BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,709,628	T→A	8.1%	M1* (TTG→TAG) †‡	PP_1499 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,709,628	0	T	A	8.1%	871.9 / 54.8	332	M1* (TTG→TAG) †‡	PP_1499	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (134/151); new base A (17/10); total (152/180)
Fisher's exact test for biased strand distribution p-value = 1.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.22e-01

Only 100 of 345 total aligned reads displayed.

CCGGAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGGACGACAACA  >  NC_002947/1709479‑1709777

ccGGAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCtt                                                                                                                                                       >  2:8236833/1‑150 (MQ=255)

    aggaCAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGccc                                                                                                                                                   <  1:7028684/150‑1 (MQ=255)

     ggaCAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCcg                                                                                                                                                  <  1:1908591/150‑1 (MQ=255)

     ggaCAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCcg                                                                                                                                                  <  2:3605382/150‑1 (MQ=255)

      gaCAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCcgc                                                                                                                                                 <  2:4501991/150‑1 (MQ=255)

            ggcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCGTGCCCGCGAagag                                                                                                                                           >  1:1862103/1‑150 (MQ=255)

             gcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCCTCTTCGCGGGCAAGCCCGCGAagacg                                                                                                                                          <  1:3214038/150‑3 (MQ=255)

                      tGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAg                                                                                                                                 >  2:6290860/1‑150 (MQ=255)

                        cggATCGACAGCCGGGCGGCGCGGGTGCTGGCCTACCTGGCCGACCCGGCCAACTCCGACCTGTTCGTCGACGGGGCGCGGTCCCAGCGCTCGGGCAACTGCCC‑GTGTTGGCGTCTGCGCGGGCTGGCCCGCGAAGAGGCCGGTACAGGt                                                                                                                               <  1:1417244/148‑1 (MQ=255)

                         tGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGtt                                                                                                                              >  2:8388608/1‑150 (MQ=255)

                         tGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGtt                                                                                                                              >  2:5029608/1‑150 (MQ=255)

                           aTCGACAGCCGGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCGGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAACTGACC‑GTGTTGGCGTCTTCGCGGGCTGGCCCGCGAAGAGGCCGGTACAGGTTTc                                                                                                                            <  2:422424/150‑1 (MQ=255)

                              gACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGGTTGCCCGCGAAGAGGCCGGTACAGGTTTGgtg                                                                                                                         >  1:6080279/1‑150 (MQ=255)

                                 accccGGCGGCGCGGTTGCTGGCATACCTGGACCACCCGGCAAAAACCGACCTTTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGACTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTAc                                                                                                                      <  2:5146427/148‑1 (MQ=255)

                                 aGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTAc                                                                                                                      <  2:7433300/150‑1 (MQ=255)

                                   cccGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGAAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTa                                                                                                                    <  2:5982672/150‑1 (MQ=255)

                                    ccGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAg                                                                                                                   >  2:4681958/1‑150 (MQ=255)

                                       gcggcgCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGATGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGaaa                                                                                                                <  1:1276908/150‑1 (MQ=255)

                                       gcggcgCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGaaa                                                                                                                <  2:7317479/150‑1 (MQ=255)

                                        gggcgCGGGTGCTGGCCTACCTGGACGACCCGGCAAACTCCGACCTGTTCGTCGACGTGGCGCGGTTCCAGCGCTCGGGCAAATGCCC‑GTGTTGGCTTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAg                                                                                                               <  1:6815634/149‑1 (MQ=255)

                                        cggcgCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAg                                                                                                               <  1:8674607/150‑1 (MQ=255)

                                        cggcgCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAg                                                                                                               <  1:7281942/150‑1 (MQ=255)

                                         ggcgCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGGTTCGTGTACTAGAAAgg                                                                                                              >  1:3884836/1‑150 (MQ=255)

                                          gcgcGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGATGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTGGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGa                                                                                                             <  2:188657/150‑1 (MQ=255)

                                          gcgcGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGa                                                                                                             <  2:5331075/150‑1 (MQ=255)

                                          gcgcGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGa                                                                                                             <  2:7205100/150‑1 (MQ=255)

                                           cgcgGGTGCTGGCCTACGTGGACGACCCGGCCAACTCCGACCTGTTGGTCGATGGGGCGGGTTTCCAGCGCTGGGGAAAATGACG‑GTGTGGGCGTCTTCGCGGGCTGGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAg                                                                                                            <  1:4577775/150‑1 (MQ=255)

                                               ggTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTcc                                                                                                        <  2:7979956/150‑1 (MQ=255)

                                                  gCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTTCCCGCCAAGAGGCCGGTACAGGTTTAGTGTAGTAGAAAGGAGTTCACgg                                                                                                     >  1:1654621/1‑150 (MQ=255)

                                                   cTGGCCTACCTGGACGACCCGGCAAACTCCGACCTGTTCTTCGAAGTGGGGGGGTTCCAGCGCTGGGGCAAATGACG‑GTTTTGGCGTCTTCGGGGGGTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGt                                                                                                    <  1:8410847/150‑1 (MQ=255)

                                                    tGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGACGAGGCCGGTACAGGTTTCGTGTACGAGAAAGGAGTTCCCGGTg                                                                                                   >  2:8672682/1‑150 (MQ=255)

                                                    tGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTg                                                                                                   >  1:585256/1‑150 (MQ=255)

                                                    gggCCTACCTGGACGCCCCGGCAACCTCCGACCTGTTCGTCGATGGGGGGGGGTTCCCGCGCT‑GGGGAAATGGCCGGTTTTGGCTTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTg                                                                                                   <  1:6689075/149‑1 (MQ=255)

                                                    gggCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTg                                                                                                   <  2:2855614/149‑1 (MQ=255)

                                                        cTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACg                                                                                               >  1:3557827/1‑150 (MQ=255)

                                                           ccgggccgacCCGCCAAACCCCGCCCGGTTCGACGACGGGGCGCGTTCCCCGCGCTGGGGAAAATGCCG‑GTTTTGGGGTCTGCGCGGGCTTCCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAt                                                                                            <  1:3765756/144‑1 (MQ=255)

                                                           ccTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTATAAAGGAGTTACCGGTGGACGAAt                                                                                            >  1:7918848/1‑150 (MQ=255)

                                                               gacgacCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCa                                                                                        <  2:6994086/150‑1 (MQ=255)

                                                               gacgacCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCa                                                                                        <  2:2903085/150‑1 (MQ=255)

                                                               gacgacCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCa                                                                                        <  2:5637899/150‑1 (MQ=255)

                                                               gacgacCCGGCCAACCCCGACGTGTTGGTCGACGGGGGGGGGTTCCCGCGCTGGGGCAATTGACG‑GTGTGGGCGTCTTCGGGGGCTGGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCa                                                                                        <  1:429990/150‑1 (MQ=255)

                                                                  gacCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCa                                                                                     <  2:770173/150‑1 (MQ=255)

                                                                     ccGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAAc                                                                                  >  1:4363498/1‑150 (MQ=255)

                                                                     ccGGCCAACACCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGAGCTCGGTCAAATGACC‑GTGTACGCGACATCGCGGGCTTGACCGCGAAGAGGCCGGTAAAGGTATCTAGTACTAGAAAGGAGTACCACGTGGACCAATACCAGCAAAc                                                                                  >  1:8016842/1‑150 (MQ=255)

                                                                        gCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTg                                                                                                   >  2:7491070/1‑130 (MQ=255)

                                                                          cAACTCCGACCTGTTCGTCGAAGTGGCGGGGTGCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTGGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATcc                                                                             <  1:6268015/150‑1 (MQ=255)

                                                                            aCTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCcgc                                                                           <  1:5757193/150‑1 (MQ=255)

                                                                            aCTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCcgc                                                                           >  1:3474710/1‑150 (MQ=255)

                                                                              tCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCCGTACAGGTTTAGTGTGATAGAAAAGAGTTACCCGTGGACGAATACCAGCAAACCATCCgcgc                                                                         >  1:473720/1‑150 (MQ=255)

                                                                                cGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCGTGCCCCCCAAGAGGCCCGGACAGGTTTGGGGTGAGAGAAAGGCGTTGCCCGTGGGCGAATAACACCAAACCAACCCCGccc                                                                       >  1:1182111/1‑150 (MQ=255)

                                                                                   ccTGTTCGTCGACGTGGCGCGGTTCCAGCGCTCGGGCAATTGACC‑GTGTTGGCTTCTTCGGGGGCTGGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGt                                                                    <  1:4104082/150‑1 (MQ=255)

                                                                                   ccTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGGCC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGt                                                                    <  2:3476162/150‑1 (MQ=255)

                                                                                      gTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCCCCCTGTCCg                                                                 >  2:5492162/1‑150 (MQ=255)

                                                                                          gacgAAGTGGCGAGGTTCCAGCGCTCGGGCAAATGACC‑GTGAAGGCGACATAGCCGGAATCCCCGCGAAGATGCCGGTACAGGTTTCGAGAAAAACAAAGGACTTCCCGGTGGAAGAATACCACCAAACCAACCGCGCCCACTCCGACCg                                                             >  2:5884466/3‑150 (MQ=255)

                                                                                                   gcgcGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTcgc                                                    <  1:5246139/150‑1 (MQ=255)

                                                                                                   gcgcGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTcgc                                                    <  1:7168607/150‑1 (MQ=255)

                                                                                                    cgcgGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTcgcg                                                   <  1:5001439/150‑1 (MQ=255)

                                                                                                    cgcgGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTcgcg                                                   <  2:3736698/150‑1 (MQ=255)

                                                                                                     gcgGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTAAAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCCGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCgg                                                  >  2:6187384/1‑150 (MQ=255)

                                                                                                     gcgGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCCGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCgg                                                  >  2:331902/1‑150 (MQ=255)

                                                                                                        gTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGcac                                               <  2:5775565/150‑1 (MQ=255)

                                                                                                         ttCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGcaca                                              <  1:2791048/150‑1 (MQ=255)

                                                                                                         ttCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGcaca                                              <  2:2352592/150‑1 (MQ=255)

                                                                                                           ccAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGa                                            >  2:5699880/1‑150 (MQ=255)

                                                                                                            cAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGAc                                           >  2:8054812/1‑150 (MQ=255)

                                                                                                             aGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACg                                          >  2:899438/1‑150 (MQ=255)

                                                                                                             aGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCGTGCCCGCGAAGAGGCCGGGACAGGTTTCGTGTACTAGCAAGGAGTTCCCGGTGGACGAATACCAGCCAAACATCCGCGCCCGGTCCGCCCGCCTCGTCGCGGCACAGACg                                          >  2:7645350/1‑150 (MQ=255)

                                                                                                               cgcTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGcc                                        <  1:2649517/150‑1 (MQ=255)

                                                                                                               aacacGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCCCCAAAAGGCCGGTACAGGTTTCGTGTAAAATAAATGAGTTCCCGGTGGCAGAATAAAACCAAAACAAACGCCCCCAGTACGACCCCATCCTCGCGGCACAAACGcc                                        >  1:6489268/5‑150 (MQ=255)

                                                                                                                 cTCGGGCATATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGa                                      <  2:2614063/150‑1 (MQ=255)

                                                                                                                      gCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCgg                                 <  1:5953426/150‑1 (MQ=255)

                                                                                                                          aTGACC‑GTGTTGGCGTCTTCGCGGGCTGGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCCAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTcc                             <  1:932776/150‑1 (MQ=255)

                                                                                                                           tGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCt                            <  1:8236833/150‑1 (MQ=255)

                                                                                                                           tGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCt                            <  2:2566037/150‑1 (MQ=255)

                                                                                                                           tGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCt                            <  1:2248727/150‑1 (MQ=255)

                                                                                                                              cc‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAATGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGGCGCGGCACAGACGCCGATCCGGGTCCATGa                         >  2:6437980/1‑150 (MQ=255)

                                                                                                                              cc‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGa                         <  2:2791415/150‑1 (MQ=255)

                                                                                                                                   gtTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGAcgcg                     <  1:5466398/150‑1 (MQ=255)

                                                                                                                                     tGGCGTCTTGGCGGGGTTGGCCGCGAAGAGGCCGGTACAGGTTTCGTGTTCTAGAAAGGAGTTGCCGGTGGTCGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTGGCGGCACAGACGCCGATCCGGGTGCTTGACGCGGt                   >  2:1506024/1‑150 (MQ=255)

                                                                                                                                      ggCGTCTTCGCGGGCGTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTg                  <  1:753974/150‑1 (MQ=255)

                                                                                                                                        cGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGaa                <  2:3014397/150‑1 (MQ=255)

                                                                                                                                        cGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGaa                <  1:5562309/150‑1 (MQ=255)

                                                                                                                                          tCTTCGCGGGCAAGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGt              >  1:2459238/1‑150 (MQ=255)

                                                                                                                                          tCTTCGCGGGCAAGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGt              >  2:6629772/1‑150 (MQ=255)

                                                                                                                                          tCTTCGCGGGCAAGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCAGGGTCCTTGACGCGGTGAAGt              >  1:3377847/1‑150 (MQ=255)

                                                                                                                                           cTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTg             <  2:4100411/150‑1 (MQ=255)

                                                                                                                                            ttCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCGAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTgg            >  1:7266288/1‑150 (MQ=255)

                                                                                                                                            ttCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTgg            >  2:2054325/1‑150 (MQ=255)

                                                                                                                                            ttCGCGGGCAAGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTgg            >  1:6938283/1‑150 (MQ=255)

                                                                                                                                            ttCGCGGGCAAGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTgg            >  1:634462/1‑150 (MQ=255)

                                                                                                                                             tCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTggg           >  2:671514/1‑150 (MQ=255)

                                                                                                                                               gcgGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGgac         >  2:128260/1‑150 (MQ=255)

                                                                                                                                               gcgGGCTGGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGgac         <  1:7735652/150‑1 (MQ=255)

                                                                                                                                                cgGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGgacg        <  1:6171014/150‑1 (MQ=255)

                                                                                                                                                cgGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGgacg        <  1:1747583/150‑1 (MQ=255)

                                                                                                                                                 gggCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGgacga       >  2:6813464/1‑150 (MQ=255)

                                                                                                                                                     ttGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGGACGacaac   >  1:5491833/1‑150 (MQ=255)

                                                                                                                                                     ttGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGGACGacaac   >  1:4322294/1‑150 (MQ=255)

                                                                                                                                                     ttGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGGACGacaac   >  1:5339317/1‑150 (MQ=255)

                                                                                                                                                     ttGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGGACGacaac   <  1:4901357/150‑1 (MQ=255)

CCGGAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACC‑GTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGGACGACAACA  >  NC_002947/1709479‑1709777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`