BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,597,595	G→C	7.3%	D67H (GAC→CAC)	PP_1397 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,597,595	0	G	C	7.3%	386.2 / 2.5	147	D67H (GAC→CAC)	PP_1397	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (103/30); new base C (11/2); total (114/33)
Fisher's exact test for biased strand distribution p-value = 7.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 185 total aligned reads displayed.

TCACTCATTTCGAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCATCTTGAACAGGTTGCCCAGCTCGAAATAATCCGCCGGGCCACCCCCACGCAGGATCGGCT  >  NC_002947/1597446‑1597743

  aCTCATTTCGAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCcgac                                                                                                                                                    >  1:6446197/1‑150 (MQ=255)

  aCTCATTTCGAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCcgac                                                                                                                                                    <  1:4396064/150‑1 (MQ=255)

  aCTCATTTCGAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCccac                                                                                                                                                    >  1:6427173/1‑150 (MQ=255)

   cTCATTTCGAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGccgccgccg                                                                                                                                                   >  1:1288471/1‑150 (MQ=255)

   cTCATTTCGAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCcgacg                                                                                                                                                   <  2:3414000/150‑1 (MQ=255)

    tCATTTCGAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCATGGGCGCCGTGGCCGCcgacga                                                                                                                                                  >  2:4985459/1‑150 (MQ=255)

    tCATTTCGAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCcgacga                                                                                                                                                  >  2:5869625/1‑150 (MQ=255)

    tCATTTCGAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCcgacga                                                                                                                                                  >  2:2989217/1‑150 (MQ=255)

    tCATTTCGAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCcgacga                                                                                                                                                  >  1:5237704/1‑150 (MQ=255)

    tCATTTCGAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCcgacga                                                                                                                                                  >  2:2167667/1‑150 (MQ=255)

    tCATTTCGAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCcgacga                                                                                                                                                  >  2:6472475/1‑150 (MQ=255)

    tCATTTCGAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCcgacga                                                                                                                                                  >  2:5637676/1‑150 (MQ=255)

    tCATTTCGAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCCCccacga                                                                                                                                                  >  1:3724572/1‑150 (MQ=255)

     cATTTCGAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAg                                                                                                                                                 >  2:3824244/1‑150 (MQ=255)

     cATTTCGAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAg                                                                                                                                                 <  2:2540163/150‑1 (MQ=255)

     cATTTCGAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAg                                                                                                                                                 <  2:417243/150‑1 (MQ=255)

      aTTTCGAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAgg                                                                                                                                                >  2:2288459/1‑150 (MQ=255)

       tttCGAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCCACCAggc                                                                                                                                               >  1:499565/1‑150 (MQ=255)

        ttCGAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAggcg                                                                                                                                              <  1:7848361/150‑1 (MQ=255)

        ttCGAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAggcg                                                                                                                                              >  1:3454953/1‑150 (MQ=255)

          cGAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAggcggc                                                                                                                                            <  1:3266066/150‑1 (MQ=255)

           gAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGcc                                                                                                                                           >  1:5045598/1‑150 (MQ=255)

           gAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGcc                                                                                                                                           >  1:2806087/1‑150 (MQ=255)

           gAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGcc                                                                                                                                           >  1:6259291/1‑150 (MQ=255)

           gAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGcc                                                                                                                                           >  1:6259735/1‑150 (MQ=255)

           gAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGcc                                                                                                                                           >  1:1024587/1‑150 (MQ=255)

             aGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCAc                                                                                                                                         <  2:8170776/150‑1 (MQ=255)

              gCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCAcc                                                                                                                                        <  2:8091456/150‑1 (MQ=255)

                ggTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCAccgc                                                                                                                                      >  2:1671110/1‑150 (MQ=255)

                  tATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCAccgccg                                                                                                                                    >  2:1396845/1‑150 (MQ=255)

                    tCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAg                                                                                                                                  >  2:3733498/1‑150 (MQ=255)

                    tCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAg                                                                                                                                  >  2:4695662/1‑150 (MQ=255)

                      ggCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCCTCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCCCCGCCGCGCt                                                                                                                                >  2:8528038/1‑150 (MQ=255)

                      ggCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCt                                                                                                                                >  2:2679381/1‑150 (MQ=255)

                      ggCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCt                                                                                                                                >  2:8001979/1‑150 (MQ=255)

                      ggCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCt                                                                                                                                >  1:8553045/1‑150 (MQ=255)

                       gCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTg                                                                                                                               >  2:7496262/1‑150 (MQ=255)

                       gCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCCCCGAGCTg                                                                                                                               >  2:3906231/1‑150 (MQ=255)

                       gCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCCCCCAGCTg                                                                                                                               >  1:5533577/1‑150 (MQ=255)

                                  ggTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGTGCGCCGCGCTTTCACAAGGTTCTCGACGGGCCGGTGTTCAAGGGCGCCGTGGCCGCCGACGCGGCGGCCACCGCCGAGCTGCAACGCCTGCa                                                                                                                    >  2:6206151/1‑150 (MQ=255)

                                  ggTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCAGCa                                                                                                                    >  1:5714192/1‑150 (MQ=255)

                                   gTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCCCCGAGCTGCAACGCcagcag                                                                                                                   >  1:8596184/1‑150 (MQ=255)

                                   gTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCCCCCACGAGGCGGCCCCCCCCCACCAGCAACCCcagcag                                                                                                                   >  1:8432395/1‑150 (MQ=255)

                                      gAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGAGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGc                                                                                                                >  2:1372729/1‑150 (MQ=255)

                                           tGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCCCCCCCGCGGCGGCCCCCCCCCCGCGGCCACGCCggc‑gggcggccgc                                                                                                          >  1:1797449/1‑145 (MQ=255)

                                            ggcggcTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGc                                                                                                          >  2:724299/1‑150 (MQ=255)

                                               ggcTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGaa                                                                                                       >  2:7953778/1‑150 (MQ=255)

                                                  tATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAagag                                                                                                    >  2:3259492/1‑150 (MQ=255)

                                                  tATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGACCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAagag                                                                                                    >  1:3923598/1‑150 (MQ=255)

                                                      aCGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTg                                                                                                >  2:4177292/1‑150 (MQ=255)

                                                      aCGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTg                                                                                                >  2:614103/1‑150 (MQ=255)

                                                       cgcgGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCCCCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGa                                                                                               >  1:3766322/1‑150 (MQ=255)

                                                       cgcgGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGa                                                                                               >  1:7526127/1‑150 (MQ=255)

                                                           gCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCCCCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGctg                                                                                           >  1:4183081/1‑150 (MQ=255)

                                                              aTCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGctggtc                                                                                        >  2:1259027/1‑150 (MQ=255)

                                                              aTCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACCAGGCGGCCACCCCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGctggtc                                                                                        >  1:1334778/1‑150 (MQ=255)

                                                              aTCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCCACCAGGCGGCCAACCCCGAGCAGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGctggtc                                                                                        >  2:3035570/1‑150 (MQ=255)

                                                                     tCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCCCCGAGGCGGCCACCCCCCAGCGGCAACCCCAGCACGGCGGCAGCGAAGAGGGTGGGCGGGGCGGGTGAt                                                                                 >  1:3118799/1‑150 (MQ=255)

                                                                        aaGCCTTGGGCCTGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCCCCCACCACGGGCCCACCCCCGATCAGCAAACCCATCAGGGCCTCAGCCAAGATGGTGAGATGGTCTGGTTATAAg                                                                              >  1:4679222/1‑150 (MQ=255)

                                                                            cTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCcg                                                                          >  1:8012950/1‑150 (MQ=255)

                                                                            cTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCcg                                                                          >  2:6382000/1‑150 (MQ=255)

                                                                            cTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCcg                                                                          >  1:7857334/1‑150 (MQ=255)

                                                                            cTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCCACGAGGCGGCCACCGCCGAGCTGCAACCCCAGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATAAGGCcg                                                                          >  1:2707728/1‑150 (MQ=255)

                                                                             ttGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCcgg                                                                         >  2:7776391/1‑150 (MQ=255)

                                                                             ttGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACC‑CCCAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCcggc                                                                        >  2:7844487/1‑150 (MQ=255)

                                                                                 gCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCCCCCAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCggcggc                                                                     >  2:2334953/1‑150 (MQ=255)

                                                                                  ctgggggggggCGCCGCGCTTACACTTGGTTCTCGAGGGGCTGGTGTTCACGGGCGCGGTGGCCGCCTCGGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGcc                                                                    <  2:7231377/147‑1 (MQ=255)

                                                                                     gggCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCCGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCCCCGAGCTGCAACGCCTGCAGGGCGTCAGCGCAGAGGGTGAGCTGGGCTGGTGATCCGGCCGGCGGCCggg                                                                 >  1:2740005/1‑150 (MQ=255)

                                                                                         ggcgCCCCGGGCTTACACCGGGTTCGCGCGGGGGTGGTTTTCCCGGGGGCCGGGGCCGCCGCCGCGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCAt                                                             <  1:7992897/150‑1 (MQ=255)

                                                                                          gcgcgcCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCATc                                                            >  2:5183964/1‑150 (MQ=255)

                                                                                           ggcgcggcgcTTTCACATGGTTCTCGCGGGGCTGGTGTCCACGGGCGCCGGGGCCGCCGCGGCGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCATCt                                                           <  2:8639372/149‑1 (MQ=255)

                                                                                            gcgccgcgcTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCctctg                                                          >  1:5008490/1‑149 (MQ=255)

                                                                                                 gcgcTTTCACTTGTTGCTCGCGGGGCTGGTGTTCACGGGCGCCGGGGCCGCCGACGCGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCATCTTGAACa                                                     <  2:7942740/150‑1 (MQ=255)

                                                                                                 gcgcTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAAAGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCATCATGAACa                                                     >  2:4619269/1‑150 (MQ=255)

                                                                                                 gcgcTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCCCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCAGGTGATCAGGCCGGCGGCCGGGCCATCTTGAACa                                                     >  2:1166128/1‑150 (MQ=255)

                                                                                                     tttCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGCGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCGGGTCGGGTGATCAGGCCGGCGGCCGGGCCCTCGTGAACAGGtt                                                 >  2:8180864/1‑150 (MQ=255)

                                                                                                        cacaAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCcga                                                                                                                                                     <  1:1171527/47‑1 (MQ=255)

                                                                                                         acaAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGCGGCGGCCACCCCCGAGCGGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCATCTTGAACAGGGTGccc                                             >  2:5438457/1‑150 (MQ=255)

                                                                                                         acaAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCATCTTGAACAGGTTGccc                                             >  2:4773975/1‑150 (MQ=255)

                                                                                                         acaAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCCACGAGGCGGCCCCCCCCCCGCTGCAACCCCTGCCGGGCGTCCGCGAACAGGGGGAGCTGGGCTGGGGATCAGGCCGGCGGCCCGGCCCTCGTGAACAGGTTGccc                                             >  1:2012382/1‑150 (MQ=255)

                                                                                                          cacggTTCCCGCGGGGCTGTTGTTCTGGGGCGCCGGGGCCGCCGAGGCGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCATCTTGAACAGGTTGCCCa                                            <  1:8725509/147‑1 (MQ=255)

                                                                                                           aaGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCATCTTGAACAGGTTGCCCAg                                           >  2:4276282/1‑150 (MQ=255)

                                                                                                           aaGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATAAGGCCGGCGGCCGGGCCATCTTTAACAGGTTGCCCAg                                           >  2:3958036/1‑150 (MQ=255)

                                                                                                           aaGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATAAGGCCGGCGGCCGGGCCATCTTGAAAAGGTTTCCCAg                                           >  2:1479035/1‑150 (MQ=255)

                                                                                                                  tcgcggggCCGGTGTTCCGGGGGGCCGGGCCCGCCGCGGCGGGGCCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCATCTTGAACAGGTTGCCCAGCTCGaaa                                    <  2:2816559/145‑1 (MQ=255)

                                                                                                                     aCGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGGCTGGTGATCAGGCCGGCGGCCGGGCCATCTTGAACAGGTTGCCCAGCTCGAaataa                                 >  1:5045524/1‑150 (MQ=255)

                                                                                                                             gtgtTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCATCTTGAACAGGTTGCCCAGCTCGAAATAATACGCCgg                         >  2:1962325/1‑150 (MQ=255)

                                                                                                                                 tCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCATCTTGAACAGGTTGCCCAGCTCGAAATAATCCGCCGGGCCa                     >  1:7199273/1‑150 (MQ=255)

                                                                                                                                  cAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGTTCAGGCCGGCGGCCGGGCCATCTTGAACAGGTTGCCCAGCTCGAAATAATCCGCCGGGCCAc                    >  2:6984148/1‑150 (MQ=255)

                                                                                                                                    aGGGCGCCGTGGCCGCCGACGAGGCGGCCCCCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCATCTTGAACAGGTTGCCCAGCTCGAAATAATCCGCCGGGCCAccc                  >  2:3367262/1‑150 (MQ=255)

                                                                                                                                          ggggggCCGCCGCCGCGGCGCCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCATCTTGAACAGGTTGCCCAGCTCGAAATAATCCGCCGGGCCACCCCCACGc            <  2:3454953/146‑1 (MQ=255)

                                                                                                                                              ggCCGCCGCCGTGGCGCCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCATCTTGAACAGGTTGCCCAGCTCGAAATAATCCGCCGGGCCACCCCCACGCAGGa        <  2:4353882/150‑1 (MQ=255)

                                                                                                                                               gccgccGACGCGGCGCCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCATCTTGAACAGGTTGCCCAGCTCGAAATAATCCGCCGGGCCACCCCCACGCAGGAt       <  2:8596184/150‑1 (MQ=255)

                                                                                                                                                ccgccgccgTGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCATCTTGAACAGGTTGCCCAGCTCGAAATAATCCGCCGGGCCACCCCCACGCAGGAtc      <  1:5869625/150‑1 (MQ=255)

                                                                                                                                                ccgccgccgCGGCCCCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCATCTTGAACAGGTTGCCCAGCTCGAAATAATCCGCCGGGCCACCCCCACGCAGGAtc      <  1:7492534/150‑1 (MQ=255)

                                                                                                                                                ccgccgCGGGGGCGCCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCATCTTGAACAGGTTGCCCAGCTCGAAATAATCCGCCGGGCCACCCCCACGCAGGAtc      <  2:7116594/150‑1 (MQ=255)

                                                                                                                                                 cgccgACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCATCTTGAACAGGTTGCCCAGCTCGAAATAATCCGCCGGGCCACCCCCACGCAGGAtcg     >  1:829874/1‑150 (MQ=255)

                                                                                                                                                 cgccgACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCATCTTGAACAGGTTGCCCAGCTCGAAATAATCCGCCGGGCCACCCCCACGCAGGAtcg     >  2:1226964/1‑150 (MQ=255)

                                                                                                                                                  gccgAGGGGGCGCCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAGGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCATCTTGAACAGGTTGCCCAGCTCGAAATAATCCGCCGGGCCACCCCCACGCAGGAtcgg    <  2:4927410/150‑1 (MQ=255)

                                                                                                                                                    cgacgaCGCCGCCACCCCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGACGGTGAGCTGGTCTGGAGATCAGGCCGGCGGCCGGGCCCTCTTGAACAGGTTGCCCAGCTCGAAATAATCCGCCCGGCCACCCCCACGCCGGAacggct  >  2:5749079/1‑150 (MQ=255)

TCACTCATTTCGAAGCGGTATCGGCGATGGTGCCGGTGGAAGGTGGCGGCTATCACGCGGCCATCGCGGTCAAAGCCTTGGGCATGGGCGGCGCGCCGCGCTTTCACAAGGTTCTCGACGGGCAGGTGTTCAAGGGCGCCGTGGCCGCCGACGAGGCGGCCACCGCCGAGCTGCAACGCCTGCAGGGCGTCAGCGAAGAGGGTGAGCTGGTCTGGTGATCAGGCCGGCGGCCGGGCCATCTTGAACAGGTTGCCCAGCTCGAAATAATCCGCCGGGCCACCCCCACGCAGGATCGGCT  >  NC_002947/1597446‑1597743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`