Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,549,4800CA44.0% 140.1 / inf 243intergenic (+48/+66)mdfA/ampGmultidrug MFS transporter/muropeptide permease AmpG
Reads supporting (aligned to +/- strand):  ref base C (40/96);  new base A (58/49);  total (98/145)
Fisher's exact test for biased strand distribution p-value = 1.25e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 257 total aligned reads displayed.

CTGCATTTTGCCCTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGA  >  NC_002947/1549331‑1549628
                                                                                                                                                     |                                                                                                                                                      
ctgcATTTTGCCCTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTacac                                                                                                                                                        >  2:7101680/1‑150 (MQ=255)
 tgcATTTTGCCCTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTAcaca                                                                                                                                                       >  2:3040111/1‑150 (MQ=255)
  gcttttgGCCCGGGGGGGGGGGGGGGCGGGGGCGTTGGCGCTGGGGCCGCTGTGGTTTGTGGGGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAg                                                                                                                                                      <  2:6093746/147‑1 (MQ=38)
  gcATTTTGCCGTGGCGGGGGGGGTGGCGGGGGCGTTGGCGGTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAg                                                                                                                                                      <  2:1635440/150‑1 (MQ=255)
    aTTTTGCCCGGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAggg                                                                                                                                                    <  2:488570/150‑1 (MQ=255)
        tGCCGTGGCGGGGGGGGGGGGGGGGGCGTTGGCGCTGGGGCCGCTGTGGTTTGGGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgccgc                                                                                                                                                <  1:2811647/150‑1 (MQ=39)
              ggcgggGGGGGGGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCggcgcggccct                                                                                                                                          <  1:6457914/150‑6 (MQ=25)
              ggcggcGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCggcgcggccct                                                                                                                                          <  2:5912577/150‑6 (MQ=255)
               gcggcgGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑tt                                                                                                                                        <  2:1475931/150‑1 (MQ=255)
                gggggggggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑ttt                                                                                                                                       <  1:5223560/146‑1 (MQ=255)
                cgggggGGGGGGGGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCggcgcggccctgt                                                                                                                                        <  1:810329/150‑8 (MQ=21)
                cgggggGGGGGGCGGGGGCGTGGGCGCTGTGGCGGCGGTGGTTTGGGGGGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑ttt                                                                                                                                       <  1:413115/150‑1 (MQ=39)
                cggcggGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑ttt                                                                                                                                       <  1:1713186/150‑1 (MQ=255)
                cggcggGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑ttt                                                                                                                                       <  2:2893867/150‑1 (MQ=255)
                cggcggGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGGGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑ttt                                                                                                                                       <  2:4672593/150‑1 (MQ=255)
                  gggggggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCC‑Tgtgt                                                                                                                                     <  2:4953838/148‑1 (MQ=37)
                  ctggggggggggggggCGTTGGCGCTGGGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGt                                                                                                                                     <  1:6586619/148‑1 (MQ=255)
                   gggggggggggggggggTTGGCGCTGTGGCCGCGGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGTa                                                                                                                                    <  1:6866479/149‑1 (MQ=255)
                   ggggggTGGGGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGTa                                                                                                                                    <  1:1020512/149‑1 (MQ=255)
                   ggggggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCC‑TGTGTa                                                                                                                                    <  1:7222041/149‑1 (MQ=37)
                   ggggggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCC‑TGTGTa                                                                                                                                    <  1:3915622/149‑1 (MQ=37)
                   cggGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGGGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGTa                                                                                                                                    <  1:5353260/150‑1 (MQ=255)
                   cggGGGGGGCGGGGGCGTGGGCGCTGGGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGTa                                                                                                                                    <  2:4135812/150‑1 (MQ=255)
                    ggggggggCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCC‑TGTGTAg                                                                                                                                   <  1:4789424/150‑1 (MQ=37)
                    gggggTGGGGGGGGGGTTGGCGCTGGGGCGGGTTTGGTTGGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTTGCGGGTAAACCCGCTCCTCCAAAGCGGCGCGGCCC‑TGTGTAg                                                                                                                                   <  1:1996480/150‑1 (MQ=21)
                    gggggTGGGGGGGGCGTTGGCGCTGTGGCCGCTGTTTTTTTTGTTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGTAg                                                                                                                                   <  1:461923/150‑1 (MQ=39)
                    gggggTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCC‑TGTGTAg                                                                                                                                   <  2:4306347/150‑1 (MQ=37)
                      ggggggCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGTAGGa                                                                                                                                 <  1:6669885/150‑1 (MQ=255)
                      ggggggCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGTAGGa                                                                                                                                 <  2:4244567/150‑1 (MQ=255)
                        ggggCGGGGGCGTGGGCGCGGTGGCCGCTGTGGTGTGGGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGc                                                                                                                               <  2:85871/148‑1 (MQ=25)
                        gTGGGGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGACGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGc                                                                                                                               <  1:4446975/150‑1 (MQ=25)
                        gTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGc                                                                                                                               <  2:6942399/150‑1 (MQ=37)
                          ggggggggCGTTGGCGCTGTGGCCGCGGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGC‑gg                                                                                                                            <  1:7761447/147‑1 (MQ=255)
                          ggggggggCGTGGGCGCGGTGGCCGCTGTGGTTTGTGGTGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGC‑gg                                                                                                                            <  1:3542212/147‑1 (MQ=255)
                          ggCGGGGGCGTTGGCGCTGTGGCCGCTGTGTTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑gg                                                                                                                            <  1:2025450/150‑1 (MQ=25)
                          ggCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGC‑gg                                                                                                                            <  1:5808165/150‑1 (MQ=255)
                          ggCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGC‑gg                                                                                                                            <  1:2833660/150‑1 (MQ=255)
                           ggggggggGTTGGCGCTGTGGCCGCTGTGTTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCAGCTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGC‑ggg                                                                                                                           <  1:7612474/148‑1 (MQ=255)
                           gCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGC‑ggg                                                                                                                           <  2:2347609/150‑1 (MQ=255)
                             gggggCTTTGGCGCTGGGGCCGCTGGGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGC‑GGGtt                                                                                                                         <  1:2348735/150‑1 (MQ=255)
                                   gTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCC‑TGTGTAg                                                                                                                                   >  2:4030164/1‑135 (MQ=25)
                                        cgcTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCACGTAGCGGGGAAACCCCCCCCAACACCCCGCCCCCCCCC‑CGTGTAGGGGCGGGGTTTACCcccgcg                                                                                                               >  1:3801801/1‑148 (MQ=255)
                                         gcggtgGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGATGGGGCTGTAGCTCGATAAGCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAaga                                                                                                             <  2:1761224/147‑1 (MQ=21)
                                          cTGTGGCCGCGGTGGTTGGGGGGGGGGGGGCGGCCGGGGGAAGGGGCTGTAGCTCGATAAGCGGTATTGCCGGGGCCGGCCTCTGCGGGGGAACACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGC‑GGGTTTACCCGCGAagag                                                                                                            <  2:5681895/150‑1 (MQ=37)
                                              ggcggcTGGGGTTTGTGGGGGGGGGGCGGCGGGGGGACGGGGCGGTAGCTCTATAAGGGGTTTGGCGGGGGCCGCCCTCTCCGGGGGTAACCCGGCTCCTACACTGGGCCGCGCGGA‑TTTTTAGGAGG‑GGTTTTACCCGCGAAGAGGCCa                                                                                                        <  2:3485739/150‑1 (MQ=255)
                                                    ggtggtgGGTGGGGGGGGGGGGGCCGGGGGACGGGGCGGTAGCGCGATAACCGTTATGGCGGGGGCCGGCCACTGCGGGGTAAAACCCGCTCCAACACCGGGCCGCGCCGC‑TTTGTAGGCGC‑GGGTTTACCCGCGAAGAGGCCAGCACAg                                                                                                  <  2:5472429/149‑1 (MQ=255)
                                                     gtgGTTGGTGGTGGGGGGGCGGCCGGGGGACGGGGCTGTAGCTCGAGAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTCCCCAGGGCCGCGCCGC‑TTTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAgg                                                                                                 <  2:7691520/150‑1 (MQ=37)
                                                        gTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCACTTAGCGGGTAAAACCCCCCCAAAACACGGCCCCCCCCC‑GTTTTAGGAGC‑GCGTTTAACCCCCAACAAGCCACCACAAGCaa                                                                                              >  2:6167880/1‑150 (MQ=255)
                                                              ggtgggtgGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTCCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACaaa                                                                                        <  2:3250596/150‑1 (MQ=255)
                                                              ggggggtgggCGGCCTGGGGACGGGGCTGTAGCTCGATACGCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACaaa                                                                                        <  2:7333862/147‑1 (MQ=255)
                                                                            cTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCACTTAGCGGGTAAAACCCCGCCATCACAGGGCCCCGCCCCGTTTGTGGGTCG‑GGTTTTACCCCCG‑AGAGGCCCGCACAGGCAAAAAAAAGCATAAGGGccccg                                                                          >  1:6479034/1‑147 (MQ=255)
                                                                              gggtttggggGTGTTGCTCGATACTGGGTTTGGCGGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCGGCGCCGT‑TTTGTAGGTGG‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCa                                                                        <  1:7071194/144‑1 (MQ=25)
                                                                                 gAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCACTTCGCCGGGAAAACCGCACCGAAACACGGCCCCCCCCC‑GTTGTAGGGGC‑CGGTTTTACCGCCAAGAGGCCACCCCCCGCAAAAAAAAACGTAAGGGCCCCGCAGcc                                                                     >  2:7257409/1‑150 (MQ=255)
                                                                                               tCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGt                                                                                                                                     >  2:1580573/1‑73 (MQ=255)
                                                                                               tCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGt                                                                                                                                     <  1:1580573/73‑1 (MQ=255)
                                                                                                      tCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCAACAAAGGGCCGCGCCGC‑GTTGTAGGCGC‑GGGGTTACCCCCCAAGAGGCCACCACACGCAAAACAAAGCGTCAGGGCCGCTCAGCCACCCAGTGGTCGTCCGCCAcc                                                >  2:5088382/1‑150 (MQ=25)
                                                                                                      tCGGTATTGCCGGGGCCGGCCTCTTCGCGGGGAAAACCCCGACCACACCGGGGCCCGCCGC‑GGTGTGGGGGC‑GGGGTTAACCCCGCAGAGGCCCGCCCCGGCCAAACAAAGCATCAGGGCCCCGCACCCCCCCAGGGGTCGTCCGCcccc                                                >  2:651302/1‑150 (MQ=255)
                                                                                                         gTATTGCGGGGGCCGGCCTCTTCGGGGGTAAACCCGCTCCTACACCGGGCCGCGCGGC‑TTTGTAGGCGC‑GGTTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGc                                             <  2:2813111/150‑1 (MQ=38)
                                                                                                                    gccggccTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCcccgccc                                  <  2:3727872/150‑1 (MQ=255)
                                                                                                                      cggccTCTTCGCGGGTAAACCCGCTCCAACACAGGGCCGCGCCCC‑TTTGTTGGAGC‑GGGTTTACCCCCGAAGAGGCCAGCACAGGCAATACAAAACATCAGGGCCGCGCAGCCACCGAGTTGTCGTCCGCCACCGGCGTCcccccccccg                                >  1:1217664/1‑148 (MQ=25)
                                                                                                                          ctctTGGGGGTTAAACCCGCTCCTACACCGGGCCGCGCCGC‑TTTGTTGGCGG‑GGTTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTc                            <  1:4964449/150‑1 (MQ=255)
                                                                                                                            ctTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCt                          <  2:7377771/150‑1 (MQ=255)
                                                                                                                             ttCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTg                         >  2:1925507/1‑150 (MQ=25)
                                                                                                                               cgcgGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGAt                       <  2:3822800/150‑1 (MQ=255)
                                                                                                                                 cgGGTAAACCCGCTCCTACACCGGGCCGCGCCGC‑TTGGTAGGAGC‑GGTTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGc                     <  2:1999823/150‑1 (MQ=255)
                                                                                                                                 cgGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGc                     <  2:1768839/150‑1 (MQ=255)
                                                                                                                                   ggTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCa                   >  2:2405017/1‑150 (MQ=255)
                                                                                                                                         cccGCTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAg             >  2:7487996/1‑150 (MQ=255)
                                                                                                                                         cccGCTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAg             >  1:4254309/1‑150 (MQ=37)
                                                                                                                                          ccGCTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg            >  1:624772/1‑150 (MQ=255)
                                                                                                                                          ccGCTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg            >  2:3368473/1‑150 (MQ=255)
                                                                                                                                          ccGCTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGAt                       <  1:5471665/139‑1 (MQ=25)
                                                                                                                                          ccGCTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGAt                       >  2:5471665/1‑139 (MQ=25)
                                                                                                                                          ccGCTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg            >  2:584733/1‑150 (MQ=37)
                                                                                                                                          ccGCTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg            >  1:2970878/1‑150 (MQ=37)
                                                                                                                                          ccGCTCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTATACCCGCGAAGAGGCCGGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg            >  2:3621615/1‑150 (MQ=25)
                                                                                                                                              tCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCt                                                                          <  2:902632/84‑1 (MQ=14)
                                                                                                                                              tCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCt                                                                          >  1:902632/1‑84 (MQ=14)
                                                                                                                                              tCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTcc                           >  2:152659/1‑131 (MQ=25)
                                                                                                                                              tCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAggg           <  2:557868/147‑1 (MQ=37)
                                                                                                                                              tCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAggg           >  1:557868/1‑147 (MQ=37)
                                                                                                                                              tCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCga        >  2:2484094/1‑150 (MQ=37)
                                                                                                                                              tCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCga        >  2:7764556/1‑150 (MQ=37)
                                                                                                                                              tCCTACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCACTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCga        >  1:3335123/1‑150 (MQ=37)
                                                                                                                                                cTACACAGGGCCGCGCCGC‑TTTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatc      >  1:7551440/1‑150 (MQ=255)
                                                                                                                                                cTACACAGGGCCGCGCCGC‑TTTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatc      >  2:139907/1‑150 (MQ=255)
                                                                                                                                                cTACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatc      >  2:7054182/1‑150 (MQ=37)
                                                                                                                                                cTACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatc      >  2:812013/1‑150 (MQ=37)
                                                                                                                                                cTACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatc      >  2:3177290/1‑150 (MQ=37)
                                                                                                                                                 tACACAGGGCCGCGCCGC‑TTTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGT‑CCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatcag    >  2:445767/1‑150 (MQ=255)
                                                                                                                                                 tACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCGGCCCCGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca     >  1:3727008/1‑150 (MQ=25)
                                                                                                                                                 tACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca     >  2:1194731/1‑150 (MQ=37)
                                                                                                                                                 tACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca     >  2:1412777/1‑150 (MQ=37)
                                                                                                                                                 tACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca     >  2:1889200/1‑150 (MQ=37)
                                                                                                                                                 tACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca     >  2:7506528/1‑150 (MQ=37)
                                                                                                                                                 tACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca     >  2:5740756/1‑150 (MQ=37)
                                                                                                                                                 tACAAAGCGGCGCGGCCC‑TGTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca     >  1:2232834/1‑150 (MQ=37)
                                                                                                                                                  acacAGGGCCGCGCCGC‑TTTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCAcc                                                >  2:6194095/1‑106 (MQ=255)
                                                                                                                                                  acacAGGGCCGCGCCGC‑TTTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCAcc                                                <  1:6194095/106‑1 (MQ=255)
                                                                                                                                                    acaGGGCCGCGCCGC‑TTTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatcagga  >  2:2299083/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CTGCATTTTGCCCTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGC‑TTTGTAGGAGC‑GGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGA  >  NC_002947/1549331‑1549628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑