    ggACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCa                                   >  2:3765749/1‑150 (MQ=255)
                                                                                                                       cccccgccgGGGCCGGGCGTTGCGCACGCTCGGCCTGGTGCTGCGCGTGGGCGCGCGCGCCCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGc                                <  1:2001877/147‑1 (MQ=255)
                                                                                                                        tACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGcc                               >  1:5098076/1‑150 (MQ=255)
                                                                                                                         aCCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGCCa                              >  1:4345414/1‑150 (MQ=255)
                                                                                                                            gccgCGGCCCGGCCTGGCCCCAGCCCGCCATGGTGGTGCTGGTGGGCCCGCGCGCCCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGCCACTg                           <  2:2914787/147‑1 (MQ=255)
                                                                                                                               gCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGCTGCTCAGGCCACTGGCa                        >  2:6222560/1‑150 (MQ=255)
                                                                                                                                cggcccggccGGGCGCAAGCTAGGCTTGGTGCTGCTGGTTGGCTGGCGCGCGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGCCACTGGCaa                       <  1:5847602/150‑1 (MQ=255)
                                                                                                                                 ggcccggcgTGGCGCAAGCACGGCATGGGGCGGATCGTGGGCGCGCGCGCGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGCCACTGGCAAg                      <  2:6528169/150‑1 (MQ=255)
                                                                                                                                 ggcccggccGGGCCCAACCACGGCTCGGTGGTGATGGTGGGCCCGCGCGCCCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGCCACTGGCAAg                      <  2:1103763/150‑1 (MQ=255)
                                                                                                                                  gcccggccTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGCCACTGGCAAgg                     >  1:1651091/1‑150 (MQ=255)
                                                                                                                                   cggggccTTGCCCAAGCTCGTCTTGGTGCTGTGCGTGGGGGCGCGCGCCCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGCCACTGGCAAGGc                    <  2:3296849/147‑1 (MQ=255)
                                                                                                                                   cccggccTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCAGCGACTGGTGCTTGAATGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGCCACTGGCAAGGc                    >  2:3664704/1‑150 (MQ=255)
                                                                                                                                   cccggccTGGCTCAAGAATGGCATGGTGATTATCTTTGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGCCACTGGCAAGGc                    <  2:5005690/150‑1 (MQ=255)
                                                                                                                                       gcgttGCGCACGCACGGCACGGTGTTGTTCGATGGCTCGCGCTCGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGCCACTGGCAAGGCGTGc                <  2:1350874/147‑1 (MQ=255)
                                                                                                                                       gccTTGCGCACTCACGGCTCGGTGCTGAGCGTGGGCCCGCGCGCCCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGCCACTGGCAAGGCGTGc                <  2:1020708/150‑1 (MQ=255)
                                                                                                                                         cTTGCGCACGCACGGCTTGGTGCTGACGGAGGGCCCGGGCGCCCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGCCACTGGCAAGGCGTGCGa              <  2:8452614/150‑1 (MQ=255)
                                                                                                                                          ttGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGCCACTGGCAAGGCGTGCGAg             >  1:5300117/1‑150 (MQ=255)
                                                                                                                                          ttGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGCCACTGGCAAGGCGTGCGAg             >  1:1821835/1‑150 (MQ=255)
                                                                                                                                              gcaagcaaGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGCCACTGGCAAGGCGTGCGAGGTGc         >  2:5095923/1‑150 (MQ=255)
                                                                                                                                                aagcacgGCTCGGTGCTTTTCGTTGGCGCCCGCCCCCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGCCACTGGCAAGGCGTGCGAGGTGCGt       <  1:3603629/150‑1 (MQ=255)
                                                                                                                                                aagcacgGCATGGTGCTTATCGTTGGCTCGCGCGCGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGCCACTGGCAAGGCGTGCGAGGTGCGt       <  2:3446392/150‑1 (MQ=255)
                                                                                                                                                aagcaagGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATACTCAGGCCACTGGCAAGGCGTGCGAGGTGCGt       >  1:4181346/1‑150 (MQ=255)
                                                                                                                                                 agcaagGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGTGTACATGCGGGATCCTCAGGCCACTGGCAAGGCGTGCGAGGTGCGTg      >  2:5541308/1‑150 (MQ=255)
                                                                                                                                                  gcaaggcaagGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGCCACTGGCAAGGCGTGCGAGGTGCGTgg     >  1:4933941/1‑150 (MQ=255)
                                                                                                                                                  gcaaggcaagGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGCCACTGGCAAGGCGTGCGAGGTGCGTgg     >  2:8681280/1‑150 (MQ=255)
                                                                                                                                                     aggcaaggTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGCCACTGGCAAGGCGTGCGAGGTGCGTGGCAt  >  2:4211592/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGGCGAAGTGGCCAAGGCCAAGCAGGCTGCCGACAACTCGCCAGAGAAGAGCGTCAAATGAGTGAGGCCGCTGTAAGCATGGCAGAGCCAGGCGTGCTGCGTCTGACTGGCGTGCTGGACTACCGCAGCGGCCCGGCCTTGCGCAAGCAAGGCAAGGTGCTGATCGATGGCTCGCGCGAGCCGCGACTGGTGCTTGATTGCTCGGCTGTCGAGAAGTCGTCGAGTGTTGGCCTGTCGCTACTGCTGGCGTACATGCGGGATGCTCAGGCCACTGGCAAGGCGTGCGAGGTGCGTGGCAT  >  NC_002947/1107456‑1107754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑