Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 245,029 A→G 5.7% P235P (CCA→CCG algP → transcriptional regulator AlgP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947245,0290AG 5.7% 739.4 / 17.4 264P235P (CCA→CCGalgPtranscriptional regulator AlgP
Reads supporting (aligned to +/- strand):  ref base A (153/82);  new base G (2/13);  total (159/105)
Fisher's exact test for biased strand distribution p-value = 9.68e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

GCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCAAAACCAGCTGCCACCAAGGCACCAGCACGCAC  >  NC_002947/244880‑245178
                                                                                                                                                     |                                                                                                                                                     
        ccccgCGGCCAAACCGGCTGCCAATGCGACGGCTGCCGCCCATCGTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCaa                                                                                                                                               <  2:3070612/146‑1 (MQ=11)
          cTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAgg                                                                                                                                             >  2:5094873/1‑150 (MQ=34)
           tGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGc                                                                                                                                            >  1:6943647/1‑150 (MQ=34)
            gCGGCCAAACCGGCTGCCAAGGCGGCCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCg                                                                                                                                           >  1:2668814/1‑150 (MQ=21)
              ccccAAACCCGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGAGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAATCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGAc                                                                                                                                         <  2:7660912/148‑1 (MQ=255)
               gCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGAcc                                                                                                                                        >  2:8366146/1‑150 (MQ=34)
                ccAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAATCCTGCGGCCAAACCAGCTGCCAAGGCGAcgg                                                                                                                                       >  2:1388444/1‑148 (MQ=21)
                  aaaCCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCCGCTGCCAAGGCGACCGCt                                                                                                                                     >  2:6841690/1‑150 (MQ=17)
                     ccGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCCGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACAGCTGcc                                                                                                                                  >  1:121128/1‑150 (MQ=21)
                          tGCCAAGGCGACCGCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTaa                                                                                                                             >  2:7231934/1‑150 (MQ=17)
                              aaGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCt                                                                                                                         >  1:4657299/1‑150 (MQ=37)
                                 gCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCg                                                                                                                      >  2:2413861/1‑150 (MQ=37)
                                   gACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGc                                                                                                                    >  1:3084103/1‑150 (MQ=37)
                                             gccAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCt                                                                                                          >  1:821212/1‑150 (MQ=37)
                                               cAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGc                                                                                                        <  1:1699515/150‑1 (MQ=17)
                                                aaGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCAAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACAGCTGCAGCCAAGCCTGCGGCCAAACCAGCTGCCAAAGCGACAGCTGCCTCTAAGCCTGCGACAAAACCTGCAGcc                                                                                                       >  2:7279762/1‑150 (MQ=11)
                                                     tGCGGCCTAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAggc                                                                                                  <  1:7169513/150‑1 (MQ=37)
                                                     tGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAggc                                                                                                  <  1:5178203/150‑1 (MQ=37)
                                                     tGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAggc                                                                                                  >  2:6205889/1‑150 (MQ=37)
                                                         gCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCt                                                                                              >  1:7876287/1‑150 (MQ=39)
                                                          cccccccGGCCGCCCCGGCGCCCGCTCCCGCCACGCCCGCGGCCAACCCGGCTGCCAAGGCGCCCGCTGCCGCCAAGCCTGCGGCCACCCCCGCGGCCAAGGCGACCGCTGCCGCGAAGCCTGCGGCCAACCCCGCTGCCAAGGCGGCTg                                                                                             <  2:5628885/145‑1 (MQ=11)
                                                              aCCCGCTGCCAAGCCGACCCCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCCGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGc                                                                                         <  2:3457768/150‑1 (MQ=255)
                                                               ccGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCTGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCa                                                                                        <  1:3835291/150‑1 (MQ=255)
                                                               ccGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCa                                                                                        >  1:6434220/1‑150 (MQ=255)
                                                                cGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAg                                                                                       >  2:7365988/1‑150 (MQ=255)
                                                                 ggCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGc                                                                                      <  2:3527626/150‑1 (MQ=255)
                                                                    tGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGgccgccca                                                                                   >  2:609736/1‑148 (MQ=255)
                                                                      ccccggCGACCGCAGCCGCCAAGCCCGCGGCCAAACCGGCTGCCAAGCCGACCGCTGCCGCCAAGCCCGCGGCCAAACCAGCTGCCAAGGCGACCGCCGCCGCTACGCCTGCGGCCAAACCGGCCGCCAAGGCGGCTGCGGCAGCCaacc                                                                                 <  1:1036140/146‑3 (MQ=14)
                                                                       cAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCCGCCAAAcc                                                                                >  2:1446358/1‑150 (MQ=255)
                                                                       cAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAAcc                                                                                >  1:164099/1‑150 (MQ=255)
                                                                        aaGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCg                                                                                           <  2:461740/138‑1 (MQ=255)
                                                                         aGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTg                                                                              >  1:5570951/1‑150 (MQ=255)
                                                                          ggCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGc                                                                             >  1:2210872/1‑150 (MQ=255)
                                                                           gCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCg                                                                            <  1:3153501/150‑1 (MQ=255)
                                                                              aCCGCTGCTGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCt                                                                                                                                     <  2:5937472/90‑1 (MQ=18)
                                                                                cGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCaa                                                                       <  1:1471122/150‑1 (MQ=255)
                                                                                cGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCaa                                                                       >  1:6849691/1‑150 (MQ=255)
                                                                                 gCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAg                                                                      <  1:1146192/150‑1 (MQ=255)
                                                                                  cTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGc                                                                     <  1:5967008/150‑1 (MQ=255)
                                                                                  cTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGc                                                                     >  2:7616907/1‑150 (MQ=255)
                                                                                    gccgccAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCa                                                                   <  1:980483/150‑1 (MQ=255)
                                                                                     gcccacaGCCTTCGGCCACACGCCCCGCCTCGGCTACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAg                                                                  <  2:6712804/144‑1 (MQ=17)
                                                                                      cgccACTCCTGCGGCCATACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACTGCGGCAGCCAAACCTGCGGCCAAGCCAGc                                                                 <  1:2406573/150‑1 (MQ=255)
                                                                                       gccAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCt                                                                >  1:4518203/1‑150 (MQ=255)
                                                                                        ccAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTg                                                               >  2:333562/1‑150 (MQ=255)
                                                                                         cAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCAGCGGCCAAGCCAGCtcc                                                              >  2:1988025/1‑148 (MQ=255)
                                                                                          aaGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGcc                                                             >  1:3978232/1‑150 (MQ=255)
                                                                                             ccTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCaaa                                                          >  1:5016317/1‑150 (MQ=255)
                                                                                             ccTGCGCACAAACCGGCTGCCAAGGCTACCTCTGCAGCCTAGCCTGCGGCCAAAACAGCTGCCAAGGCGACCTCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCaaa                                                          <  2:3645780/150‑1 (MQ=17)
                                                                                              cTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAg                                                         >  1:3314262/1‑150 (MQ=255)
                                                                                              cTGCGGCCAAACCAGCTGCCATGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGACGACCACTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAg                                                         <  1:660324/150‑1 (MQ=11)
                                                                                               tGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGc                                                        >  2:2430887/1‑150 (MQ=255)
                                                                                                 cGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGc                                                                                                                    <  1:2170700/88‑1 (MQ=38)
                                                                                                     cAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCctgc                                                  <  2:5002554/150‑1 (MQ=255)
                                                                                                      aaaCCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAGGGCGGCTGCGGCAGCCAAACCTGCGGCCACGCCAGCTGCCAAAGCCCcggcg                                                 >  2:5311905/1‑149 (MQ=255)
                                                                                                      aaaCCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCctgct                                                 <  1:7818000/150‑1 (MQ=255)
                                                                                                       aaCCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCctgctg                                                <  1:5346716/150‑1 (MQ=255)
                                                                                                        aCCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCtgctgc                                               >  2:4856774/1‑150 (MQ=255)
                                                                                                        aCCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCtgctgc                                               >  1:6071331/1‑150 (MQ=255)
                                                                                                          cGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCa                                             >  2:3802407/1‑150 (MQ=255)
                                                                                                               gCCAGGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCAAAGCCTGCGGCCAAACCGGCTGCCAAggc                                                                                                  >  2:5986107/1‑92 (MQ=16)
                                                                                                               gCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAgccg                                        <  1:5752285/150‑1 (MQ=255)
                                                                                                                ccAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAgccgg                                       >  1:3941827/1‑150 (MQ=255)
                                                                                                                ccAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAgccgg                                       >  1:6170193/1‑150 (MQ=255)
                                                                                                                ccAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAgccgg                                       >  1:5945506/1‑150 (MQ=255)
                                                                                                                ccAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAgccgg                                       >  2:2300972/1‑150 (MQ=255)
                                                                                                                ccAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCAAAGCCTGCGGCCAAACCGGCTGCCAAGGCGCCAGCTGCAGCCAAACCTGCGGCCAATCCAGCTGCCAAAGCCCCTGCTGCCAAgccgc                                       >  1:2982843/1‑149 (MQ=17)
                                                                                                                 cAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAgccggc                                      <  1:7483554/150‑1 (MQ=255)
                                                                                                                  aaGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAgccggcc                                     >  2:1864399/1‑150 (MQ=255)
                                                                                                                   aGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCTAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGTCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAgccggccg                                    <  1:8618098/150‑1 (MQ=255)
                                                                                                                     gCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCg                                                                            <  2:1070942/108‑1 (MQ=255)
                                                                                                                     gCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCa                                  >  1:6307415/1‑150 (MQ=255)
                                                                                                                     gCGACCGCCGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCg                                                                            >  2:3865657/1‑108 (MQ=255)
                                                                                                                       gACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCaaa                                >  2:657779/1‑150 (MQ=255)
                                                                                                                        aCCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCaaaa                               >  1:4258479/1‑150 (MQ=255)
                                                                                                                        aCCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCaaaa                               >  1:2073416/1‑150 (MQ=255)
                                                                                                                        aCCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCaaaa                               >  2:2853036/1‑150 (MQ=255)
                                                                                                                           gCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCAAAACCa                            <  1:5156099/150‑1 (MQ=255)
                                                                                                                           gCTGCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGccc                                                      <  1:3505880/124‑1 (MQ=255)
                                                                                                                            cTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCAAAACCAg                           <  1:4473500/150‑1 (MQ=255)
                                                                                                                             tGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAACCCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCAAAACCAGc                          <  1:3609187/150‑1 (MQ=255)
                                                                                                                               ccgccAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCAAAACCAGCTg                        >  2:3953164/1‑150 (MQ=255)
                                                                                                                                cgccAAGCCTGCGGCCAAACCTGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCAAAACCAGCTGc                       <  1:1388444/150‑1 (MQ=255)
                                                                                                                                cgccAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCAAAACCAGCTGc                       <  1:4911058/150‑1 (MQ=255)
                                                                                                                                 gccAAGCCTGCTTCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCAAAACCAGCTGcc                      <  2:2694475/150‑1 (MQ=255)
                                                                                                                                   cAAGCCCGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCAAAACCAGCTGccac                    >  1:8141641/1‑150 (MQ=255)
                                                                                                                                      gCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCAAAACCAGCTGCCACCaa                 <  2:4727689/150‑1 (MQ=255)
                                                                                                                                       ccTGCGGCCAAACCCGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCAAAACCAGCTGCCACCAAg                <  1:2471844/150‑1 (MQ=255)
                                                                                                                                         tGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGc                                                                                                                           <  2:4099009/41‑1 (MQ=255)
                                                                                                                                         tGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCAAAACCAGCTGCCACCAAGGc              >  2:3290172/1‑150 (MQ=255)
                                                                                                                                             gCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCAAAACCAGCTGCCACCAAGGCACCa          >  2:606928/1‑150 (MQ=255)
                                                                                                                                              ccAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCAAAACCAGCTGCCACCAAGGCACCAg         >  1:127012/1‑150 (MQ=255)
                                                                                                                                               cAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCAAAACCAGCTGCCACCAAGGCACCAgc        >  1:8081908/1‑150 (MQ=255)
                                                                                                                                                aaaCCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCAAAACCAGCTGCCACCAAGGCACCAgca       >  1:7295367/1‑150 (MQ=255)
                                                                                                                                                aaaCCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCAAAACCAGCTGCCACCAAGGCACCAgca       >  2:1147760/1‑150 (MQ=255)
                                                                                                                                                 aaCCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCAAAACCAGCTGCCACCACGGCACCAgcac      >  2:163633/1‑150 (MQ=255)
                                                                                                                                                   ccAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCAAAACCAGCTGCCACCAAGGCACCAgcacgc    <  1:5637642/150‑1 (MQ=255)
                                                                                                                                                    cAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCAAAACCAGCTGCCACCAAGGCACCAgcacgca   >  1:3885762/1‑150 (MQ=255)
                                                                                                                                                    cAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCAAAACCAGCTGCCACCAAGGCACCAgcacgca   >  2:6366386/1‑150 (MQ=255)
                                                                                                                                                     aGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCAAAACCAGCTGCCACCAAGGCACCAgcacgcac  >  2:7932312/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCGCCAAACCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCGGCTGCCAAGGCGACCGCTGCCGCCAAGCCTGCGGCCAAACCAGCTGCCAAGGCGACCGCTGCCGCTAAGCCTGCGGCCAAACCGGCTGCCAAGGCGGCTGCGGCAGCCAAACCTGCGGCCAAGCCAGCTGCCAAAGCCCCTGCTGCCAAGCCGGCCGCAAAACCAGCTGCCACCAAGGCACCAGCACGCAC  >  NC_002947/244880‑245178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: