BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,453,168	T→A	5.5%	L48Q (CTG→CAG)	PP_1265 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,453,168	0	T	A	5.5%	1363.8 / 42.1	403	L48Q (CTG→CAG)	PP_1265	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (183/197); new base A (14/9); total (197/206)
Fisher's exact test for biased strand distribution p-value = 2.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 418 total aligned reads displayed.

TTGCACCATGCCCCGTGAGATCGCCTTCCATGGCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGTGCTGGTCGCTGCCGTGGCCATCGGTCGCCAGTTGTGGCTGCAC  >  NC_002947/1453019‑1453317

  gCACCATGCCCCGTGAGATCGCCTTCCATGGCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCtgctgc                                                                                                                                                     <  2:258114/150‑1 (MQ=255)

    aCCATGCCCCGTGAGATCGCCTTCCATGGCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTgcgc                                                                                                                                                   <  1:7557863/150‑1 (MQ=255)

      cATGCCCCGTGAGATCGCCTTCCATGGCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCt                                                                                                                                                 <  1:3207237/150‑1 (MQ=255)

       aTGCCCCGTGAGATCGCCTTCCATGGCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCAGCAGCGccgg                                                                                                                                                <  1:1207316/150‑3 (MQ=255)

        tGCCCCGTGAGATCGCCTTCCATGGCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGa                                                                                                                                               <  1:7091027/150‑1 (MQ=255)

         gCCCCGTGAGATCGCCTTCCATGGCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAg                                                                                                                                              <  1:2577124/150‑1 (MQ=255)

         gCCCCGTGAGATCGCCTTCCATGGCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCAGCAGCGccgggt                                                                                                                                              <  2:2860373/150‑5 (MQ=255)

         gCCCCGTGAGATCGCCTTCCATGGCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCAGCAGCGccgggt                                                                                                                                              <  1:5088513/150‑5 (MQ=255)

         gCCCCGTGAGATCGCCTTCCATGGCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCAGTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCAGCAGCGccgggt                                                                                                                                              <  2:7303041/150‑5 (MQ=255)

             cGTGAGATCGCCTTCCATGGCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTg                                                                                                                                          >  2:6712226/1‑150 (MQ=255)

             cGTGAGATCGCCTTCCATGGCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTg                                                                                                                                          >  1:3404924/1‑150 (MQ=255)

                gagaTCGCCTTCCATGGCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTc                                                                                                                                       <  1:5404095/150‑1 (MQ=255)

                   aTCGCCTTCCATGGCGTGTACATGCCCACCATCTCCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTc                                                                                                                                    >  2:5398142/1‑150 (MQ=255)

                    tCGCCTTCCATGGCGTGTACATGCCAACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCGGGGGCCTGGACCGGTTAATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTcg                                                                                                                                   >  1:5544762/1‑149 (MQ=255)

                     cGCCTTCCATGGCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTg                                                                                                                                  >  1:6561157/1‑150 (MQ=255)

                     cGCCTTCCATGGCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTg                                                                                                                                  <  1:1038856/150‑1 (MQ=255)

                         ttCCATGGCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTg                                                                                                                              >  1:1894153/1‑150 (MQ=255)

                          tCCATGGCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGt                                                                                                                             <  1:8532300/150‑1 (MQ=255)

                          tCCATGGCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGt                                                                                                                             <  1:4941884/150‑1 (MQ=255)

                           ccATGGCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGc                                                                                                                                 >  2:4504145/1‑145 (MQ=255)

                           ccATGGCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGtt                                                                                                                            >  1:5787192/1‑150 (MQ=255)

                           ccATGGCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGtt                                                                                                                            >  1:1105114/1‑150 (MQ=255)

                            cATGGCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTc                                                                                                                           <  1:2338718/150‑1 (MQ=255)

                               ggCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGgc                                                                                                                        <  2:7575330/150‑1 (MQ=255)

                                gCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGgcg                                                                                                                       >  2:3468868/1‑150 (MQ=255)

                                   tgtACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCa                                                                                                                    <  1:2990058/150‑1 (MQ=255)

                                        aTGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGgcg                                                                                                               >  2:5537320/1‑150 (MQ=255)

                                           cccACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGctg                                                                                                            >  1:4491126/1‑150 (MQ=255)

                                            ccaccaTGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGctgt                                                                                                           <  1:4885170/150‑1 (MQ=255)

                                            ccaccaTGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGctgt                                                                                                           <  1:5882759/150‑1 (MQ=255)

                                            ccaccaTGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGctgt                                                                                                           <  2:7268472/150‑1 (MQ=255)

                                              accaTGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGctgtct                                                                                                         <  2:5036585/150‑1 (MQ=255)

                                              accaTGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGctgtct                                                                                                         >  2:1906758/1‑150 (MQ=255)

                                                caTGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGctgtctgt                                                                                                       <  2:5803952/150‑1 (MQ=255)

                                                    aCCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTAc                                                                                                   <  2:2683501/150‑1 (MQ=255)

                                                      cTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTg                                                                                                 >  2:5981707/1‑150 (MQ=255)

                                                      cTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTACGGCGCCATGGCGCTGTCTGACTACTg                                                                                                 >  1:2892167/1‑150 (MQ=255)

                                                      cTTGATGTTCCTGTTTGCCCAGGGCCAGGCCTGGGGCCTGGACAGGTTCATTGCCAGCCATGATGGCTACAGCTACTACTGGCACCCTGCGCTGCTGCGCCTGAGCATGTTCGTCTGCTTGTTCGGCGCCATCGCGCTGTCTGACTACTg                                                                                                 >  2:7513920/1‑150 (MQ=255)

                                                       ttGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTgg                                                                                                >  2:6821769/1‑150 (MQ=255)

                                                        tGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGt                                                                                               <  1:5012087/150‑1 (MQ=255)

                                                        tGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGt                                                                                               <  2:4350419/150‑1 (MQ=255)

                                                          aTGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTga                                                                                             <  2:5650445/150‑1 (MQ=255)

                                                          aTGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTga                                                                                             <  2:4139139/150‑1 (MQ=255)

                                                          aTGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTga                                                                                             <  1:4265841/150‑1 (MQ=255)

                                                           tGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTgag                                                                                            >  2:489815/1‑150 (MQ=255)

                                                                  gTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCtt                                                                                     <  1:4776917/150‑1 (MQ=255)

                                                                  gTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCtt                                                                                     <  1:6670837/150‑1 (MQ=255)

                                                                  gTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCtt                                                                                     <  2:7711061/150‑1 (MQ=255)

                                                                   tttGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTc                                                                                    <  1:8173011/150‑1 (MQ=255)

                                                                        ccTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGa                                                                               >  2:221098/1‑150 (MQ=255)

                                                                           gggCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGaaaa                                                                            >  1:2126197/1‑150 (MQ=255)

                                                                            ggcctggcctGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGTCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGaaaaa                                                                           <  2:7639607/150‑1 (MQ=255)

                                                                            ggcctggcctGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGaaaaa                                                                           <  2:2090189/150‑1 (MQ=255)

                                                                              cctggcctggGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGctgt                                                                                                           <  1:418019/116‑1 (MQ=255)

                                                                               ctggcctggGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGtt                                                                        >  2:4744758/1‑150 (MQ=255)

                                                                                tggcctggGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGttc                                                                       >  1:7793190/1‑150 (MQ=255)

                                                                                tggcctggGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGttc                                                                       >  2:4243789/1‑150 (MQ=255)

                                                                                tggcctggGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGttc                                                                       >  2:130897/1‑150 (MQ=255)

                                                                                tggcctggGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGttc                                                                       >  1:7336668/1‑150 (MQ=255)

                                                                                tggcctggGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCAGGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGttc                                                                       <  1:2912955/150‑1 (MQ=255)

                                                                                   cctggggcctggACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGttcttc                                                                    <  2:7156672/150‑1 (MQ=255)

                                                                                    ctggggcctggACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCa                                                                   <  2:3534650/150‑1 (MQ=255)

                                                                                        ggcctggACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCt                                                               <  1:3749640/150‑1 (MQ=255)

                                                                                         gcctggACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTg                                                              >  2:3877858/1‑150 (MQ=255)

                                                                                            tggACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATc                                                           <  1:7294389/150‑1 (MQ=255)

                                                                                             ggACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCg                                                          >  1:8026547/1‑150 (MQ=255)

                                                                                               aCCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCAcc                                                        >  1:6021549/1‑150 (MQ=255)

                                                                                                ccGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCGTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCa                                                       >  2:3030827/1‑150 (MQ=255)

                                                                                                   gTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCAccc                                                    >  2:3772104/1‑150 (MQ=255)

                                                                                                     tCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGGCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTGCGATGAAAATGTTCTTCTGCCTGATCGCCCCCctg                                                  >  2:1598857/1‑150 (MQ=255)

                                                                                                      cATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCctgc                                                 >  1:8373780/1‑150 (MQ=255)

                                                                                                        ttGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCctgctg                                               <  1:2236733/150‑1 (MQ=255)

                                                                                                            cagccaTGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCtgctggtgc                                           >  1:6981624/1‑150 (MQ=255)

                                                                                                            cagccaTGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCtgctggtgc                                           >  2:6240440/1‑150 (MQ=255)

                                                                                                              gccaTGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCtgctggtgctg                                         <  1:2653560/150‑1 (MQ=255)

                                                                                                               ccaTGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCtgctggtgctgg                                        >  1:7186514/1‑150 (MQ=255)

                                                                                                                   gatgGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGTGCTGGTCGc                                    <  2:887517/150‑1 (MQ=255)

                                                                                                                   gatgGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGTGCTGGTCGc                                    >  2:4213383/1‑150 (MQ=255)

                                                                                                                        cTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATACTTCGATGAAAAAGTTCTTCATCCTGATCGCCACCCTGCTGGTGCTGGTCTCTGCCg                               <  2:8035100/150‑1 (MQ=255)

                                                                                                                         tACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGTGCTGGTCGCTGCCGt                              >  2:1686408/1‑150 (MQ=255)

                                                                                                                           ccGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGTGCTGGTCGCTGCCGTgg                            <  1:4316557/150‑1 (MQ=255)

                                                                                                                           ccGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGTGCTGGTCGCTGCCGTgg                            >  1:7530534/1‑150 (MQ=255)

                                                                                                                            cGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGTGCTGGTCGCTGCCGTGGc                           <  2:642684/150‑1 (MQ=255)

                                                                                                                            cGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGTGCTGGTCGCTGCCGTGGc                           <  1:683108/150‑1 (MQ=255)

                                                                                                                                 cttctGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGTGCTGGTCGCTGCCGTGGCCATCg                      >  1:1644992/1‑150 (MQ=255)

                                                                                                                                 cttctGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGTGCTGGTCGCTGCCGTGGCCATCg                      >  1:4640821/1‑150 (MQ=255)

                                                                                                                                 cttctGGCACCCGGCGCCGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTAGTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGTGCTGGTCGCTGCCGTGGCCAGCg                      >  2:2254293/1‑150 (MQ=255)

                                                                                                                                  ttctGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGTGCTGGTCGCTGCCGTGGCCATCgg                     >  1:2488771/1‑150 (MQ=255)

                                                                                                                                    ctGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGTGCTGGTCGCTGCCGTGGCCATCGGTc                   <  1:1376374/150‑1 (MQ=255)

                                                                                                                                         aCCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCTATGAAAAAGTTCTTCAGCCTGATCTCCACACTGCTGGTGCTGGTCGCTGCCGAGGCCATCGGTCGCCAg              >  1:3623803/1‑150 (MQ=255)

                                                                                                                                         aCCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGTGCTGGTCGCTGCCGTGGCCATCGGTCGCCAg              >  2:3975433/1‑150 (MQ=255)

                                                                                                                                          tcaggCGCAGCAGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGTGCTGGTCGCTGCCGTGGCCATCGGTCGCCAGt             >  1:8758809/4‑150 (MQ=255)

                                                                                                                                          tcaggCGCAGCAGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGAGCTGGTCGCTGCCGTGGCCATCGGTCGCCAGt             >  2:2403620/4‑150 (MQ=255)

                                                                                                                                           ccGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGTGCTGGTCGCTGCCGTGGCCATCGGTCGCCAGtt            >  2:6997002/1‑150 (MQ=255)

                                                                                                                                           ccGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGTGCTGGTCGCTGCCGTGGCCATCGGTCGCCAGtt            >  2:789208/1‑150 (MQ=255)

                                                                                                                                             ggCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGTGCTGGTCGCTGCCGTGGCCATCGGTCGCCAGTtgt          >  2:8156405/1‑150 (MQ=255)

                                                                                                                                             ggCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGTGCTGGTCGCTGCCGTGGCCATCGGTCGCCAGTtgt          >  2:8470285/1‑150 (MQ=255)

                                                                                                                                              gcgcTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGTGCTGGTCGCTGCCGTGGCCATCGGTCGCCAGTtgtg         <  2:3661952/150‑1 (MQ=255)

                                                                                                                                              gcgcTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCAGGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGTGCTGGTCGCTGCCGTGGCCATCGGTCGCCAGTtgtg         <  1:6337708/150‑1 (MQ=255)

                                                                                                                                                  tgctgcGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGTGCTGGTCGCTGCCGTGGCCATCGGTCGCCAGTTGTGGCTg     >  1:9489/1‑150 (MQ=255)

TTGCACCATGCCCCGTGAGATCGCCTTCCATGGCGTGTACATGCCCACCATGACCTTGATGTTCCTGTTTGCCCTGGGCCTGGCCTGGGGCCTGGACCGGTTCATTGCCAGCCATGATGGCTACCGCTTCTTCTGGCACCCGGCGCTGCTGCGCCTGAGCCTGTTCGTCTGCTTGTTCGGCGCCATGGCGCTGTCTGTCTACTGGTGAGAATCCTTCGATGAAAAAGTTCTTCAGCCTGATCGCCACCCTGCTGGTGCTGGTCGCTGCCGTGGCCATCGGTCGCCAGTTGTGGCTGCAC  >  NC_002947/1453019‑1453317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`