BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,770,235	G→T	5.2%	G137C (GGC→TGC)	PP_1574 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,770,235	0	G	T	5.2%	1200.8 / 35.9	354	G137C (GGC→TGC)	PP_1574	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (193/142); new base T (10/9); total (203/151)
Fisher's exact test for biased strand distribution p-value = 8.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 361 total aligned reads displayed.

CGCTTGACCCGGTTGAACTGGAGAAGGACCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGTGGAGCGCGCCACGGGC  >  NC_002947/1770086‑1770383

cGCTTGACCCGGTTGAACTGGAGAAGGACCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTg                                                                                                                                                      <  1:5294493/150‑1 (MQ=255)

cGCTTGACCCGGTTGAACTGGAGAAGGACCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTg                                                                                                                                                      >  1:6467114/1‑150 (MQ=255)

 gCTTGACCCGGTTGAACTGGAGAAGGACCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTgg                                                                                                                                                     <  1:837112/150‑1 (MQ=255)

   ttGACCCGGTTGAACTGGAGAAGGACCCGGAGAGTACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCa                                                                                                                                                   >  2:7112450/1‑150 (MQ=255)

       cccGGTTGAACTGGAGAAGGACCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAg                                                                                                                                               >  2:1855323/1‑150 (MQ=255)

        ccGGTTGAACTGGAGAAGGACCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGc                                                                                                                                              <  1:4932953/150‑1 (MQ=255)

        ccGGTTGAACTGGAGAAGGACCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGc                                                                                                                                              >  1:4769494/1‑150 (MQ=255)

          ggTTGAACTGGAGAAGGACCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTc                                                                                                                                            >  2:597794/1‑150 (MQ=255)

          ggTTGAACTGGAGAAGGACCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTc                                                                                                                                            >  2:3691989/1‑150 (MQ=255)

             tGAACTGGAGAAGGACCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCGTGCCAGAGCTCGTc                                                                                                                                         <  2:2453327/150‑1 (MQ=255)

              gAACTGGAGAAGGACCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTcc                                                                                                                                        <  1:7351225/150‑1 (MQ=255)

               aaCTGGAGAAGGACCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCGTGCCAGAGCTCGTcga                                                                                                                                       <  1:7662101/150‑3 (MQ=255)

                  tGGAGAAGGACCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCAGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAATACGAAGAGATCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCtctc                                                                                                                                    >  1:2888896/1‑150 (MQ=255)

                    gagaAGGACCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGGCCTCTCCg                                                                                                                                  >  2:8049291/1‑150 (MQ=255)

                           aCCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATcgg                                                                                                                           <  2:4292420/150‑1 (MQ=255)

                               ggAGAGCACCACGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCggcggc                                                                                                                       <  2:8339927/150‑1 (MQ=255)

                               ggAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCggcggc                                                                                                                       <  1:5505183/150‑1 (MQ=255)

                               ggAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCggcggc                                                                                                                       >  2:5867674/1‑150 (MQ=255)

                                   agCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTcga                                                                                                                   <  2:8512018/150‑1 (MQ=255)

                                    gCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTcgac                                                                                                                  <  2:7555813/150‑1 (MQ=255)

                                      aCCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTcgacga                                                                                                                >  1:2789666/1‑150 (MQ=255)

                                      aCCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTcgacga                                                                                                                >  1:864781/1‑150 (MQ=255)

                                      aCCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTcgacga                                                                                                                <  1:5377011/150‑1 (MQ=255)

                                       ccAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGAt                                                                                                               <  2:3096235/150‑1 (MQ=255)

                                       ccAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGAt                                                                                                               >  1:1769360/1‑150 (MQ=255)

                                             gcCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCa                                                                                                         <  1:7128683/150‑1 (MQ=255)

                                               cTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAgg                                                                                                       <  2:8137916/150‑1 (MQ=255)

                                                   gATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCaa                                                                                                   >  2:2594694/1‑150 (MQ=255)

                                                      ctctTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGCAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGa                                                                                                <  2:2818606/150‑1 (MQ=255)

                                                       tctTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGaa                                                                                               >  2:2915373/1‑150 (MQ=255)

                                                       tctTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGaa                                                                                               >  1:5174699/1‑150 (MQ=255)

                                                       tctTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGaa                                                                                               >  1:3041839/1‑150 (MQ=255)

                                                          tGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAAc                                                                                            >  1:7285939/1‑150 (MQ=255)

                                                           gATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAAcc                                                                                           >  2:7400572/1‑150 (MQ=255)

                                                            aTGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCt                                                                                          >  1:3683726/1‑150 (MQ=255)

                                                              ggCGACCTGTCCGTCGCGCTGCTGGTAGCTGTTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGa                                                                                        <  1:6908934/150‑1 (MQ=255)

                                                              ggCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGa                                                                                        <  1:4613154/150‑1 (MQ=255)

                                                                cGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGc                                                                                      >  2:1481388/1‑150 (MQ=255)

                                                                 gACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGcc                                                                                     >  2:2720055/1‑150 (MQ=255)

                                                                    cTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGTAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGc                                                                                  <  1:2651649/150‑1 (MQ=255)

                                                                    cTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGc                                                                                  >  2:3642288/1‑150 (MQ=255)

                                                                    cTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGc                                                                                  >  2:7844786/1‑150 (MQ=255)

                                                                      gTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGt                                                                                <  1:5711166/150‑1 (MQ=255)

                                                                       tCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTg                                                                               >  1:3395974/1‑150 (MQ=255)

                                                                       tCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTg                                                                               >  1:6667070/1‑150 (MQ=255)

                                                                        gcGTCGGGCTGCTGGTTGCTATTCGGGATGTCAACGACCTGGGCAAGACGAAGAGCTCACCGTGCTCGTCGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGa                                                                              <  1:6245733/149‑1 (MQ=255)

                                                                        ccGTCGCGCTGCTGGTAGGTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGa                                                                              <  1:7013224/150‑1 (MQ=255)

                                                                           tCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGtt                                                                           >  1:1950584/1‑150 (MQ=255)

                                                                            cgcgCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTc                                                                          <  1:791123/150‑1 (MQ=255)

                                                                             gcgcTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCa                                                                         >  1:6093497/1‑150 (MQ=255)

                                                                             gcgcTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCa                                                                         <  2:4753523/150‑1 (MQ=255)

                                                                             gcgcTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCa                                                                         >  1:2364489/1‑150 (MQ=255)

                                                                              cgcTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAg                                                                        <  2:2088402/150‑1 (MQ=255)

                                                                               gctgctGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAgg                                                                       <  1:2453237/150‑1 (MQ=255)

                                                                                 tgctgGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTc                                                                     >  2:8405599/1‑150 (MQ=255)

                                                                                 tgctgGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTc                                                                     >  2:3914312/1‑150 (MQ=255)

                                                                                  gctgGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCt                                                                    >  1:811170/1‑150 (MQ=255)

                                                                                     ggTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTgg                                                                 <  2:2163756/150‑1 (MQ=255)

                                                                                     ggTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTgg                                                                 <  1:6757445/150‑1 (MQ=255)

                                                                                          cTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGaa                                                            <  1:1848782/150‑1 (MQ=255)

                                                                                          cTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGaa                                                            <  1:2210143/150‑1 (MQ=255)

                                                                                             ttCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTa                                                         >  1:103777/1‑150 (MQ=255)

                                                                                               cGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACa                                                       <  2:5567942/150‑1 (MQ=255)

                                                                                               cGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCGGCGTGAGTTCAGGTCTTGGGAGAAGTACa                                                       <  2:2260590/150‑1 (MQ=255)

                                                                                                gggAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAg                                                      >  2:6941096/1‑150 (MQ=255)

                                                                                                gggAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAg                                                      <  2:4075899/150‑1 (MQ=255)

                                                                                                 ggAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAgg                                                     <  1:268723/150‑1 (MQ=255)

                                                                                                  gAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGc                                                    >  1:2313962/1‑150 (MQ=255)

                                                                                                        aaCGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTcc                                              >  1:4335821/1‑150 (MQ=255)

                                                                                                          cGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGt                                            <  1:3628473/150‑1 (MQ=255)

                                                                                                          cGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGt                                            <  2:5482313/150‑1 (MQ=255)

                                                                                                           gACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTg                                           >  1:4426887/1‑150 (MQ=255)

                                                                                                              cTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCt                                        >  1:1672261/1‑150 (MQ=255)

                                                                                                                 ggAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGc                                     <  1:8475347/150‑1 (MQ=255)

                                                                                                                     aGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCa                                 >  2:3839208/1‑150 (MQ=255)

                                                                                                                      gACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCAt                                <  1:1332449/150‑1 (MQ=255)

                                                                                                                       aCGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATg                               >  2:6593515/1‑150 (MQ=255)

                                                                                                                       aCGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATg                               >  2:5120824/1‑150 (MQ=255)

                                                                                                                         gAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTggg                                                                >  2:5726382/1‑115 (MQ=255)

                                                                                                                         gAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTc                             >  2:8079377/1‑150 (MQ=255)

                                                                                                                         gAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTc                             >  2:1292652/1‑150 (MQ=255)

                                                                                                                          aaGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCg                            <  1:3313927/150‑1 (MQ=255)

                                                                                                                            gagCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCCGGCCAAAGAAAACCTGAGCCTGCGTGAGTCCAGGTCTGGGGAGAAGTACAGGCAGCACCGAGGCTCGCTGCATGTCggg                          >  2:6187128/1‑150 (MQ=255)

                                                                                                                                tCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATgc                      <  2:3293790/150‑1 (MQ=255)

                                                                                                                                tCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATgc                      >  1:3929195/1‑150 (MQ=255)

                                                                                                                                     gAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCCTCGGCGGCTCGACGATCGCGCCGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGAGGCGCGTg                 <  2:864747/150‑1 (MQ=255)

                                                                                                                                     gAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGTg                 >  2:5289212/1‑150 (MQ=255)

                                                                                                                                     gAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGTg                 <  2:6467114/150‑1 (MQ=255)

                                                                                                                                       gCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGTGGa               <  1:8285859/150‑1 (MQ=255)

                                                                                                                                       gCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGTGGa               <  1:6144515/150‑1 (MQ=255)

                                                                                                                                        cTCGACGAGCTCTTGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCAAAATAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCTTGGCTCGCTGCATGTCGGGATGCGCGTGGat              <  2:6745078/150‑2 (MQ=255)

                                                                                                                                          cgacgaGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGTGGAgcg            <  1:5529501/150‑1 (MQ=255)

                                                                                                                                           gacgaGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCCAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGTGGAgcgc           >  2:1142297/1‑150 (MQ=255)

                                                                                                                                           gacgaGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGTGGAgcgc           <  2:1789896/150‑1 (MQ=255)

                                                                                                                                           gacgaGCTCGTGCCAGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCAGGTCGGGATGCGCGTGGAgcgc           >  2:8739882/1‑150 (MQ=255)

                                                                                                                                             cgaGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGTGGAgcgcgc         <  2:2999852/150‑1 (MQ=255)

                                                                                                                                             cgaGCTCGTGCCAGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGTGGAgcgcgc         >  2:8295054/1‑150 (MQ=255)

                                                                                                                                                gCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGTGGAGCGCGCCAc      >  2:4648219/1‑150 (MQ=255)

                                                                                                                                                gCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCGCGACGATCGCGCCGGCCAAAGAAAACCTGCGCCTGCGGGAGTTCAGGTCGTGGGAGCAGTACCGGCAGCTCCGGGGCGCGCTGCCTGGCGGGGGGCGCGTGGAGCGCGCCAc      >  2:6394922/1‑150 (MQ=255)

                                                                                                                                                 ctctGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGTGGAGCGCGCCACg     >  1:5773097/1‑150 (MQ=255)

CGCTTGACCCGGTTGAACTGGAGAAGGACCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGTGGAGCGCGCCACGGGC  >  NC_002947/1770086‑1770383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`