BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,385,680	A→C	6.1%	intergenic (‑113/+22)	PP_t67 ← / ← PP_t66	tRNA‑Met/tRNA‑Met

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,385,680	0	A	C	6.1%	1157.6 / 42.7	361	intergenic (‑113/+22)	PP_t67/PP_t66	tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand): ref base A (184/150); new base C (4/19); total (190/171)
Fisher's exact test for biased strand distribution p-value = 4.36e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 364 total aligned reads displayed.

TGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTAGGCTT  >  NC_002947/1385532‑1385829

tGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCTGTGGGGCGGATTCTACATTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTaa                                                                                                                                                      <  2:6367256/150‑1 (MQ=255)

  aGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACa                                                                                                                                                    <  1:4670118/150‑1 (MQ=255)

    cccGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGt                                                                                                                                                  <  2:1053573/150‑1 (MQ=255)

      cgacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTgg                                                                                                                                                <  2:1942030/150‑1 (MQ=255)

       gacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGc                                                                                                                                               <  2:5923901/150‑1 (MQ=255)

            gCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAGGGCCACTGTTAACACAGTGGCCtttt                                                                                                                                          <  1:458468/150‑1 (MQ=18)

            gCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCtttt                                                                                                                                          <  1:3021554/150‑1 (MQ=255)

            gCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCtttt                                                                                                                                          <  1:2366062/150‑1 (MQ=255)

             cTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTc                                                                                                                                         <  1:2049422/150‑1 (MQ=255)

             cTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTGTTCGCGAATGGTTATAGGCAGGCAATGAAATAGGCAACTGTGTTACGAGTGGCCTTTTc                                                                                                                                         >  2:792972/1‑150 (MQ=18)

              tACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTCAACAGTGGCCTTTTCt                                                                                                                                        <  1:8799118/150‑1 (MQ=255)

               aCCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCtt                                                                                                                                       >  1:6046023/1‑150 (MQ=255)

                 cAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAG‑AAAAGGCCACTGTTAACACAGTGGCCtttttctttg                                                                                                                                    <  2:4263531/150‑7 (MQ=18)

                 cAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGGGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTGCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGa                                                                                                                                     >  2:285385/1‑150 (MQ=255)

                       gctCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTgg                                                                                                                               <  2:5374274/150‑1 (MQ=255)

                          ccATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACCTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTg                                                                                                                            >  2:6893460/1‑150 (MQ=255)

                            aTCCCGCGTCAGTGGGGCGGATTCTACTGTGTGTGGTTGGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCg                                                                                                                          <  2:205461/150‑1 (MQ=255)

                            aTCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATAAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGAATTTTCTTGAATCTGGTTgag                                                                                                                          >  2:1328277/1‑148 (MQ=255)

                            aTCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATCCTTCTCAGCAGGCAAACAAAAAGGCCACGGTGTTAACAGTGGCCTGTTCTTGAATCTGGTTGCg                                                                                                                          >  1:8153135/1‑150 (MQ=18)

                            aTCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATCTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCg                                                                                                                          >  1:6671758/1‑150 (MQ=255)

                             tCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCgg                                                                                                                         <  2:4517911/150‑1 (MQ=255)

                             tCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCgg                                                                                                                         >  2:7496178/1‑150 (MQ=255)

                             tCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTGTGGTCGCGCATGCGTATCAGCAGGCAAAGAAAAAGGCCACGGTGTTAACCGGGGCCTGGTCGTGAAGCTGGTTGCgg                                                                                                                         >  2:5299432/1‑150 (MQ=18)

                             tCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTATTAGACGATATATCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGAAACTGTGTTAACAGTGGCCTTTTCTTTAATCTGGTTGCgg                                                                                                                         >  2:4840926/1‑150 (MQ=18)

                              cccGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCggg                                                                                                                        >  2:4403149/1‑150 (MQ=255)

                              cccGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGAGAATGCTTATGAGGATGCTAAGAGAGTGGGCAAGGTGATGAGTGTGGCCGTTTGGTGAGTCTTGGTGCggg                                                                                                                        >  2:8438660/1‑150 (MQ=9)

                                cgcgTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAg                                                                                                                      >  2:2379946/1‑150 (MQ=255)

                                       gTGGGGCGGATTCTACAGTGTGTGGTTTGCATGTCAAGCGCTAGGCTTTGATTTTTCGACTTTTTTTCGCGTATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTCACAGTGGCCTTTTCTAGAATCTGGTTGCGGGAGCCGGAtt                                                                                                               <  1:5768678/150‑1 (MQ=18)

                                        tGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTCGGCTTTGATTTTTCGACGTTTTTTCGCGACTGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTACCAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGAttt                                                                                                              <  2:3177250/150‑1 (MQ=255)

                                        tGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGAttt                                                                                                              >  1:493624/1‑150 (MQ=255)

                                        tGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGAttt                                                                                                              >  2:1699410/1‑150 (MQ=255)

                                        tGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGAttt                                                                                                              >  1:1581811/1‑150 (MQ=255)

                                        tGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGAttt                                                                                                              <  1:6904208/150‑1 (MQ=255)

                                          gggCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGa                                                                                                            >  1:4278716/1‑150 (MQ=255)

                                          gggCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGa                                                                                                            <  2:227951/150‑1 (MQ=255)

                                           ggCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGaa                                                                                                           <  2:5103691/150‑1 (MQ=255)

                                           ggCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTAGTTCGCGAATGATTATGAGCAGGCAAAGAAAAAGGCCACTGTGTTTACAGTGGCGTTCTCTTGAATCTGGTTGCGGGAGCCGGATTTGaa                                                                                                           <  2:8785536/150‑1 (MQ=18)

                                           ggCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCCCTACCCTTTGATTTTTCTACCTTTTTTCGCTAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGaa                                                                                                           <  2:3794578/150‑1 (MQ=18)

                                            gCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAAc                                                                                                          <  1:7543123/150‑1 (MQ=255)

                                            gCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAAc                                                                                                          >  2:5164832/1‑150 (MQ=255)

                                            gCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAAc                                                                                                          <  1:5826437/150‑1 (MQ=255)

                                               gATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACcga                                                                                                       <  2:4048375/150‑1 (MQ=255)

                                                   cTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATTCTTATCAGCAGGCAAAGAAAATGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCgacgac                                                                                                   <  2:2502393/150‑1 (MQ=255)

                                                   cTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGAGGGAGCCGGATTTGAACCgacgac                                                                                                   >  1:7936933/1‑150 (MQ=255)

                                                    tACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGAcc                                                                                                  >  1:1659937/1‑150 (MQ=255)

                                                    tACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGAcc                                                                                                  >  2:2202913/1‑150 (MQ=255)

                                                        gtgtgtGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCg                                                                                              >  1:7143434/1‑150 (MQ=255)

                                                         tgtgtgGTTTGTATGTCAAGCGCTTGGCCTTGATTTTTCGACG‑TTTTTCGCGAATGCTTATCAGCAGGCAAAGAATAAGGCATCTGTGTTAACAGTTGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCggg                                                                                            <  1:483074/150‑1 (MQ=18)

                                                         tgtgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                             >  1:114273/1‑150 (MQ=255)

                                                         tgtgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                             <  2:6603533/150‑1 (MQ=255)

                                                          gtgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCggg                                                                                            <  1:2830296/150‑1 (MQ=255)

                                                            gtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGtt                                                                                          <  2:3529393/150‑1 (MQ=255)

                                                            gtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTACTTGAATCTGGTTGCGGGAGCCGGATTTCAACCGACGACCTTCGGGtt                                                                                          >  1:4025169/1‑150 (MQ=255)

                                                            gtgGTTTGTATGTCAAGCGCTAGGCTTTGATTGTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGtt                                                                                          <  2:2627341/150‑1 (MQ=255)

                                                             tgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTa                                                                                         <  1:5124796/150‑1 (MQ=255)

                                                             tgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTa                                                                                         <  2:6486800/150‑1 (MQ=255)

                                                              ggTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTAt                                                                                        >  2:4690791/1‑150 (MQ=255)

                                                               gTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATg                                                                                       >  2:3301738/1‑150 (MQ=255)

                                                               gTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATg                                                                                       >  2:7045756/1‑150 (MQ=255)

                                                                 ttGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAg                                                                                     >  1:1873047/1‑150 (MQ=255)

                                                                      tGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCGTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcga                                                                                >  2:1378905/1‑150 (MQ=255)

                                                                       gTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcgac                                                                               <  2:4219355/150‑1 (MQ=255)

                                                                           aGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGc                                                                           <  1:5275677/150‑1 (MQ=255)

                                                                               cTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTAcc                                                                       >  2:1521108/1‑150 (MQ=255)

                                                                                tAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCa                                                                      >  2:7852016/1‑150 (MQ=255)

                                                                                   gCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgc                                                                   <  2:5253714/150‑1 (MQ=255)

                                                                                     tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgctg                                                                 >  2:7112507/1‑150 (MQ=255)

                                                                                     tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgctg                                                                 >  1:4444762/1‑150 (MQ=255)

                                                                                        gATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTc                                                              <  2:3990637/150‑1 (MQ=255)

                                                                                          tttttCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCa                                                            >  2:5503468/1‑150 (MQ=255)

                                                                                           ttttCGACGTTATTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCAt                                                           >  1:4178379/1‑150 (MQ=255)

                                                                                             ttCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATcc                                                         >  2:1124642/1‑150 (MQ=255)

                                                                                                gACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAATGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATTAGCCCGACGAGCTACCAGGCTGCTCCATCCcgc                                                      >  1:2526078/1‑150 (MQ=255)

                                                                                                gACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTAt                                                                                        <  2:723731/116‑1 (MQ=255)

                                                                                                gACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcgacga                                                                             <  1:8417701/127‑1 (MQ=255)

                                                                                                gACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCcgc                                                      >  1:7753514/1‑150 (MQ=255)

                                                                                                gACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCcgc                                                      >  1:3729435/1‑150 (MQ=255)

                                                                                                      ttttCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGt                                                <  1:2234321/150‑1 (MQ=255)

                                                                                                           gcgAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGc                                           >  1:2418579/1‑150 (MQ=255)

                                                                                                           gcgAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGc                                           <  2:6046023/150‑1 (MQ=255)

                                                                                                            cgAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGATCCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCg                                          <  2:617214/150‑1 (MQ=255)

                                                                                                            cgAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCg                                          >  1:8537326/1‑150 (MQ=255)

                                                                                                             gAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGGCAGTGGGGCgg                                         >  1:861886/1‑150 (MQ=255)

                                                                                                                   ttATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCTGGTTATGAGCCCGACGAGCTACCTGGCTGGTCCAGCCCTCTTCACTGGTGGGGAGTcga                                   >  1:1475331/1‑148 (MQ=14)

                                                                                                                   ttATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCtc                                   >  1:1967312/1‑149 (MQ=255)

                                                                                                                   ttATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTa                                   >  1:8707127/1‑150 (MQ=255)

                                                                                                                   ttATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCAGATCCATACCGCGACAGTCGGGCGGaaacta                                   >  1:2639618/1‑145 (MQ=14)

                                                                                                                               aaaGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAg                                                                                     >  2:2763993/1‑88 (MQ=38)

                                                                                                                               aaaGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATc                                                          <  1:315589/115‑1 (MQ=35)

                                                                                                                               aaaGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGtt                       >  2:7602725/1‑150 (MQ=255)

                                                                                                                                aaGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGttt                      <  1:3999904/150‑1 (MQ=255)

                                                                                                                                  gAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcgac                                                                               >  2:3042157/1‑91 (MQ=37)

                                                                                                                                   gaaaaGGCCACTGTTAACACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCGTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCGCCATCCCGCGTCCGTGGGGCGGATTCTACAGCGTGTAGTTtata                   >  1:1174822/2‑150 (MQ=17)

                                                                                                                                       aGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTc               <  1:7049407/150‑1 (MQ=255)

                                                                                                                                        ggCCACTGTTAACACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCAGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCa              >  1:8060629/1‑150 (MQ=17)

                                                                                                                                        ggCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGACTGTGGGGCGGAATCAACAGCGTGTAGTGAATATGTCa              >  1:1385085/1‑150 (MQ=17)

                                                                                                                                           cACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCcg                                                       >  2:8197129/1‑106 (MQ=35)

                                                                                                                                             cTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGa                                                                                      >  2:8621138/1‑73 (MQ=39)

                                                                                                                                              tgtgTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCt        <  1:285385/150‑1 (MQ=255)

                                                                                                                                                   tAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcgacga                                                                             >  1:6540178/1‑76 (MQ=34)

TGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTAGGCTT  >  NC_002947/1385532‑1385829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`