Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 170,839 T→G 5.1% intergenic (+20/‑550) PP_0159 → / → PP_16SA family III CoA‑transferase/16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947170,8390TG 5.1% 732.7 / 16.3 259intergenic (+20/‑550)PP_0159/PP_16SAfamily III CoA‑transferase/16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (120/117);  new base G (7/9);  total (128/131)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 262 total aligned reads displayed.

CGGAGACGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAGGCA  >  NC_002947/170692‑170988
                                                                                                                                                   |                                                                                                                                                     
cGGAGACGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCa                                                                                                                                                     <  2:426383/150‑1 (MQ=255)
cGGAGACGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCa                                                                                                                                                     <  2:5338116/150‑1 (MQ=255)
cGGAGACGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCa                                                                                                                                                     <  2:6885710/150‑1 (MQ=255)
 ggAGACGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAg                                                                                                                                                    <  1:7712054/150‑1 (MQ=255)
       gCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCtac                                                                                                                                              >  1:5872666/1‑148 (MQ=255)
       gCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTcc                                                                                                                                              >  1:1908136/1‑150 (MQ=255)
        ccAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGACGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCTGACGGCCTccc                                                                                                                                             <  2:1493388/150‑1 (MQ=255)
        ccAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCTGACGGCCTccc                                                                                                                                             <  1:2946165/150‑1 (MQ=255)
         cAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCTGACGGCCTcccc                                                                                                                                            <  2:6605538/150‑1 (MQ=255)
         cAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTcccc                                                                                                                                            <  1:4103688/150‑1 (MQ=255)
         cAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCGcccc                                                                                                                                            >  1:3890905/1‑150 (MQ=255)
           gTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTcccccc                                                                                                                                          >  1:5230320/1‑148 (MQ=255)
              gAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGACGTGTTGGGGCTGGATGCTGGCGAGGTCCAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGGCAGGCCGCCCCGcggt                                                                                                                                       >  1:2336886/1‑147 (MQ=255)
                 tATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCCCTTTGtt                                                                                                                                    >  2:1034634/1‑150 (MQ=255)
                 tATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCGCCCCCCtttttt                                                                                                                                    >  1:1662585/1‑150 (MQ=255)
                 tATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCACCCCGCgttgtt                                                                                                                                    >  1:7594727/1‑150 (MQ=255)
                   tCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGtttt                                                                                                                                  >  1:2041103/1‑150 (MQ=255)
                   tCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGtttt                                                                                                                                  >  2:1518145/1‑150 (MQ=255)
                    cGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGGGGTGCTGGGGGAAGTTTTGGGGCTGGATGCTGGCGAGGTACCGCGTCTGCGTGATGCTGGGGTGCTCTATATGAAATGCGGGGAGGCCTTAAGGCCTCCCCGCTTTGttttt                                                                                                                                 <  2:2272258/150‑1 (MQ=255)
                     gCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGTGGGGAGGCCTGACGGCCTCCCCGCttttcatcta                                                                                                                                <  2:2545916/150‑8 (MQ=39)
                         ggCGCCACCAGTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGTTGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATTAAAAGGGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAgcg                                                                                                                            <  1:8634831/150‑1 (MQ=255)
                         ggCGCCACCACTGCTGGGCGAGCATACCGATGTGGTGCTGGGGTAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGAGGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAgcg                                                                                                                            <  1:7143204/150‑1 (MQ=255)
                          gcgcCACCACTGCTGGGCGAGCATCCCGACGTGGTGCTGGGGGACGTGTGGGGGCTGGATGCTGGCGCGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAgcgc                                                                                                                           <  2:7461090/150‑1 (MQ=39)
                            gcCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCGAGATGAAAAGCGGGGAGGCCGGCAGGCCGCCCCGCGTTGTTTTTCAgcgcga                                                                                                                         >  1:1274183/1‑148 (MQ=255)
                              caccacTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTAAGAGCGAta                                                                                                                       >  1:6212871/1‑149 (MQ=255)
                               accacTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCa                                                                                                                      >  2:4607375/1‑150 (MQ=255)
                               accacTGCTGGGCGAGCATACCCAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGCGGCCGTCAGGCCTCCCCGCGTTGTTTTTCAGCGCGATCa                                                                                                                      >  2:7404974/1‑150 (MQ=255)
                                 ccctggtgGGCGAGCATACGGATGTGGTGGTGGGGGAAGTGTTGGGGCTGGTTGCTGGGGCGGTACCGGGTCTGCGTGTTGCTGGGGTGCTCTAGATGAAACGCGGGGCGGCGGTCCGGCCTCCCCGCTTTGTTTTTCAGCGCTATCaaa                                                                                                                    <  1:2651155/148‑1 (MQ=255)
                                 cacTGCTGGGCGAGCATACCGACGGGGTGCTGGGGGAAGTGTTGGGGCTGGTTGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCaaa                                                                                                                    <  2:4005390/150‑1 (MQ=255)
                                 cacTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCaaa                                                                                                                    >  1:668375/1‑150 (MQ=255)
                                  acTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACCGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAg                                                                                                                   <  2:8134435/150‑1 (MQ=255)
                                  acTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCTTCTGCGTGATGCTGGGGTGCTCTATATGAAAAGCGGGGTGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAg                                                                                                                   <  2:1168669/150‑1 (MQ=255)
                                  acTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAg                                                                                                                   >  2:8013676/1‑150 (MQ=255)
                                    tgctgGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTTTTTTTAAGCGCGATAAAAGGt                                                                                                                 >  1:8225308/1‑150 (MQ=255)
                                        gggCGAGCATACCGATGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAAtt                                                                                                             <  1:6468737/150‑1 (MQ=255)
                                         ggCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCGCCCCCCGTTGTTTTTAAGCGCTATCAAAGGTaaatt                                                                                                            >  1:3653999/1‑147 (MQ=255)
                                          gCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGa                                                                                                           >  2:2131741/1‑150 (MQ=255)
                                            gAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGaaa                                                                                                         <  2:3599788/150‑1 (MQ=255)
                                                  aCCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGTTGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAg                                                                                                   <  2:2827425/150‑1 (MQ=255)
                                                  aCCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGCGGCCGGCAGGGCACCCCGCGTTGTTTTTCAGCGCTAAAAAAGGTAATTTAAAATAAAg                                                                                                   >  1:4656068/1‑150 (MQ=255)
                                                    cGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGt                                                                                                 >  1:3548716/1‑150 (MQ=255)
                                                    cGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCAATAAAATGTAAATGAAAATAAAGGt                                                                                                 >  2:2596086/1‑150 (MQ=39)
                                                      taGTGGTGCTGGGGGATGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGCGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTg                                                                                               <  2:3260509/149‑1 (MQ=255)
                                                              cTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGGCAGGCCGCCCCCCTTTGTTTTTCAGCGCGATCAAAGGTAAATGAAAATAAATGTTGAAGcccac                                                                                       >  1:3282682/1‑148 (MQ=255)
                                                                gggggATGTGTTGGGGCTGTTTGCTGGCGAGGTACAGCGTCTGCGTGTTGCTGGGGTGCTCTATATGAAAAGCGGGGAGGCCGTCAGGCCACCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCtctc                                                                                     <  1:8589911/150‑1 (MQ=255)
                                                                gggggAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCtctc                                                                                     >  2:2811742/1‑150 (MQ=255)
                                                                 ggggAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCg                                                                                    >  2:5329690/1‑150 (MQ=255)
                                                                    gAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGACAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCACTCGaaa                                                                                 >  2:1858815/1‑149 (MQ=255)
                                                                      aGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGGGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATcc                                                                               <  1:5764050/150‑1 (MQ=255)
                                                                        tgtTGGGGCTGTTTGCGGGCGTGGTACCGGGTCTGGGTGATGCTGGGGTGCTCTAGATGAAAAGGGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATcccc                                                                             <  1:5564868/150‑1 (MQ=255)
                                                                          ttGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCACCCCCCTTTGTTTTTAAGCGCTAAAAAAAGTAATTGAAATTAAAGGTTGACGCCCCCACGAATacccgt                                                                           >  1:915489/1‑148 (MQ=255)
                                                                           tGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTta                                                                          <  1:722034/150‑1 (MQ=255)
                                                                            ggggCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTtat                                                                         >  2:7674812/1‑150 (MQ=255)
                                                                             gggCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTtata                                                                        >  2:4481416/1‑150 (MQ=255)
                                                                             gggCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCACCCCGCGTTGTTTTTGAGCGCTATCAAAGGTAATTGAAAATAAATGTTGAAGCCCTCTAGAATACCCTtata                                                                        >  1:4819515/1‑150 (MQ=255)
                                                                                         ggCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGGGGCCGTCAGGCCGCCCCCCTTTGTTTTTGAGCGCTATCAAATGTAATTTAAAATTAATGTTTACGCCCGCTCGAATACCCGAATAAATCGCCCCAca                                                            >  1:8390438/1‑149 (MQ=255)
                                                                                          gagAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAAATAAAGGTTGTCGCCCTCTCGAATACCCTTATAATGCGCCCCACtt                                                           >  2:6582010/3‑150 (MQ=255)
                                                                                           ctcggTCCAGCGTCTGCGTGTTGCTGGGGTGCTCTAGATTAAAAGCGGGGCGGCCGCCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTc                                                          <  1:431692/147‑1 (MQ=37)
                                                                                            gAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGGCGGCAGGCCTCCCCGCGTTGTTTTTCAGCGCTATAAAAGGTAAATTAAAATAAAGGTTGAAGCCCTCACGAAAACCCGTATAATGCGCCCCACATcc                                                         >  1:2271565/1‑150 (MQ=255)
                                                                                             aGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCTCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCa                                                        <  1:1012248/150‑1 (MQ=255)
                                                                                               gTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAACGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGc                                                      <  2:3566054/150‑1 (MQ=255)
                                                                                               gTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGc                                                      <  2:5064820/150‑1 (MQ=255)
                                                                                                   aGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACa                                                  >  2:6325889/1‑150 (MQ=255)
                                                                                                    gCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACAt                                                 <  2:5451559/150‑1 (MQ=255)
                                                                                                      gTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCCGGCCGCCCCGCGTTGTTTTTAAGCGCGATCAAAGGTAATTGAAATTAAAGGTTGACGCCCACGCGCAACCCCGTATAATGCGCCCCCCGTCCCGCGACAGCg                                               >  1:505533/1‑150 (MQ=37)
                                                                                                      gTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGGTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTTTAATGCGCCCCACTTCCAGCGACATCg                                               >  1:635050/1‑150 (MQ=255)
                                                                                                        cTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGa                                             >  2:6060895/1‑150 (MQ=255)
                                                                                                        cTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGa                                             >  2:6457606/1‑150 (MQ=255)
                                                                                                        cTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGa                                             >  2:5730625/1‑150 (MQ=255)
                                                                                                        cTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCATATAATGCGCCCCACTTCCAGCGACATCGGa                                             >  2:1309169/1‑150 (MQ=255)
                                                                                                         tGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGaa                                            <  2:2019684/150‑1 (MQ=255)
                                                                                                               ctGCTGGGGTGCTCTAGATGAAAAGCGGGGCGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGaaaa                                      <  2:3653999/149‑1 (MQ=255)
                                                                                                                tGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGaaaaa                                     <  2:8636188/150‑1 (MQ=255)
                                                                                                                tGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGaaaaa                                     <  2:5180114/150‑1 (MQ=255)
                                                                                                                 gCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATTCGCCCCACATCCAGCGACATCGGAACGAAAAAc                                    >  2:6216832/1‑150 (MQ=255)
                                                                                                                 gCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCCCTTTGTTTTTAAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACATCCAGCGACATAGGAAAGAAAAAc                                    >  1:7272341/1‑150 (MQ=37)
                                                                                                                   tGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTTACGCCCTCTCGAATCCCCTTATAATTCGCCCCACTTCCAGCGACATAGGAACGAAAAACTc                                  >  2:6832095/1‑150 (MQ=255)
                                                                                                                       gTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATC‑AAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTga                             <  2:485866/150‑1 (MQ=255)
                                                                                                                           tctAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTTTTTTTCAGCGCTATAAAAGGTAATTGAAATTAAAGGTTGACGCCCACTCGAATACCCATATAATTCGCCCCACATCCAGCGACAACGGAACGAAAAACTACTTTAGAt                          >  1:4953781/1‑150 (MQ=25)
                                                                                                                             tAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACAACGGAACGAAAAACTCCTTGAGATTc                        >  2:3491361/1‑150 (MQ=255)
                                                                                                                              aGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGATGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCa                       <  2:7530650/150‑1 (MQ=255)
                                                                                                                               taTGAAATGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCaa                      <  1:998558/149‑1 (MQ=255)
                                                                                                                               gATGAAACGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCaa                      <  2:7706566/150‑1 (MQ=255)
                                                                                                                               gATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCaa                      <  2:914614/150‑1 (MQ=255)
                                                                                                                               gATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCaa                      <  2:5230320/150‑1 (MQ=255)
                                                                                                                                aTGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAAt                     <  2:1666637/150‑1 (MQ=255)
                                                                                                                                 tGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATg                    >  2:4159623/1‑150 (MQ=255)
                                                                                                                                     aaGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGtt                >  1:3026902/1‑150 (MQ=255)
                                                                                                                                       gCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCGTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAAGGAGTTaa              >  1:5380017/1‑150 (MQ=255)
                                                                                                                                         ggggAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAagt            >  1:4568071/1‑150 (MQ=255)
                                                                                                                                           ggAGGCCGTCAGGCCTCCCCTCTTTTATTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCACTCTCGAATCCCCTTATAATGCGCCACACTTCAAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAagtag          <  2:5872666/150‑1 (MQ=18)
                                                                                                                                             aGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGtt        <  2:1908136/150‑1 (MQ=255)
                                                                                                                                               gCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTc                                                          >  2:7178221/1‑98 (MQ=255)
                                                                                                                                               gCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTaa      <  2:3913725/150‑1 (MQ=255)
                                                                                                                                               gCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTaa      >  1:6202533/1‑150 (MQ=255)
                                                                                                                                                cggTCTGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATTATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAg     <  1:5421111/148‑1 (MQ=255)
                                                                                                                                                ccGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAg     >  2:3444688/1‑150 (MQ=255)
                                                                                                                                                 cGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCTATGAGTTAAGTAGTTAAgg    <  2:7937533/150‑1 (MQ=255)
                                                                                                                                                  gTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAGGc   >  1:2699324/1‑150 (MQ=255)
                                                                                                                                                   tCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAGGCa  >  2:8550591/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CGGAGACGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAGGCA  >  NC_002947/170692‑170988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑