BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,554,891	C→T	5.1%	V100V (GTC→GTT)	groL →	chaperonin GroEL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,554,891	0	C	T	5.1%	1192.7 / 30.8	370	V100V (GTC→GTT)	groL	chaperonin GroEL
Reads supporting (aligned to +/- strand): ref base C (156/194); new base T (8/11); total (164/206)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 372 total aligned reads displayed.

AAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCAGGTAGGTACCAT  >  NC_002947/1554742‑1555040

 aGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCa                                                                                                                                                      >  1:398250/1‑150 (MQ=255)

  ggACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCaa                                                                                                                                                     <  1:1170942/150‑1 (MQ=255)

   gACGGCGTGTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGACGTTGCGTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAAc                                                                                                                                                    <  1:5196483/150‑1 (MQ=255)

    aCGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCaa                                                                                                                                                     >  2:5275926/1‑148 (MQ=255)

     cGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGa                                                                                                                                                  >  1:5430762/1‑150 (MQ=255)

      ggCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGACGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGaa                                                                                                                                                 >  2:7319072/1‑150 (MQ=255)

      ggCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGaa                                                                                                                                                 >  2:5609887/1‑150 (MQ=255)

       gCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAg                                                                                                                                                <  1:2799058/150‑1 (MQ=255)

        cGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAgg                                                                                                                                               <  2:3118595/150‑1 (MQ=255)

         gTTTCCGTCGCCACAGAAATCGAGCTGAAAGACGCGTTCGACAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGc                                                                                                                                              <  2:6484298/150‑1 (MQ=255)

         gTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCTTCGTTGACGATGGc                                                                                                                                              <  1:639750/150‑1 (MQ=255)

         gTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGc                                                                                                                                              <  1:2294356/150‑1 (MQ=255)

           ttCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCt                                                                                                                                            <  1:2659619/150‑1 (MQ=255)

           gtCCGTCGCCAAAGAAATCGAGCTGAAAGACGCGTTCGAAAACAGGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCt                                                                                                                                            <  2:8154027/149‑1 (MQ=255)

             ccGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCTTCGTTGACGATGGCCTGa                                                                                                                                          <  2:7658992/150‑1 (MQ=255)

             ccGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCTTCGTTGACGATGGCCTGa                                                                                                                                          <  1:7433098/150‑1 (MQ=255)

             ccGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGa                                                                                                                                          <  1:4857461/150‑1 (MQ=255)

               gTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGaaa                                                                                                                                        <  1:532420/150‑1 (MQ=255)

               gTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGaaa                                                                                                                                        <  1:5660707/150‑1 (MQ=255)

               gTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGaaa                                                                                                                                        <  2:8559673/150‑1 (MQ=255)

                tCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAg                                                                                                                                       <  1:2041434/150‑1 (MQ=255)

                    cAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGTTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGt                                                                                                                                   >  2:4665094/1‑150 (MQ=255)

                    cAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGt                                                                                                                                   <  2:2135293/150‑1 (MQ=255)

                     aaaGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTc                                                                                                                                  >  1:4691616/1‑150 (MQ=255)

                      aagacctCGCGCTGACAGCCGCTTTCGAAACCATGGGCGCCCAGCTGGTCCAGGAAGTTGCGTCCCAGGCCAACGACGCTGCCGGTGACGGCACCACCCCCGCGACCGTTCTTGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCg                                                                                                                                 <  2:3321183/144‑1 (MQ=255)

                       aGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGc                                                                                                                                >  2:1260787/1‑150 (MQ=255)

                          aaTCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGc                                                                                                                             >  2:1110347/1‑150 (MQ=255)

                            tCGAGCTGAAAGACGCTTTCGAAACCATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCg                                                                                                                           <  2:4863967/150‑1 (MQ=255)

                                        aCGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGa                                                                                                               <  1:5890691/150‑1 (MQ=255)

                                            tttCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGa                                                                                                           >  1:4504397/1‑150 (MQ=255)

                                            tttCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGa                                                                                                           >  2:4052050/1‑150 (MQ=255)

                                              tCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGAcc                                                                                                         >  1:4954909/1‑150 (MQ=255)

                                                gAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTg                                                                                                       >  1:777820/1‑150 (MQ=255)

                                                 aaaaCATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGa                                                                                                      >  2:1400005/1‑150 (MQ=255)

                                                  aaaCATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGaa                                                                                                     <  1:5924406/150‑1 (MQ=255)

                                                      aTGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAgcgc                                                                                                 <  2:4446641/150‑1 (MQ=255)

                                                       tGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGcgcg                                                                                                <  2:3768101/150‑1 (MQ=255)

                                                       tGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGcgcg                                                                                                <  1:6181840/150‑1 (MQ=255)

                                                         ggCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGt                                                                                              <  1:7892131/150‑1 (MQ=255)

                                                           cgcCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTAt                                                                                            <  2:104628/150‑1 (MQ=255)

                                                              ccAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGa                                                                                         <  1:6494144/150‑1 (MQ=255)

                                                              ccAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGa                                                                                         >  1:3210695/1‑150 (MQ=255)

                                                              ccAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGa                                                                                         >  1:8395606/1‑150 (MQ=255)

                                                              ccAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGa                                                                                         >  1:1068738/1‑150 (MQ=255)

                                                                 gCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACaa                                                                                      <  1:8093516/150‑1 (MQ=255)

                                                                 gCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACaa                                                                                      >  2:5136005/1‑150 (MQ=255)

                                                                   tGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAgg                                                                                    <  1:183603/150‑1 (MQ=255)

                                                                   tGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAgg                                                                                    <  1:6775499/150‑1 (MQ=255)

                                                                    ggTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGc                                                                                   <  1:2087066/150‑1 (MQ=255)

                                                                     gTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGcc                                                                                  >  2:2724070/1‑150 (MQ=255)

                                                                       cAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCAc                                                                                <  2:4096388/150‑1 (MQ=255)

                                                                       cAAGGAAGTGGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAACGCCAc                                                                                <  1:1682511/150‑1 (MQ=255)

                                                                        aaGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCAct                                                                               >  2:6401386/1‑150 (MQ=255)

                                                                        aaGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCAct                                                                               >  1:6250426/1‑150 (MQ=255)

                                                                         aGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGAGGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCActg                                                                              >  2:7171485/1‑150 (MQ=255)

                                                                         aGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTAAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCActg                                                                              <  2:4565920/150‑1 (MQ=255)

                                                                                   ttCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCCACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCcgtcgt                                                                    >  1:389868/1‑150 (MQ=255)

                                                                                   ttCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCcgtcgt                                                                    >  2:5938572/1‑150 (MQ=255)

                                                                                   ttCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCcgtcgt                                                                    <  2:4397000/150‑1 (MQ=255)

                                                                                   ttCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCcgtcgt                                                                    >  2:1798270/1‑150 (MQ=255)

                                                                                   atCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGAGCGGTATCGACAAGGCCACTGCTGCcgtcgt                                                                    >  2:323019/2‑150 (MQ=255)

                                                                                      cAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGc                                                                 <  1:4206203/150‑1 (MQ=255)

                                                                                       aaGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGcc                                                                >  1:4151718/1‑150 (MQ=255)

                                                                                       aaGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCAGCCGTCGTTGcc                                                                >  1:8623987/1‑150 (MQ=255)

                                                                                          gCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGaa                                                             <  1:891738/150‑1 (MQ=255)

                                                                                          gCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCGGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGaa                                                             <  2:795182/150‑1 (MQ=255)

                                                                                              acgacgCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTga                                                         <  2:5795399/150‑1 (MQ=255)

                                                                                               cgacgCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTgaa                                                        <  1:4595569/150‑1 (MQ=255)

                                                                                                   gCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAAc                                                    >  1:6906531/1‑150 (MQ=255)

                                                                                                     tGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCt                                                  >  2:2847988/1‑150 (MQ=255)

                                                                                                       ccGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGt                                                <  1:2740775/150‑1 (MQ=255)

                                                                                                             aCGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGc                                          >  2:1981219/1‑150 (MQ=255)

                                                                                                               ggCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCa                                        >  2:2995896/1‑150 (MQ=255)

                                                                                                                     accaccGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGcc                                  >  1:7090435/1‑150 (MQ=255)

                                                                                                                      ccaccGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCg                                 >  1:6421968/1‑150 (MQ=255)

                                                                                                                      ccaccGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCg                                 >  1:4414119/1‑150 (MQ=255)

                                                                                                                       caccGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGa                                >  2:6932938/1‑150 (MQ=255)

                                                                                                                       caccGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGa                                >  1:1463548/1‑150 (MQ=255)

                                                                                                                        accGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGAc                               <  1:3556078/150‑1 (MQ=255)

                                                                                                                         ccGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACt                              <  1:1271864/150‑1 (MQ=255)

                                                                                                                             tACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCaa                          <  2:8395606/150‑1 (MQ=255)

                                                                                                                             tACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCaa                          <  2:6151295/150‑1 (MQ=255)

                                                                                                                              aCCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAg                         <  2:3897467/150‑1 (MQ=255)

                                                                                                                                cGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGc                       >  1:380659/1‑150 (MQ=255)

                                                                                                                                       tttCAGGCCTTCGTTGACGATGGCCTGAGAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGcc                >  1:7704929/3‑150 (MQ=255)

                                                                                                                                       tttCAGGCCTTCGTTGACGATGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGcc                >  1:7931691/3‑150 (MQ=255)

                                                                                                                                        cTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGACCCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGccc               <  2:8748626/150‑1 (MQ=255)

                                                                                                                                        cTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGccc               <  2:3541748/150‑1 (MQ=255)

                                                                                                                                        cTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGccc               <  2:1582504/150‑1 (MQ=255)

                                                                                                                                         tCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCa              <  1:2398670/150‑1 (MQ=255)

                                                                                                                                         tCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCa              >  1:2954835/1‑150 (MQ=255)

                                                                                                                                         tCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCa              >  1:8757195/1‑150 (MQ=255)

                                                                                                                                         tCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCa              <  1:7526420/150‑1 (MQ=255)

                                                                                                                                         tCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCAGGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCa              >  1:7044229/1‑150 (MQ=255)

                                                                                                                                          cAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCag             <  2:416609/150‑1 (MQ=255)

                                                                                                                                              ccATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCaggtag         <  1:6662435/150‑1 (MQ=255)

                                                                                                                                               cATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCaggtagg        <  2:4200662/150‑1 (MQ=255)

                                                                                                                                               cATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCaggtagg        <  2:2399262/150‑1 (MQ=255)

                                                                                                                                               cATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCaggtagg        <  2:1882306/150‑1 (MQ=255)

                                                                                                                                                aTCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCaggtaggt       >  1:362242/1‑150 (MQ=255)

AAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCAGGTAGGTACCAT  >  NC_002947/1554742‑1555040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`