| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,751,312 | C→A | 21.0% | D232Y (GAC→TAC) | etfA ← | electron transfer flavoprotein subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,751,312 | 0 | C | A | 21.0% | 46.6 / 3.3 | 19 | D232Y (GAC→TAC) | etfA | electron transfer flavoprotein subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (4/11); new base A (2/2); total (6/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01 | |||||||||||
CTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTACAGGTGCTTGAAGTTGTCACCGTTACCCATGCCACGGCCGCCGGAAACGACGATTTTGGCAGCGGTCAGCTC > NC_002947/4751195‑4751438 | cTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGc < 2:96792/136‑1 (MQ=255) tCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTAGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTc > 1:170148/1‑136 (MQ=255) tCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTAGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTc > 1:172428/1‑136 (MQ=255) gcgcCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCgg < 1:259015/136‑1 (MQ=255) gcgcCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCgg < 1:162801/136‑1 (MQ=255) gcgcCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCgg < 2:484943/136‑1 (MQ=255) gcgcCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCgg < 2:167880/136‑1 (MQ=255) gcgcCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCgg < 1:389663/136‑1 (MQ=255) gcCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTAGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGcc < 2:170148/136‑1 (MQ=255) gcCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTAGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGcc < 2:172428/136‑1 (MQ=255) cGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAg > 2:360997/1‑136 (MQ=255) aTACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGt > 2:23868/1‑136 (MQ=255) aTACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGt > 2:426581/1‑136 (MQ=255) tACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTa < 2:312476/136‑1 (MQ=255) tACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTACAGGTGCTTGAAGt > 2:30395/1‑136 (MQ=255) tACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTACAGGTGCTTGAAGt < 1:30395/136‑1 (MQ=255) gTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTACAGGTGCTTGAAGTTGTCACCGTTACCCATGCCACGGCCGCCGGAAACGACGATTTTg < 2:266338/136‑1 (MQ=255) gCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTACAGGTGCTTGAAGTTGTCACCGTTACCCATGCCACGGCCGCCGGAAACGACGATTTTGGCAGCGGt < 1:360997/136‑1 (MQ=255) aGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTAGAGGTGCTTGAAGTTGTCACCGTTACCCATGCCACGGCCGCCGGAAACGACGATTTTGGCAGCGGTCAGctc < 2:302545/136‑1 (MQ=255) | CTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCCGGTCTGGCCGACCTGCATGTCGTTCGGCACGAAGCCTGCGTCGACTGCGGCGCGCGAAGCACCAACCGCAGCGCCGAGCTTGTCGGCCAGGCTGTACAGGTGCTTGAAGTTGTCACCGTTACCCATGCCACGGCCGCCGGAAACGACGATTTTGGCAGCGGTCAGCTC > NC_002947/4751195‑4751438 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |