BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,315,402	G→C	5.0%	H362Q (CAC→CAG)	PP_1144 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,315,402	0	G	C	5.0%	2319.3 / 74.7	678	H362Q (CAC→CAG)	PP_1144	membrane protein
Reads supporting (aligned to +/- strand): ref base G (294/350); new base C (15/19); total (309/369)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 682 total aligned reads displayed.

TGGTGGTCATGACCATGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAGGCGGTTTTCGCTGGCCTGATGTTCGAC  >  NC_002947/1315254‑1315551

 ggtggtCATGACCATGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTg                                                                                                                                                     <  2:2439016/150‑1 (MQ=255)

 ggtggtCATGACCATGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTg                                                                                                                                                     <  1:3793790/150‑1 (MQ=255)

   tggtCATGACCATGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGTCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGt                                                                                                                                                   >  2:7770759/1‑150 (MQ=255)

   tggtCATGACCATGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGt                                                                                                                                                   >  1:2915659/1‑150 (MQ=255)

    ggtCATGACCATGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGtt                                                                                                                                                  <  2:8437773/150‑1 (MQ=255)

      tCATGACCATGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGc                                                                                                                                                <  1:2032935/150‑1 (MQ=255)

        aTGACCATGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCgg                                                                                                                                              <  1:325166/150‑1 (MQ=255)

        aTGACCATGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCgg                                                                                                                                              <  2:2904833/150‑1 (MQ=255)

        aTGACCATGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCgg                                                                                                                                              <  2:3237392/150‑1 (MQ=255)

        aTGACCATGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCgg                                                                                                                                              <  2:4605333/150‑1 (MQ=255)

        aTGACCATGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCgg                                                                                                                                              >  1:6161512/1‑150 (MQ=255)

        aTGACCATGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCgg                                                                                                                                              <  2:8684041/150‑1 (MQ=255)

         tGACCATGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCggg                                                                                                                                             <  1:1883035/150‑1 (MQ=255)

         ggACCATGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCggg                                                                                                                                             <  2:3057237/149‑1 (MQ=255)

           aCCATGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTa                                                                                                                                           >  2:6170734/1‑150 (MQ=255)

           aCCATGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCCCGCAACCACGACCTGGTTGCgcgct                                                                                                                                           <  1:4316603/150‑5 (MQ=255)

             cATGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGc                                                                                                                                         >  1:3353504/1‑150 (MQ=255)

             cATGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGc                                                                                                                                         >  2:1268887/1‑150 (MQ=255)

             cATGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGc                                                                                                                                         >  1:6675404/1‑150 (MQ=255)

             cATGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCCCGCAACCACGACCTGGTTGCgcgcttt                                                                                                                                         <  2:1597565/150‑7 (MQ=255)

              aTGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCCCGCAACCACGACCTGGTTGCgcgctttg                                                                                                                                        <  2:8767991/150‑8 (MQ=255)

               tGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCgg                                                                                                                                       >  2:8226636/1‑150 (MQ=255)

                gcgcCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGc                                                                                                                                      <  1:8083158/150‑1 (MQ=255)

                gcgcCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGc                                                                                                                                      <  1:7286460/150‑1 (MQ=255)

                    cgcccgccCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTg                                                                                                                                  >  2:5467868/1‑150 (MQ=255)

                    cgcccgccCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTg                                                                                                                                  >  2:5641372/1‑150 (MQ=255)

                     gcccgcccAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTCGCGGCCTTGa                                                                                                                                 >  2:6669989/1‑150 (MQ=255)

                     gcccgcccAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGa                                                                                                                                 <  2:2703262/150‑1 (MQ=255)

                      cccgcccAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGAt                                                                                                                                <  2:7656020/150‑1 (MQ=255)

                      cccgcccAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGAt                                                                                                                                <  1:1710965/150‑1 (MQ=255)

                       ccgcccAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGAtg                                                                                                                               <  1:958171/150‑1 (MQ=255)

                         gcccAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGAtgtg                                                                                                                             >  2:381104/1‑150 (MQ=255)

                          cccAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTgg                                                                                                                            <  1:7098857/150‑1 (MQ=255)

                          cccAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCATGATGTgg                                                                                                                            <  1:1262392/150‑1 (MQ=255)

                           ccAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGt                                                                                                                           >  2:5518307/1‑150 (MQ=255)

                                        gTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCtt                                                                                                              >  2:3901017/1‑150 (MQ=255)

                                        gTCTTTCATCCGTTGCATGATGCGCAGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTCACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCtt                                                                                                              >  1:3087166/1‑150 (MQ=255)

                                           tttCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGag                                                                                                           <  2:6060432/150‑1 (MQ=255)

                                           tttCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGag                                                                                                           >  1:5933470/1‑150 (MQ=255)

                                            ttCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGa                                                                                                            >  1:3550345/1‑148 (MQ=255)

                                               aTCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGagcagc                                                                                                       <  2:4464069/150‑1 (MQ=255)

                                                 ccGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTc                                                                                                     <  2:2224900/150‑1 (MQ=255)

                                                  cGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCg                                                                                                    >  1:5015638/1‑150 (MQ=255)

                                                               gcTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGcgtcgt                                                                                       <  2:5899136/150‑1 (MQ=255)

                                                                cTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTg                                                                                      <  2:7986559/150‑1 (MQ=255)

                                                                   ggCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGcc                                                                                   >  2:5123330/1‑150 (MQ=255)

                                                                     ccAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACCGACCTCGTCGCCACGAAAGCGCGCACCCAGTTCGTGGTTGAGGGTAGCGGCGTTGAGGTGGTTGTCGATCACCTTGAGCAGCTCGTCAGCAGCGTGGTGGCCaa                                                                                 <  2:371780/150‑1 (MQ=255)

                                                                     ccAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCaa                                                                                 >  2:6589329/1‑150 (MQ=255)

                                                                      cAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTCGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAg                                                                                >  2:5321596/1‑150 (MQ=255)

                                                                       aGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAgg                                                                               >  1:7207263/1‑150 (MQ=255)

                                                                              gggCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGt                                                                        >  1:8511397/1‑150 (MQ=255)

                                                                                  ttCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGAt                                                                    >  2:1136576/1‑150 (MQ=255)

                                                                                    cgtcgCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATAc                                                                  <  2:8144592/150‑1 (MQ=255)

                                                                                     gtcgCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGAGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGAGGCCAAGGCTGTCGTTGATACg                                                                 >  1:1000366/1‑150 (MQ=255)

                                                                                      tcgCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGc                                                                >  1:6317252/1‑150 (MQ=255)

                                                                                      tcgCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGc                                                                <  1:4969719/150‑1 (MQ=255)

                                                                                           gCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGaa                                                           <  2:1402860/150‑1 (MQ=255)

                                                                                             tGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAAt                                                         >  2:2837839/1‑150 (MQ=255)

                                                                                                tCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGt                                                      <  1:8273841/150‑1 (MQ=255)

                                                                                                  aGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCg                                                    >  1:3843312/1‑150 (MQ=255)

                                                                                                  aGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCg                                                    <  1:7366879/150‑1 (MQ=255)

                                                                                                  aGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCg                                                    <  2:5974177/150‑1 (MQ=255)

                                                                                                   ggCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGa                                                   >  2:7334765/1‑150 (MQ=255)

                                                                                                    gCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGAt                                                  <  2:6844037/150‑1 (MQ=255)

                                                                                                       ggTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTc                                               >  1:2828190/1‑150 (MQ=255)

                                                                                                         tGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGa                                             <  1:4740722/150‑1 (MQ=255)

                                                                                                         tGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGa                                             <  1:5676192/150‑1 (MQ=255)

                                                                                                         tGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGa                                             >  2:1992753/1‑150 (MQ=255)

                                                                                                          gACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAg                                            <  2:3129432/150‑1 (MQ=255)

                                                                                                           aCCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAgg                                           >  2:7077774/1‑150 (MQ=255)

                                                                                                             cacacaGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGaa                                         >  2:4957955/1‑150 (MQ=255)

                                                                                                              acacaGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAAc                                        >  1:7988483/1‑150 (MQ=255)

                                                                                                                acaGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACAt                                      <  2:6484404/150‑1 (MQ=255)

                                                                                                                acaGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACAt                                      >  1:6926542/1‑150 (MQ=255)

                                                                                                                  aGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGa                                    >  2:3354959/1‑150 (MQ=255)

                                                                                                                    aaCTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACa                                  <  1:8422257/150‑1 (MQ=255)

                                                                                                                        cgtcgCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCa                              <  1:7806284/150‑1 (MQ=255)

                                                                                                                          tcgCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAgg                            <  2:7200386/150‑1 (MQ=255)

                                                                                                                            gCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAGGCg                          >  1:4042001/1‑150 (MQ=255)

                                                                                                                             ccaccaAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAGGCgg                         >  2:4070917/1‑150 (MQ=255)

                                                                                                                               accaAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAGGCGGtt                       >  1:8752169/1‑150 (MQ=255)

                                                                                                                                 caAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAGGCGGtttt                     <  2:4221617/150‑1 (MQ=255)

                                                                                                                                  aaaGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAGGCGGTTTTc                    <  1:681935/150‑1 (MQ=255)

                                                                                                                                  aaaGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAGGCGGTTTTc                    <  1:3636347/150‑1 (MQ=255)

                                                                                                                                  aaaGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAGGCGGTTTTc                    <  1:5468183/150‑1 (MQ=255)

                                                                                                                                    aGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCACGTCACCGGCGTCGTGGTCAAGGCTGTCGTTGATACGCTGGAAAGGGTCGATGTCGAGGAACATGACAGCCAGGCGGTTTTCGc                  <  2:1915661/150‑1 (MQ=255)

                                                                                                                                     gcgcgcAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAGGCGGTTTTCGCt                 >  1:5236375/1‑150 (MQ=255)

                                                                                                                                     gcgcgcAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAGGCGGTTTTCGCt                 >  2:2564534/1‑150 (MQ=255)

                                                                                                                                      cgcgcAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAGGCGGTTTTCGCTg                <  2:7504344/150‑1 (MQ=255)

                                                                                                                                       gcgcAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAGGCGGTTTTCGCTgg               >  1:7570665/1‑150 (MQ=255)

                                                                                                                                       gcgcAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAGGCGGTTTTCGCTgg               >  1:2373672/1‑150 (MQ=255)

                                                                                                                                        cgcAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAGGCGGTTTTCGCTGGc              <  1:2540783/150‑1 (MQ=255)

                                                                                                                                        cgcAACCACGACCTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAGGCGGTTTTCGCTGGc              >  1:6609230/1‑150 (MQ=255)

                                                                                                                                          cAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAGGCGGTTTTCGCTGGCCt            <  1:4215099/150‑1 (MQ=255)

                                                                                                                                           aaCCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAGGCGGTTTTCGCTGGCCTg           <  2:6523216/150‑1 (MQ=255)

                                                                                                                                             ccAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAGGCGGTTTTCGCTGGCCTGAt         >  2:5755862/1‑150 (MQ=255)

                                                                                                                                             ccAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAGGCGGTTTTCGCTGGCCTGAt         <  2:3603743/150‑1 (MQ=255)

                                                                                                                                                 gTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAGGCGGTTTTCGCTGGCCTGATGTTc     <  1:2137404/150‑1 (MQ=255)

                                                                                                                                                   cGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAGGCGGTTTTCGCTGGCCTGATGTTCGa   <  1:5840861/150‑1 (MQ=255)

                                                                                                                                                    gTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAGGCGGTTTTCGCTGGCCTGATGTTCGAc  <  1:150547/150‑1 (MQ=255)

TGGTGGTCATGACCATGCGCCGCCCGCCCAGGTCGATGGGGTCTTTCATCCGTTGCATGATGCGCTGGGCCAGGTGGCGGGCTTCGTCGCGGCTGTTCAGGCTGGTGACCACACAGAACTCGTCGCCACCAAAGCGCGCAACCAGGTCGTGGTTGCGGGTAGCGGCCTTGATGTGGTTGGCGATCACCTTGAGCAGCTCGTCACCGGCGTCGTGGCCAAGGCTGTCGTTGATACGCTTGAAATGGTCGATGTCGAGGAACATGACAGCCAGGCGGTTTTCGCTGGCCTGATGTTCGAC  >  NC_002947/1315254‑1315551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`