BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,385,677	G→T	5.8%	intergenic (‑110/+25)	PP_t67 ← / ← PP_t66	tRNA‑Met/tRNA‑Met

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,385,677	0	G	T	5.8%	1139.5 / 43.0	354	intergenic (‑110/+25)	PP_t67/PP_t66	tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand): ref base G (180/153); new base T (4/17); total (184/170)
Fisher's exact test for biased strand distribution p-value = 2.59e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.79e-01

Only 100 of 364 total aligned reads displayed.

TTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTAG  >  NC_002947/1385529‑1385825

ttATGAGCCAGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTATATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTgtgt                                                                                                                                                     <  1:4307517/150‑1 (MQ=255)

   tGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCTGTGGGGCGGATTCTACATTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTaa                                                                                                                                                  <  2:6367256/150‑1 (MQ=255)

     aGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACa                                                                                                                                                <  1:4670118/150‑1 (MQ=255)

       cccGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGt                                                                                                                                              <  2:1053573/150‑1 (MQ=255)

        ccGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTg                                                                                                                                             <  2:7519987/150‑1 (MQ=255)

         cgacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTgg                                                                                                                                            <  2:1942030/150‑1 (MQ=255)

          gacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGc                                                                                                                                           <  2:5923901/150‑1 (MQ=255)

             gaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCtt                                                                                                                                        <  2:3261275/150‑1 (MQ=255)

               gCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAGGGCCACTGTTAACACAGTGGCCtttt                                                                                                                                      <  1:458468/150‑1 (MQ=18)

               gCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCtttt                                                                                                                                      <  1:2366062/150‑1 (MQ=255)

               gCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCtttt                                                                                                                                      <  1:3021554/150‑1 (MQ=255)

                cTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTc                                                                                                                                     <  1:2049422/150‑1 (MQ=255)

                cTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTGTTCGCGAATGGTTATAGGCAGGCAATGAAATAGGCAACTGTGTTACGAGTGGCCTTTTc                                                                                                                                     >  2:792972/1‑150 (MQ=18)

                 tACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTCAACAGTGGCCTTTTCt                                                                                                                                    <  1:8799118/150‑1 (MQ=255)

                   ccAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAG‑AAAAGGCCACTGTTAACACAGTGGCCtttttcttt                                                                                                                                 <  2:6903847/150‑6 (MQ=18)

                    cAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAG‑AAAAGGCCACTGTTAACACAGTGGCCtttttctttg                                                                                                                                <  2:4263531/150‑7 (MQ=18)

                    cAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGGGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTGCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGa                                                                                                                                 >  2:285385/1‑150 (MQ=255)

                          gctCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTgg                                                                                                                           <  2:5374274/150‑1 (MQ=255)

                               aTCCCGCGTCAGTGGGGCGGATTCTACTGTGTGTGGTTGGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCg                                                                                                                      <  2:205461/150‑1 (MQ=255)

                               aTCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCg                                                                                                                      >  1:6671728/1‑150 (MQ=255)

                               aTCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATAAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGAATTTTCTTGAATCTGGTTgag                                                                                                                      >  2:1328277/1‑148 (MQ=255)

                               aTCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATCCTTCTCAGCAGGCAAACAAAAAGGCCACGGTGTTAACAGTGGCCTGTTCTTGAATCTGGTTGCg                                                                                                                      >  1:8153135/1‑150 (MQ=18)

                                tCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCgg                                                                                                                     <  2:4517911/150‑1 (MQ=255)

                                tCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTGTGGTCGCGCATGCGTATCAGCAGGCAAAGAAAAAGGCCACGGTGTTAACCGGGGCCTGGTCGTGAAGCTGGTTGCgg                                                                                                                     >  2:5299432/1‑150 (MQ=18)

                                tCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTATTAGACGATATATCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGAAACTGTGTTAACAGTGGCCTTTTCTTTAATCTGGTTGCgg                                                                                                                     >  2:4840926/1‑150 (MQ=18)

                                 cccGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCggg                                                                                                                    >  2:4403149/1‑150 (MQ=255)

                                 cccGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGAGAATGCTTATGAGGATGCTAAGAGAGTGGGCAAGGTGATGAGTGTGGCCGTTTGGTGAGTCTTGGTGCggg                                                                                                                    >  2:8438660/1‑150 (MQ=9)

                                   cgcgTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAg                                                                                                                  >  2:2379946/1‑150 (MQ=255)

                                          gTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGAtt                                                                                                           <  1:5768898/150‑1 (MQ=255)

                                           tGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTCGGCTTTGATTTTTCGACGTTTTTTCGCGACTGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTACCAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGAttt                                                                                                          <  2:3177250/150‑1 (MQ=255)

                                           tGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGAttt                                                                                                          >  1:493624/1‑150 (MQ=255)

                                           tGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGAttt                                                                                                          >  2:1699410/1‑150 (MQ=255)

                                           tGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGAttt                                                                                                          >  1:1581811/1‑150 (MQ=255)

                                           tGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGAttt                                                                                                          <  1:6904208/150‑1 (MQ=255)

                                             gggCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGa                                                                                                        <  2:227951/150‑1 (MQ=255)

                                              ggCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGaa                                                                                                       <  2:5103691/150‑1 (MQ=255)

                                              ggCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTAGTTCGCGAATGATTATGAGCAGGCAAAGAAAAAGGCCACTGTGTTTACAGTGGCGTTCTCTTGAATCTGGTTGCGGGAGCCGGATTTGaa                                                                                                       <  2:8785536/150‑1 (MQ=18)

                                               gCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAAc                                                                                                      <  1:7543123/150‑1 (MQ=255)

                                               gCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAAc                                                                                                      >  2:5164832/1‑150 (MQ=255)

                                                  gATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACcga                                                                                                   <  2:4048375/150‑1 (MQ=255)

                                                      cTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATTCTTATCAGCAGGCAAAGAAAATGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCgacgac                                                                                               <  2:2502393/150‑1 (MQ=255)

                                                      cTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGAGGGAGCCGGATTTGAACCgacgac                                                                                               >  1:7936933/1‑150 (MQ=255)

                                                       tACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGAcc                                                                                              >  1:1659937/1‑150 (MQ=255)

                                                       tACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGAcc                                                                                              >  2:2202913/1‑150 (MQ=255)

                                                        aCAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCt                                                                                             >  1:5253714/1‑150 (MQ=255)

                                                           gtgtgtGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCg                                                                                          >  1:7143434/1‑150 (MQ=255)

                                                            tgtgtgGTTTGTATGTCAAGCGCTTGGCCTTGATTTTTCGACG‑TTTTTCGCGAATGCTTATCAGCAGGCAAAGAATAAGGCATCTGTGTTAACAGTTGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCggg                                                                                        <  1:483074/150‑1 (MQ=18)

                                                            tgtgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                         >  1:114273/1‑150 (MQ=255)

                                                            tgtgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                         <  2:6603533/150‑1 (MQ=255)

                                                             gtgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCggg                                                                                        <  1:2830296/150‑1 (MQ=255)

                                                               gtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTACTTGAATCTGGTTGCGGGAGCCGGATTTCAACCGACGACCTTCGGGtt                                                                                      >  1:4025169/1‑150 (MQ=255)

                                                               gtgGTTTGTATGTCAAGCGCTAGGCTTTGATTGTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGtt                                                                                      <  2:2627341/150‑1 (MQ=255)

                                                                tgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTa                                                                                     <  1:5124796/150‑1 (MQ=255)

                                                                tgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTa                                                                                     <  2:6486800/150‑1 (MQ=255)

                                                                 ggTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTAt                                                                                    <  2:7378816/150‑1 (MQ=255)

                                                                 ggTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTAt                                                                                    >  2:4690791/1‑150 (MQ=255)

                                                                    ttGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAg                                                                                 >  1:1873047/1‑150 (MQ=255)

                                                                         tGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCGTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcga                                                                            >  2:1378905/1‑150 (MQ=255)

                                                                          gTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcgac                                                                           <  2:4219355/150‑1 (MQ=255)

                                                                                  cTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTAcc                                                                   >  2:1521108/1‑150 (MQ=255)

                                                                                   tAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCa                                                                  >  2:7852016/1‑150 (MQ=255)

                                                                                      gCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgc                                                               <  2:5253714/150‑1 (MQ=255)

                                                                                        tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgctg                                                             >  2:7112771/1‑150 (MQ=255)

                                                                                        tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgctg                                                             >  1:4444762/1‑150 (MQ=255)

                                                                                        tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgctg                                                             >  1:6401283/1‑150 (MQ=255)

                                                                                           gATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTc                                                          <  2:3990637/150‑1 (MQ=255)

                                                                                             tttttCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCa                                                        >  2:5503468/1‑150 (MQ=255)

                                                                                              ttttCGACGTTATTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCAt                                                       >  1:4178379/1‑150 (MQ=255)

                                                                                                ttCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATcc                                                     >  2:1124642/1‑150 (MQ=255)

                                                                                                   gACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAATGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATTAGCCCGACGAGCTACCAGGCTGCTCCATCCcgc                                                  >  1:2526078/1‑150 (MQ=255)

                                                                                                   gACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcgacga                                                                         <  1:8417701/127‑1 (MQ=255)

                                                                                                   gACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCcgc                                                  >  1:7753514/1‑150 (MQ=255)

                                                                                                   gACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCcgc                                                  >  2:3431519/1‑150 (MQ=255)

                                                                                                   gACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCcgc                                                  >  1:3729435/1‑150 (MQ=255)

                                                                                                         ttttCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGt                                            <  1:2234321/150‑1 (MQ=255)

                                                                                                           ttCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTgg                                          >  1:5640140/1‑150 (MQ=255)

                                                                                                              gcgAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTCCCAGGCTGCTCCATCACGCGTCAGGGGGGc                                       >  1:5995543/1‑150 (MQ=255)

                                                                                                              gcgAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGc                                       >  1:2418579/1‑150 (MQ=255)

                                                                                                              gcgAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGc                                       <  2:6046023/150‑1 (MQ=255)

                                                                                                               cgAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGATCCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCg                                      <  2:617214/150‑1 (MQ=255)

                                                                                                               cgAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCg                                      >  1:8537326/1‑150 (MQ=255)

                                                                                                                gAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGGCAGTGGGGCgg                                     >  1:861886/1‑150 (MQ=255)

                                                                                                                      ttATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCTGGTTATGAGCCCGACGAGCTACCTGGCTGGTCCAGCCCTCTTCACTGGTGGGGAGTcga                               >  1:1475331/1‑148 (MQ=14)

                                                                                                                      ttATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCtc                               >  1:1967312/1‑149 (MQ=255)

                                                                                                                      ttATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTa                               >  1:8707127/1‑150 (MQ=255)

                                                                                                                      ttATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCAGATCCATACCGCGACAGTCGGGCGGaaacta                               >  1:2639618/1‑145 (MQ=14)

                                                                                                                                  aaaGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAg                                                                                 >  2:2763993/1‑88 (MQ=38)

                                                                                                                                  aaaGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATc                                                      <  1:315589/115‑1 (MQ=35)

                                                                                                                                   aaGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGttt                  <  1:3999904/150‑1 (MQ=255)

                                                                                                                                     gAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcgac                                                                           >  2:3042157/1‑91 (MQ=37)

                                                                                                                                      gaaaaGGCCACTGTTAACACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCGTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCGCCATCCCGCGTCCGTGGGGCGGATTCTACAGCGTGTAGTTtata               >  1:1174822/2‑150 (MQ=17)

                                                                                                                                          aGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTc           <  1:7049407/150‑1 (MQ=255)

                                                                                                                                          aGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTc           >  2:3792254/1‑150 (MQ=255)

                                                                                                                                           ggCCACTGTTAACACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCAGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCa          >  1:8060629/1‑150 (MQ=17)

                                                                                                                                           ggCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGACTGTGGGGCGGAATCAACAGCGTGTAGTGAATATGTCa          >  1:1385085/1‑150 (MQ=17)

                                                                                                                                             ccACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcgacga                                                                         <  2:7055744/85‑1 (MQ=38)

                                                                                                                                             ccACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcgacga                                                                         >  2:7625105/1‑85 (MQ=38)

                                                                                                                                              cACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCcg                                                   >  2:8197129/1‑106 (MQ=35)

                                                                                                                                                cTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGa                                                                                  >  2:8621138/1‑73 (MQ=39)

                                                                                                                                                 tgtgTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCt    <  1:285385/150‑1 (MQ=255)

TTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTAG  >  NC_002947/1385529‑1385825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`