| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,104,416 | G→T | 30.8% | intergenic (‑28/‑241) | acsA‑I ← / → PP_4488 | acetyl‑CoA synthetase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,104,416 | 0 | G | T | 30.8% | 16.8 / 4.1 | 13 | intergenic (‑28/‑241) | acsA‑I/PP_4488 | acetyl‑CoA synthetase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (2/7); new base T (2/2); total (4/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01 | |||||||||||
GTTGTTTTTGTATGGAACCGTTTGTAACTGTACCAGAGCGACAAAAGCCATTCGACGTTGGTAGTAATTTGGTACACCCGTTCCGGCAAAACGCTCTGGCGCGGGCATTACAGCCTTGCCGACGAAAAAACGAGGGGTTTGCCACAGGATTGTTACAGATTCTGTCAAAACACTTTATCAAAACACCCACTGTTTCGGCCTTGGCCAGCGCCATAAAATTCACCTCGCCAGCAACGGCAAGGCGATTAACAACTGGTAACAGCCCCC > NC_002947/5104281‑5104547 | gttgttTTTGTATGGAACCGTTTGTAACTGTACCAGAGCGACAAAAGCCATTCGACGTTGGTAGTAATTTGGTACACCCGTTCCGGCAAAACGCTCTGGCGCGGGCATTACAGCCTTGCCGACGAAAAAACGAggg < 1:181767/136‑1 (MQ=255) tttGTATGGAACCGTTTGTAACTGTACCAGAGCGACAAAAGCCATTCGACGTTGGTAGTAATTTGGTACACCCGTTCCGGCAAAACGCTCTGGCGCGGGCATTACAGCCTTGCCGACGAAAAAACGAGGTGTTTGc > 1:335941/1‑136 (MQ=255) tttGTATGGAACCGTTTGTAACTGTACCAGAGCGACAAAAGCCATTCGACGTTGGTAGTAATTTGGTACACCCGTTCCGGCAAAACGCTCTGGCGCGGGCATTACAGCCTTGCCGACGAAAAAACGAGGGGTTTGc > 1:113791/1‑136 (MQ=255) tATGGAACCGTTTGTAACTGTACCAGAGCGACAAAAGCCATTCGACGTTGGTAGTAATTTGGTACACCCGTTCCGGCAAAACGCTCTGGCGCGGGCATTACAGCCTTGCCGACGAAAAAACGAGGGGTTTGCcaca < 1:313238/136‑1 (MQ=255) gTAACTGTACCAGAGCGTCAAAATCCATTCGACGTTGTTAGTAATTTGGTACACCCGTTCCGGCAAAACACTCTGGCGCGGGCATTACAGCCTTGCCGACGAAAAAACGAGGGGTTTGCCACAGGATTGTTACAGa < 2:496709/136‑1 (MQ=255) cGACAAAAGCCATTCGACGTTGGTAGTAATTTGGTACACCCGTTCCTGCAAAACGCTCTGGCGCGGGCATTACAGCCTTGCCGACGAAAAAACGAGGGGTTTGCCACAGGATTGTTACAGATTCTGTCAAAACACt < 1:51395/136‑1 (MQ=255) aaaGCCATTCGACGTTGGTAGTAATTTGGTACACCCGTTCCGGCAAAACGCTCTGGCGCGGGCATTACAGCCTTGCCGACGAAAAAACGAGGTGTTTGCCACAGGATTGTTACAGATTCTGTCAAAACACTTTATc > 2:436624/1‑136 (MQ=255) aaaGCCATTCGACGTTGGTAGTAATTTGGTACACCCGTTCCGGCAAAACGCTCTGGCGCGGGCATTACAGCCTTGCCGACGAAAAAACGAGGGGTTTGCCACAGGATTGTTACAGATTCTGTCAAAACACTTTATc < 2:430355/136‑1 (MQ=255) tGGTACACCCGTTCCGGCAAAACGCTCTGGCGCGGGCATTACAGCCTTGCCGACGAAAAAACGAGGTGTTTGCCACAGGATTGTTACAGATTCTGTCAAAACACTTTATCAAAACACCCACTGTTTCGGCCTTGGc < 2:335941/136‑1 (MQ=255) tGGTACACCCGTTCCGGCAAAACGCTCTGGCGCGGGCATTACAGCCTTGCCGACGAAAAAACGAGGGGTTTGCCACAGGATTGTTACAGATTCTGTCAAAACACTTTATCAAAACACCCACTGTTTCGGCCTTGGc < 2:113791/136‑1 (MQ=255) cgacgaAAAAACGAGGGGTTTGCCACAGGATTGTTACAGATTCTGTCAAAACACTTTATCAAAACACCCACTGTTTCGGCCTTGGCCAGCGCCATAAAATTCACCTCGCCAGCAACGGCAAGGCGATTAACAACTg > 1:169728/1‑136 (MQ=255) acgaAAAAACGAGGGGTTTGCCACAGGATTGTTACAGATTCTGTCAAAACACTTTATCAAAACACCCACTGTTTCGGCCTTGGCCAGCGCCATAAAATTCACCTCGCCAGCAACGGAAAGGCTATTAACAACTGGt < 2:4382/136‑1 (MQ=255) aaaaaCGAGGGGTTTGCCACAGGATTGTTACAGATTCTGTCAAAACACTTTATCAAAACACCCACTGTTTCGGCCTTGGCCAGCGCCATAAAATTCACCTCGCCAGCAACGGCAAGGCGATTAACAACTGGTAACa < 1:440064/136‑1 (MQ=255) gAGGTGTTTGCCACAGGATTGTTACAGATTCTGTCAAAACACTTTATCAAAACACCCACTGTTTCGGCCTTGGCCAGCGCCATAAAATTCACCTCGCCAGCAACGGCAAGGCGATTAACAACTGGTAACAGccccc < 1:436624/136‑1 (MQ=255) | GTTGTTTTTGTATGGAACCGTTTGTAACTGTACCAGAGCGACAAAAGCCATTCGACGTTGGTAGTAATTTGGTACACCCGTTCCGGCAAAACGCTCTGGCGCGGGCATTACAGCCTTGCCGACGAAAAAACGAGGGGTTTGCCACAGGATTGTTACAGATTCTGTCAAAACACTTTATCAAAACACCCACTGTTTCGGCCTTGGCCAGCGCCATAAAATTCACCTCGCCAGCAACGGCAAGGCGATTAACAACTGGTAACAGCCCCC > NC_002947/5104281‑5104547 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |