Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,206,940 A→C 8.6% Y130S (TAC→TCC)  xcpW → type II secretion pathway protein XcpW

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,206,9400AC 8.6% 1963.0 / 31.2 648Y130S (TAC→TCC) xcpWtype II secretion pathway protein XcpW
Reads supporting (aligned to +/- strand):  ref base A (397/175);  new base C (3/73);  total (400/248)
Fisher's exact test for biased strand distribution p-value = 4.28e-30
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 667 total aligned reads displayed.

GCTGGACTGGCAGCCCGCCAGCAACCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCGGTGCACCCAGGTCGTGGTGTGCGTCTGGAACTGCTGC  >  NC_002947/1206791‑1207089
                                                                                                                                                     |                                                                                                                                                     
gCTGGACTGGCAGCCCGCCAGCAACCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTa                                                                                                                                                       <  1:141986/150‑1 (MQ=255)
gCTGGACTGGCAGCCCGCCAGCAACCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTa                                                                                                                                                       >  1:7311170/1‑150 (MQ=255)
  tGGACTGGCAGCCCGCCAGCACCCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTAcg                                                                                                                                                     >  1:3443156/1‑150 (MQ=255)
  tGGACTGGCAGCCCGCCAGCAACCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTAcg                                                                                                                                                     >  1:2226969/1‑150 (MQ=255)
   ggACTGGCAGCCCGCCAGCAACCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTAcgc                                                                                                                                                    >  2:4689009/1‑150 (MQ=255)
    gACTGGCAGCCCGCCAGCAACCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTAcgcc                                                                                                                                                   >  1:8495436/1‑150 (MQ=255)
    gACTGGCAGCCCGCCAGCAACCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTAcgcc                                                                                                                                                   >  1:3070413/1‑150 (MQ=255)
       tGGCAGCCCGCCAGCAACCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGTACAGGTccgccgag                                                                                                                                                <  2:3501150/150‑3 (MQ=255)
       tGGCAGCCCGCCAGCAACCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCt                                                                                                                                                >  1:1691932/1‑150 (MQ=255)
           aGCCCGCCAGCAACCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGgcg                                                                                                                                            >  1:2372996/1‑150 (MQ=255)
           aGCCCGCCAGCAACCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGgcg                                                                                                                                            >  1:155607/1‑150 (MQ=255)
              ccGCCAGCAACCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGccc                                                                                                                                         >  1:2800507/1‑150 (MQ=255)
               cGCCAGCAACCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGcccc                                                                                                                                        >  2:2131123/1‑150 (MQ=255)
               cGCCAGCAACCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGcccc                                                                                                                                        >  2:7464627/1‑150 (MQ=255)
               cGCCAGCAACCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGcccc                                                                                                                                        <  1:3966546/150‑1 (MQ=255)
                gCCAGCAACCAGTTGCTGTGGGTCGCTAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCcg                                                                                                                                       >  1:7756894/1‑150 (MQ=255)
                 ccAGCAACCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCcgc                                                                                                                                      >  1:7656540/1‑150 (MQ=255)
                 acAGCAACTGGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCcgc                                                                                                                                      >  1:5797564/2‑150 (MQ=255)
                 acAGCAACTGGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCcgc                                                                                                                                      >  2:6310389/2‑150 (MQ=255)
                   aGCAACCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCgcgc                                                                                                                                    >  1:4556436/1‑150 (MQ=255)
                   aGCAACCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCgcgc                                                                                                                                    >  1:3508376/1‑150 (MQ=255)
                    gCAACCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCt                                                                                                                                   <  2:8029530/150‑1 (MQ=255)
                    gCAACCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCt                                                                                                                                   >  1:3189918/1‑150 (MQ=255)
                     cAACCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTg                                                                                                                                  >  2:8714992/1‑150 (MQ=255)
                      aaCCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTgg                                                                                                                                 >  2:2398607/1‑150 (MQ=255)
                      aaCCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTgg                                                                                                                                 <  2:2113480/150‑1 (MQ=255)
                         cAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGATCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGtc                                                                                                                              >  1:6582766/1‑149 (MQ=255)
                            ttGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAg                                                                                                                           <  2:460036/150‑1 (MQ=255)
                            ttGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAAGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAg                                                                                                                           >  1:7090616/1‑150 (MQ=255)
                              gCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAgcg                                                                                                                         >  2:1855799/1‑150 (MQ=255)
                                 gtgGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGa                                                                                                                      <  1:4945953/150‑1 (MQ=255)
                                  tgGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGaa                                                                                                                     <  1:6396141/150‑1 (MQ=255)
                                  tgGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGaa                                                                                                                     >  2:2686313/1‑150 (MQ=255)
                                   gggTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAAc                                                                                                                    <  2:560272/150‑1 (MQ=255)
                                    ggTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACg                                                                                                                   >  1:93664/1‑150 (MQ=255)
                                      tCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGAc                                                                                                                 >  2:8184606/1‑150 (MQ=255)
                                      tCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGAc                                                                                                                 >  1:1882762/1‑150 (MQ=255)
                                         ccAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCg                                                                                                              >  1:5986385/1‑150 (MQ=255)
                                         ccAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCg                                                                                                              >  1:804508/1‑150 (MQ=255)
                                          cAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGa                                                                                                             >  2:6073285/1‑150 (MQ=255)
                                          cAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGa                                                                                                             >  2:7174127/1‑150 (MQ=255)
                                          cAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGa                                                                                                             >  2:2960685/1‑150 (MQ=255)
                                            gccccGGCCTGCGGGCTGCCGTTGGTGGGTGGGGCCTGCAACGCCTGGGCTTGGGTTTCGTGGCCGGGTTGAAACGCCTGGTGCTGTAACGCTGGGGGTACTTTTCGGCCGCTGGCGCCCCGGGTTGGGTACAGCGCGAACGACTCGAcc                                                                                                           <  2:6552706/147‑1 (MQ=255)
                                             cccgggCCTGCGGGCCGCCGTCGGTGGCTCGCGCGTGCACCGCCCGGGCCTGGGTTTCGAGGCCCGCGAGCAACGCGTGCTGGTGTAATGGTGGGGGGACGTTTACGCCGCGGGCGCCCGGGGGTGGTTACAGCGGGAACGACTCGACCa                                                                                                          <  2:4271103/146‑1 (MQ=255)
                                                cGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGt                                                                                                       >  1:5226729/1‑150 (MQ=255)
                                                    cTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAgc                                                                                                   >  1:6236509/1‑150 (MQ=255)
                                                     tGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAgcg                                                                                                  >  1:7847204/1‑150 (MQ=255)
                                                             gcggatggTGGCTCGCGCCTGCACCGCCAGCGCGTGGGTTTCGCGGCCCGCGACAAAGGCCTGCTGCTTTAACGCTGGGGGGACGTTTAGGCCGCTGGCGCCCCGCGCTGGTAACCGCGCGAACGACTCGACCAGGTCAGCGCCATGAAc                                                                                          <  2:1063072/144‑1 (MQ=255)
                                                               cGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACgt                                                                                        >  1:5654132/1‑150 (MQ=255)
                                                               cGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACgt                                                                                        >  2:7909611/1‑150 (MQ=255)
                                                               cGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACgt                                                                                        >  1:8183873/1‑150 (MQ=255)
                                                               cGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACgt                                                                                        >  1:4625029/1‑150 (MQ=255)
                                                                gAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACgtt                                                                                       >  1:543026/1‑150 (MQ=255)
                                                                 ttggTGGCCCGGGCCTGAACCGCCCGGGCGTGGGGTTCGCGGCCGGCGAGCAAGGCGTGCTGGTGTACCGCGGGGGGGAGCTGTCCGCCGCGGGCGCCCGGGGTTGTTTACAGGGCCAACGACTCGACCAGGTCAGCGCCATGAACgttc                                                                                      <  2:3236246/148‑1 (MQ=255)
                                                                  tgggggCTCGCCCCTCCACCGCCAGCGCGGGGGGTTCGCGCCCCGCGACCAACGCCTGCTGGTGGAACGCGGGGCGCACCTGAACGCCGGGGGCGCCCCGGGCTGGTAACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACgttct                                                                                     <  2:6680901/146‑1 (MQ=255)
                                                                     tGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACgttctgtt                                                                                  >  2:7273309/1‑150 (MQ=255)
                                                                     gggCTCGCGCCTCCACCGCCCGCGCCTGCGGTTGGTGGCCCGGGCGCAACGCCTGGTGCTGGAACGCTCGGCGGACGTGTACGCCGCTGGCGCCCCGCGGTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACgttctgtt                                                                                  <  2:6441281/149‑1 (MQ=255)
                                                                      ggCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACgttctgttc                                                                                 >  2:8555313/1‑150 (MQ=255)
                                                                        cTCGCGCCTGCAACGCCCGCGCCTGGGGTTCGCGGCCCGCGAGCAACGCCTGGTGGTGGAAAGCTGGGCGGACCTGTCCGCCGCGGGCGCCCCGCGGTGGGTACCGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTa                                                                               <  2:7050814/150‑1 (MQ=255)
                                                                         tCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTAc                                                                              >  2:6809160/1‑150 (MQ=255)
                                                                         tCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTAc                                                                              >  2:1717291/1‑150 (MQ=255)
                                                                           gcgcCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCa                                                                            >  1:5055514/1‑150 (MQ=255)
                                                                             gcCTGCAACGCCTGGGCCGGGGGTTGGCGGCCCGCTTGCACCCCTTGCTGGTGTATAGCTGGGGGTCCCTGTACGCCGCTGGCGCCCGCCGGGGGTTACCGCGCCAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAgg                                                                          <  2:5017075/150‑1 (MQ=255)
                                                                                 gccccGCCCGCGCCTGCGGTTCGCGCCCCGCGCGCACGGCCTGGTGGTGTAATGCTCGGCGGACCTGTCCGCCGCGGGCGCCCGGGGGTGGTTACGGCGCGCACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGggcgg                                                                      <  2:6923126/146‑1 (MQ=255)
                                                                                   aaCGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGcc                                                                    >  1:83125/1‑150 (MQ=255)
                                                                                    aCGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCg                                                                   >  2:3843022/1‑150 (MQ=255)
                                                                                      gCCTGCGCGTGGGGTGCGCGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGGGGACCTGTACGCCGGGGGCGCCCCGCGGTGTTAACCGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCgct                                                                 <  2:4441457/150‑1 (MQ=255)
                                                                                        cAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCgctgg                                                               >  2:7592640/1‑150 (MQ=255)
                                                                                        cAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCgctgg                                                               >  1:531750/1‑150 (MQ=255)
                                                                                          gcgcCGGGGGTTGGCGGCCCGGGAGCACGGCGTGGTGCTGCAACGCGCGGGGCACGTGTCCGCCGCGGGCGCCCGGCGGTGGGTCCCGGGCCAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCgctggct                                                             <  2:449661/150‑1 (MQ=255)
                                                                                           cgcCTGCGGTGCGCGGCCCGGTACCAACCCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCGGGCGCCCCGCGGGGGGTCCTGCGCGCACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCgctggctg                                                            <  1:8668531/150‑1 (MQ=255)
                                                                                             ccggCGTTTCGAGGCCGGGTTGCAACGCGTGGTGCTGGAATGCTGGGCGGAGGTGTACGCCGGGGGCGCCCGGGGGTGTTTACTGCGCGCACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCtggctggc                                                          <  2:1962816/147‑1 (MQ=255)
                                                                                              cTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGctggcc                                                         >  2:5777108/1‑150 (MQ=255)
                                                                                                gCGGTTCGCGGCCCGCGCCCACCGCCTGCGGCTGGAACGCGCGGGGGACGTGCCCGCCGCGGGCGCCCCGCGGGGGGCCCCGGGCGCACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGctggcctg                                                       <  2:8298454/150‑1 (MQ=255)
                                                                                                  ggTTCGCGGCCCGCCAGCACCGCCTGCGGCTGTACCGCGGGGGGGCCGTGTCCGCGGCGGGCGCCCGGGGCTGGTTACCCCGCGCACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGctggcctggc                                                     <  2:5973767/150‑1 (MQ=255)
                                                                                                              gcgAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAAc                                         >  2:5149851/1‑150 (MQ=255)
                                                                                                               cgAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTCCGCCGCTGGCGCCCCGCGCTGGGTACCGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAAcc                                        <  2:7988604/150‑1 (MQ=255)
                                                                                                                gagaaccGCCTGGTGCGGGAATGGGGGGCGGACCTGTCCGCGGGGGGCGCCCCGGGGTGGGTACCGCGCGCACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCg                                       <  1:7915849/144‑1 (MQ=255)
                                                                                                                gAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCg                                       >  1:2063418/1‑150 (MQ=255)
                                                                                                                     aCGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCGGTGCa                                  >  1:2741616/1‑150 (MQ=255)
                                                                                                                          tgctgctgGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCGGTGCACCCAg                             >  1:3751958/1‑150 (MQ=255)
                                                                                                                             tgctgGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCGGTGCACCCAGGTc                          >  2:3672119/1‑150 (MQ=255)
                                                                                                                               ctgGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCGGTGCACCCAGGTCgt                        >  1:1016908/1‑150 (MQ=255)
                                                                                                                                tgGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACTGGTGCACCCAGGTCgtg                       >  1:4253774/1‑150 (MQ=255)
                                                                                                                                tgGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCGGTGCACCCAGGTCgtg                       >  2:3336981/1‑150 (MQ=255)
                                                                                                                                 ggAAAGCTGGGGGGCCCTGTCCGCCGCGGGCGCCCCGCGCTGGGTACAGCGCGCACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCGGTGCACCCAGGTCgtgg                      <  2:4923597/150‑1 (MQ=255)
                                                                                                                                  gAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCGGTGCACCCAGGTCgtggt                     >  1:1224875/1‑150 (MQ=255)
                                                                                                                                  gAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCGGTGCACCCAGGTCgtggt                     >  1:6871561/1‑150 (MQ=255)
                                                                                                                                    atggtCGGCGTACCTGTACGCCGCTGGCGCCCCGCGGTGGTTACAGGGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCGGTGCACCCAGGTCGTGgtgt                   <  2:5677946/146‑1 (MQ=255)
                                                                                                                                    acgCTCGGGGCACCTGACGGCCGCGGGCGCCCGGGGGTGGTTACCGCGCGCACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCGGTGCACCCAGGTCGTGgtgt                   <  2:1507300/148‑1 (MQ=255)
                                                                                                                                    acgCTCGGCGTACCTGTACGCCGCGGGCGCCCCGCGGTGGGTACTGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCGGTGCACCCAGGTCGTGgtgt                   <  2:539097/148‑1 (MQ=255)
                                                                                                                                     ccagcggcgtACAGGTCCGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCGGTGCACCCAGGTCGTGGtgtg                  >  2:1306257/5‑150 (MQ=255)
                                                                                                                                       cTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCGGTGCACCCAGGTCGTGGTGTGCg                >  2:6862855/1‑150 (MQ=255)
                                                                                                                                         ggggggACGTTTACGCGGGTGGCGCCCCGCGTTGGTTACTGCGCTAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCGGTGCACCCAGGTCGTGGTGTGCGTc              <  2:7708734/146‑1 (MQ=255)
                                                                                                                                         cggcggACCTGTACGCCGCGGGCGCCCCGGGGTGGGTACAGCGCGCACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCCGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCGGTGCACCCAGGTCGTGGTGTGCGTc              <  2:1427310/150‑1 (MQ=255)
                                                                                                                                           ggggACCTTTACGCGGGGGGCGCCCCGCGGTGGTTCCAGCGCCCACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCGGTGCACCCAGGTCGTGGTGTGCGTCTg            <  2:3978251/148‑1 (MQ=255)
                                                                                                                                             ggACCTGTACGCCGCTGGCGCCCCGGGGGGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCGGTGCACCCAGGTCGTGGTGTGCGTCTGGa          <  2:521058/150‑1 (MQ=255)
                                                                                                                                               aCCTGTCCGCCGCGGGCCCCCCGCGGTGGTTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCGGTGCACCCAGGTCGTGGTGTGCGTCTGGAAc        <  1:6504674/150‑1 (MQ=255)
                                                                                                                                                ccTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCGGTGCACCCAGGTCGTGGTGTGCGTCTGGAAct       >  1:7161704/1‑150 (MQ=255)
                                                                                                                                                     aCGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCGGTGCACCCAGGTCGTGGTGTGCGTCTGGAACtgctgc  >  2:2811853/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCTGGACTGGCAGCCCGCCAGCAACCAGTTGCTGTGGGTCGCCAGCACCGGCCTGCCGGCAGCCGAAGGTGGCTCGCGCCTGCAACGCCAGCGCCTGCGGTTCGAGGCCCGCGAGCAACGCCTGCTGCTGGAAAGCTCGGCGGACCTGTACGCCGCTGGCGCCCCGCGCTGGGTACAGCGCGAACGACTCGACCAGGTCAGCGCCATGAACGTTCTGTTCTACCAGGGCGGCCGCTGGCTGGCCTGGCCTTCTGACCAACCGGTGCACCCAGGTCGTGGTGTGCGTCTGGAACTGCTGC  >  NC_002947/1206791‑1207089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑