Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,153 G→T 15.6% R113S (CGT→AGT)  rsmG ← 16S RNA methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,1530GT15.6% 95.2 / 3.2 31R113S (CGT→AGT) rsmG16S RNA methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (18/8);  new base T (2/3);  total (20/11)
Fisher's exact test for biased strand distribution p-value = 3.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTTCTCCATGCTGCTGAAGGCGCGGGAGATGATTCCGTCGAACGGTTGTGCCGGCTGGAAGGCCTCGACCCGGCTGTGGATAACCGTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGTCTTCTTGCCATTGGCGTCCAGCACGGTGACGCGCTTGTGTGGATGCAGGATAGCCAGCGGGATGCCGGGCATGCCACCGCCGCTGCCAACATCCAGCCAGTTATCACGATCGTTGTGGATAAAAGA  >  NC_002947/2021‑2282
                                                                                                                                    |                                                                                                                                 
aGTTCTCCATGCTGCTGAAGGCGCGGGAGATGATTCCGTCGAACGGTTGTGCCGGCTGGAAGGCCTCGACCCGGCTGTGGATAACCGTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACgggg                                                                                                                                <  2:357210/136‑2 (MQ=255)
  ttCTCCATGCTGCTGAAGGCGCGGGAGATGATTCCGTCGAACGGTTGTGCCGGCTGGAAGGCCTCGACCCGGCTGTGGATAACCGTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGtct                                                                                                                              <  2:468081/136‑1 (MQ=255)
          gctgctGAAGGCGCGGGAGATGATTCCGTCGAACGGTTGTGCCGGCTGGAAGGCCTCGACCCGGCTGTGGATAACCGTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGTCTTCTTGCCa                                                                                                                      >  2:384153/1‑136 (MQ=255)
                 aaGGCGCGGGAGATGATTCCGTCGAACGGTTGTGCCGGCTGGAAGGCCTCGACCTGGCTGTGGATAACCGTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGTCTTCTTGCCATTGGCGt                                                                                                               >  1:419110/1‑136 (MQ=255)
                 aaGGCGCGGGAGATGATTCCGTCGAACGGTTGTGCCGGCTGGAAGGCCTCGACCCGGCTGTGGATAACCGTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGTCTTCTTGCCATTGGCGt                                                                                                               >  1:129667/1‑136 (MQ=255)
                           agaTGATTCCGTCGAACGGTTGTGCCGGCTGGAAGGCCTCGACCCTGCTGTGGATAACCGTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACTGGTCTTCTTGCCATTGGCGTCCAGCACGGt                                                                                                     >  1:122131/1‑136 (MQ=255)
                           agaTGATTCCGTCGAACGGTTGTGCCGGCTGGAAGGCCTCGACCCTGCTGTGGATAACCGTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACTGGTCTTCTTGCCATTGGCGTCCAGCACGGt                                                                                                     >  1:455372/1‑136 (MQ=255)
                           agaTGATTCCGTCGAACGGTTGTGCCGGCTGGAAGGCCTCGACCCGGCTGTGGATAACCGTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGTCTTCTTGCCATTGGCGTCCAGCACGGt                                                                                                     >  2:464238/1‑136 (MQ=255)
                           agaTGATTCCGTCGAACGGTTGTGCCGGCTGGAAGGCCTCGACCCGGCTGTGGATAACCGTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGTCTTCTTGCCATTGGCGTCCAGCACGGt                                                                                                     >  2:461907/1‑136 (MQ=255)
                                     gTCGAACGGTTGTGCCGGCTGGAAGGCCTCGACCCGGCTGTGGATAACCGTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGt                                                                                                                                <  2:334550/99‑1 (MQ=255)
                                     gTCGAACGGTTGTGCCGGCTGGAAGGCCTCGACCCGGCTGTGGATAACCGTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGt                                                                                                                                >  1:334550/1‑99 (MQ=255)
                                       cGAACGGTTGTGCCGGCTGGAAGGCCTCGACCCGGCTGTGGATAACCGTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGTCTTCTTGCCATTGGCGTCCAGCACGGTGACGCGCTtgtg                                                                                         >  1:126799/1‑136 (MQ=255)
                                         aaCGGTTGTGCCGGCTGGAAGGCCTCGACCCGGCTGTGGATAACCGTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGTCTTCTTGCCATTGGCGTCCa                                                                                                            <  2:289739/115‑1 (MQ=255)
                                         aaCGGTTGTGCCGGCTGGAAGGCCTCGACCCGGCTGTGGATAACCGTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGTCTTCTTGCCATTGGCGTCCa                                                                                                            >  1:289739/1‑115 (MQ=255)
                                         aaCGGTTGTGCCGGCTGGAAGGCCTCGACCCGGCTGTGGATAACCGTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGTCTTCTTGCCATTGGCGTCCAGCACGGTGACGCGCTtgtgtg                                                                                       <  1:137381/136‑1 (MQ=255)
                                              gtgtgCCGGCTGGAAGGCCTCGACCCGGCTGTGGATAACCTTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACTGGTCTTCTTGACATTGGCGTCCAGCACGGTGACGCGCTTGTGTGGATGc                                                                                  <  1:63293/135‑1 (MQ=255)
                                                   ccGGCTGGAAGGCCTCGACCCGGCTGTGGATAACCGTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGTCTTCTTGCCATTGGCGTCCAGCACGGTGACGCGCTTGTGTGGATGCAGGAt                                                                             >  1:428214/1‑136 (MQ=255)
                                                           aaGGCCTCGACCCGGCTGTGGATAACCGTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGTCTTCTTGCCATTGGCGTCCAGCACGGTGACGCGCTTGTGTGGATGCAGGATAGCCAGCg                                                                     >  1:237892/1‑136 (MQ=255)
                                                                   gACCCGGCTGTGGATAACCGTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGTCTTCTTGCCATTGGCGTCCAGCACGGTGACGCGCTTGTGTGGATGCAGGATAGCCAGCGGGATGCCg                                                             >  2:462034/1‑136 (MQ=255)
                                                                            gtgGATAACCGTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGTCTTCTTGCCATTGGCGTCCAGCACGGTGACGCGCTTGTGTGGATGCAGGATAGCCAGCGGGATGCCGGGCATGCCa                                                    >  1:457030/1‑136 (MQ=255)
                                                                               gATAACCGTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGTCTTCTTGCCATTGGCGTCCAGCACGGTGACGCGCTTGTGTGGATGCAGGATAGCCAGCGGGATGCCGGGCATGCCAccg                                                 <  2:319564/136‑1 (MQ=255)
                                                                                  aaCCGTGAGGTTGTCCAGCTTGAGTTCCATTTTCACATGAGTCAGGAAACGGGTCTTCTTGACATTGGCGTCCAGCACGGTGACGCGCTTGGGTGGATGCAGGATAGCCAGCTGGATGCCGGGCATGCCAccgccc                                              >  1:310586/1‑135 (MQ=255)
                                                                                      gTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGTCTTCTTGCCATTGGCGTCCAGCACGGTGACGCGCTTGTGTGGATGCAGGATAGCCAGCGGGATGCCGGGCATGCCACCGCCGCTGc                                          >  2:443471/1‑136 (MQ=255)
                                                                                          ggTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGTCTTCTTGCCATTGGCGTCCAGCACGGTGACGCGCTTGTGTGGATGCAGGATAGCCAGCGGGATGCCGGGCATGCCACCGCCGCTGCCAAc                                      >  1:379626/1‑136 (MQ=255)
                                                                                          ggTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGTCTTCTTGCCATTGGCGTCCAGCACGGTGACGCGCTTGTGTGGATGCAGGATAGCCAGCGGGATGCCGGGCATGCCACCGCCGCTGCCAAc                                      >  1:205058/1‑136 (MQ=255)
                                                                                          ggTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGTCTTCTTGCCATTGGCGTCCAGCACGGTGACGCGCTTGTGTGGATGCAGGATAGCCAGCGGGATGCCGGGCATGCCACCGCCGCTGCCAAc                                      >  2:400252/1‑136 (MQ=255)
                                                                                                 cAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGTCTTCTTGCCATTGGCGTCCAGCACGGTGACGCGCTTGTGTGGATGCAGGATAGCCAGCGGGATGCCGGGCATGCCACCGCCGCTGCCAACATccagc                               >  1:134385/1‑136 (MQ=255)
                                                                                                                      cTGAGTCAGGAAACGGGTCTTCTTGCCATTGGCGTCCAGCACGGTGACGCGCTTGTGTGGATGCAGGATAGCCAGCGGGATGCCGGGCATGCCACCGCCGCTGCCAACATCCAGCCAGTTATCACGATCGTTGTgg          <  2:457030/136‑1 (MQ=255)
                                                                                                                             aGGAAACTGGTCTTCTTGCCATTGGCGTCCAGCACGGTGACGCGCTTGTGTGGATGCAGGATAGCCAGCGGGATGCCGGGCATGCCACCGCCGCTGCCAACATCCAGCCAGTTATCACGATCGTTGTGGATAAAAg   <  2:455372/136‑1 (MQ=255)
                                                                                                                             aGGAAACTGGTCTTCTTGCCATTGGCGTCCAGCACGGTGACGCGCTTGTGTGGATGCAGGATAGCCAGCGGGATGCCGGGCATGCCACCGCCGCTGCCAACATCCAGCCAGTTATCACGATCGTTGTGGATAAAAg   <  2:122131/136‑1 (MQ=255)
                                                                                                                              ggAAACGGGTCTTCTTGCCATTGGCGTCCAGCACGGTGACGCGCTTGTGTGGATGCAGGATAGCCAGCGGGATGCCGGGCATGCCACCGCCGCTGCCAACATCCAGCCAGTTATCACGATCGTTGTGGATAAAAGa  <  1:464238/136‑1 (MQ=255)
                                                                                                                              ggAAACGGGTCTTCTTGCCATTGGCGTCCAGCACGGTGACGCGCTTGTGTGGATGCAGGATAGCCAGCGGGATGCCGGGCATGCCACCGCCGCTGCCAACATCCAGCCAGTTATCACGATCGTTGTGGATAAAAGa  <  1:461907/136‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                 
AGTTCTCCATGCTGCTGAAGGCGCGGGAGATGATTCCGTCGAACGGTTGTGCCGGCTGGAAGGCCTCGACCCGGCTGTGGATAACCGTGAGGTTGTCCAGCTTGAGTTCCATTTTCACCTGAGTCAGGAAACGGGTCTTCTTGCCATTGGCGTCCAGCACGGTGACGCGCTTGTGTGGATGCAGGATAGCCAGCGGGATGCCGGGCATGCCACCGCCGCTGCCAACATCCAGCCAGTTATCACGATCGTTGTGGATAAAAGA  >  NC_002947/2021‑2282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑