BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,261,837	G→T	5.8%	noncoding (433/451 nt)	PP_mr14 ←	cspA regulatory region

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,261,837	0	G	T	5.8%	2951.9 / 113.8	872	noncoding (433/451 nt)	PP_mr14	cspA regulatory region
Reads supporting (aligned to +/- strand): ref base G (398/422); new base T (25/26); total (424/448)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 876 total aligned reads displayed.

TTACCATCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATGGCTTT  >  NC_002947/1261689‑1261986

 tACCATCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGaa                                                                                                                                                     <  1:1824847/150‑1 (MQ=255)

      tCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGTGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCgg                                                                                                                                                >  2:8612702/1‑150 (MQ=255)

      tCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCgg                                                                                                                                                >  2:4073022/1‑150 (MQ=255)

         gCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGtt                                                                                                                                             >  2:6156706/1‑150 (MQ=255)

          cGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCTTCTGGCGGGttt                                                                                                                                            <  1:383103/150‑1 (MQ=255)

            aaGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCTTCTGGCGGGttttt                                                                                                                                          <  2:3480157/150‑1 (MQ=255)

            aaGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGttttt                                                                                                                                          <  2:1417379/150‑1 (MQ=255)

              gACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCTTCTGGCGGGttttttt                                                                                                                                        <  1:7892943/150‑1 (MQ=255)

              gACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGttttttt                                                                                                                                        >  1:3991520/1‑150 (MQ=255)

              gACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGttttttt                                                                                                                                        <  2:3337123/150‑1 (MQ=255)

              gACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACGGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGttttttt                                                                                                                                        <  1:5288819/150‑1 (MQ=255)

              gACGGACCTGCCATACCGAGATTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGttttttt                                                                                                                                        <  2:4540989/150‑1 (MQ=255)

                cTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGa                                                                                                                                      >  2:7909903/1‑150 (MQ=255)

                 ggACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGaa                                                                                                                                     <  2:8381330/149‑1 (MQ=255)

                     cTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCt                                                                                                                                 <  1:8079093/150‑1 (MQ=255)

                      cgCCATACCGAGATTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGATTTTTTGAAAGCTa                                                                                                                                <  1:2314286/149‑1 (MQ=255)

                       gCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAg                                                                                                                               <  2:5091427/150‑1 (MQ=255)

                            aCCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGc                                                                                                                          >  2:7629108/1‑150 (MQ=255)

                            aCCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGc                                                                                                                          >  2:148984/1‑150 (MQ=255)

                             ccGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCt                                                                                                                         >  2:3717637/1‑150 (MQ=255)

                              cGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTa                                                                                                                        >  1:8472779/1‑150 (MQ=255)

                                aGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGa                                                                                                                      <  2:4145852/150‑1 (MQ=255)

                                aGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGa                                                                                                                      <  1:1007143/150‑1 (MQ=255)

                                    tGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGa                                                                                                                  >  2:2814190/1‑150 (MQ=255)

                                     gCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGAc                                                                                                                 >  2:4264442/1‑150 (MQ=255)

                                     gCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGAc                                                                                                                 <  2:1711928/150‑1 (MQ=255)

                                       ccGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACtt                                                                                                               >  2:4462717/1‑150 (MQ=255)

                                        cGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCGCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTa                                                                                                              <  2:6679025/150‑1 (MQ=255)

                                        cGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTa                                                                                                              >  2:6376641/1‑150 (MQ=255)

                                        cGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTa                                                                                                              <  1:908105/150‑1 (MQ=255)

                                             acaGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGa                                                                                                         >  2:5282503/1‑150 (MQ=255)

                                             acaGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGa                                                                                                         >  2:899288/1‑150 (MQ=255)

                                               aGCGACTGCGCCGTCCTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAATCTAGATCGACTTAGGCGATc                                                                                                       <  1:6862443/150‑1 (MQ=255)

                                               aGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATc                                                                                                       <  1:6371745/150‑1 (MQ=255)

                                               aGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATc                                                                                                       >  1:5130493/1‑150 (MQ=255)

                                                 cGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGCCTAAGTCGATCta                                                                                                     <  1:3149348/150‑2 (MQ=255)

                                                    cTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGATTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAAc                                                                                                  <  2:2032068/150‑1 (MQ=255)

                                                       cgcCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAActtc                                                                                               <  2:2268433/150‑1 (MQ=255)

                                                       cgcCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAActtc                                                                                               <  1:464517/150‑1 (MQ=255)

                                                          cGTACTTGAAGCCGGTGCAGTCCCGGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACttcttc                                                                                            <  1:4392186/150‑1 (MQ=255)

                                                               ttGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCGGCCt                                                                                       >  2:6268551/1‑150 (MQ=255)

                                                               ttGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCt                                                                                       >  2:2624017/1‑150 (MQ=255)

                                                                 gAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGc                                                                                     >  1:1473251/1‑150 (MQ=255)

                                                                  aaGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCa                                                                                    >  1:3019129/1‑150 (MQ=255)

                                                                   aGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCAt                                                                                   <  1:4312079/150‑1 (MQ=255)

                                                                     ccTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGc                                                                                 >  2:4721558/1‑150 (MQ=255)

                                                                          gCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGAc                                                                                                                 >  1:1936117/1‑113 (MQ=255)

                                                                           cAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCt                                                                           <  1:6785920/150‑1 (MQ=255)

                                                                            aGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTg                                                                          <  1:2830555/150‑1 (MQ=255)

                                                                            aGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTg                                                                          <  1:8604183/150‑1 (MQ=255)

                                                                             gTCCCTGTGGGCGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTgg                                                                         <  2:8481933/150‑1 (MQ=255)

                                                                               cccTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAAc                                                                                                  <  2:1857769/123‑1 (MQ=255)

                                                                                 cTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCcgc                                                                     >  2:8006822/1‑150 (MQ=255)

                                                                                  tgtgGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCcgcg                                                                    >  1:800302/1‑150 (MQ=255)

                                                                                  tgtgGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCcgcg                                                                    >  2:8147462/1‑150 (MQ=255)

                                                                                   gtgGGAGCGTGCTAGCCCGCGAAGAGGACAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGATAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCgg                                                                   <  2:5512278/150‑1 (MQ=255)

                                                                                   gtgGGAGCGGGCGAGCCCGCGCAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCgg                                                                   <  2:3119324/150‑1 (MQ=255)

                                                                                       tcgCGGGCTCGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAg                                                               >  1:8171509/3‑150 (MQ=255)

                                                                                       gAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAg                                                               >  1:83362/1‑150 (MQ=255)

                                                                                               gAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCATCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGaaa                                                       <  1:1265923/150‑1 (MQ=255)

                                                                                                   ccGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTaa                                                   >  2:2963571/1‑150 (MQ=255)

                                                                                                     gcgAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACa                                                 <  2:8239575/150‑1 (MQ=255)

                                                                                                                   gcCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCttt                                   <  2:3927377/150‑1 (MQ=255)

                                                                                                                   gcCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCttt                                   <  1:6516761/150‑1 (MQ=255)

                                                                                                                     cTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCa                                 <  1:4498896/150‑1 (MQ=255)

                                                                                                                        tttCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAggg                              >  1:6022238/1‑150 (MQ=255)

                                                                                                                        tttCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAggg                              >  1:3240201/1‑150 (MQ=255)

                                                                                                                        tttCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTACATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCGGGCCTTCTTTCAggg                              >  1:6270417/1‑150 (MQ=255)

                                                                                                                           cGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGttt                           >  1:8712734/1‑150 (MQ=255)

                                                                                                                           cGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGttt                           <  1:7441426/150‑1 (MQ=255)

                                                                                                                           cGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGttt                           <  1:3442070/150‑1 (MQ=255)

                                                                                                                            gAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGtttt                          <  2:4894039/150‑1 (MQ=255)

                                                                                                                             aGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTg                         <  1:2504841/150‑1 (MQ=255)

                                                                                                                              gCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGa                        <  1:4177330/150‑1 (MQ=255)

                                                                                                                               cAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGaa                       <  1:4943166/150‑1 (MQ=255)

                                                                                                                               cAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGaa                       <  2:5840128/150‑1 (MQ=255)

                                                                                                                                aGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAg                      >  2:1192311/1‑150 (MQ=255)

                                                                                                                                  gCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGcc                    <  1:2642456/150‑1 (MQ=255)

                                                                                                                                  gCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGcc                    <  2:1621205/150‑1 (MQ=255)

                                                                                                                                  gCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGcc                    >  1:5496263/1‑150 (MQ=255)

                                                                                                                                    aTAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGt                  >  1:5692466/1‑150 (MQ=255)

                                                                                                                                    aTAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGt                  >  1:6132254/1‑150 (MQ=255)

                                                                                                                                    aTAAAAAAACCCGACAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGAATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGt                  >  1:6623655/1‑150 (MQ=255)

                                                                                                                                      aaaaaaaCCCGCCTTCTGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCa                >  1:7729916/1‑150 (MQ=255)

                                                                                                                                       aaaaaaCCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAg               <  2:2373770/150‑1 (MQ=255)

                                                                                                                                       aaaaaaCCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAg               <  1:7629108/150‑1 (MQ=255)

                                                                                                                                       aaaaaaCCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAg               >  2:7407311/1‑150 (MQ=255)

                                                                                                                                        aaaaaCCCGCCTTCTGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGc              >  1:7040309/1‑150 (MQ=255)

                                                                                                                                           aaCCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTTGCCTTCTTTCAGGGTTGTGAAGCCGTCAGCTTg           <  1:3717637/150‑1 (MQ=255)

                                                                                                                                           aaCCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTg           >  1:2134907/1‑150 (MQ=255)

                                                                                                                                              ccGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGAt        >  2:6929591/1‑150 (MQ=255)

                                                                                                                                              ccGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGAt        >  2:5637477/1‑150 (MQ=255)

                                                                                                                                              ccGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGAt        >  1:7206759/1‑150 (MQ=255)

                                                                                                                                              ccGCCAGAAGGCGGGATTTATTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGAt        <  2:6469924/150‑1 (MQ=255)

                                                                                                                                               cGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATg       >  1:5856569/1‑150 (MQ=255)

                                                                                                                                               cGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATg       >  2:6584211/1‑150 (MQ=255)

                                                                                                                                                  cAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATGGCt    <  1:4813793/150‑1 (MQ=255)

                                                                                                                                                   aGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATGGCtt   >  2:8703471/1‑150 (MQ=255)

                                                                                                                                                   aGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATGGCtt   >  1:1645651/1‑150 (MQ=255)

                                                                                                                                                    gAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATGGCttt  >  2:7128420/1‑150 (MQ=255)

TTACCATCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATGGCTTT  >  NC_002947/1261689‑1261986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`