BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,493,005	T→A	6.0%	N92Y (AAC→TAC)	degS ←	serine endoprotease DegS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,493,005	0	T	A	6.0%	1354.4 / 49.1	403	N92Y (AAC→TAC)	degS	serine endoprotease DegS
Reads supporting (aligned to +/- strand): ref base T (168/210); new base A (16/8); total (185/218)
Fisher's exact test for biased strand distribution p-value = 5.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 411 total aligned reads displayed.

GGTGACATGGTTGTTGGTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAA  >  NC_002947/1492856‑1493154

  tGACATGGTTGTTGGTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTca                                                                                                                                                     <  2:3217283/150‑1 (MQ=255)

   gACATGGTTGTTGGTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTcac                                                                                                                                                    >  2:3062709/1‑150 (MQ=255)

    aCATGGTTGTTGGTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTcacc                                                                                                                                                   >  1:6541882/1‑150 (MQ=255)

      aTGGTTGTTGGTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTcaccac                                                                                                                                                 >  2:8723940/1‑150 (MQ=255)

      aTGGTTGTTGGTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTcaccac                                                                                                                                                 >  2:1703947/1‑150 (MQ=255)

      aTGGTTGTTGGTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAAAAGCGGGTGGGCGCTTTTGTTcaccac                                                                                                                                                 >  1:8299315/1‑150 (MQ=255)

         gttgttGGTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCtt                                                                                                                                              >  1:7117377/1‑150 (MQ=255)

           tgttgGTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTtgg                                                                                                                                            <  2:1101305/150‑1 (MQ=255)

                gTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGgtggtg                                                                                                                                       <  1:4895880/150‑1 (MQ=255)

                gTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGgtggtg                                                                                                                                       <  2:2587924/150‑1 (MQ=255)

                gTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGgtggtg                                                                                                                                       <  2:4693030/150‑1 (MQ=255)

                 tCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGgtgt                                                                                                                                      <  2:7532032/150‑1 (MQ=255)

                  cagcagGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCAAGgtggtgaa                                                                                                                                     <  1:3465835/150‑3 (MQ=255)

                   agcagGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTAc                                                                                                                                    >  2:7400271/1‑150 (MQ=255)

                   agcagGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTAc                                                                                                                                    >  2:7864836/1‑150 (MQ=255)

                   agcagGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTAc                                                                                                                                    >  1:4431864/1‑150 (MQ=255)

                    gcagGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACa                                                                                                                                   <  1:1680679/150‑1 (MQ=255)

                     cagGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAg                                                                                                                                  <  1:3257496/150‑1 (MQ=255)

                      agGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAgg                                                                                                                                 >  1:7340583/1‑150 (MQ=255)

                       ggTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTACACCACCAAGGTGGTGAACaaaa                                                                                                                                <  1:6705639/150‑4 (MQ=255)

                         tAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTg                                                                                                                              <  1:114622/150‑1 (MQ=255)

                           gCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGc                                                                                                                            <  1:333802/150‑1 (MQ=255)

                           gCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGc                                                                                                                            <  2:622275/150‑1 (MQ=255)

                              ctctGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCAc                                                                                                                         >  1:5785126/1‑150 (MQ=255)

                               tctGGGCTCATGATTACCGCCGAACCCAGGCTGGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCAcc                                                                                                                        >  2:5599410/1‑150 (MQ=255)

                                  gggCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCAccgcc                                                                                                                     <  1:6858321/150‑1 (MQ=255)

                                    gCTCATGATTACCGCCGAACCCAGGCTTGAGTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCgg                                                                                                                   <  2:5475130/150‑1 (MQ=255)

                                      tCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCgggg                                                                                                                 >  2:4047601/1‑150 (MQ=255)

                                      tCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCgggg                                                                                                                 >  1:6985598/1‑150 (MQ=255)

                                      tCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCgggg                                                                                                                 >  2:2664073/1‑150 (MQ=255)

                                       cATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGggc                                                                                                                <  1:4068773/150‑1 (MQ=255)

                                       cATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGggc                                                                                                                <  1:2753335/150‑1 (MQ=255)

                                        aTGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGggcg                                                                                                               >  1:8536501/1‑150 (MQ=255)

                                         tGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGggcgg                                                                                                              >  2:5139933/1‑150 (MQ=255)

                                         tGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGggcgg                                                                                                              >  1:8072516/1‑150 (MQ=255)

                                          gATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGggcggc                                                                                                             <  1:5466696/150‑1 (MQ=255)

                                            ttACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTTGCCACCGCCGGGGCGGCCa                                                                                                           <  2:689656/150‑1 (MQ=255)

                                            ttACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCa                                                                                                           <  2:2817097/150‑1 (MQ=255)

                                             tACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCTGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAg                                                                                                          <  1:5993419/150‑1 (MQ=255)

                                              aCCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCGCCCCGGCGGTGGCCaac                                                                                                         <  1:1016845/150‑3 (MQ=255)

                                              aCCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCGCCCCGGCGGTGGCCaac                                                                                                         <  2:7764265/150‑3 (MQ=255)

                                              aCCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCGCCCCGGCGGTGGCCaac                                                                                                         <  2:6363414/150‑3 (MQ=255)

                                              aCCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAgg                                                                                                         <  1:8472691/150‑1 (MQ=255)

                                              aCCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAgg                                                                                                         <  2:5808592/150‑1 (MQ=255)

                                              aCCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAgg                                                                                                         <  2:4771087/150‑1 (MQ=255)

                                                cgccgAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGt                                                                                                       >  1:5682508/1‑150 (MQ=255)

                                                  ccgAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGTTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCAACCGCCGGGGCGGCCAGGGTcc                                                                                                     >  2:1217716/1‑149 (MQ=255)

                                                   cgAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCAc                                                                                                    <  2:5996227/150‑1 (MQ=255)

                                                    gAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCAcg                                                                                                   <  2:5179075/150‑1 (MQ=255)

                                                        ccAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCAcggcgt                                                                                               >  2:3668321/1‑150 (MQ=255)

                                                         cAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCAcggcgtc                                                                                              >  1:3737826/1‑150 (MQ=255)

                                                         cAGGCTTGATTCCCAGCGGCGCGGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCAcggcgtc                                                                                              >  2:8121094/1‑150 (MQ=255)

                                                          aGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCAcggcgtcg                                                                                             <  2:3610348/150‑1 (MQ=255)

                                                          aGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCAAGGTGGTGAACa                                                                                                                                   <  2:1930994/112‑1 (MQ=255)

                                                            gCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCAcggcgtcggc                                                                                           >  1:3876367/1‑150 (MQ=255)

                                                               tGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTa                                                                                        <  2:1662575/150‑1 (MQ=255)

                                                               tGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTa                                                                                        <  2:7158376/150‑1 (MQ=255)

                                                               tGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTa                                                                                        <  1:6345322/150‑1 (MQ=255)

                                                                     ccAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGacac                                                                                  <  1:1827615/150‑1 (MQ=255)

                                                                      cAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACAcc                                                                                 >  1:7892987/1‑150 (MQ=255)

                                                                            cgcTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCt                                                                           <  1:1407696/150‑1 (MQ=255)

                                                                                    gggCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGAt                                                                   >  2:4913378/1‑150 (MQ=255)

                                                                                     ggCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATc                                                                  >  2:3315814/1‑150 (MQ=255)

                                                                                      gCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATcc                                                                 >  2:3140459/1‑150 (MQ=255)

                                                                                       cAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGGTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCg                                                                <  2:2341253/150‑1 (MQ=255)

                                                                                        aGGTTATCGCCGAAGATGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCgg                                                               <  1:1710376/150‑1 (MQ=255)

                                                                                        aGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCgg                                                               <  2:67214/150‑1 (MQ=255)

                                                                                          gTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGt                                                             >  1:317193/1‑150 (MQ=255)

                                                                                          gTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGt                                                             <  2:4510367/150‑1 (MQ=255)

                                                                                              tCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTc                                                         <  2:6559466/150‑1 (MQ=255)

                                                                                              tCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTc                                                         >  2:6999782/1‑150 (MQ=255)

                                                                                                gCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCtg                                                       >  2:134718/1‑150 (MQ=255)

                                                                                                 ccGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCtgt                                                      <  1:4718056/150‑1 (MQ=255)

                                                                                                 ccGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACAACACCGGGCCTTGCATGATCCGGGTAGTCtgt                                                      >  1:5548046/1‑150 (MQ=255)

                                                                                                  cGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCtgtg                                                     <  2:4180369/150‑1 (MQ=255)

                                                                                                  cGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCtgtg                                                     <  2:2191944/150‑1 (MQ=255)

                                                                                                         gcggcgGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCgg                                                               >  1:5043839/1‑133 (MQ=255)

                                                                                                          cggcggAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTtgc                                             <  2:860530/150‑1 (MQ=255)

                                                                                                          cggcggAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTtgc                                             <  2:920509/150‑1 (MQ=255)

                                                                                                                aaCTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAgg                                       <  1:988411/150‑1 (MQ=255)

                                                                                                                 aCTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCTGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGt                                      >  1:621403/1‑150 (MQ=255)

                                                                                                                  cTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCATGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGtt                                     <  1:761139/150‑1 (MQ=255)

                                                                                                                  cTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGtt                                     <  1:1568124/150‑1 (MQ=255)

                                                                                                                         tCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTcc                              >  1:7176118/1‑150 (MQ=255)

                                                                                                                               aaCAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCctggctg                        <  2:1040511/150‑1 (MQ=255)

                                                                                                                                actgCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGGACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACAGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCctggctgg                       <  1:439764/147‑1 (MQ=255)

                                                                                                                                aCAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCctggctgg                       <  1:5209717/150‑1 (MQ=255)

                                                                                                                                 cAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTggg                      <  1:304367/150‑1 (MQ=255)

                                                                                                                                  aGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGc                     >  1:353488/1‑150 (MQ=255)

                                                                                                                                           gcgcTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTCGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGAcc            >  2:8283554/1‑150 (MQ=255)

                                                                                                                                           gcgcTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGAcc            >  1:2450678/1‑150 (MQ=255)

                                                                                                                                           gcgcTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGAcc            <  1:8725451/150‑1 (MQ=255)

                                                                                                                                             caacctGTACACCACCAAGGTGGTGAACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCa          >  1:2187291/6‑150 (MQ=255)

                                                                                                                                               ttttGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCctggctgg                       >  1:6403516/1‑135 (MQ=255)

                                                                                                                                               ttttGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCAtt        >  1:7464737/1‑150 (MQ=255)

                                                                                                                                               ttttGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCAtt        >  1:8607590/1‑150 (MQ=255)

                                                                                                                                               ttttGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCAtt        >  2:1549758/1‑150 (MQ=255)

                                                                                                                                                 ctGTACACCACCAAGGTGGTGAACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGc      >  2:5727133/2‑150 (MQ=255)

                                                                                                                                                  tGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCg     <  2:7105645/150‑1 (MQ=255)

                                                                                                                                                  tGTACACCACCAAGGTGGTGAACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCg     >  1:5380440/1‑150 (MQ=255)

GGTGACATGGTTGTTGGTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAA  >  NC_002947/1492856‑1493154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`