| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,167,910:1 | +A | 100% | coding (723/1344 nt) | oprB‑I → | carbohydrate‑selective porin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,167,910 | 1 | . | A | 91.3% | 62.3 / 0.8 | 23 | coding (723/1344 nt) | oprB‑I | carbohydrate‑selective porin |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base A (8/13); total (8/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGGTCAGCCAGTGGGCGCTGCGGGTGAAGTACAACATCACGCCGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACC‑A‑AGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCGGGCGAGTACCGCCTGGGTTACTACTACAGCACGGCCAAGGCTGAC > NC_002947/1167780‑1168012 | cGGTCAGCCAGTGGGCGCTGCGGGTGAAGTACAACATCACGCCGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACC‑A‑Aagg < 2:453136/136‑1 (MQ=255) gcTGCGGGTGAAGTACAACATCACGCCGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAA‑AGGGGGCGATCTTGCCGGt < 1:237963/136‑1 (MQ=255) aGTACAACATCACGCCGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAA‑AGGGGGCGATCTTGCCGGTGGAAGCGgtgt < 1:175236/136‑1 (MQ=255) aacaTCACGCCGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAA‑AGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCg < 2:275676/136‑1 (MQ=255) acaTCACGCCGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAA‑AGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGc < 2:497012/136‑1 (MQ=255) acaTCACGCCGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAA‑AGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGc < 2:416750/136‑1 (MQ=255) cGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAA‑AGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCa > 2:457040/1‑136 (MQ=255) gAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACGCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAA‑AGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAAt < 2:109326/136‑1 (MQ=255) gTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAA‑AGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCg > 1:310035/1‑136 (MQ=255) tACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAA‑AGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCgg < 2:310035/136‑1 (MQ=255) aCAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAA‑AGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCggg > 2:468410/1‑136 (MQ=255) aGGTCGGGGCCTTCGAGCAGAACCCTTGCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAA‑AGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCGGGCg < 2:249067/136‑1 (MQ=255) ggggCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAA‑AGGGGGCGATCTTGCCGGTGGAAGCGgtgt < 2:89662/101‑1 (MQ=255) ggggCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAA‑AGGGGGCGATCTTGCCGGTGGAAGCGgtgt > 1:89662/1‑101 (MQ=255) ggggCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAA‑AGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCGGGCGAGTAc > 2:460139/1‑136 (MQ=255) ccTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAA‑AGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCACCCAAGGTCAATGGCCTGCCGGGCGAGTACCGcc < 2:323273/136‑1 (MQ=255) gAGTAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAA‑AGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCGGGCGAGTACCGCCTGGGt < 1:276536/136‑1 (MQ=255) aGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAA‑AGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCGGGCGAGTACCGCCTGGGtt > 1:374869/1‑136 (MQ=255) cAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAA‑AGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCGGGCGAGTACCGCCTGGGTTACTACTACAGCAc > 1:272652/1‑136 (MQ=255) tGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAA‑AGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCGGGCGAGTACCGCCTGGGTTACTACTACAGCACGGCCa > 1:360900/1‑136 (MQ=255) aaaCCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAA‑AGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCGGGCGAGTACCGCCTGGGTTACTACTACAGCACGTCCAAgg < 1:468410/136‑1 (MQ=255) aaaCCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACCAA‑AGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCGGGCGAGTACCGCCTGGGTTACTACTACAGCACGGCCAAgg < 2:374869/136‑1 (MQ=255) ggCAACGGCTTCAAGCTCAGCGGCAGTGGTACG‑ATAGGGGGCGATCTTGCGGGTGGATGCGGTGTGGTCGCCCAAGGTCATTGGCCTGCCTGGCGAGTACCGCGTGGGTTACTACTACAGCACGGCCAATGCTTAc < 2:421325/136‑1 (MQ=255) | CGGTCAGCCAGTGGGCGCTGCGGGTGAAGTACAACATCACGCCGGAGTTCTTCGTACAGGTCGGGGCCTTCGAGCAGAACCCTTCCAACCTGGAAACCGGCAACGGCTTCAAGCTCAGCGGCAGTGGTACC‑A‑AGGGGGCGATCTTGCCGGTGGAAGCGGTGTGGTCGCCCAAGGTCAATGGCCTGCCGGGCGAGTACCGCCTGGGTTACTACTACAGCACGGCCAAGGCTGAC > NC_002947/1167780‑1168012 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |