BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,131,470	T→A	5.0%	L617M (TTG→ATG) ‡	PP_1887 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,131,470	0	T	A	5.0%	1257.3 / 32.9	368	L617M (TTG→ATG) ‡	PP_1887	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (190/159); new base A (2/17); total (192/176)
Fisher's exact test for biased strand distribution p-value = 1.87e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 376 total aligned reads displayed.

ACGATGAGCGTGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGCACACACTGCTCGAC  >  NC_002947/2131321‑2131619

    tGAGCGTGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCg                                                                                                                                                   >  1:7616844/1‑150 (MQ=255)

     gAGCGTGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGt                                                                                                                                                  >  2:8361369/1‑150 (MQ=255)

       gCGTGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCg                                                                                                                                                >  2:192768/1‑150 (MQ=255)

       gCGTGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCAAGGCGTCg                                                                                                                                                <  1:2554028/150‑1 (MQ=255)

        cGTGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCgg                                                                                                                                               <  2:1583254/150‑1 (MQ=255)

        cGTGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCAAGGCGTCgt                                                                                                                                               <  2:2708371/150‑2 (MQ=255)

         gTGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCGCCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCAAGGCGTCgta                                                                                                                                              <  1:4623730/150‑3 (MQ=255)

         gTGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCAAGGCGTCgta                                                                                                                                              <  2:1284774/150‑3 (MQ=255)

         gTGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCAAGGCGTCgta                                                                                                                                              <  1:864123/150‑3 (MQ=255)

         gTGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCAAGGCGTCgta                                                                                                                                              <  1:7695694/150‑3 (MQ=255)

         gTGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCAAGGCGTCgta                                                                                                                                              <  1:50776/150‑3 (MQ=255)

          tGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGt                                                                                                                                             <  1:6319040/150‑1 (MQ=255)

          tGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCAAGGCGTCgtag                                                                                                                                             <  1:1095668/150‑4 (MQ=255)

          tGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCAAGGCGTCgtag                                                                                                                                             <  1:5289450/150‑4 (MQ=255)

            cACTGCAGTCGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCAAGGCGTCgta                                                                                                                                              <  1:5670206/147‑3 (MQ=255)

                 cAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACAcgc                                                                                                                                      >  2:7633093/1‑150 (MQ=255)

                      ggTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGt                                                                                                                                 >  2:8228508/1‑150 (MQ=255)

                        tGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCg                                                                                                                               >  2:2148527/1‑150 (MQ=255)

                        tGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCg                                                                                                                               <  1:2465448/150‑1 (MQ=255)

                         ggAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGt                                                                                                                              >  2:5222495/1‑150 (MQ=255)

                          gAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTg                                                                                                                             <  1:6644277/150‑1 (MQ=255)

                          gAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTg                                                                                                                             >  1:1559410/1‑150 (MQ=255)

                           aaaaGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTgg                                                                                                                            <  2:6914645/150‑1 (MQ=255)

                                 tggtggGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAg                                                                                                                      <  1:6241725/150‑1 (MQ=255)

                                  ggtgggtgTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAgg                                                                                                                     >  2:4477133/1‑150 (MQ=255)

                                   gtgggtgTTGACGGTGAGTCCAAGACCATCACCTTTCGACATGCACTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTAGAAATCCTCTTCTACTACGACGCCTTGCGTCGGGTATCACGAGAGGTCGTGGCTCCAGGc                                                                                                                    <  2:5918966/150‑1 (MQ=255)

                                   gtgggtgTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTgg                                                                                                                            <  1:6985760/142‑1 (MQ=255)

                                       gtgtTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGc                                                                                                                <  1:3924089/150‑1 (MQ=255)

                                          ttGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCtt                                                                                                             <  2:7714883/150‑1 (MQ=255)

                                            gACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTc                                                                                                           >  2:4876681/1‑150 (MQ=255)

                                             aCGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCACGCCTTTCg                                                                                                          >  2:6454824/1‑150 (MQ=255)

                                                 tGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGc                                                                                                      <  1:2031305/150‑1 (MQ=255)

                                                 tGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGc                                                                                                      <  1:1831599/150‑1 (MQ=255)

                                                    gcaaagcaCCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGa                                                                                                   >  2:2904587/8‑150 (MQ=255)

                                                     tCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGaa                                                                                                  >  2:3050590/1‑150 (MQ=255)

                                                      ccAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAAc                                                                                                 >  1:3822859/1‑150 (MQ=255)

                                                       cAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACg                                                                                                >  1:6412000/1‑150 (MQ=255)

                                                       cAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACg                                                                                                >  1:4921194/1‑150 (MQ=255)

                                                        aaGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGc                                                                                               >  2:6010612/1‑150 (MQ=255)

                                                        aaGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGc                                                                                               >  1:7682934/1‑150 (MQ=255)

                                                        aaGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGc                                                                                               >  1:5750019/1‑150 (MQ=255)

                                                          gACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCaa                                                                                             <  1:7907798/150‑1 (MQ=255)

                                                             caccACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTa                                                                                          >  1:3976803/4‑150 (MQ=255)

                                                             cATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAAttc                                                                                          >  2:7103226/1‑149 (MQ=255)

                                                             cATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTa                                                                                          >  2:3441232/1‑150 (MQ=255)

                                                                cACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACaa                                                                                       >  2:6569003/1‑150 (MQ=255)

                                                                cACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACaa                                                                                       <  1:4751376/150‑1 (MQ=255)

                                                                 aCCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAg                                                                                      <  2:611925/150‑1 (MQ=255)

                                                                  ccTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGt                                                                                     >  2:2252573/1‑150 (MQ=255)

                                                                   cTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTa                                                                                    >  1:1008431/1‑150 (MQ=255)

                                                                          cATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCctc                                                                             <  2:4682101/150‑1 (MQ=255)

                                                                          cATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCctc                                                                             <  2:6243021/150‑1 (MQ=255)

                                                                           aTGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCctct                                                                            <  2:3959081/150‑1 (MQ=255)

                                                                           aTGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCctct                                                                            >  2:7834799/1‑150 (MQ=255)

                                                                              cccTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAg                                                                                                       >  1:2891340/1‑120 (MQ=255)

                                                                                cTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCa                                                                       >  2:4528406/1‑150 (MQ=255)

                                                                                 tGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCAt                                                                      >  1:6558430/1‑150 (MQ=255)

                                                                                     cacGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGa                                                                  <  2:7902755/150‑1 (MQ=255)

                                                                                        gggCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACaa                                                               >  1:5528165/1‑150 (MQ=255)

                                                                                        gggCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACaa                                                               >  1:8409351/1‑150 (MQ=255)

                                                                                        gggCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACaa                                                               >  1:59805/1‑150 (MQ=255)

                                                                                        gggCGAGCCGCTGCTCAACCTGGACGACAACGATGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACaa                                                               >  2:6037307/1‑150 (MQ=255)

                                                                                         ggCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAAc                                                              >  2:5306433/1‑150 (MQ=255)

                                                                                             aGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGc                                                          <  1:3199113/150‑1 (MQ=255)

                                                                                                 gctgctCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGc                                                      >  2:3335096/1‑150 (MQ=255)

                                                                                                  ctgctCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGcc                                                     >  1:887016/1‑150 (MQ=255)

                                                                                                         aCCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAAt                                              >  2:5638230/1‑150 (MQ=255)

                                                                                                          ccTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATg                                             >  1:7293807/1‑150 (MQ=255)

                                                                                                          ccTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATg                                             <  2:5386039/150‑1 (MQ=255)

                                                                                                          ccTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATg                                             <  2:2493153/150‑1 (MQ=255)

                                                                                                           cTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACGTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATgg                                            <  2:8014967/150‑1 (MQ=255)

                                                                                                             ggACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGc                                          <  2:6984334/150‑1 (MQ=255)

                                                                                                              gacgacAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCACGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGcc                                         >  2:8586041/1‑150 (MQ=255)

                                                                                                              gacgacAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGcc                                         >  2:2340768/1‑150 (MQ=255)

                                                                                                               acgacAACGGTGTCGAAATCCGCTACTAGTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCt                                        <  1:7145160/150‑1 (MQ=255)

                                                                                                                 gacAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTAt                                      >  2:8330607/1‑150 (MQ=255)

                                                                                                                   cAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGa                                    >  2:1210349/1‑150 (MQ=255)

                                                                                                                   cAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGa                                    <  2:3502961/150‑1 (MQ=255)

                                                                                                                    aaCGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGAc                                   >  1:4427681/1‑150 (MQ=255)

                                                                                                                       ggTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTg                                >  1:3620321/1‑150 (MQ=255)

                                                                                                                        gtgtCGAAATCCGCTACTACTACGTCGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGa                               <  2:3211958/150‑1 (MQ=255)

                                                                                                                        gtgtCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGa                               <  1:6771380/150‑1 (MQ=255)

                                                                                                                         tgtCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGaa                              <  1:5177883/150‑1 (MQ=255)

                                                                                                                             gAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCaa                          >  2:2843010/1‑150 (MQ=255)

                                                                                                                              aaaTCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAg                         <  2:6338048/150‑1 (MQ=255)

                                                                                                                              aaaTCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAg                         >  1:3356953/1‑150 (MQ=255)

                                                                                                                               aaTCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACGTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGt                        <  1:8179319/150‑1 (MQ=255)

                                                                                                                                  ccGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCga                     <  1:7496576/150‑1 (MQ=255)

                                                                                                                                   cGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCgag                    <  2:5630072/150‑1 (MQ=255)

                                                                                                                                         tactacGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGcac              >  2:4934440/1‑150 (MQ=255)

                                                                                                                                         tactacGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGcac              >  2:7538148/1‑150 (MQ=255)

                                                                                                                                           ctacGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGcacac            >  2:4796170/1‑150 (MQ=255)

                                                                                                                                            tacGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGcacaca           >  2:135498/1‑150 (MQ=255)

                                                                                                                                              cgacgCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGCACACACt         >  1:8332313/1‑150 (MQ=255)

                                                                                                                                              cgacgCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGCACACACt         >  2:7276301/1‑150 (MQ=255)

                                                                                                                                              cgacgCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGCACACACt         >  2:1769539/1‑150 (MQ=255)

                                                                                                                                                   ccTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGCACACACTGCTCg    <  2:1181825/150‑1 (MQ=255)

                                                                                                                                                     ttGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGCACACACTGCTCGAc  <  2:5772295/150‑1 (MQ=255)

                                                                                                                                                     ttGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGCACACACTGCTCGAc  >  2:3673301/1‑150 (MQ=255)

                                                                                                                                                     ttGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGCACACACTGCTCGAc  <  1:3028416/150‑1 (MQ=255)

ACGATGAGCGTGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGCACACACTGCTCGAC  >  NC_002947/2131321‑2131619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`