BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,554,892	A→G	5.2%	N101D (AAC→GAC)	groL →	chaperonin GroEL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,554,892	0	A	G	5.2%	1171.3 / 30.1	362	N101D (AAC→GAC)	groL	chaperonin GroEL
Reads supporting (aligned to +/- strand): ref base A (154/188); new base G (8/11); total (163/199)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 375 total aligned reads displayed.

AGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCAGGTAGGTACCATC  >  NC_002947/1554743‑1555041

aGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCa                                                                                                                                                       <  1:5962680/150‑1 (MQ=255)

 ggACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCaa                                                                                                                                                      <  1:1170942/150‑1 (MQ=255)

  gACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAAc                                                                                                                                                     >  1:4861773/1‑150 (MQ=255)

  gACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAAc                                                                                                                                                     <  1:4609119/150‑1 (MQ=255)

     ggCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGaa                                                                                                                                                  >  2:5609887/1‑150 (MQ=255)

     ggCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGaa                                                                                                                                                  <  1:2678142/150‑1 (MQ=255)

      gCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAg                                                                                                                                                 <  2:7209364/150‑1 (MQ=255)

      gCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAg                                                                                                                                                 <  1:3744088/150‑1 (MQ=255)

       cGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAgg                                                                                                                                                <  2:8451573/150‑1 (MQ=255)

        gTTTCCGTCGCCAAAGAACTCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCTTCGTTGACGATGGc                                                                                                                                               <  2:2750207/150‑1 (MQ=255)

        gTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGc                                                                                                                                               <  1:7362500/150‑1 (MQ=255)

            ccGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCTTCGTTGACGATGGCCTGa                                                                                                                                           <  1:7468873/150‑1 (MQ=255)

              gTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGaaa                                                                                                                                         <  2:8559673/150‑1 (MQ=255)

              gTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCAAGATGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACAACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGTCCTGaaa                                                                                                                                         >  1:6410797/1‑150 (MQ=255)

              ctCGACAAACAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGaaa                                                                                                                                         <  1:5373528/149‑1 (MQ=255)

                cGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGc                                                                                                                                       <  1:2345402/150‑1 (MQ=255)

                 gCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTTTCAGGCCTTCGTTGACGATGGCCTGagcc                                                                                                                                        <  2:1827688/150‑4 (MQ=255)

                    aaaGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTc                                                                                                                                   >  1:4691616/1‑150 (MQ=255)

                    aaaGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTc                                                                                                                                   <  2:1912144/150‑1 (MQ=255)

                    aaaGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTc                                                                                                                                   >  2:3779582/1‑150 (MQ=255)

                    aaaGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTc                                                                                                                                   <  1:8467581/150‑1 (MQ=255)

                    aaaGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTc                                                                                                                                   <  2:5451415/150‑1 (MQ=255)

                    aaaGAAATAGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGCCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTc                                                                                                                                   >  1:5902784/1‑150 (MQ=255)

                      aGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGc                                                                                                                                 >  2:1260787/1‑150 (MQ=255)

                      aGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGc                                                                                                                                 <  1:755456/150‑1 (MQ=255)

                      aGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGc                                                                                                                                 >  1:6566094/1‑150 (MQ=255)

                                  gtaaGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGaa                                                                                                                     >  2:4179321/3‑150 (MQ=255)

                                   aaaGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAAc                                                                                                                    >  1:2910617/1‑150 (MQ=255)

                                   aaaGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAAc                                                                                                                    <  2:8022184/150‑1 (MQ=255)

                                        cGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGAt                                                                                                               <  1:4997320/150‑1 (MQ=255)

                                        cGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGAt                                                                                                               <  2:304546/150‑1 (MQ=255)

                                         gCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATg                                                                                                              <  2:198653/150‑1 (MQ=255)

                                               gAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTg                                                                                                        >  2:6980992/1‑150 (MQ=255)

                                                 aaaCATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGACCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGaa                                                                                                      >  2:5948349/1‑150 (MQ=255)

                                                  aaCATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAg                                                                                                     >  2:4668198/1‑150 (MQ=255)

                                                     aTGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAgcgc                                                                                                  >  1:3815337/1‑150 (MQ=255)

                                                      tGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGcgcg                                                                                                 >  2:4479680/1‑150 (MQ=255)

                                                       gggCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCgg                                                                                                <  1:8231238/150‑1 (MQ=255)

                                                           gcCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATc                                                                                            <  1:1733064/150‑1 (MQ=255)

                                                             ccAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGa                                                                                          <  1:6451878/150‑1 (MQ=255)

                                                             ccAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGa                                                                                          <  1:4508041/150‑1 (MQ=255)

                                                             ccAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGa                                                                                          >  1:1068738/1‑150 (MQ=255)

                                                             ccAGCTGGTCAAGGAAGTTGCTTCCAACGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGa                                                                                          <  1:2464259/150‑1 (MQ=255)

                                                                gCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACaa                                                                                       <  1:7050736/150‑1 (MQ=255)

                                                                  tGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAgg                                                                                     <  2:4060897/150‑1 (MQ=255)

                                                                  tGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAgg                                                                                     <  1:183603/150‑1 (MQ=255)

                                                                    gTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGcc                                                                                   >  2:3474722/1‑150 (MQ=255)

                                                                     tCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCa                                                                                  <  2:2199400/150‑1 (MQ=255)

                                                                      cAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCAc                                                                                 <  1:417546/150‑1 (MQ=255)

                                                                       aaGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCTATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCAct                                                                                >  1:2769789/1‑150 (MQ=255)

                                                                       aaGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCAct                                                                                >  2:6401386/1‑150 (MQ=255)

                                                                        aGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCActg                                                                               >  1:6215607/1‑150 (MQ=255)

                                                                           aaGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCActgctg                                                                            <  2:6652434/150‑1 (MQ=255)

                                                                               tGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTCCTGCcgt                                                                        >  2:4658306/1‑150 (MQ=255)

                                                                                  ttCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCcgtcgt                                                                     >  1:7822087/1‑150 (MQ=255)

                                                                                  ttCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCcgtcgt                                                                     >  2:7698814/1‑150 (MQ=255)

                                                                                  ttCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTACTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCcgtcgt                                                                     >  2:6177351/1‑150 (MQ=255)

                                                                                     cAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGc                                                                  >  1:5587688/1‑150 (MQ=255)

                                                                                     cAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGc                                                                  >  2:1613379/1‑150 (MQ=255)

                                                                                     cAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGc                                                                  <  1:4206203/150‑1 (MQ=255)

                                                                                        ggCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGa                                                               >  2:1066560/1‑150 (MQ=255)

                                                                                             acgacgCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTga                                                          <  2:1149792/150‑1 (MQ=255)

                                                                                               gacgCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTgaag                                                        <  2:3256048/150‑1 (MQ=255)

                                                                                               gacgCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTgaag                                                        <  1:3017803/150‑1 (MQ=255)

                                                                                                  gCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAAc                                                     <  1:6374410/150‑1 (MQ=255)

                                                                                                     gCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACACGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTg                                                  <  1:3257812/150‑1 (MQ=255)

                                                                                                      ccGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGt                                                 <  1:7933573/150‑1 (MQ=255)

                                                                                                          tGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCaa                                             <  2:2866117/150‑1 (MQ=255)

                                                                                                          tGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCaa                                             <  1:3996534/150‑1 (MQ=255)

                                                                                                           gACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAg                                            <  2:3140080/150‑1 (MQ=255)

                                                                                                              ggCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACGAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCa                                         >  2:4262940/1‑150 (MQ=255)

                                                                                                              ggCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCa                                         >  1:7911842/1‑150 (MQ=255)

                                                                                                              ggCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCa                                         >  2:411956/1‑150 (MQ=255)

                                                                                                                    accaccGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGcc                                   >  1:6933010/1‑150 (MQ=255)

                                                                                                                     ccaccGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCg                                  >  1:2068831/1‑150 (MQ=255)

                                                                                                                      caccGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGa                                 <  1:6861613/150‑1 (MQ=255)

                                                                                                                      caccGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGa                                 >  1:1463548/1‑150 (MQ=255)

                                                                                                                        ccGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACt                               <  1:1271864/150‑1 (MQ=255)

                                                                                                                           cTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCa                            >  2:5799705/1‑150 (MQ=255)

                                                                                                                           cTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCa                            >  1:5270148/1‑150 (MQ=255)

                                                                                                                            tACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCaa                           <  2:8303794/150‑1 (MQ=255)

                                                                                                                             aCCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGTGCCGACTCCAAg                          <  1:5673688/150‑1 (MQ=255)

                                                                                                                             aCCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAg                          <  2:1424345/150‑1 (MQ=255)

                                                                                                                                   cTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATc                    <  1:4456828/150‑1 (MQ=255)

                                                                                                                                    tGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCg                   <  1:3932488/150‑1 (MQ=255)

                                                                                                                                      gctttCAGGCCTTCGTTGACGATGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCg                   >  1:7716645/5‑150 (MQ=255)

                                                                                                                                      gC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATgcgc                                    >  2:4403260/1‑131 (MQ=255)

                                                                                                                                       c‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGACCCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGccc                <  2:8748626/150‑1 (MQ=255)

                                                                                                                                       c‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGccc                <  2:3937399/150‑1 (MQ=255)

                                                                                                                                       c‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGccc                >  1:8652474/1‑150 (MQ=255)

                                                                                                                                          tCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCa               >  1:8757195/1‑150 (MQ=255)

                                                                                                                                            aGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCagg             >  2:7463716/1‑150 (MQ=255)

                                                                                                                                             ggCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCaggt            <  2:519796/150‑1 (MQ=255)

                                                                                                                                               ccATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCaggtag          >  2:3645483/1‑150 (MQ=255)

                                                                                                                                                 aTCGTCAACGAAGGCCTGGAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCaggtaggt        <  1:2138067/150‑1 (MQ=255)

                                                                                                                                                 aTCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCaggtaggt        >  1:359405/1‑150 (MQ=255)

                                                                                                                                                  tCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCAggtaggta       <  2:2581967/150‑1 (MQ=255)

                                                                                                                                                  tCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCAggtaggta       <  1:902140/150‑1 (MQ=255)

                                                                                                                                                      cAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCAGGTAGGTACCAt   >  1:6681297/1‑150 (MQ=255)

                                                                                                                                                       aaCGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCAGGTAGGTACCAtc  >  2:4948899/1‑150 (MQ=255)

AGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGC‑‑TCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGCCGAACTGAAGAACCTGTCCAAGCCATGCGCCGACTCCAAGGCCATCGCCCAGGTAGGTACCATC  >  NC_002947/1554743‑1555041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`