BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,299,013	T→A	5.8%	L6M (TTG→ATG)	PP_2020 →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,299,013	0	T	A	5.8%	729.0 / 24.0	223	L6M (TTG→ATG)	PP_2020	lipoprotein
Reads supporting (aligned to +/- strand): ref base T (106/103); new base A (3/10); total (109/114)
Fisher's exact test for biased strand distribution p-value = 8.35e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

Only 100 of 228 total aligned reads displayed.

GGCCTGATGCTGACCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGCCGGCCTCACTGTCGAA  >  NC_002947/2298866‑2299162

ggCCTGATGCTGACCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTg                                                                                                                                                     >  1:1502254/1‑150 (MQ=255)

 gCCTGATGCTGACCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGc                                                                                                                                                    <  1:7118016/150‑1 (MQ=255)

    tGATGCTGACCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGc                                                                                                                                                 <  1:4645299/150‑1 (MQ=255)

    tGATGCTGACCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGc                                                                                                                                                 >  2:7968165/1‑150 (MQ=255)

      aTGCTGACCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGccc                                                                                                                                               <  1:7731804/150‑1 (MQ=255)

      aTGCTGACCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGccc                                                                                                                                               <  1:3257657/150‑1 (MQ=255)

        gCTGACCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCAATGCGGccccc                                                                                                                                             <  1:4842533/150‑1 (MQ=255)

         cTGACCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGcccccc                                                                                                                                            <  2:6408928/150‑1 (MQ=255)

          tGACCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCg                                                                                                                                           >  1:1390282/1‑150 (MQ=255)

          tGACCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCg                                                                                                                                           <  2:3134259/150‑1 (MQ=255)

          tGACCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCg                                                                                                                                           >  2:8367126/1‑150 (MQ=255)

          tGACCTTCATGCTGCTTGGGAACTTGTTTGGTGCGCTGGGGCTGCTACCGGCATTGGCGCGGTTCCTGATCACGCCTGAGAAGATGGTGGGCAAGGTGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCAGGGCGGCCCCCCg                                                                                                                                           <  1:5401055/150‑1 (MQ=255)

            aCCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGt                                                                                                                                         <  1:6647007/150‑1 (MQ=255)

              cTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTta                                                                                                                                       <  2:3472161/150‑1 (MQ=255)

              cTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTta                                                                                                                                       <  2:250022/150‑1 (MQ=255)

                tCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTtata                                                                                                                                     <  2:7673529/150‑1 (MQ=255)

                    gctgctTTGGAACATGTTTGGTGCGCTGTGGCTGCTACGGGCATTGGCGCGTTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGc                                                                                                                                 <  1:4110033/150‑1 (MQ=255)

                      tgctTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGTGGCCGCATTGCGGCACCCCGGTGATCCTGccc                                                                                                                               >  2:5825389/1‑149 (MQ=255)

                      tgctTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCa                                                                                                                               >  2:3069097/1‑150 (MQ=255)

                      tgctTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCa                                                                                                                               >  1:5843579/1‑150 (MQ=255)

                         tttGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCt                                                                                                                            >  1:8836/1‑150 (MQ=255)

                             gAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCAtt                                                                                                                        >  1:7589837/1‑150 (MQ=255)

                             gAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCAtt                                                                                                                        >  1:7503617/1‑150 (MQ=255)

                               aCATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTc                                                                                                                      <  2:418200/150‑1 (MQ=255)

                                    tttGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATg                                                                                                                 >  1:3981548/1‑150 (MQ=255)

                                    tttGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATg                                                                                                                 >  1:1991689/1‑150 (MQ=255)

                                     ttGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCATGGAGGGTGGGTCGATCTTCGCCCTTTGTTTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATgg                                                                                                                <  1:2416861/150‑1 (MQ=255)

                                         tGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTAc                                                                                                            >  2:4653579/1‑150 (MQ=255)

                                               gtgGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGTTGGTGGGCAAGGTGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCTCTTTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTAt                                                                                                      <  2:194245/150‑1 (MQ=255)

                                               gtgGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTAt                                                                                                      <  2:194670/150‑1 (MQ=255)

                                               gtgGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTAt                                                                                                      <  2:3744124/150‑1 (MQ=255)

                                                  gctgctACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTc                                                                                                   >  2:4461682/1‑150 (MQ=255)

                                                   ctgctACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTcc                                                                                                  <  2:3346055/150‑1 (MQ=255)

                                                    tgctACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTccc                                                                                                 >  1:2626192/1‑150 (MQ=255)

                                                    tgctACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCATATGTccc                                                                                                 >  2:4028913/1‑150 (MQ=255)

                                                       tACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTcccccc                                                                                              >  2:1760851/1‑150 (MQ=255)

                                                         ccGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTc                                                                                            <  1:7302539/150‑1 (MQ=255)

                                                          cGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCg                                                                                           >  2:5436246/1‑150 (MQ=255)

                                                           ggCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGc                                                                                          >  2:2125584/1‑150 (MQ=255)

                                                                  gcgcGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGcc                                                                                   <  1:3101150/150‑1 (MQ=255)

                                                                    gcgGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCt                                                                                 >  2:7730505/1‑150 (MQ=255)

                                                                    gcgGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCt                                                                                 <  2:5700179/150‑1 (MQ=255)

                                                                       gTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCa                                                                              >  1:2876650/1‑150 (MQ=255)

                                                                            tGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCt                                                                         >  2:4339775/1‑150 (MQ=255)

                                                                             gATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTg                                                                        >  1:6421395/1‑150 (MQ=255)

                                                                              aTCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCg                                                                                                                                                   <  2:4969230/74‑1 (MQ=255)

                                                                              aTCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCg                                                                                                                                                   >  1:4969230/1‑74 (MQ=255)

                                                                               tCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCg                                                                      >  1:5266634/1‑150 (MQ=255)

                                                                                cAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGa                                                                     <  1:2959216/150‑1 (MQ=255)

                                                                                cAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGa                                                                     <  1:6373510/150‑1 (MQ=255)

                                                                                 aaGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGat                                                                    <  2:6818191/150‑1 (MQ=255)

                                                                                   gCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGatat                                                                  >  1:6871148/1‑150 (MQ=255)

                                                                                   gCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGatat                                                                  <  2:8224189/150‑1 (MQ=255)

                                                                                    ccTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATg                                                                 >  1:1753847/1‑150 (MQ=255)

                                                                                      tGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCt                                                               >  1:1100368/1‑150 (MQ=255)

                                                                                      tGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCt                                                               >  1:6509087/1‑150 (MQ=255)

                                                                                       gagaAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCtt                                                              <  1:8389583/150‑1 (MQ=255)

                                                                                         gaagaTGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCGATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCtttt                                                            >  1:1705857/1‑150 (MQ=255)

                                                                                            gaTGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGAt                                                         <  2:4663042/150‑1 (MQ=255)

                                                                                            gaTGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGAt                                                         <  2:3580066/150‑1 (MQ=255)

                                                                                            gaTGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGAt                                                         <  2:5165185/150‑1 (MQ=255)

                                                                                            gaTGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGAt                                                         <  2:3983422/150‑1 (MQ=255)

                                                                                            gaTGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGAt                                                         <  2:3156695/150‑1 (MQ=255)

                                                                                                 tggGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACg                                                    <  2:690721/150‑1 (MQ=255)

                                                                                                 tggGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACg                                                    <  2:1037569/150‑1 (MQ=255)

                                                                                                  gggCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACAGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCAGCAGTCCTGCGATATGCGTTTGATCGACgg                                                   >  1:4389625/1‑150 (MQ=255)

                                                                                                   ggCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGc                                                  <  1:3425619/150‑1 (MQ=255)

                                                                                                      aaGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCGCCGGTTATAGTGCCAGCTGATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTg                                               >  2:1466745/1‑150 (MQ=255)

                                                                                                      aaGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTg                                               >  2:8460588/1‑150 (MQ=255)

                                                                                                      aaGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTg                                               >  1:1249232/1‑150 (MQ=255)

                                                                                                      aaGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTg                                               >  2:2661583/1‑150 (MQ=255)

                                                                                                      aaGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTg                                               >  1:4522311/1‑150 (MQ=255)

                                                                                                      aaGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTg                                               >  1:5330923/1‑150 (MQ=255)

                                                                                                        ggaggGTGGGTCGATCTTCGCGCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCa                                             >  2:6065766/1‑150 (MQ=255)

                                                                                                            ggtgggtCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGcc                                         >  2:288785/1‑150 (MQ=255)

                                                                                                                 gtCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGa                                    <  1:6672994/150‑1 (MQ=255)

                                                                                                                    gATCTTCGCCCATTGATTGGAGGGGCCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCCCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGAttt                                 <  2:740724/150‑1 (MQ=255)

                                                                                                                      tCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCa                               >  2:5368617/1‑150 (MQ=255)

                                                                                                                        ttCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCAcc                             >  2:6187296/1‑150 (MQ=255)

                                                                                                                         tCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCg                            >  1:8030303/1‑150 (MQ=255)

                                                                                                                           gCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGtt                          <  1:5713715/150‑1 (MQ=255)

                                                                                                                             ccATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTAACCGTtga                        >  2:5544062/1‑150 (MQ=255)

                                                                                                                             ccATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATAATGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCAACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGAATTCACCGTtga                        >  1:232387/1‑150 (MQ=255)

                                                                                                                              cATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTtgat                       <  1:4179542/150‑1 (MQ=255)

                                                                                                                                   attgGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGc                  >  1:8684618/1‑150 (MQ=255)

                                                                                                                                     accggggggCCGCAATGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGCCg                >  1:7844700/5‑150 (MQ=255)

                                                                                                                                     accggggggCCGCAATGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCAATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCGTCGATTTCACCGTTGATGAAGCCg                >  1:5230076/5‑150 (MQ=255)

                                                                                                                                      ggAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGCCgg               <  1:2561542/150‑1 (MQ=255)

                                                                                                                                       gAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGCCGGc              <  1:338516/150‑1 (MQ=255)

                                                                                                                                       gAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGCCGGc              <  2:6998283/150‑1 (MQ=255)

                                                                                                                                        aGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGCCGGcc             <  2:5777071/150‑1 (MQ=255)

                                                                                                                                        aGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGCCGGcc             <  2:2091087/150‑1 (MQ=255)

                                                                                                                                           gggCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGCCGGCCTCa          <  2:4858514/150‑1 (MQ=255)

                                                                                                                                              ccGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGCCGGCCTCACTg       >  2:7702/1‑150 (MQ=255)

                                                                                                                                              ccGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGCCGGCCTCACTg       >  1:1810956/1‑150 (MQ=255)

                                                                                                                                               cGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGCCGGCCTCACTGt      >  1:469752/1‑150 (MQ=255)

                                                                                                                                               cGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGCCGGCCTCACTGt      >  1:4764481/1‑150 (MQ=255)

                                                                                                                                               cGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGCCGGCCTCACTGt      >  2:3212660/1‑150 (MQ=255)

                                                                                                                                                gCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGCCGGCCTCACTGTc     <  2:3827152/150‑1 (MQ=255)

                                                                                                                                                   ttGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGCCGGCCTCACTGTCGaa  <  1:403191/150‑1 (MQ=255)

GGCCTGATGCTGACCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGCCGGCCTCACTGTCGAA  >  NC_002947/2298866‑2299162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`