BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,131,471	T→G	5.0%	L617W (TTG→TGG) ‡	PP_1887 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,131,471	0	T	G	5.0%	1268.9 / 36.7	368	L617W (TTG→TGG) ‡	PP_1887	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (190/159); new base G (2/17); total (192/176)
Fisher's exact test for biased strand distribution p-value = 1.87e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 381 total aligned reads displayed.

CGATGAGCGTGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGCACACACTGCTCGACG  >  NC_002947/2131322‑2131620

cGATGAGCGTGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCtt                                                                                                                                                       <  2:3625695/150‑1 (MQ=255)

   tGAGCGTGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCg                                                                                                                                                    >  1:7616844/1‑150 (MQ=255)

    gAGCGTGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGt                                                                                                                                                   >  2:8361369/1‑150 (MQ=255)

     aGCGTGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTc                                                                                                                                                  >  2:1846269/1‑150 (MQ=255)

      gCGTGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCAAGGCGTCg                                                                                                                                                 <  1:2554028/150‑1 (MQ=255)

        gTGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCggg                                                                                                                                               <  1:3245002/150‑1 (MQ=255)

        gTGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCAAGGCGTCgta                                                                                                                                               <  1:7695694/150‑3 (MQ=255)

        gTGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCAAGGCGTCgta                                                                                                                                               <  1:50776/150‑3 (MQ=255)

         tGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCAAGGCGTCgtag                                                                                                                                              <  1:1095668/150‑4 (MQ=255)

              tGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAacac                                                                                                                                         >  2:6279839/1‑150 (MQ=255)

                   aCGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAg                                                                                                                                    >  1:7677144/1‑150 (MQ=255)

                     ggTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGt                                                                                                                                  >  2:8228508/1‑150 (MQ=255)

                     ggTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGt                                                                                                                                  >  1:5351247/1‑150 (MQ=255)

                        ggAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGt                                                                                                                               >  2:651769/1‑150 (MQ=255)

                         gAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTg                                                                                                                              <  1:6644277/150‑1 (MQ=255)

                         gAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTg                                                                                                                              >  1:1559410/1‑150 (MQ=255)

                              gCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGcc                                                                                                                         >  2:5222088/1‑150 (MQ=255)

                               cTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTGGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTCCGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCa                                                                                                                        <  2:5989412/150‑1 (MQ=255)

                                tggtggGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAg                                                                                                                       <  1:6241725/150‑1 (MQ=255)

                                tggtggGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAg                                                                                                                       <  2:1237487/150‑1 (MQ=255)

                                tggtggGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGGCGTGGCGCCAg                                                                                                                       >  2:5279424/1‑150 (MQ=255)

                                    gggtgTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCaa                                                                                                                   <  2:6876346/150‑1 (MQ=255)

                                    gggtgTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCaa                                                                                                                   >  2:3814353/1‑150 (MQ=255)

                                      gtgtTGACGGTGAGTCCAAGAACATCACCTTGCGACATGGGCTGGACTCGTGCGAGCCGCTGCTCAACCTTGACGACAACGGTGTCGAAATCCGCTAGTACTACGACGCCTTGCGTCGGGTATCAGGCGAGGTCGTGGCTCCAGTCaatc                                                                                                                 <  2:4695690/150‑3 (MQ=255)

                                       tgtTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGcc                                                                                                                >  2:1295116/1‑150 (MQ=255)

                                         ttGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCtt                                                                                                              <  2:7714883/150‑1 (MQ=255)

                                            aCGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCACGCCTTTCg                                                                                                           >  2:6454824/1‑150 (MQ=255)

                                            aCGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCg                                                                                                           >  2:761742/1‑150 (MQ=255)

                                              ggTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGaa                                                                                                         <  1:3481403/150‑1 (MQ=255)

                                                tGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGc                                                                                                       <  1:1831599/150‑1 (MQ=255)

                                                tGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGc                                                                                                       <  2:3294635/150‑1 (MQ=255)

                                                tGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGc                                                                                                       <  1:2031305/150‑1 (MQ=255)

                                                    tCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGaa                                                                                                   >  1:6918175/1‑150 (MQ=255)

                                                     ccAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAAc                                                                                                  >  1:3822859/1‑150 (MQ=255)

                                                     ccAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGACGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAAc                                                                                                  <  2:4681250/150‑1 (MQ=255)

                                                      cAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGATCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACg                                                                                                 <  2:3161304/150‑1 (MQ=255)

                                                      cAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACg                                                                                                 >  1:4921194/1‑150 (MQ=255)

                                                      cAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACg                                                                                                 >  2:6109126/1‑150 (MQ=255)

                                                       aaGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGc                                                                                                >  1:2482235/1‑150 (MQ=255)

                                                       aaGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGc                                                                                                >  2:120137/1‑150 (MQ=255)

                                                         gACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCaa                                                                                              >  1:6520892/1‑150 (MQ=255)

                                                         gACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCaa                                                                                              <  1:7907798/150‑1 (MQ=255)

                                                         gACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCaa                                                                                              <  1:5716410/150‑1 (MQ=255)

                                                            caccACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTa                                                                                           >  1:3976803/4‑150 (MQ=255)

                                                            cATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAAttc                                                                                           >  2:7103226/1‑149 (MQ=255)

                                                             aTCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTAc                                                                                          >  1:6375453/1‑150 (MQ=255)

                                                             aTCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTAc                                                                                          >  2:7506550/1‑150 (MQ=255)

                                                               cACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACaa                                                                                        <  2:4491320/150‑1 (MQ=255)

                                                               cACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACaa                                                                                        <  1:4751376/150‑1 (MQ=255)

                                                               cACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACaa                                                                                        <  2:3376515/150‑1 (MQ=255)

                                                                  cTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTa                                                                                     >  1:1008431/1‑150 (MQ=255)

                                                                  cTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTa                                                                                     >  2:2415318/1‑150 (MQ=255)

                                                                   ttGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCTCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTAc                                                                                    <  2:4894422/150‑1 (MQ=255)

                                                                   ttGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTAc                                                                                    >  2:6978675/1‑150 (MQ=255)

                                                                        aCATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCACTTACAAGTACTTCct                                                                               >  2:296413/1‑150 (MQ=255)

                                                                          aTGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCctct                                                                             >  2:7834799/1‑150 (MQ=255)

                                                                                tGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCAt                                                                       >  2:535946/1‑150 (MQ=255)

                                                                                 ggACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATa                                                                      <  2:2670911/150‑1 (MQ=255)

                                                                                    cacGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGa                                                                   <  1:5656233/150‑1 (MQ=255)

                                                                                    cacGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGa                                                                   <  2:7902755/150‑1 (MQ=255)

                                                                                       gggCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACaa                                                                >  1:8409351/1‑150 (MQ=255)

                                                                                        ggCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAAc                                                               <  2:140347/150‑1 (MQ=255)

                                                                                                 ctgctCAACCTCGACGACAACGGTGTCGAAAACCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAACTACTACCTCTGCGCATACGACAACGAGCAGGcc                                                      <  2:2238905/150‑1 (MQ=255)

                                                                                                        aCCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAAt                                               >  2:5637994/1‑150 (MQ=255)

                                                                                                         ccTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATg                                              >  1:7293807/1‑150 (MQ=255)

                                                                                                             gacgacAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCACGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGcc                                          >  2:8586041/1‑150 (MQ=255)

                                                                                                             gacgacAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACGTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGcc                                          <  2:7936606/150‑1 (MQ=255)

                                                                                                                gacAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCtgc                                       <  2:4869986/150‑3 (MQ=255)

                                                                                                                gacAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTAt                                       >  2:619708/1‑150 (MQ=255)

                                                                                                                gacAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTAt                                       >  2:8330607/1‑150 (MQ=255)

                                                                                                                   aaCGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGAc                                    <  2:345957/150‑1 (MQ=255)

                                                                                                                   aaCGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGAc                                    >  1:8556944/1‑150 (MQ=255)

                                                                                                                   aaCGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGAc                                    >  1:4427681/1‑150 (MQ=255)

                                                                                                                   aaCGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGAc                                    >  2:2890611/1‑150 (MQ=255)

                                                                                                                     cGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGt                                  >  2:4286869/1‑150 (MQ=255)

                                                                                                                      ggTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTg                                 >  1:3620321/1‑150 (MQ=255)

                                                                                                                      ggTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTg                                 >  1:7136537/1‑150 (MQ=255)

                                                                                                                       gtgtCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGa                                <  1:8794829/150‑1 (MQ=255)

                                                                                                                       gtgtCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGa                                <  1:6771380/150‑1 (MQ=255)

                                                                                                                        tgtCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGaa                               <  2:2054136/150‑1 (MQ=255)

                                                                                                                        tgtCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGaa                               <  1:5177883/150‑1 (MQ=255)

                                                                                                                        tgtCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGaa                               <  1:5941502/150‑1 (MQ=255)

                                                                                                                            gAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCaa                           >  2:1054302/1‑150 (MQ=255)

                                                                                                                            gAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCaa                           >  1:6309644/1‑150 (MQ=255)

                                                                                                                              aaTCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACGTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGt                         <  1:8179319/150‑1 (MQ=255)

                                                                                                                               aTCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGTGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTc                        <  2:549572/150‑1 (MQ=255)

                                                                                                                                tCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCg                       >  1:5495557/1‑150 (MQ=255)

                                                                                                                                 ccGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCga                      <  1:7496576/150‑1 (MQ=255)

                                                                                                                                 ccGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCga                      <  2:2338300/150‑1 (MQ=255)

                                                                                                                                     tactactacGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACg                  >  2:5878781/1‑150 (MQ=255)

                                                                                                                                           tacGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGcacaca            >  2:5747226/1‑150 (MQ=255)

                                                                                                                                             cgacgCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGCACACACt          >  1:8332313/1‑150 (MQ=255)

                                                                                                                                             cgacgCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGCACACACt          >  2:7276301/1‑150 (MQ=255)

                                                                                                                                             cgacgCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGCACACACt          >  1:4510041/1‑150 (MQ=255)

                                                                                                                                              gacgCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGCACACACTg         <  2:6024629/150‑1 (MQ=255)

                                                                                                                                                   cTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGCACACACTGCTCGa    >  2:1618804/1‑150 (MQ=255)

                                                                                                                                                    ttGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGCACACACTGCTCGAc   >  2:2824874/1‑150 (MQ=255)

                                                                                                                                                    ttGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGCACACACTGCTCGAc   <  1:3028416/150‑1 (MQ=255)

                                                                                                                                                     tGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACGACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGCACACACTGCTCGACg  <  1:2920922/150‑1 (MQ=255)

                                                                                                                                                     tGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGCACACACTGCTCGACg  <  1:3941315/150‑1 (MQ=255)

CGATGAGCGTGCACTGCAGACGGTGGAAAAGCTGGTGGGTGTTGACGGTGAGTCCAAGACCATCACCTTGCGACATGCCCTGGACACGGGCGAGCCGCTGCTCAACCTGGACGACAACGGTGTCGAAATCCGCTACTACTACGACGCCTTGCGTCGGGTAACACGCGAGGTCGTGGCGCCAGGCAAGCCTTTCGAAGCGGAACGCAATTACAAGTACTTCCTCTGCGCATACGACAACGAGCAGGCCCAGCAATGGGCCTATGACGTGAAGCAAGTCGAGACGCACACACTGCTCGACG  >  NC_002947/2131322‑2131620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`